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Paddy soil, compost, kitchen waste and landfill waste were used to develop the microbial inoculum for the enhancement of biogas production from cattle dung. Fourteen digesters were set up and digestion was carried out in batch type manner for eight weeks. The samples were analyzed for dehydrogenase activity and cellulase activity. Biogas production was measured for a period of eight weeks. The maximum biogas production (32.1 litres) was observed at the end of 4th week on supplementation of cattle dung with kitchen waste @ 5%, compost @ 5%, landfill waste @ 5% and paddy soil @ 5% on dry weight basis in batch anaerobic digestion. The dehydrogenase activity (1772.0 μg TPF/g sample/24 h) and cellulase activity (178.7 μg glucose/g sample/24 h) was observed at 4th week in digester 14.