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The use of pesticides used in the agricultural sector for pest control has caused the deterioration of Colombian arable soils and environmental problems due to their excessive use. As a consequence, interest has arisen in the use of biological agents as an alternative method for disease control. Through qualitative research, literature review and implementation of a survey of 65 farmers in the municipality of Monterrey Casanare as an information collection technique, the objective of this article is answered, which consisted of determining the biological control techniques that exert a antagonistic against the control of phytopathogenic fungi, in agricultural crops in the municipality of Monterrey Casanare. Likewise, it is intended to design a protocol where each of the phases that must be implemented for the use of antagonistic microorganisms in the biological control of phytopathogenic organisms for the study area are observed. The results obtained reflect a high use of chemical methods by farmers that affect their well-being and the environment, likewise, with regard to the selection of strains through environmental, economic and technical criteria, it was obtained that Trichoderma harzianum Rifai is the strain that best fits the selection criteria and the study area. [ABSTRACT FROM AUTHOR]