1. Comprehensive two-dimensional gas chromatography combined to multivariate data analysis for detection of disease-resistant clones of Eucalyptus
- Author
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Bruna R. Toledo, Edson Luiz Furtado, Leandro Wang Hantao, Marília Pizetta, Fabiana Alves de Lima Ribeiro, Caroline Geraldi Pierozzi, and Fabio Augusto
- Subjects
Analyte ,Multivariate analysis ,Chemistry(all) ,Analytical chemistry ,Metabolic profile ,Resistance biomarkers ,Mass spectrometry ,Solid-phase microextraction ,Rust ,Gas Chromatography-Mass Spectrometry ,Analytical Chemistry ,Plant Immunity ,Puccinia psidii ,Solid Phase Microextraction ,Plant Diseases ,Eucalyptus ,Volatile Organic Compounds ,Chromatography ,biology ,Chemistry ,Chimera ,food and beverages ,biology.organism_classification ,Plant Leaves ,Multivariate Analysis ,Parallel factor analysis ,Gas chromatography ,Biomarkers ,Comprehensive two-dimensional gas chromatography - Abstract
In this paper it is reported the use of the chromatographic profiles from volatile fractions of plant clones – in this case, hybrids of Eucalyptus grandis×Eucalyptus urophylla – to determine specimens susceptible to rust disease. The analytes were isolated by headspace solid phase microextraction (HS-SPME) and analyzed by comprehensive two-dimensional gas chromatography combined to fast quadrupole mass spectrometry (GC×GC-qMS). Parallel Factor Analysis (PARAFAC) was employed for estimate the correlation between the chromatographic profiles and resistance against Eucalyptus rust, after preliminary variable selection performed by Fisher ratio analysis. The proposed method allowed the differentiation between susceptible and non-susceptible clones and determination of three resistance biomarkers. This approach can be a valuable alternative for the otherwise time-consuming and labor-intensive methods commonly used.
- Published
- 2013
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