1. Electrochemical assay of acetamiprid in vegetables based on nitrogen-doped graphene/polypyrrole nanocomposites
- Author
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Jiasheng Wang, Decheng Zhang, Keke Xu, Ni Hui, and Dongwei Wang
- Subjects
Neonicotinoids ,Limit of Detection ,Nitrogen ,Polymers ,Vegetables ,Graphite ,Pyrroles ,Electrochemical Techniques ,Carbon ,Nitrites ,Nanocomposites ,Analytical Chemistry - Abstract
Dual-mode electrochemical aptasensor based on nitrogen-doped graphene (NG) doped with the conducting polymer polypyrrole (PPy) nanocomposite is proposed for the determination of acetamiprid. NG/PPy was electrodeposited onto the glassy carbon electrode (GCE) using cyclic voltammetry technique. NG/PPy/GCE showed outstanding electrocatalytic activity for the oxidation of nitrite due to "active region" induced by the charge redistribution of carbon atoms. The ultrasensitive dual-mode biosensor for acetamiprid could be easily developed by coupling acetamiprid aptamers with the NG/PPy hybrid. The specific binding between acetamiprid and the aptamers resulted in the increase of differential pulse voltammetry (DPV) signal change and the decrease of chronoamperometry (CA) signal, and the concentration of acetamiprid could be measured. The working potentials of DPV and CA were - 0.2 ~ 0.4 V and - 0.4 ~ 0.4 V (vs. SCE), respectively. The dual-mode acetamiprid biosensor showed a wide linear range from 10
- Published
- 2022