11,329 results on '"spectroscopy, fourier transform infrared"'
Search Results
2. Unsupervised Feature Selection by a Genetic Algorithm for Mid-Infrared Spectral Data
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Warda Boutegrabet, Olivier Piot, Dominique Guenot, and Cyril Gobinet
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Spectrophotometry, Infrared ,Spectroscopy, Fourier Transform Infrared ,Cluster Analysis ,Algorithms ,Analytical Chemistry - Abstract
Dimensional reduction of highly multidimensional datasets such as those acquired by Fourier transform infrared spectroscopy (FTIR) is a critical step in the data analysis workflow. To achieve this goal, numerous feature selection methods have been developed and applied in a supervised context, i.e., using a priori knowledge about data usually in the form of labels for classification or quantitative values for regression. For this, genetic algorithms have been largely exploited due to their flexibility and global optimization principle. However, few applications in an unsupervised context have been reported in infrared spectroscopy. The aim of this article is to propose a new unsupervised feature selection method based on a genetic algorithm using a validity index computed from KMeans partitions as a fitness function. Evaluated on a simulated dataset and validated and tested on three real-world infrared spectroscopic datasets, our developed algorithm is able to find the spectral descriptors improving clustering accuracy and simplifying the spectral interpretation of results.
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- 2022
3. Blood serum lipid profiling may improve the management of recurrent miscarriage: a combination of machine learning of mid-infrared spectra and biochemical assays
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Zozan Guleken, Pınar Yalçın Bahat, Ömer Faruk Toto, Huri Bulut, Paweł Jakubczyk, Jozef Cebulski, Wiesław Paja, Krzysztof Pancerz, Agnieszka Wosiak, Joanna Depciuch, İstinye Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Bulut, Huri, and CGN-9704-2022
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Serum ,Abortion, Habitual ,Fourier Transform İnfrared ,Biochemistry ,Analytical Chemistry ,Machine Learning ,Total Protein ,Pregnancy ,Spectroscopy, Fourier Transform Infrared ,Lipid Profile ,Humans ,Recurrent Miscarriage ,Female ,Prospective Studies ,Oxidative Load ,Triglycerides - Abstract
The present article is focused on developing and validating an efficient, credible, minimally invasive technique based on spectral signatures of blood samples of women with recurrent miscarriage vs. those of healthy individuals who were followed in the Department of Obstetrics and Gynecology for 2 years. For this purpose, blood samples from a total of 120 participants, including healthy women (n=60) and women with diagnosed recurrent miscarriage (n=60), were obtained. The lipid profile (high-density lipoprotein, low-density lipoprotein, triglyceride, and total cholesterol levels) and lipid peroxidation (malondialdehyde and glutathione levels) were evaluated with a Beckman Coulter analyzer system for chemical analysis. Biomolecular structure and composition were determined using an attenuated total reflectance sampling methodology with Fourier transform infrared spectroscopy alongside machine learning technology to advance toward clinical translation. Here, we developed and validated instrumentation for the analysis of recurrent miscarriage patient serum that was able to differentiate recurrent miscarriage and control patients with an accuracy of 100% using a Fourier transform infrared region corresponding to lipids. We found that predictors of lipid profile abnormalities in maternal serum could significantly improve this patient pathway. The study also presents preliminary results from the first prospective clinical validation study of its kind. WOS:000867589600001 Q2
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- 2022
4. Direct Construction of Peaks from Free Induction Decay Curves for Gas Chromatography–Molecular Rotational Resonance Spectroscopy without Fourier Transforms
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M. Farooq Wahab, Justin L. Neill, and Daniel W. Armstrong
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Chromatography, Gas ,Fourier Analysis ,Spectroscopy, Fourier Transform Infrared ,Algorithms ,Analytical Chemistry - Abstract
The concept of coupling gas chromatography with molecular rotational resonance spectroscopy (GC-MRR) was introduced in 2020, combining the separation capabilities of GC with the unparalleled specificity of MRR. In this study, we address the challenge of the high data throughput of MRR spectrometers, as GC-MRR spectrometers can generate thousands to millions of data points per second. In the previous GC-MRR studies, a free induction decay (FID) measurement was Fourier transformed to generate each point on the chromatogram. Such extensive calculations limit the performance, sensitivity, and speed of GC-MRR. A direct approach is proposed here to extract peak intensity from FID using the Gram-Schmidt vector orthogonalization method. First, analyte-free FIDs are used to construct a basis set representing the instrument's background noise, and then the remaining FIDs are orthogonalized to this fixed basis set. Each FID yields a single intensity value after Gram-Schmidt orthogonalization. The magnitude of the orthogonalized analyte FID is the signal intensity plotted in the chromatogram. This approach is computationally much faster (up to 10 times) than the conventional Fourier transform algorithm, is at least as sensitive as the FT algorithm, and maintains or improves the chromatographic peak shape. We compare the sensitivity, linearity, and chromatographic peak shapes for the Fourier transform and Gram-Schmidt approaches using both synthetically generated FIDs and instrumental data. This approach would allow the summed peak intensity to be displayed essentially in real-time, following which identified peaks can be further investigated to identify and quantify the species associated with each.
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- 2022
5. “One-pot” preparation of aminated carbon nanotube-modified magnetic nanoparticles and their application to the quantification of phenoxyacetic acid herbicides in cereal and vegetable samples
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Youfang, Huang, Jun, Liu, and Xiaojia, Huang
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Acetonitriles ,Formates ,Herbicides ,Nanotubes, Carbon ,General Chemical Engineering ,Organic Chemistry ,Acetates ,Ferric Compounds ,Biochemistry ,Analytical Chemistry ,2-Propanol ,Spectroscopy, Fourier Transform Infrared ,Vegetables ,Solvents ,Electrochemistry ,Humans ,Ferrous Compounds ,Edible Grain ,Magnetite Nanoparticles ,Hydrogen - Abstract
Phenoxyacetic acid herbicides (PAs) are widely used to control the growth of broad-leaf weeds in corn, tobacco, etc. The presence of PAs in plants even at low concentrations (at the ng/L to μg/L scale) may induce severe effects and lead to human health risks. Hence, a sensitive and reliable method for the determination of PAs at trace levels in cereals and vegetables is highly desired. Magnetic solid-phase extraction (MSPE) has attracted considerable attention on account of its benefits such as ease of separation, less solvent consumption, and good service life. In this study, aminated carbon nanotube-modified magnetic nanoparticles (NH
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- 2022
6. Rapid Detection and Determination of Scopolamine in the Leaf Extract of Black Henbane (Hyoscyamus niger L.) Plants Using a Novel Nanosensor
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Fatemeh Sadat Mousavizadeh, Nahid Sarlak, Mansour Ghorbanpour, and Reza Ghafarzadegan
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Pharmacology ,Ethanol ,Plant Extracts ,Scopolamine ,Reproducibility of Results ,Parasympatholytics ,Cholinergic Antagonists ,Citric Acid ,Hyoscyamus ,Analytical Chemistry ,Spectroscopy, Fourier Transform Infrared ,Environmental Chemistry ,Cellulose ,Agronomy and Crop Science ,Tropanes ,Food Science - Abstract
Background Scopolamine is among the most essential tropane alkaloids used to remedy various nervous system disorders such as urinary incontinence, motion sickness, and spasmodic movements because of its anticholinergic and antispasmodic effects. Objective In this study, an optical nanosensor was fabricated using nano-Dragendorff’s reagent to detect and determine scopolamine in different plant parts at different stages of growth. Method For fabrication of the sensing phase, GO-g-PCA/DR was synthesized by encapsulation of Dragendorff’s reagent (DR) on the graphene oxide grafted with poly citric acid (GO-g-PCA) with ultrasonication for 15 min and stirred for 80 min at room temperature, and then it was immobilized on a triacetyl cellulose membrane. The kinetic absorption profiles were recorded at 360 nm, which is concerned with the reaction between immobilized GO-g-PCA/DR and different concentrations of scopolamine. Results The nanosensor showed a rapid, strong, and stable response to the scopolamine solution with changing the absorption spectrum at 360 nm. The reaction was completed in a period of 300 s. The SEM, AFM, and FT-IR analysis of nanocomposites and nanosensors show the successful synthesis of GO-g-PCA/DR and the reaction between nanosensor and scopolamine. All experiments were performed at the wavelength of 360 nm, room temperature, pH 7 (the scopolamine solution pH), and 300 s. The nanosensor had a linear range of 0.65 to 19.63 μg/mL and 0.19 ± 0.025 μg/mL as the limit of detection for scopolamine determination. In order to reuse the designed nanosensor, it was recovered with ethanol, and the color ultimately returned to its original state. Conclusions This in situ nanosensor can determine the scopolamine in real samples with easy reversibility, extended lifetime, and reproducibility of the sensing phase response. Highlights A sensitive, precise, and fast response optical nanosensor is designed for in situ determination of scopolamine in real samples.
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- 2022
7. Quantum Cascade Laser-Based Vibrational Circular Dichroism Augmented by a Balanced Detection Scheme
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Daniel R. Hermann, Georg Ramer, Markus Kitzler-Zeiler, and Bernhard Lendl
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Fourier Analysis ,Circular Dichroism ,Spectroscopy, Fourier Transform Infrared ,Lasers, Semiconductor ,Analytical Chemistry - Abstract
Vibrational circular dichroism (VCD) constitutes a powerful technique, enabling the determination of the absolute configuration of molecules without the need for specialized reagents. While delivering critical information, VCD signals commonly are several orders of magnitude weaker than classical absorbance signals, which so far necessitated long measurement times to achieve acceptable signal-to-noise ratios (SNRs) in VCD experiments. We present here an improved setup for the measurement of VCD in the range between 5.6 and 6.5 μm. Employing an external cavity quantum cascade laser (EC-QCL) as a high-power light source, we collected spectra with competitive noise levels in less than 5 min. The basis for this improvement was a balanced detection module combined with an optical path catered to VCD measurements. With the stabilization provided by the two-detector setup, noise originating from the laser source could be suppressed effectively. Noise level improvement up to a factor of 4 compared to the classical single detector EC-QCL-VCD could be reported. Compared to commercial Fourier transform infrared (FT-IR) instruments, the presented setup offers measurement time reductions of a factor of at least 6, with comparable noise levels. The applicability of the setup for qualitative and quantitative VCDs was proven. With the comparatively high temporal resolution provided, the monitoring of optically active processes will be possible in future applications.
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- 2022
8. Mass spectrometry and synchrotron‐FTIR microspectroscopy reveal the anti‐inflammatory activity of Bua Bok extracts
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Atchara Paemanee, Siriluk Rattanabunyong, Yanisa Ketngamkum, Jeeraprapa Siriwaseree, Ponkanok Pongpamorn, Kanokwan Romyanon, Sithichoke Tangphatsornruang, Buabarn Kuaprasert, and Kiattawee Choowongkomon
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Lipopolysaccharides ,Diclofenac ,Ethanol ,Plant Extracts ,Anti-Inflammatory Agents ,Plant Science ,General Medicine ,Nitric Oxide ,Biochemistry ,Mass Spectrometry ,Analytical Chemistry ,Centella ,Complementary and alternative medicine ,Phospholipases ,Nitriles ,Spectroscopy, Fourier Transform Infrared ,Drug Discovery ,Molecular Medicine ,Synchrotrons ,Food Science - Abstract
Bua Bok or Centella asiatica (CA) is an Asian vegetable with anti-inflammatory benefits. Asiaticoside, asiatic acid, madecassoside and madecassic have been characterised as major active ingredients with a wide range of pharmacological advantages. In manufacturing processes, high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LCMS) are used to routinely determine the active compounds in raw materials.This research aims to explore anti-inflammatory properties, characterise metabolites and observe the biochemical changes of the inflammatory induced macrophages after pretreatment with the potential extracted fractions.Bua Bok leaf extracts were prepared. Macrophages were pretreated with non-toxic fractions to determine the anti-inflammatory action. Tentative metabolites of effective fractions were identified by LC-MS. Synchrotron fourier-transform infrared (S-FTIR) microspectroscopy was utilised to observe the biochemical change of the lipopolysaccharide (LPS)-induced cells after pretreatment with potential fractions.Fractions of ethyl acetate, 30% and 100% ethanol highly increased the nitrile scavenging and suppressed the function of phospholipase AWhile the anti-inflammatory actions of 70% and 100% ethanol were similar. S-FTIR expressed they inhibited inflammatory response with the distinct features of biomolecules. The S-FTIR, LC-MS and biological assay confidently provided the efficient strategies to inform the advantage of herbal extract on cellular organisation instead of a single compound.
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- 2022
9. Anticoagulant activity analysis and origin identification of Panax notoginseng using HPLC and ATR‐FTIR spectroscopy
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Zhi‐Ying Cui, Chun‐Lu Liu, Dan‐Dan Li, Yuan‐Zhong Wang, and Fu‐Rong Xu
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China ,Anticoagulants ,Panax notoginseng ,Plant Science ,General Medicine ,Saponins ,Biochemistry ,Analytical Chemistry ,Complementary and alternative medicine ,Spectroscopy, Fourier Transform Infrared ,Drug Discovery ,Molecular Medicine ,Chromatography, High Pressure Liquid ,Food Science - Abstract
Panax notoginseng is one of the traditional precious and bulk-traded medicinal materials in China. Its anticoagulant activity is related to its saponin composition. However, the correlation between saponins and anticoagulant activities in P. notoginseng from different origins and identification of the origins have been rarely reported.We aimed to analyze the correlation of components and activities of P. notoginseng from different origins and develop a rapid P. notoginseng origin identification method.Pharmacological experiments, HPLC, and ATR-FTIR spectroscopy (variable selection) combined with chemometrics methods of P. notoginseng main roots from four different origins (359 individuals) in Yunnan Province were conducted.The pharmacological experiments and HPLC showed that the saponin content of P. notoginseng main roots was not significantly different. It was the highest in main roots from Wenshan Prefecture (9.86%). The coagulation time was prolonged to observe the strongest effect (4.99 s), and the anticoagulant activity was positively correlated with the contents of the three saponins. The content of ginsenoside RgThis method is a powerful analytical tool for the activity analysis and identification of Chinese medicinal materials from different origins.
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- 2022
10. Preparation and chromatographic performance of cardanol-bonded silica stationary phase
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Lei, Zeng, Lijuan, Jiang, Xingdong, Yao, Ting, Wang, Bo'an, Shi, and Fuhou, Lei
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Phenols ,Plant Extracts ,General Chemical Engineering ,Spectroscopy, Fourier Transform Infrared ,Organic Chemistry ,Electrochemistry ,Camptothecin ,Polycyclic Aromatic Hydrocarbons ,Silicon Dioxide ,Hydrophobic and Hydrophilic Interactions ,Biochemistry ,Chromatography, High Pressure Liquid ,Analytical Chemistry - Abstract
As green, less toxic, widely available, and site-rich functional ligands, natural products are widely used for the development of chromatographic stationary phases. In this work, a novel stationary phase, cardanol-bonded on silica (CBS) was prepared using
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- 2022
11. Synthesis of a Novel Electrochemical Probe for the Sensitive and Selective Detection of Biothiols and Its Clinical Applications
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Islam M. Mostafa, Hongzhan Liu, Saima Hanif, Muhammad Rehan Hasan Shah Gilani, Yiran Guan, and Guobao Xu
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Spectrometry, Fluorescence ,Spectroscopy, Fourier Transform Infrared ,Humans ,Cysteine ,Sulfhydryl Compounds ,Glutathione ,Homocysteine ,Fluorescent Dyes ,Analytical Chemistry - Abstract
The ability to estimate and quantify biothiols in biological fluids is very significant for attaining a detailed understanding of biothiols-related pathological diseases. Most of the developed methods for biothiols detection are not suitable for this purpose owing to their low sensitivity, poor selectivity, and long experimental procedures. In this study, a novel and simple structure electrochemical probe has been synthesized for the first time for the selective determination of biothiols. The developed probe is based on using 2,4-dinitrobenzenesulfonyl moiety (DNBS) as a selective recognition moiety for biothiols. The electrochemical probe was successfully fabricated through a facile one-step reaction between 2,4-dinitrobenzenesulfonyl chloride (DNBS-Cl) and
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- 2022
12. Simultaneous determination of 76 pesticide residues in the traditional Chinese medicine by magnetic hydrophilic-lipophilic-balanced materials assisted matrix solid phase dispersion extraction-high performance liquid chromatography-tandem mass spectrometry
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Dan, Wei and Ming, Guo
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Tandem Mass Spectrometry ,Magnetic Phenomena ,General Chemical Engineering ,Spectroscopy, Fourier Transform Infrared ,Organic Chemistry ,Pesticide Residues ,Electrochemistry ,Medicine, Chinese Traditional ,Biochemistry ,Chromatography, High Pressure Liquid ,Analytical Chemistry - Abstract
Traditional Chinese medicine (TCM) is gaining popularity worldwide, but its quality is often affected by excessive pesticide residues during cultivation and production. A sensitive and reliable method for the simultaneous determination of multi-residue pesticides in TCMs is the key to guarantee the quality and safety of TCMs. In this study, broad-spectrum hydrophilic-lipophilic balanced magnetic adsorbents were prepared for the magnetic matrix solid phase dispersion (MMSPD) extraction of 76 pesticides from three different TCMs before their detection by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Unlike the traditional matrix solid phase dispersion (MSPD), which required tedious SPE column packing, an external magnetic field was employed in our method for magnetic isolation and extraction from TCM samples, followed by grounding adsorption. First, broad-spectrum hydrophilic-lipophilic balanced magnetic adsorbents, Fe
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- 2022
13. Preparation and application of porous organic cage capillary electrochromatographic chiral column
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Wenyan, Jia, Minghua, Tang, Junhui, Zhang, and Liming, Yuan
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Capillary Electrochromatography ,General Chemical Engineering ,Spectroscopy, Fourier Transform Infrared ,Organic Chemistry ,Electrochemistry ,Stereoisomerism ,Silicon Dioxide ,Porosity ,Biochemistry ,Analytical Chemistry - Abstract
Capillary electrochromatography for enantioseparation has received considerable research attention in the past decades, because it integrates the advantages of classical electrophoresis and modern micro-column separation. Chirality is a fundamental feature of compounds found in nature and is also a major concern in the modern pharmaceutical industry. Porous organic cages (POCs) are defined as a class of porous materials with permanent ordered three-dimensional cavity structures that are different from those of porous materials, such as zeolite, metal-organic frameworks, covalent organic frameworks, and mesoporous silica. POCs have good solubility in general organic solvents and can be used as a chromatographic stationary phase conveniently coated inside a standard capillary column. Homochiral POCs with hydroxyl groups on the cage molecules were synthesized by imine-linked condensation of 2-hydroxy-1,3,5-triformylbenzene with (1
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- 2022
14. Fast Burst-Sparsity Learning-Based Baseline Correction (FBSL-BC) Algorithm for Signals of Analytical Instruments
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Haoran Li, Suyi Chen, Jisheng Dai, Xiaobo Zou, Tao Chen, Tianhong Pan, and Melvin Holmes
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Spectroscopy, Fourier Transform Infrared ,Bayes Theorem ,Algorithms ,Analytical Chemistry - Abstract
Baseline correction is a critical step for eliminating the interference of baseline drift in spectroscopic analysis. The recently proposed sparse Bayesian learning (SBL)-based method can significantly improve the baseline correction performance. However, it has at least two disadvantages: (i) it works poorly for large-scale datasets and (ii) it completely ignores the burst-sparsity structure of the sparse representation of the pure spectrum. In this paper, we present a new fast burst-sparsity learning method for baseline correction to overcome these shortcomings. The first novelty of the proposed method is to jointly adopt a down-sampling strategy and construct a multiple measurements block-sparse recovery problem with the down-sampling sequences. The down-sampling strategy can significantly reduce the dimension of the spectrum; while jointly exploiting the block sparsity among the down-sampling sequences avoids losing the information contained in the original spectrum. The second novelty of the proposed method is introducing the pattern-coupled prior into the SBL framework to characterize the inherent burst-sparsity in the sparse representation of spectrum. As illustrated in the paper, burst-sparsity commonly occurs in peak zones with more denser nonzero coefficients. Properly utilizing such burst-sparsity can further enhance the baseline correction performance. Results on both simulated and real datasets (such as FT-IR, Raman spectrum, and chromatography) verify the substantial improvement, in terms of estimation accuracy and computational complexity.
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- 2022
15. Noninvasive Diagnostic for COVID-19 from Saliva Biofluid via FTIR Spectroscopy and Multivariate Analysis
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Márcia H. C. Nascimento, Wena D. Marcarini, Gabriely S. Folli, Walter G. da Silva Filho, Leonardo L. Barbosa, Ellisson Henrique de Paulo, Paula F. Vassallo, José G. Mill, Valério G. Barauna, Francis L. Martin, Eustáquio V. R. de Castro, Wanderson Romão, and Paulo R. Filgueiras
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SARS-CoV-2 ,Multivariate Analysis ,Spectroscopy, Fourier Transform Infrared ,COVID-19 ,Discriminant Analysis ,Humans ,Least-Squares Analysis ,Saliva ,Article ,Analytical Chemistry - Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the worst global health crisis in living memory. The reverse transcription polymerase chain reaction (RT-qPCR) is considered the gold standard diagnostic method, but it exhibits limitations in the face of enormous demands. We evaluated a mid-infrared (MIR) data set of 237 saliva samples obtained from symptomatic patients (138 COVID-19 infections diagnosed via RT-qPCR). MIR spectra were evaluated via unsupervised random forest (URF) and classification models. Linear discriminant analysis (LDA) was applied following the genetic algorithm (GA-LDA), successive projection algorithm (SPA-LDA), partial least squares (PLS-DA), and a combination of dimension reduction and variable selection methods by particle swarm optimization (PSO-PLS-DA). Additionally, a consensus class was used. URF models can identify structures even in highly complex data. Individual models performed well, but the consensus class improved the validation performance to 85% accuracy, 93% sensitivity, 83% specificity, and a Matthew’s correlation coefficient value of 0.69, with information at different spectral regions. Therefore, through this unsupervised and supervised framework methodology, it is possible to better highlight the spectral regions associated with positive samples, including lipid (∼1700 cm–1), protein (∼1400 cm–1), and nucleic acid (∼1200–950 cm–1) regions. This methodology presents an important tool for a fast, noninvasive diagnostic technique, reducing costs and allowing for risk reduction strategies.
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- 2022
16. Novel Method for Extracting the Spectrum of a Supramolecular Complex via a Comprehensive Approach Involving Two-Dimensional Correlation Spectroscopy, Genetic Algorithm, and Grid Searching
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An-Qi He, Zhen-Qiang Yu, Jun Song, Li-Min Yang, Yi-Zhuang Xu, Isao Noda, and Yukihiro Ozaki
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Solutions ,Research Design ,Spectroscopy, Fourier Transform Infrared ,Algorithms ,Protein Structure, Secondary ,Analytical Chemistry - Abstract
A supramolecular complex may be formed by two solutes via a weak intermolecular interaction in a solution. The spectrum of the complex is often inundated by the spectra of the solutes that are not involved in the intermolecular interaction. Herein, a novel spectral analysis approach is proposed to retrieve the spectrum of the supramolecular complex. First, a two-dimensional (2D) asynchronous spectrum is constructed. Then, a genetic algorithm is used to obtain a heuristic spectrum of the supramolecular complex. The heuristic spectrum is a linear combination of the spectrum of the complex and the spectrum of a solute. The coefficients of the linear combination are then obtained, according to which the equilibrium constants are invariant among the sample solutions used to construct the 2D asynchronous spectrum. We have applied the approach to a supramolecular system formed by benzene and I
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- 2022
17. Association between ageing, brain chemistry and white matter volume revealed with complementary MRI and FTIR brain imaging
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Virginie Lam, Juliette Phillips, Elizabeth Harrild, Rebecca J. Tidy, Ashley L. Hollings, Lincoln Codd, Kirsty Richardson, Liesl Celliers, Ryu Takechi, John C. L. Mamo, and Mark J. Hackett
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Brain Chemistry ,Aging ,Fourier Analysis ,Brain ,Neuroimaging ,White Matter ,Magnetic Resonance Imaging ,Biochemistry ,Analytical Chemistry ,Mice ,Spectroscopy, Fourier Transform Infrared ,Electrochemistry ,Animals ,Environmental Chemistry ,Spectroscopy - Abstract
Ex vivo FTIR imaging reveals lipid distributions (red) alongside other spectroscopic markers in brain tissue, which can be used to provide greater biochemical insight when combined with in vivo MRI data sets collected from the same brain.
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- 2022
18. Nondestructive assessment of tissue engineered cartilage based on biochemical markers in cell culture media: application of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy
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William Querido, Sabrina Zouaghi, Mugdha Padalkar, Justin Morman, Jessica Falcon, Shital Kandel, and Nancy Pleshko
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Fourier Analysis ,Tissue Engineering ,Cell Culture Techniques ,food and beverages ,Biochemistry ,Article ,Culture Media ,Analytical Chemistry ,Cartilage ,Glucose ,Spectroscopy, Fourier Transform Infrared ,Electrochemistry ,Environmental Chemistry ,Lactic Acid ,Biomarkers ,Spectroscopy - Abstract
Tissue engineering of cartilage for tissue repair has many challenges, including the inability to assess when the developing construct has reached compositional maturity for implantation. The goal of this study was to provide a novel analytical approach to nondestructively assess tissue engineered cartilage (TEC) during in vitro development. We applied attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to establish a quick and straightforward method to evaluate consumption of glucose and secretion of the metabolite lactate in the culture media, processes that are associated with tissue development. Using a series of standards, we showed by principal component analysis (PCA) that ATR-FTIR data was able to distinguish culture media with varying amounts of glucose and lactate. The 2(nd) derivative spectra displayed specific peaks of glucose at 1035 cm(−1) and lactate at 1122 cm(−1), and both the spectral first principal component (PC-1) scores and the 1122/1035 peak ratio very strongly correlated with the concentration of these components. TEC was prepared using chondrogenic cells grown in hydrogels, and analyzed for cell viability, distribution, and formation of proteoglycan (PG, a major cartilage protein). ATR-FTIR data of the cell culture media harvested during TEC development showed that the spectral PC-1 and the 1122/1035 peak ratio could significantly distinguish cultures with different amounts of constructs (1, 3 or 5 constructs/well) or with constructs at different developmental stages (3 or 5 weeks of culture). Interestingly, we also found that the PG content of the TEC was significantly correlated with both spectral PC-1 (r=−0.79) and the 1122/1035 peak ratio (r=0.80). Therefore, monitoring relative glucose and lactate concentrations in cell culture media by ATR-FTIR provides a novel nondestructive approach to assess development of TEC.
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- 2022
19. An efficient 3D adsorbent foam based on graphene oxide/AgO nanoparticles for rapid vortex-assisted floating solid phase extraction of bisphenol A in canned food products
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Mahboube Shirani, Abolfazl Aslani, Soheila Sepahi, Ehsan Parandi, Azadeh Motamedi, Elham Jahanmard, Hamid Rashidi Nodeh, and Behrouz Akbari-adergani
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Time Factors ,General Chemical Engineering ,Solid Phase Extraction ,General Engineering ,Metal Nanoparticles ,Hydrogen-Ion Concentration ,Fluorescence ,Analytical Chemistry ,Phenols ,Food, Preserved ,Spectroscopy, Fourier Transform Infrared ,Microscopy, Electron, Scanning ,Graphite ,Adsorption ,Benzhydryl Compounds ,Chromatography, High Pressure Liquid - Abstract
In this study, a three-dimensional adsorbent was developed based on graphene oxide/AgO nanoparticles over interconnected nickel foam (GO/AgO@Ni foam) for rapid and efficient vortex assisted floating solid phase extraction of bisphenol A in canned food products prior to high performance liquid chromatography with a fluorescence detector. The analytical techniques scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX) and Fourier transform infrared (FT-IR) were used for characterization of the synthetized GO/AgO@Ni foam. The effect of proficiency factors including pH, foam size, vortexing time, salt addition, sample volume, desorption type and volume, and desorption time on the extraction efficiency of bisphenol A were explored through the matrix match method. Under the above experimental conditions, the figures of merit of the method were acquired as LODs (S/N = 3) of 0.18-0.84 μg kg
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- 2022
20. A high-performance electrochemical sensor for sensitive detection of tetracycline based on a Zr-UiO-66/MWCNTs/AuNPs composite electrode
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Xueyu Weng, Jingqi Huang, Huazhu Ye, He Xu, Dongqing Cai, and Dongfang Wang
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Nanotubes, Carbon ,General Chemical Engineering ,Spectroscopy, Fourier Transform Infrared ,Phthalic Acids ,General Engineering ,Metal Nanoparticles ,Gold ,Prospective Studies ,Tetracycline ,Electrodes ,Metal-Organic Frameworks ,Anti-Bacterial Agents ,Analytical Chemistry - Abstract
Herein, a high-performance electrochemical sensor was constructed based on a metal-organic framework (Zr-UiO-66)/multi-carbon nanotubes/gold nanoparticles (Zr-UiO-66/MWCNTs/AuNPs) composite modified glassy carbon electrode for sensitive determination of tetracycline. The morphology, structure, and performance of Zr-UiO-66/MWCNTs/AuNPs were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FT-IR), and electrochemical techniques. The Zr-UiO-66/MWCNTs/AuNPs nanohybrids exhibited excellent electrocatalytic activity towards the oxidation of tetracycline, mainly because of the synergistic effect of the MOFs, MWCNTs, and gold nanoparticles. The electrochemical kinetics and catalytical mechanism of tetracycline were demonstrated, proving that tetracycline's electrocatalytic oxidation reaction was an absorption-controlled two-step process involving the transfer of two protons and two electrons, respectively. Furthermore, a simple and facile method was used to achieve the regeneration of the absorbed saturated electrode. A low concentration of tetracycline was detected by amperometry with the linear ranges of 5 × 10
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- 2022
21. A mesoporous silica-based probe with a molecularly imprinted polymer recognition and Mn:ZnS QDs@rhodamine B ratiometric fluorescence sensing strategy for the analysis of 4-nitrophenol
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Jiao Luo, Hongyan Tan, Bin Yang, Donghua Chen, and Junjie Fei
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Manganese ,Polymers ,Rhodamines ,General Chemical Engineering ,General Engineering ,Water ,Sulfides ,Silicon Dioxide ,Analytical Chemistry ,Molecular Imprinting ,Nitrophenols ,Spectrometry, Fluorescence ,Manganese Compounds ,Molecularly Imprinted Polymers ,Zinc Compounds ,Spectroscopy, Fourier Transform Infrared - Abstract
In this study, a mesoporous silica fluorescence probe co-doped with manganese-doped zinc sulfide quantum dots (Mn:ZnS QDs) and rhodamine B (RB) and coated with molecularly imprinted polymer (MIP) has been prepared by sol-gel methods. The morphology and structure were characterized in detail by transmission electron microscopy (TEM), Fourier transform-infrared absorption spectroscopy (FT-IR) and ultraviolet-visible absorption spectroscopy (UV-vis). The probe exhibited two characteristic emission peaks at 411 nm and 582 nm, and the synchronous ratiometric fluorescence responses
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- 2022
22. The use of concentrated multiple reflection ATR spectroscopy in the analysis of nail polish as forensic evidence
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Ali Koçak and Sangsu Kang
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Spectroscopy, Fourier Transform Infrared ,Electrochemistry ,Environmental Chemistry ,Poland ,Forensic Medicine ,Biochemistry ,Spectroscopy ,Specimen Handling ,Analytical Chemistry - Abstract
Multiple reflection ATR spectroscopy is a technique used in cases where additional sensitivity is required over that provided by single reflection. Normally, multiple reflection equipment requires significantly more sample in order to adequately cover a larger sampling area. These larger sample amounts may not be available in forensic investigations. In this work, the capabilities of a concentrated multiple reflection ATR (CMRATR) accessory, in which all reflections are confined to a very small area, are demonstrated. Liquid solution sample sizes of only 5 to 15 μl are required. The accessory is used with an evaporative technique which further enhances sensitivity by concentrating the solute in close contact onto the ATR sampling surface. The technique along with a multivariate statistical analysis is used to discriminate visually similar nail polish samples made by different manufacturers.
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- 2022
23. Non-destructive molecular FTIR spectromicroscopy for real time assessment of redox metallodrugs
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Roberto Santana da Silva, Lucyano J. A. Macedo, Fernando P. Rodrigues, Fabio Zobi, Leandro N.C. Máximo, Ayaz Hassan, and Frank N. Crespilho
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chemistry.chemical_classification ,Dynamic spectroscopy ,General Chemical Engineering ,Biomolecule ,General Engineering ,Proteins ,Nanotechnology ,DNA ,Cellular level ,Redox ,Analytical Chemistry ,chemistry ,Metals ,Non destructive ,Spectroscopy, Fourier Transform Infrared ,Drug release ,Oxidation-Reduction - Abstract
Recent emergence of FTIR spectromicroscopy (micro-FTIR) as a dynamic spectroscopy for imaging to the study of biological chemistry has opened new possibilities for investigating in situ drug release, redox chemistry effects with biological molecules, DNA and drug interactions, membranes dynamic, and redox reaction with proteins at the single cell level. Micro-FTIR applied to metallodrugs have been playing important role in the last decade because of its great potential to achieve more robust and controlled pharmacological effects against several diseases, including cancer. An important aspect in the development of these drugs is to understand their cellular properties, such as uptake, accumulation, activity, and toxicity. In this review, we present the potential application of the micro-FTIR and its importance for studying metal-based drugs, highlighting the prospective of chemistry of living cells. We also present a bioimaging emphasis, which is therefore of high importance to localize the cellular processes, for the proper understanding of the mechanism of action.
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- 2022
24. Fusion of a low-cost electronic nose and Fourier transform near-infrared spectroscopy for qualitative and quantitative detection of beef adulterated with duck
- Author
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Fangkai Han, Xingyi Huang, Joshua H. Aheto, Xiaorui Zhang, and Marwan M. A. Rashed
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Ducks ,Spectroscopy, Near-Infrared ,Fourier Analysis ,General Chemical Engineering ,Spectroscopy, Fourier Transform Infrared ,General Engineering ,Animals ,Cattle ,Electronic Nose ,Analytical Chemistry - Abstract
A low-cost electronic nose (E-nose) based on colorimetric sensors fused with Fourier transform-near-infrared (FT-NIR) spectroscopy was proposed as a rapid and convenient technique for detecting beef adulterated with duck. The total volatile basic nitrogen, protein, fat, total sugar and ash contents were measured to investigate the differences of basic properties between raw beef and duck; GC-MS was employed to analyze the difference of the volatile organic compounds emitted from these two types of meat. For variable selection and spectra denoising, the simple
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- 2022
25. Switchable inhibitory behavior of divalent magnesium ion in DNA hybridization-based gene quantification
- Author
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Hyowon Jin, Hyun Jeong Lim, Mark R. Liles, Beelee Chua, and Ahjeong Son
- Subjects
Cations, Divalent ,Spectroscopy, Fourier Transform Infrared ,Electrochemistry ,Nucleic Acid Hybridization ,Environmental Chemistry ,Magnesium ,DNA ,Biochemistry ,Spectroscopy ,Analytical Chemistry - Abstract
Mg2+ ion is a switchable inhibitor that can cause either under or over-estimation at different concentrations in DNA hybridization based gene quantification.
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- 2022
26. Novel two-photon absorption compounds with different organic cations: facile synthesis, photophysical properties, detection of viscosity, and selective imaging of the endoplasmic reticulum in living cells
- Author
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Zhi-Bin Cai, Qiao-Xian Lou, Sheng-Li Li, Li-Jun Chen, Qing Ye, and Yu-Peng Tian
- Subjects
Viscosity ,Cations ,Spectroscopy, Fourier Transform Infrared ,Electrochemistry ,Environmental Chemistry ,Endoplasmic Reticulum ,Biochemistry ,Spectroscopy ,Fluorescent Dyes ,Analytical Chemistry - Abstract
A novel acceptor–π–acceptor molecular rotor for the detection of viscosity was developed, and strategies for the design of ER-targeted two-photon excited fluorescent probes based on organic cations have been enriched.
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- 2022
27. Evaluation of propylene glycol methyl ether as a potential challenge agent for leak detection of liquid and headspace from closed system drug transfer devices using Fourier transform infrared spectroscopy
- Author
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Emily G. Westbrook, Amos Doepke, and Robert P. Streicher
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Propylene Glycols ,Protective Devices ,General Chemical Engineering ,Spectroscopy, Fourier Transform Infrared ,General Engineering ,Gases ,National Institute for Occupational Safety and Health, U.S ,United States ,Article ,Analytical Chemistry - Abstract
Choosing an appropriate surrogate of hazardous drugs for use in testing Closed System Drug-Transfer Devices (CSTDs) is a challenging endeavor with many factors that must be considered. It was suggested that the compound propylene glycol methyl ether (PGME) may meet many of the criteria we considered important in a suitable surrogate. Criteria included sufficient volatility to evaporate from aqueous liquid leaks efficiently, a Henry’s constant which produced sufficient vapor phase concentrations to make headspace leaks detectable, and suitability for detection using a low-cost detection system. We evaluated the measurement of vapors from solutions containing PGME released inside a closed chamber. We present data used to quantify limits of detection, limits of quantification, bias, precision, and accuracy of Fourier Transform Infrared Spectroscopy (FTIR) measurements of vapors from 2.5 M PGME solutions. The effects of ethanol as a component of the PGME solution were also evaluated. Liquid drops of PGME solutions and headspace vapors above PGME solutions were released to simulate leaks from CSTDs. Using a calibration apparatus, an instrumental limit of detection (LOD) of 0.25 ppmv and a limit of quantitation (LOQ) of 0.8 ppmv were determined for PGME vapor. A LOD of 1.1 μL and a LOQ of 3.5 μL were determined for liquid aliquots of 2.5 M PGME solution released in a closed chamber. Accurate quantitation of liquid leaks required complete evaporation of droplets. With the upper end of the useable quantitation range limited by slow evaporation of relatively large droplets and the lower end defined by the method LOQ, the method evaluated in this research had a narrow quantitative range for liquid droplets. Displacement of 45 mL of vial headspace containing PGME vapor is the largest amount expected when using the draft NIOSH testing protocol. Release of an unfiltered 45 mL headspace aliquot within the NIOSH chamber was calculated to produce a concentration of 0.8 ppmv based on the Henry’s constant, which is right at the instrumental LOQ. Therefore, the sensitivity of the method was not adequate to determine leaks of PGME vapor from a headspace release through an air filtering CSTD when using the draft NIOSH testing protocols with an FTIR analyzer.
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- 2022
28. Phytic acid determination in food products using the extract of rice sprout and SBA@DABCO nanoparticle-modified filter paper as a novel electrochemical biosensor
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Mohadeseh Rahimi-Mohseni, Jahan Bakhsh Raoof, Tahereh A. Aghajanzadeh, and Reza Ojani
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Phytic Acid ,General Chemical Engineering ,Flour ,General Engineering ,Oryza ,Biosensing Techniques ,Electrochemical Techniques ,Piperazines ,Phosphates ,Analytical Chemistry ,Spectroscopy, Fourier Transform Infrared ,Nanoparticles ,Graphite ,Triticum - Abstract
We outline the fabrication of a highly sensitive biosensor for phytic acid (PA) determination using the extract of rice sprout and SBA@DABCO nanoparticles. The nanoparticles were first added to the surface of small paper disks. After drying, the extract of rice sprout in phosphate buffered saline was immobilized on the paper disks. FT-IR spectroscopy, XRD, and SEM were employed for the characterization of the nanoparticles. The assembly process of the modified paper disks on top of graphite screen-printed electrodes was investigated by cyclic voltammetry and electrochemical impedance spectroscopy. PA measurements were performed by differential pulse voltammetry (DPV) with a small amount of sample (7 μL) for analysis. The cooperation of the rice sprout extract and the nanostructure of this biosensor led to the selective and sensitive electrochemical determination of PA. The limit of detection (S/N = 3) and linear dynamic range for PA determination by DPV were obtained at 0.04 μM and 0.1-10.0 μM, respectively. The biosensor showed excellent sensitivity for the determination of PA in real samples such as biscuits and wheat flour.
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- 2022
29. A peroxyoxalate chemiluminescence recovery system based on the interaction of N-doped graphene oxide nanosheets and an oligopeptide for ultra-sensitive and selective copper(<scp>ii</scp>) ion detection
- Author
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Hongyan Tan, Hong Zhou, and Donghua Chen
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Oxalates ,Luminescence ,General Chemical Engineering ,Luminescent Measurements ,Spectroscopy, Fourier Transform Infrared ,General Engineering ,Water ,Graphite ,Hydrogen Peroxide ,Oligopeptides ,Copper ,Analytical Chemistry - Abstract
In this paper, a peroxyoxalate chemiluminescence (CL) recovery system based on the interaction of N-doped graphene oxide nanosheets (N-GONs) and an oligopeptide for copper(II) ion detection has been reported. N-GONs as an excellent CL enhancer are prepared by the hydrothermal method using citric acid and ammonia, and the morphology and structure are characterized in detail by TEM, XPS, FT-IR and UV/vis
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- 2022
30. Rapid lignin quantification for fungal wood pretreatment by ATR-FTIR spectroscopy
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Nikolett Wittner, János Slezsák, Waut Broos, Jordi Geerts, Szilveszter Gergely, Siegfried E. Vlaeminck, and Iris Cornet
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Chemistry ,Fourier Analysis ,Hydrolysis ,Spectroscopy, Fourier Transform Infrared ,Lignin ,Wood ,Instrumentation ,Engineering sciences. Technology ,Spectroscopy ,Atomic and Molecular Physics, and Optics ,Analytical Chemistry - Abstract
Lignin determination in lignocellulose with the conventional two-step acid hydrolysis method is highly laborious and time-consuming. However, its quantification is crucial to monitor fungal pretreatment of wood, as the increase of acid-insoluble lignin (AIL) degradation linearly correlates with the achievable enzymatic saccharification yield. Therefore, in this study, a new attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy method was developed to track fungal delignification in an easy and rapid manner. Partial least square regression (PLSR) with cross-validation (CV) was applied to correlate the ATR-FTIR spectra with the AIL content (19.9%–27.1%). After variable selection and normalization, a PLSR model with a high coefficient of determination (𝑅2 = 0.87) and a low root mean square (RMSECV = 0.60%) were obtained despite the heterogeneous nature of the fungal solid-state fermentation. These results show that ATR-FTIR can reliably predict the AIL content in fungus-treated wood while being a high-throughput method. This novel method can facilitate the transition to the woodbased economy.
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- 2023
31. Secondary structure alterations of RBC assessed by FTIR-ATR in correlation to 2,3-DPG levels in ApoE/LDLR–/– Mice
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Bulat, Katarzyna, Alcicek, Fatih Celal, Blat, Aneta, Rutkowska, Wiktoria, Szczesny-Malysiak, Ewa, Franczyk-Zarow, Magdalena, Kostogrys, Renata, Dybas, Jakub, and Marzec, Katarzyna Maria
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2,3-Diphosphoglycerate ,Male ,Mice, Knockout ,Erythrocytes ,Atomic and Molecular Physics, and Optics ,Analytical Chemistry ,Mice, Inbred C57BL ,Mice ,Apolipoproteins E ,Spectroscopy, Fourier Transform Infrared ,Animals ,Female ,Instrumentation ,Spectroscopy - Abstract
VoR of manuscript of publication Spectrochim Acta A Mol Biomol Spectrosc. 2023;284:121819, Secondary structure alterations of RBC assessed by FTIR-ATR in correlation to 2,3-DPG levels in ApoE/LDLR–/– Mice (DOI: 10.1016/j.saa.2022.121819).
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- 2023
- Full Text
- View/download PDF
32. Click chemistry inspired synthesis of andrographolide triazolyl conjugates for effective fluorescent sensing of ferric ions
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Nishant, Pandey, Jyoti, Mangat, Singh, Pratibha, Dwivedi, Subash C, Sahoo, and Bhuwan B, Mishra
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Ions ,Azides ,Alkynes ,Iron ,Spectroscopy, Fourier Transform Infrared ,Organic Chemistry ,Click Chemistry ,Plant Science ,Biochemistry ,Copper ,Catalysis ,Analytical Chemistry - Abstract
The naturally occurring compound andrographolide 1 was used as a substrate for the synthesis of a novel terminal alkyne 3 which on copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction with azides 4a-l, 7 and 9 furnished a series of regioselective andrographolide triazolyl conjugates 5a-l, 8 and 10, respectively. A free glycoconjugate 6 was also prepared by selective deprotection of compound 5i in good yield. All the compounds were characterized by absorbance, FT-IR, NMR, and HR-MS analyses. The triazolyl conjugate 8 was further investigated as a probe for selective detection of Fe3+ ion in matrix. The mode of attachment of Fe3+ ion to the compound 8 was established by absorbance, fluorescence, infrared (IR), and nuclear magnetic resonance (NMR) spectroscopy, and high resolution mass-spectrometry (HR-MS). The logic gate circuits were constructed for the probe 8 and ethylenediaminetetraacetic acid (EDTA). The environmental perspective of probe 8 was investigated in real water samples.
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- 2021
33. Identification of AD‐18, 5F‐MDA‐19, and pentyl MDA‐19 in seized materials after the class‐wide ban of synthetic cannabinoids in China
- Author
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Tao Li, Wei Jia, Zhendong Hua, and Cuimei Liu
- Subjects
Cannabinoid receptor ,Cannabinoids ,Illicit Drugs ,Chemistry ,medicine.medical_treatment ,Pharmaceutical Science ,Nuclear magnetic resonance spectroscopy ,Mass spectrometry ,Gas Chromatography-Mass Spectrometry ,Mass Spectrometry ,Analytical Chemistry ,chemistry.chemical_compound ,Spectroscopy, Fourier Transform Infrared ,Synthetic cannabinoids ,medicine ,Cannabinoid receptor type 2 ,Environmental Chemistry ,Organic chemistry ,Cannabinoid ,Methylene ,Spectroscopy ,medicine.drug - Abstract
To curb the manufacturing, trafficking and abuse of synthetic cannabinoids, China implemented a class-wide regulation on synthetic cannabinoids in July 2021. Recently, three different types of synthetic cannabinoid analogs that were not covered by the generic definitions were detected in seized powdered and e-liquid materials. These derivatives included 2-(2-(1-(4-fluorobenzyl)-1H-indol-3-yl)acetamido)-3,3-dimethylbutanamide (AD-18), N'-(1-(5-fluoropentyl)-2-oxoindolin-3-ylidene)benzohydrazide (5F-MDA-19), and N'-(2-oxo-1-pentylindolin-3-ylidene)benzohydrazide (pentyl MDA-19). Identification was based on ultra-high-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry (UHPLC-QTOF-MS), gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance spectroscopy (NMR), and Fourier transform infrared spectroscopy (FT-IR). AD-18 is a methylene analog of ADB-FUBICA. No chemical or pharmacological data about AD-18 and 5F-MDA-19 have appeared until now, making this the first report on these two compounds. Pentyl MDA-19 has previously been reported to have high affinity for cannabinoid CB1 and CB2 receptors, but this is the first report of its presence in the recreational drug market. Moreover, the collision-induced dissociation (CID) and electron ionization (EI) characteristic fragmentation routes of AD-18 and the other two MDA-19 derivatives were also discussed to facilitate forensic laboratories in their identification of other substances with a similar structure in their case work.
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- 2021
34. Studies on the Vitrified and Cryomilled Bosentan
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Kinga Hyla, K. Koperwas, Krzysztof Chmiel, Rafał Łunio, Karolina Jurkiewicz, Daniel Żakowiecki, Marian Paluch, Aldona Minecka, Ewa Kamińska, Bartłomiej Milanowski, Barbara Hachuła, and Kamil Kaminski
- Subjects
Materials science ,Analytical chemistry ,Pharmaceutical Science ,Dielectric ,Article ,Differential scanning calorimetry ,X-Ray Diffraction ,Spectroscopy, Fourier Transform Infrared ,Drug Discovery ,cryomilling ,Fourier transform infrared spectroscopy ,dissolution rate ,Supercooling ,Dissolution ,molecular mobility ,Calorimetry, Differential Scanning ,bosentan ,vitrification ,Amorphous solid ,Dielectric spectroscopy ,Thermogravimetry ,Drug Liberation ,Dielectric Spectroscopy ,Molecular Medicine ,water removal - Abstract
In this paper, several experimental techniques [X-ray diffraction, differential scanning calorimetry (DSC), thermogravimetry, Fourier transform infrared spectroscopy, and broad-band dielectric spectroscopy] have been applied to characterize the structural and thermal properties, H-bonding pattern, and molecular dynamics of amorphous bosentan (BOS) obtained by vitrification and cryomilling of the monohydrate crystalline form of this drug. Samples prepared by these two methods were found to be similar with regard to their internal structure, H-bonding scheme, and structural (α) dynamics in the supercooled liquid state. However, based on the analysis of α-relaxation times (dielectric measurements) predicted for temperatures below the glass-transition temperature (Tg), as well as DSC thermograms, it was concluded that the cryoground sample is more aged (and probably more physically stable) compared to the vitrified one. Interestingly, such differences in physical properties turned out to be reflected in the lower intrinsic dissolution rate of BOS obtained by cryomilling (in the first 15 min of dissolution test) in comparison to the vitrified drug. Furthermore, we showed that cryogrinding of the crystalline BOS monohydrate leads to the formation of a nearly anhydrous amorphous sample. This finding, different from that reported by Megarry et al. [Carbohydr. Res.2011, 346, 1061−106421492830] for trehalose (TRE), was revealed on the basis of infrared and thermal measurements. Finally, two various hypotheses explaining water removal upon cryomilling have been discussed in the manuscript.
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- 2021
35. μATR-FTIR Spectral Libraries of Plastic Particles (FLOPP and FLOPP-e) for the Analysis of Microplastics
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Chelsea M. Rochman, Ronald Rubinovitz, and Hannah De Frond
- Subjects
chemistry.chemical_classification ,Microplastics ,Fourier Analysis ,Chemistry ,Analytical chemistry ,Polymer ,Spectral line ,Analytical Chemistry ,Attenuated total reflection ,Spectroscopy, Fourier Transform Infrared ,Principal component analysis ,Particle ,Fourier transform infrared spectroscopy ,Spectroscopy ,Plastics ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Raman spectral libraries specific to microplastics demonstrated improved spectral matching results when weathered plastics and a variety of particle colors and morphologies were included. Here, we explore if this is true for Fourier transform infrared (FTIR) spectroscopy as well. We present two novel databases specific to microplastics using attenuated total reflection (μATR-FTIR): (1) an FTIR library of plastic particles (FLOPP), containing 186 spectra from common plastic items, across 14 polymer types and (2) an FTIR library of plastic particles sourced from the environment (FLOPP-e), containing 195 spectra across 15 polymer types. Both libraries include particles from a variety of sources, morphologies, and colors. We demonstrate the applicability of these libraries for microplastics research by comparing spectral match results from two microplastic datasets. For this, we use different combinations of libraries including: commercially available reference libraries, an open-access polymer library, and FLOPP and FLOPP-e. Among tests, the greatest mean HQI result was achieved when the greatest number of libraries was included. This work demonstrates that spectral libraries specific to plastic particles found in the environment improve the accuracy of spectral matching and are best used in combination with commercial libraries containing chemical components that are commonly found within plastics and other anthropogenic particles. Multivariate principal component analyses of FLOPP and FLOPP-e spectra confirmed differences among polymer types and higher variation in principal component scores among weathered particles, but no patterns were observed among particle colors or morphologies. These results demonstrate that ATR-FTIR analyses are sensitive to weathering of plastics but not to particle color and morphology.
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- 2021
36. Deep Learning for Reconstructing Low-Quality FTIR and Raman Spectra─A Case Study in Microplastic Analyses
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Karin Mattsson, Martin Hassellöv, and Josef Brandt
- Subjects
Fourier Analysis ,Artificial neural network ,Noise (signal processing) ,business.industry ,Chemistry ,Microplastics ,Deep learning ,Pattern recognition ,Article ,Spectral line ,Analytical Chemistry ,symbols.namesake ,Deep Learning ,Quality (physics) ,Fourier transform ,Spectroscopy, Fourier Transform Infrared ,symbols ,Artificial intelligence ,Fourier transform infrared spectroscopy ,Raman spectroscopy ,business ,Plastics - Abstract
Herein we report on a deep-learning method for the removal of instrumental noise and unwanted spectral artifacts in Fourier transform infrared (FTIR) or Raman spectra, especially in automated applications in which a large number of spectra have to be acquired within limited time. Automated batch workflows allowing only a few seconds per measurement, without the possibility of manually optimizing measurement parameters, often result in challenging and heterogeneous datasets. A prominent example of this problem is the automated spectroscopic measurement of particles in environmental samples regarding their content of microplastic (MP) particles. Effective spectral identification is hampered by low signal-to-noise ratios and baseline artifacts as, again, spectral post-processing and analysis must be performed in automated measurements, without adjusting specific parameters for each spectrum. We demonstrate the application of a simple autoencoding neural net for reconstruction of complex spectral distortions, such as high levels of noise, baseline bending, interferences, or distorted bands. Once trained on appropriate data, the network is able to remove all unwanted artifacts in a single pass without the need for tuning spectra-specific parameters and with high computational efficiency. Thus, it offers great potential for monitoring applications with a large number of spectra and limited analysis time with availability of representative data from already completed experiments.
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- 2021
37. AFM investigation of APAC (antiplatelet and anticoagulant heparin proteoglycan)
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Riitta Lassila, Laurin Lux, Georg Ramer, Gernot Friedbacher, Bernhard Lendl, Annukka Jouppila, Maximilian Winzely, Karina Barreiro, Clinicum, Research Program in Systems Oncology, Institute for Molecular Medicine Finland, HUS Comprehensive Cancer Center, and Hematologian yksikkö
- Subjects
VON-WILLEBRAND-FACTOR ,medicine.drug_class ,Pharmacology ,Microscopy, Atomic Force ,Heparin proteoglycan ,Biochemistry ,FORCE ,Analytical Chemistry ,Atomic force microscopy ,Von Willebrand factor ,In vivo ,Spectroscopy, Fourier Transform Infrared ,BINDING ,Antithrombotic ,AFM-IR ,medicine ,Humans ,Antiplatelet ,Platelet ,Paper in Forefront ,SPECTROSCOPY ,biology ,Heparin ,Chemistry ,Anticoagulant ,Anticoagulants ,APAC ,DNA ,Photothermal-induced resonance ,Coagulation ,MICA ,biology.protein ,1182 Biochemistry, cell and molecular biology ,Proteoglycans ,3111 Biomedicine ,AFM ,Platelet Aggregation Inhibitors - Abstract
Antiplatelet and anticoagulant drugs are classified antithrombotic agents with the purpose to reduce blood clot formation. For a successful treatment of many known complex cardiovascular diseases driven by platelet and/or coagulation activity, the need of more than one antithrombotic agent is inevitable. However, combining drugs with different mechanisms of action enhances risk of bleeding. Dual anticoagulant and antiplatelet (APAC), a novel semisynthetic antithrombotic molecule, provides both anticoagulant and antiplatelet properties in preclinical studies. APAC is entering clinical studies with this new exciting approach to manage cardiovascular diseases. For a better understanding of the biological function of APAC, comprehensive knowledge of its structure is essential. In this study, atomic force microscopy (AFM) was used to characterize APAC according to its structure and to investigate the molecular interaction of APAC with von Willebrand factor (VWF), since specific binding of APAC to VWF could reduce platelet accumulation at vascular injury sites. By the optimization of drop-casting experiments, we were able to determine the volume of an individual APAC molecule at around 600 nm3, and confirm that APAC forms multimers, especially dimers and trimers under the experimental conditions. By studying the drop-casting behavior of APAC and VWF individually, we depictured their interaction by using an indirect approach. Moreover, in vitro and in vivo conducted experiments in pigs supported the AFM results further. Finally, the successful adsorption of APAC to a flat gold surface was confirmed by using photothermal-induced resonance, whereby attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) served as a reference method. Graphical abstract
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- 2021
38. Structure Elucidation Using Gas Chromatography−Infrared Spectroscopy/Mass Spectrometry Supported by Quantum Chemical IR Spectrum Simulations
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Philip J. Marriott, J. Shezmin Zavahir, Sandra Steiner, Susanne Salzmann, Chadin Kulsing, Yada Nolvachai, and Reinhard Dr Doetzer
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Quantum chemical ,Spectrophotometry, Infrared ,Molecular model ,Chemistry ,010401 analytical chemistry ,Analytical chemistry ,Infrared spectroscopy ,010402 general chemistry ,Mass spectrometry ,01 natural sciences ,Gas Chromatography-Mass Spectrometry ,Mass Spectrometry ,0104 chemical sciences ,Analytical Chemistry ,chemistry.chemical_compound ,Isomerism ,Spectroscopy, Fourier Transform Infrared ,Functional group ,Humans ,Gas chromatography ,Fourier transform infrared spectroscopy ,Basis set - Abstract
An improved strategy for compound identification incorporating gas chromatography hyphenated with Fourier transform infrared spectroscopy and mass spectroscopy (GC-FTIR/MS) is reported. (Over)reliance on MS may lead either to ambiguous identity or to incorrect identification of a compound. However, the MS result is useful to provide a cohort of possible compounds. The IR result for each tentative compound match was then simulated using molecular modeling, to provide functional group and isomer differentiation information, and then compared with the experimental FTIR result, offering identification based on both MS and IR. Several basis sets were evaluated for IR simulations; Def2-TZVPP was a suitable basis set and correlated well with experimental data. The approach was applied to industrial applications, confirming the isomers of 2,3-bis(thiosulfanyl)-but-2-enedinitrile, bromination products of 1-bromo-2,3-dimethylbenzene, and autoxidative degradation of phenyl-di-tert-butylphosphine.
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- 2021
39. Investigation of the performance of cross-linked poly(acrylic acid) and poly(methacrylic acid) as efficient adsorbents in SPE columns for simultaneous preconcentration of tricyclic antidepressants in water samples
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César Ricardo Teixeira Tarley, Felipe Augusto Gorla, Fernanda Midori de Oliveira, Clésia Cristina Nascentes, Milena do Prado Ferreira, Marcello Ferreira da Costa, and Mariana Gava Segatelli
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General Chemical Engineering ,Spectroscopy, Fourier Transform Infrared ,Solid Phase Extraction ,General Engineering ,Acrylic Resins ,Water ,Nortriptyline ,Antidepressive Agents, Tricyclic ,Analytical Chemistry - Abstract
A solid phase extraction-based (SPE) procedure for simultaneous preconcentration of five tricyclic antidepressants (TCAs), amitriptyline hydrochloride (AMT), nortriptyline hydrochloride (NOR), doxepin hydrochloride (DOX), imipramine hydrochloride (IMI), and clomipramine hydrochloride (CLO) from water samples with determination by HPLC-DAD is proposed. Polymers were characterized by FT-IR, SEM, and thermogravimetric analysis. SPE-based methods were carried out by the preconcentration of 320.0 mL of TCAs at pH 7.0 (buffered with 0.01 mol L
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- 2022
40. Evaluating Subtle Pathological Changes in Early Myocardial Ischemia Using Spectral Histopathology
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Tian Tian, Jianhua Zhang, Ling Xiong, Haixing Yu, Kaifei Deng, Xinbiao Liao, Fu Zhang, Ping Huang, Ji Zhang, and Yijiu Chen
- Subjects
Spectroscopy, Fourier Transform Infrared ,Myocardial Ischemia ,Myocardial Infarction ,Humans ,Proteins ,Pilot Projects ,Least-Squares Analysis ,Analytical Chemistry - Abstract
Early myocardial ischemia (EMI) is morphologically challenging, and the results from conventional histological staining may be subjective, imprecise, or even silent. The size of myocardial necrosis determines the acute and long-term mortality of EMI. The precise diagnosis of myocardial ischemia is critical for both clinical management and forensic investigation. Fourier transform infrared (FTIR) spectroscopic imaging is a highly sensitive tool for detecting protein conformations and imaging protein profiles. The aim of this study was to evaluate the application of FTIR imaging with multivariate analysis to detect biochemical changes in the protein conformation in the early phase of myocardial ischemia and to visually classify different disease states. The spectra and curve fitting results revealed that the total protein content decreased significantly in the EMI group and that the α-helix content of the secondary protein structure continuously decreased as ischemia progressed, while the β-sheet content increased. Differences in the control and EMI groups and perfused and ischemic myocardium were confirmed using principal component analysis and partial least squares discriminant analysis. Next, two support vector machine classifiers were effectively created. The accuracy, recall, and precision were 99.98, 99.96, and 100.00%, respectively, to differentiate the EMI group from the control group and 99.25, 98.95, and 99.54%, respectively, to differentiate perfused and ischemic myocardium. Ultimately, high EMI diagnostic accuracy was achieved with 100.00% recall and 100.00% precision, and ischemic myocardium diagnostic accuracy was achieved with 99.30% recall and 99.53% precision for the test set. This pilot study demonstrated that FTIR imaging is a powerful automated quantitative analysis tool to detect EMI without morphological changes and will improve diagnostic accuracy and patient prognosis.
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- 2022
41. On-Line Thermally Induced Evolved Gas Analysis: An Update-Part 2: EGA-FTIR
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Giuseppina Gullifa, Laura Barone, Elena Papa, Stefano Materazzi, and Roberta Risoluti
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Chemistry (miscellaneous) ,Organic Chemistry ,Drug Discovery ,Spectroscopy, Fourier Transform Infrared ,Molecular Medicine ,Pharmaceutical Science ,Physical and Theoretical Chemistry ,Analytical Chemistry - Abstract
The on-line thermally induced evolved gas analysis (OLTI-EGA) is widely applied in many different fields. Aimed to update the applications, our group has systematically collected and published examples of EGA characterizations. Following the recently published review on EGA-MS applications, this second part reviews the latest applications of Evolved Gas Analysis performed by on-line coupling heating devices to infrared spectrometers (EGA-FTIR). The selected 2019, 2020, 2021 and early 2022 references are collected and briefly described in this review; these are useful to help researchers to easily find applications that are sometimes difficult to locate.
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- 2022
42. Synthesis of hierarchical porous zirconium dioxide and its application in the detection of sulfonamides in animal-derived food
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Xiaofang Luan, Wen Nie, Xinxin Tian, Jinglei Xu, Wenqiang Fang, Shuang Liu, Xinyu Lan, Wenxuan Jia, Yongming Liu, and Zhenbo Liu
- Subjects
Sulfonamides ,Sulfanilamide ,General Chemical Engineering ,Spectroscopy, Fourier Transform Infrared ,General Engineering ,Animals ,Porosity ,Lipids ,Analytical Chemistry ,Polyethylene Glycols - Abstract
In order to effectively remove grease for the detection of sulfonamides, a non-toxic and low-cost hierarchical porous zirconia material was synthesized using the dual template method. The lipid impurities in an animal-derived food matrix can be absorbed by hierarchical zirconia. A ZrO
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- 2022
43. Biocomposites of Epoxidized Natural Rubber Modified with Natural Substances
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Konrad Stefaniak, Anna Masek, and Aleksandra Jastrzębska
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Polyesters ,Organic Chemistry ,Pharmaceutical Science ,Flavones ,Catechin ,Analytical Chemistry ,Chemistry (miscellaneous) ,ENR ,natural substances ,catechin hydrate ,flavone ,solar aging ,Spectroscopy, Fourier Transform Infrared ,Drug Discovery ,Epoxy Compounds ,Molecular Medicine ,Rubber ,Physical and Theoretical Chemistry - Abstract
This research aimed to show the possible impact of natural antioxidants on epoxidized natural rubber (ENR) and poly(lactic acid) (PLA) green composites. Thus, the ENR/PLA blends were prepared with the addition of three selected phytochemicals (catechin hydrate, eugenol and flavone). Obtained materials were submitted for solar aging. The analysis of the samples’ features revealed that catechin hydrate is a natural substance that may delay the degradation of ENR/PLA blends under the abovementioned conditions. The blend loaded with catechin hydrate presented stable color parameters (dE < 3 a.u.), the highest aging coefficient (K = 0.38 a.u.) and the lowest carbonyl index based on FT-IR data (CI = 1.56) from among all specimens. What is more, this specimen prolonged the oxidation induction time in comparison with the reference samples. Gathered data prove the efficiency of catechin hydrate as an anti-aging additive. Additionally, it was found that a specimen loaded with flavone changed its color parameters significantly after solar aging (dE = 14.83 a.u.) so that it would be used as an aging indicator. Eventually, presented eco-friendly ENR-based compositions may be applied in polymer technology where materials presenting specific properties are desirable.
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- 2022
- Full Text
- View/download PDF
44. Comparative Study of Antimicrobial Activity of Silver, Gold, and Silver/Gold Bimetallic Nanoparticles Synthesized by Green Approach
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Naila Sher, Dalal Hussien M. Alkhalifah, Mushtaq Ahmed, Nadia Mushtaq, Faridullah Shah, Fozia Fozia, Rahmat Ali Khan, Wael N. Hozzein, and Mourad A. M. Aboul-Soud
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Methicillin-Resistant Staphylococcus aureus ,Silver ,Plant Extracts ,Organic Chemistry ,Hippeastrum hybridum ,nanotechnology ,NPs synthesis ,antimicrobial activities ,Metal Nanoparticles ,Pharmaceutical Science ,Pneumonia ,Anti-Bacterial Agents ,Analytical Chemistry ,Anti-Infective Agents ,Chemistry (miscellaneous) ,Spectroscopy, Fourier Transform Infrared ,Drug Discovery ,Escherichia coli ,Humans ,Molecular Medicine ,Gold ,Physical and Theoretical Chemistry - Abstract
Nanotechnology is one of the most recent technologies. It is uncertain whether the production of small-size nanoparticles (NPs) can be achieved through a simple, straightforward, and medicinally active phytochemical route. The present study aimed to develop an easy and justifiable method for the synthesis of Ag, Au, and their Ag/Au bimetallic NPs (BNPs) by using Hippeastrum hybridum (HH) extract, and then to investigate the effects of Ag, Au, and their Ag/Au BNPs as antimicrobial and phytotoxic agents. Ag, Au, and their Ag/Au BNPs were characterized by UV-visible spectroscopy, FT-IR spectroscopy, XRD, EDX, and SEM analysis. XRD analysis conferring to the face of face-centered cubic crystal structure with an average size of 13.3, 10.72, and 8.34 nm of Ag, Au, and Ag/Au BNPs, respectively. SEM showed that Ag, Au, and Ag/Au BNPs had spherical morphologies, with calculated nano measurements of 40, 30, and 20 nm, respectively. The EDX analysis confirmed the composition of elemental Ag signal of the HH-AgNPs with 22.75%, Au signal of the HH-AuNPs with 48.08%, Ag signal with 12%, and Au signal with 38.26% of the Ag/Au BNPs. The Ag/Au BNPs showed an excellent antimicrobial efficacy against Gram-positive Staphylococcus aureus, Actinomycetes meriye, Bacillus cereus, Streptococcus pyogenes, Methicillin-resistant Staphylococcus aureus, Micrococcus luteus, Streptococcus pneumonia, and Gram-negative Klebsiella pneumonia, Escherichia coli, and Serratia marcescens bacterial strains, as well as against three fungal strains (Aspergillus niger, Aspergillus fumigatus, and Aspergillus flavus) compared to HH extract, HH-AgNPs, and HH-AuNPs. However, further investigations are recommended to be able to minimize potential risks of application.
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- 2022
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45. From Materials to Technique: A Complete Non-Invasive Investigation of a Group of Six Ukiyo-E Japanese Woodblock Prints of the Oriental Art Museum E. Chiossone (Genoa, Italy)
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Marco Gargano, Margherita Longoni, Valeria Pesce, Maria Chiara Palandri, Aurora Canepari, Nicola Ludwig, and Silvia Bruni
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Japan ,Italy ,Museums ,Spectroscopy, Fourier Transform Infrared ,Fiber Optic Technology ,Electrical and Electronic Engineering ,Japanese woodblock print ,multiband imaging ,UV fluorescence ,visible induced luminescence ,reflectance transformation imaging ,Raman spectroscopy ,reflectance FTIR spectroscopy ,spectrofluorimetry ,fiber optic reflectance spectroscopy ,Biochemistry ,Instrumentation ,Atomic and Molecular Physics, and Optics ,Analytical Chemistry - Abstract
In the present work, a complete non-invasive scientific investigation of six Utagawa Kunisada’s woodblock prints (nishiki-e) belonging to the Oriental Art Museum “E. Chiossone” (Genoa, Italy), was performed in situ. The campaign started with high resolution multiband imaging (visible, multiband fluorescence, near infrared) followed by reflectance transformation imaging (RTI) to characterize and highlight the peculiar printing techniques and the condition of the support. Then fiber optics reflectance spectroscopy (FORS), spectrofluorimetry, Raman and reflectance Fourier-transform infrared (FTIR) spectroscopies were successfully applied in synergy for the investigation of the printing materials (pigments, binders, support). The results obtained represent a set of very important information for these never-before-studied works of art, useful to the different professionals involved: historians, conservators and curators. The materials identified were completely in agreement with those traditionally used in the Edo period in the 19th century, while the computational imaging technique RTI gave an additional amount of information in terms of surface characterization that could not be overlooked when studying these works of art. RTI data were further processed to enhance the texture visualization.
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- 2022
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46. Visual and Physical Degradation of the Black and White Mosaic of a Roman Domus under Palazzo Valentini in Rome: A Preliminary Study
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Claudia Colantonio, Paola Baldassarri, Pasquale Avino, Maria Luisa Astolfi, and Giovanni Visco
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Magnetic Resonance Spectroscopy ,Organic Chemistry ,Rome ,Pharmaceutical Science ,FT-IR ,GC-MS ,NMR ,Roman domus ,mosaic restoration ,multi-method diagnostic ,Gas Chromatography-Mass Spectrometry ,Analytical Chemistry ,Archaeology ,Chemistry (miscellaneous) ,Spectroscopy, Fourier Transform Infrared ,Fourier Transform Infrared ,Drug Discovery ,Molecular Medicine ,Physical and Theoretical Chemistry ,Spectroscopy - Abstract
Palazzo Valentini, the institutional head office of Città Metropolitana di Roma Capitale, stands in in a crucial position in the Roman archaeological and urban contexts, exactly between the Fora valley, Quirinal Hill slopes, and Campus Martius. It stands on a second-century A.D. complex to which belong, between other archeological remains, two richly decorated aristocratic domus. One of these buildings, the domus A, presents an outward porticoed room with a fourth-century AD central impluvium (open air part of the atrium designed to carry away rainwater) with a black/white tiled mosaic pavement, the preservation status of which is compromised by an incoherent degradation product that has caused gradual detachment of the mosaic tiles. To identify the product and determine the causes of degradation, samples of the product were taken and subjected to SEM-EDS, XRF, NMR, FT-IR and GC-MS analyses. The findings reported in this study can help restorers, archaeologists and conservation scientists in order to improve knowledge about the Roman mosaic, its construction phases, conservation problems and proper solutions.
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- 2022
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47. Insights into the Inhibitory Mechanism of Viniferifuran on Xanthine Oxidase by Multiple Spectroscopic Techniques and Molecular Docking
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Yaxin, Yang, Qian, Chen, Shiyang, Ruan, Junli, Ao, and Shang-Gao, Liao
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Molecular Docking Simulation ,Xanthine Oxidase ,Kinetics ,Chemistry (miscellaneous) ,Spectroscopy, Fourier Transform Infrared ,Organic Chemistry ,Drug Discovery ,Molecular Medicine ,Pharmaceutical Science ,viniferifuran ,xanthine oxidase ,inhibition mechanism ,Enzyme Inhibitors ,Physical and Theoretical Chemistry ,Analytical Chemistry - Abstract
Viniferifuran was investigated for its potential to inhibit the activity of xanthine oxidase (XO), a key enzyme catalyzing xanthine to uric acid. An enzyme kinetics analysis showed that viniferifuran possessed a strong inhibition on XO in a typical anti-competitive manner with an IC50 value of 12.32 μM (IC50 for the first-line clinical drug allopurinol: 29.72 μM). FT-IR and CD data analyses showed that viniferifuran could induce a conformational change of XO with a decrease in the α-helix and increases in the β-sheet, β-turn, and random coil structures. A molecular docking analysis revealed that viniferifuran bound to the amino acid residues located within the activity cavity of XO by a strong hydrophobic interaction (for Ser1214, Val1011, Phe914, Phe1009, Leu1014, and Phe649) and hydrogen bonding (for Asn768, Ser876, and Tyr735). These findings suggested that viniferifuran might be a promising XO inhibitor with a favorable mechanism of action.
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- 2022
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48. [Determination of four fungicides in water by magnetic solid phase extraction-ultrahigh performance liquid chromatography-tandem mass spectrometry using covalent organic framework material]
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Pan WANG, Jiping MA, Shuang LI, Jiawen CHENG, and Zongyue ZOU
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General Chemical Engineering ,Magnetic Phenomena ,Organic Chemistry ,Solid Phase Extraction ,Water ,Hydrogen-Ion Concentration ,Biochemistry ,Analytical Chemistry ,Fungicides, Industrial ,Soil ,Tandem Mass Spectrometry ,Spectroscopy, Fourier Transform Infrared ,Electrochemistry ,Humans ,Chromatography, High Pressure Liquid ,Metal-Organic Frameworks - Abstract
Fungicides can lead to soil and plant diseases after long-term enrichment in the environment; they can also penetrate deeper into the soil and groundwater by rainwater or irrigation, threatening the water environment and human health. Therefore, it is crucial to develop a simple, rapid, efficient, and sensitive analytical method for the detection of fungicides in the water environment. Sample pretreatment is important for the extraction and enrichment of pollutants from environmental water. Magnetic solid phase extraction (MSPE) is a new sample pretreatment method, which uses magnetic materials as adsorbents dispersed in solution, and rapid separation can be achieved by the aid of external magnets. Because of its advantages of short analytical time, less organic solvent consumption, and easy separation of adsorbents, MSPE has attracted much attention. The key to MSPE is the preparation of highly selective magnetic adsorbents. Covalent organic frameworks have the advantages of large surface area, good chemical and thermal stability, tunable porous structure, low density, and easy functionalization, all of which are ideal for adsorbing fungicides. The concentration of fungicides in environmental water is low. Ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) has high sensitivity and high selectivity, which is suitable for the analysis of fungicides. In this work, a magnetic covalent organic framework Fe
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- 2022
49. One-Step Synthesis of Aminobenzoic Acid Functionalized Graphene Oxide by Electrochemical Exfoliation of Graphite for Oxygen Reduction to Hydrogen Peroxide and Supercapacitors
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Yuting Lei, Ludmila dos Santos Madalena, Benjamin D. Ossonon, Fausto Eduardo Bimbi Junior, Jiyun Chen, Marcos R. V. Lanza, and Ana C. Tavares
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Oxygen ,Chemistry (miscellaneous) ,electrochemical exfoliation of graphite ,amine oxidation ,graphene oxide ,aminobenzoic acid ,oxygen reduction reaction ,supercapacitors ,Spectroscopy, Fourier Transform Infrared ,Organic Chemistry ,Drug Discovery ,Molecular Medicine ,Pharmaceutical Science ,Graphite ,Hydrogen Peroxide ,Physical and Theoretical Chemistry ,4-Aminobenzoic Acid ,Analytical Chemistry - Abstract
Graphene-based materials have attracted considerable attention as promising electrocatalysts for the oxygen reduction reaction (ORR) and as electrode materials for supercapacitors. In this work, electrochemical exfoliation of graphite in the presence of 4-aminebenzoic acid (4-ABA) is used as a one-step method to prepare graphene oxide materials (EGO) functionalized with aminobenzoic acid (EGO-ABA). The EGO and EGO-ABAs materials were characterized by FT-IR spectroscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, X-ray diffraction and scanning electron microscopy. It was found that the EGO-ABA materials have smaller flake size and higher density of oxygenated functional groups compared to bare EGO. The electrochemical studies showed that the EGO-ABA catalysts have higher activity for the ORR to H2O2 in alkaline medium compared to EGO due to their higher density of oxygenated functional groups. However, bare EGO has a higher selectivity for the 2-electron process (81%) compared to the EGO-ABA (between 64 and 72%) which was related to a lower content of carbonyl groups. The specific capacitance of the EGO-ABA materials was higher than that of EGO, with an increase by a factor of 3 for the materials prepared from exfoliation in 5 mM 4-ABA/0.1 M H2SO4. This electrode material also showed a remarkable cycling capability with a loss of only 19.4% after 5000 cycles at 50 mVs−1.
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- 2022
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50. Lysinated Multiwalled Carbon Nanotubes with Carbohydrate Ligands as an Effective Nanocarrier for Targeted Doxorubicin Delivery to Breast Cancer Cells
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Chanchal Kiran Thakur, Rabin Neupane, Chandrabose Karthikeyan, Charles R. Ashby, R. Jayachandra Babu, Sai H. S. Boddu, Amit K. Tiwari, and Narayana Subbiah Hari Narayana Moorthy
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Nanotubes, Carbon ,Organic Chemistry ,Pharmaceutical Science ,Breast Neoplasms ,Antineoplastic Agents ,Ligands ,Analytical Chemistry ,Drug Delivery Systems ,Doxorubicin ,Chemistry (miscellaneous) ,Spectroscopy, Fourier Transform Infrared ,Drug Discovery ,Tumor Microenvironment ,Humans ,Molecular Medicine ,Female ,Physical and Theoretical Chemistry ,drug delivery ,carbohydrate ,multiwalled carbon nanotubes ,lysinated MWCNTs ,breast cancer ,cellular uptake - Abstract
Multiwalled carbon nanotubes (MWCNTs) are elongated, hollow cylindrical nanotubes made of sp2 carbon. MWCNTs have attracted significant attention in the area of drug delivery due to their high drug-loading capacity and large surface area. Furthermore, they can be linked to bioactive ligands molecules via covalent and noncovalent bonds that allow for the targeted delivery of anticancer drugs such as doxorubicin. The majority of methodologies reported for the functionalization of MWCNTs for drug delivery are quite complex and use expensive linkers and ligands. In the present study, we report a simple, cost-effective approach for functionalizing MWCNTs with the carbohydrate ligands, galactose (GA), mannose (MA) and lactose (LA), using lysine as a linker. The doxorubicin (Dox)-loaded functionalized MWCNTs were characterized using FT-IR, NMR, Raman, XRD and FE-SEM. The drug–loaded MWCNTs were evaluated for drug loading, drug release and cell toxicity in vitro, in breast cancer cells. The results indicated that the carbohydrate-modified lysinated MWCNTs had greater Dox loading capacity, compared to carboxylated MWCNTs (COOHMWCNTs) and lysinated MWCNTs (LyMWCNTs). In vitro drug release experiments indicated that the carbohydrate functionalized LyMWCNTs had higher Dox release at pH 5.0, compared to the physiological pH of 7.4, over 120 h, indicating that they are suitable candidates for targeting the tumor microenvironment as a result of their sustained release profile of Dox. Doxorubicin-loaded galactosylated MWCNTs (Dox-GAMWCNTs) and doxorubicin loaded mannosylated MWCNTs (Dox-MAMWCNTs) had greater anticancer efficacy and cellular uptake, compared to doxorubicin–loaded lactosylated MWCNTs (Dox-LAMWCNTs) and pure Dox, in MDA-MB231 and MCF7 breast cancer cells. However, neither the ligand conjugated multiwall blank carbon nanotubes (GAMWCNTs, MAMWCNTs and LAMWCNTs) nor the lysinated multiwalled blank carbon nanotubes produced significant toxicity in the normal cells. Our results suggest that sugar-tethered multiwalled carbon nanotubes, especially the galactosylated (Dox-GAMWCNTs) and mannosylated (Dox-MAMWCNTs) formulations, may be used to improve the targeted delivery of anticancer drugs to breast cancer cells.
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- 2022
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