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Your search keyword '"Beck-Schimmer, Beatrice"' showing total 2 results
Start Over Author "Beck-Schimmer, Beatrice" Topic anatomy Topic cytokines
2 results on '"Beck-Schimmer, Beatrice"'

1. Modulation of early inflammatory response by different balanced and non-balanced colloids and crystalloids in a rodent model of endotoxemia

Author

Voigtsberger, Stefanie, Urner, Martin, Hasler, Melanie, Roth Z'graggen, Birgit, Booy, Christa, Spahn, Donat R, Beck-Schimmer, Beatrice, and University of Zurich

Subjects
Male, Critical Care and Emergency Medicine, Physiology, Chemokine CXCL1, lcsh:Medicine, Kidney, Pathology and Laboratory Medicine, 10052 Institute of Physiology, Anesthesiology, Immune Physiology, Medicine and Health Sciences, lcsh:Science, Lung, Immune Response, Chemokine CCL2, Mammals, Hematology, Animal Models, Intercellular Adhesion Molecule-1, Liver, Perioperative Hemostasis, 10076 Center for Integrative Human Physiology, Vertebrates, Cytokines, Anatomy, Research Article, 10216 Institute of Anesthesiology, Fluid Management, Immunology, 610 Medicine & health, 1100 General Agricultural and Biological Sciences, Research and Analysis Methods, Rodents, Signs and Symptoms, Model Organisms, 1300 General Biochemistry, Genetics and Molecular Biology, Sepsis, Animals, Colloids, RNA, Messenger, Rats, Wistar, Inflammation, 1000 Multidisciplinary, Renal Physiology, Hemostasis, Tumor Necrosis Factor-alpha, lcsh:R, Immunity, Organisms, Biology and Life Sciences, Crystalloid Solutions, Renal System, Molecular Development, Endotoxemia, Rats, Disease Models, Animal, 570 Life sciences, biology, lcsh:Q, Isotonic Solutions, Developmental Biology
Abstract

The use of hydroxyethyl starch (HES) in sepsis has been shown to increase mortality and acute kidney injury. However, the knowledge of the exact mechanism by which several fluids, especially starch preparations may impair end-organ function particularly in the kidney, is still missing. The aim of this study was to measure the influence of different crystalloid and colloid fluid compositions on the inflammatory response in the kidney, the liver and the lung using a rodent model of acute endotoxemia. Rats were anesthetized and mechanically ventilated. Lipopolysaccharide (5 mg/kg) was administered intravenously. After one hour crystalloids [lactate-buffered (RLac) or acetate-buffered (RAc)] were infused i.v. (30 ml/kg) in all groups. At 2 hours rats either received different crystalloids (75 ml/kg of RLac or RAc) or colloids (25 ml/kg of HES in saline or HES in RAc or gelatin in saline). Expression of messenger RNA for cytokine-induced neutrophil chemoattractant-1 (CINC-1), monocyte chemotactic protein-1 (MCP-1), necrosis factor α (TNFα) and intercellular adhesion molecule 1 (ICAM-1) was assessed in kidney, liver and lung tissue by real-time PCR after 4 hours. The use of acetate-buffered solutions was associated with a significantly higher expression of CINC-1 and TNFα mRNA in the liver, in the kidney and in the lung. Only marginal effects of gelatin and hydroxyethyl starch on mRNA expression of inflammatory mediators were observed. The study provides evidence that the type of buffering agent of different colloidal and crystalloid solutions might be a crucial factor determining the extent of early end-organ inflammatory response in sepsis.

Published
2014

2. Inflammatory Kidney and Liver Tissue Response to Different Hydroxyethylstarch (HES) Preparations in a Rat Model of Early Sepsis.

Author

Schimmer, Ralph C., Urner, Martin, Voigtsberger, Stefanie, Booy, Christa, Roth Z’Graggen, Birgit, Beck-Schimmer, Beatrice, and Schläpfer, Martin

Subjects
KIDNEY injuries, SEPSIS, LIVER physiology, LABORATORY rats, ENDOTHELIAL cells, IN vitro studies
Abstract

Background: Tissue hypoperfusion and inflammation in sepsis can lead to organ failure including kidney and liver. In sepsis, mortality of acute kidney injury increases by more than 50%. Which type of volume replacement should be used is still an ongoing debate. We investigated the effect of different volume strategies on inflammatory mediators in kidney and liver in an early sepsis model. Material and Methods: Adult male Wistar rats were subjected to sepsis by cecal ligation and puncture (CLP) and assigned to three fluid replenishment groups. Animals received 30mL/kg of Ringer’s lactate (RL) for 2h, thereafter RL (75mL/kg), hydroxyethyl starch (HES) balanced (25mL/kg), containing malate and acetate, or HES saline (25mL/kg) for another 2h. Kidney and liver tissue was assessed for inflammation. In vitro rat endothelial cells were exposed to RL, HES balanced or HES saline for 2h, followed by stimulation with tumor necrosis factor-α (TNF-α) for another 4h. Alternatively, cells were exposed to malate, acetate or a mixture of malate and acetate, reflecting the according concentration of these substances in HES balanced. Pro-inflammatory cytokines were determined in cell supernatants. Results: Cytokine mRNA in kidney and liver was increased in CLP animals treated with HES balanced compared to RL, but not after application of HES saline. MCP-1 was 3.5fold (95% CI: 1.3, 5.6) (p<0.01) and TNF-α 2.3fold (95% CI: 1.2, 3.3) (p<0.001) upregulated in the kidney. Corresponding results were seen in liver tissue. TNF-α-stimulated endothelial cells co-exposed to RL expressed 3529±1040pg/mL MCP-1 and 59±23pg/mL CINC-1 protein. These cytokines increased by 2358pg/mL (95% CI: 1511, 3204) (p<0.001) and 29pg/ml (95% CI: 14, 45) (p<0.01) respectively when exposed to HES balanced instead. However, no further upregulation was observed with HES saline. PBS supplemented with acetate increased MCP-1 by 1325pg/mL (95% CI: 741, 1909) (p<0.001) and CINC-1 by 24pg/mL (95% CI: 9, 38) (p<0.01) compared to RL. Malate as well as HES saline did not affect cytokine expression. Conclusion: We identified HES balanced and specifically its component acetate as pro-inflammatory factor. How important this additional inflammatory burden on kidney and liver function is contributing to the sepsis-associated inflammatory burden in early sepsis needs further evaluation. [ABSTRACT FROM AUTHOR]

Published
2016
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