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1. Contents, Vol. 145, 1992

Author

R.L. Richardson, B. Essén-Gustavsson, J.M. Riesco, S. Doehm, Ruiz Tomer, E. Stofft, H. Hoppeler, A. Rodriguez, S. Emura, J.A. Juanes, T. Naitoh, G. Bogusch, S. Shoumura, G.J. Hausman, B. Klaus, A. Tamada, A. Ribeiro, R. Straub, C.G. Brown, U. Hose, C.A. Brown, K. Yamashita, K. Karhtröm, K. Birnbaum, W. Breipohl, D. Hayakawa, T. Takagi, A. Abou-Elmagd, K. Romaniuk, A. Gocht, U.S. Amonoo-Kuofi, M. Weishaupt, Martínez Soriano, P. Cobos, J. Düllmann, F. Sánchez, R. Kujat, J. Carretero, R.L. Lieber, R.J. Wassersug, K.-H. Wrobel, P. Nielsen, U. Korn, A. Sander, Hernandez Gil de Tejada, A. Cobos, M. Rubio, T. Hertz, W. Lierse, Sanchez Herrador, D. Steiner, M. Arakawa, H. Chen, M. Utsumi, V. Piera, H.R. Gaskins, N.R. Grande, E. Carvalho, K. Terasawa, M.F. Neurath, J. Menck, H. Isono, De Sousa Pereira, T. Yamahira, E. Blanco, R. Vázquez, W. Young, M. Torrente, and O. de la Garza

Subjects
Histology, Anatomy
Published
1992

2. Subject Index, Vol. 145, 1992

Author

E. Carvalho, O. de la Garza, S. Doehm, B. Klaus, R. Vázquez, W. Young, U. Hose, T. Takagi, A. Abou-Elmagd, M. Rubio, R.L. Lieber, A. Cobos, K. Terasawa, R. Straub, K. Romaniuk, M. Utsumi, W. Breipohl, D. Hayakawa, De Sousa Pereira, R. Kujat, K. Yamashita, F. Sánchez, P. Cobos, M. Weishaupt, Martínez Soriano, W. Lierse, B. Essén-Gustavsson, A. Sander, T. Yamahira, H. Hoppeler, S. Shoumura, Hernandez Gil de Tejada, N.R. Grande, E. Blanco, M. Torrente, M.F. Neurath, M. Arakawa, H. Chen, V. Piera, T. Hertz, J.A. Juanes, J. Menck, J. Carretero, Sanchez Herrador, G. Bogusch, G.J. Hausman, H. Isono, A. Tamada, J. Düllmann, U. Korn, C.A. Brown, R.J. Wassersug, C.G. Brown, D. Steiner, K.-H. Wrobel, H.R. Gaskins, U.S. Amonoo-Kuofi, A. Gocht, P. Nielsen, R.L. Richardson, A. Rodriguez, A. Ribeiro, S. Emura, T. Naitoh, J.M. Riesco, Ruiz Tomer, E. Stofft, K. Karhtröm, and K. Birnbaum

Subjects
Histology, Index (economics), Statistics, Subject (documents), Anatomy, Mathematics
Published
1992

3. Adrenergic Innervation of Fetal Pig Adipose Tissue

Author

Gary J. Hausman and R.L. Richardson

Subjects
medicine.medical_specialty, Fetus, Histology, Hypophysectomy, business.industry, medicine.medical_treatment, Adipose tissue, Adrenergic, Endocrinology, Internal medicine, medicine, Ultrastructure, Immunohistochemistry, Subcutaneous adipose tissue, Anatomy, business, Fetal pig
Abstract

Histochemistry and electron microscopy were used to study the adrenergic innervation of subcutaneous adipose tissue in fetal pigs. Adrenergic innervation was present around arteries, arterioles, and adipocyte-associated capillaries. Nerve fibers were infrequently observed around veins, venules, and adipocytes. Ultrastructural features of nerves included mitochondria, clear synaptic vesicles, and a small number of vesicles with a core of electron-dense material. Innervation of adipose tissue was similar in 70-, 90-, and 110-day-old fetuses. Examination of fetuses decapitated at 45 days of gestation and removed at 110 days showed that adrenergic innervation was absent in adipose tissue of decapitated fetuses. Adrenergic innervation was also absent in adipose tissue from fetuses hypophysectomized at 72–73 days of gestation and examined at 110 days. These data indicate that fetal porcine adipose tissue may be under neural control by day 70 of gestation. Furthermore, an intact pituitary is necessary for the development of adrenergic innervation in fetal adipose tissue.

Published
1987

4. Adipocyte development in primary rat cell cultures: A scanning electron microscopy study

Author

R.L.. Richardson, D. R. Campion, Gary J. Hausman, and G. B. Thomas

Subjects
Stromal cell, Cell Survival, Adipose tissue, Biology, chemistry.chemical_compound, symbols.namesake, Adipocyte, Animals, Cells, Cultured, Cytoskeleton, Rats, Inbred Strains, Golgi apparatus, Lipid Metabolism, Agricultural and Biological Sciences (miscellaneous), Rats, Cell biology, Adipose Tissue, Biochemistry, chemistry, Osmium tetroxide, Cell culture, Microscopy, Electron, Scanning, symbols, Ultrastructure, Glutaraldehyde, Anatomy, Electron Probe Microanalysis
Abstract

This study utilized scanning electron microscopy (SEM) techniques to observe primary cultures of stromal-vascular (SV) cells derived from postnatal rat inguinal adipose tissue. Cells were grown on collagen-coated, fibronectin-coated, or uncoated glass coverslips. Coverslips were normally fixed in glutaraldehyde, osmium tetroxide, dehydrated, and critical-point-dried. Other coverslips were frozen in isopentane (cooled in LN2) and dried or fixed in Baker's formalin for demonstration of inosine diphosphatase (IDPase) by X-ray microprobe analysis (XRMA). Adipocyte morphologies were similar on all substrates. At 2 days of culture, actin cables were detected extending from developing adipocytes. No difference in actin cable structure, cellular shape, or lipid accumulation was observed among the different substrates. Some stromal cells did not accumulate lipid but proliferated into a multilayer by 9 days in culture. Inosine diphosphatase was detected in the Golgi apparatus of developing adipocytes utilizing the technique of XRMA. This study demonstrates the potential for using SEM and XRMA techniques to define morphological features and cytochemical markers of adipocytes in vitro and the response of primary cultured rat SV cells to other attachment substrates.

Published
1986

5. Subject Index, Vol. 130, 1987

Author

Michael J. Ulissey, A. Borregón, Klaus Fischer, P.E. Spoerri, J.A. Garcís-Porrero, Jack Davies, Elisabeth Vogel, G.J. Hausman, Christopher R. Murphy, J. More, Calvo Jl, A. Amara, Judy Lefkowitz, Michael E. Trulson, Margot J. Hosie, B. Rama, J. Sautet, J. Leeton, A.L. Carbonell, Peter Rogers, M.A. Ros, Stefan Nachbaur, J.L. Ojeda, Gabriele M. Rune, Johannes Lang, Tatsumi Nakano, L. Beaton, J. Boya, C.J. Catalina-Herrera, Christian Hack, Eduardo Couve, G. Van Haverbeke, R.L. Richardson, Anne M. Macpherson, Y. Ogawa, H. Muto, and P. Cabanie

Subjects
Histology, Index (economics), Statistics, Subject (documents), Anatomy, Mathematics
Published
1987

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