1. Linalool acts as a fast and reversible anesthetic in Hydra
- Author
-
Sara Martin, Elizabeth Lanphear, Rui Wang, Tapan Goel, and Eva-Maria S. Collins
- Subjects
Insecticides ,Fluorescence-lifetime imaging microscopy ,Hydra ,Urethanes ,Animals, Genetically Modified ,chemistry.chemical_compound ,0302 clinical medicine ,Linalool ,Anesthesiology ,Animal Cells ,Medicine and Health Sciences ,Morphogenesis ,Anesthesia ,Neurons ,0303 health sciences ,Multidisciplinary ,Pharmaceutics ,Chemistry ,Eukaryota ,Drugs ,Esters ,Animal Models ,Cell biology ,In Vivo Imaging ,Experimental Organism Systems ,Physical Sciences ,Medicine ,Lernaean Hydra ,Cellular Types ,Preclinical imaging ,Research Article ,medicine.drug ,Imaging Techniques ,Acyclic Monoterpenes ,Science ,Research and Analysis Methods ,Cnidaria ,03 medical and health sciences ,Drug Therapy ,In vivo ,Fluorescence Imaging ,medicine ,Animals ,Pain Management ,Regeneration ,Anesthetics ,030304 developmental biology ,Pharmacology ,Head Regeneration ,Regeneration (biology) ,Organisms ,Chemical Compounds ,Biology and Life Sciences ,Cell Biology ,Invertebrates ,Transplantation ,Cellular Neuroscience ,Anesthetic ,Animal Studies ,Organism Development ,Head ,030217 neurology & neurosurgery ,Developmental Biology ,Neuroscience - Abstract
The ability to make transgenic Hydra lines has allowed for quantitative in vivo studies of Hydra regeneration and physiology. These studies commonly include excision, grafting and transplantation experiments along with high-resolution imaging of live animals, which can be challenging due to the animal's response to touch and light stimuli. While various anesthetics have been used in Hydra studies, they tend to be toxic over the course of a few hours or their long-term effects on animal health are unknown. Here, we show that the monoterpenoid alcohol linalool is a useful anesthetic for Hydra. Linalool is easy to use, non-toxic, fast acting, and reversible. It has no detectable long-term effects on cell viability or cell proliferation. We demonstrate that the same animal can be immobilized in linalool multiple times at intervals of several hours for repeated imaging over 2-3 days. This uniquely allows for in vivo imaging of dynamic processes such as head regeneration. We directly compare linalool to currently used anesthetics and show its superior performance. Linalool will be a useful tool for tissue manipulation and imaging in Hydra research in both research and teaching contexts.
- Published
- 2019