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Your search keyword '"Kun Chen"' showing total 4 results
Start Over Author "Kun Chen" Topic angiogenesis Topic inflammation
4 results on '"Kun Chen"'

1. Intra-carotid arterial transfusion of circulatory-derived autologous endothelial progenitor cells in rodent after ischemic stroke-evaluating the impact of therapeutic time points on prognostic outcomes

Author

Kuan-Hung Chen, Pei-Lin Shao, Sheung-Fat Ko, Yi-Chen Li, Hon-Kan Yip, Pei-Hsun Sung, Han-Tan Chai, Kun-Chen Lin, John Y. Chiang, and Chi-Ruei Huang

Subjects
Male, medicine.medical_specialty, Angiogenesis, Medicine (miscellaneous), Inflammation, Rodentia, medicine.disease_cause, Biochemistry, Genetics and Molecular Biology (miscellaneous), Gastroenterology, Brain Ischemia, lcsh:Biochemistry, Rats, Sprague-Dawley, Neurological function, Internal medicine, Medicine, Animals, lcsh:QD415-436, Progenitor cell, Endothelial progenitor cells, lcsh:R5-920, Ischemic stroke, business.industry, Research, Cell Biology, Prognosis, Rats, Endothelial stem cell, Stroke, Apoptosis, Circulatory system, Molecular Medicine, Stem cell, medicine.symptom, business, lcsh:Medicine (General), Oxidative stress
Abstract

Background This study tested the optimal time point for left intra-carotid arterial (LICA) administration of circulatory-derived autologous endothelial progenitor cells (EPCs) for improving the outcome in rat after acute ischemic stroke (IS). Methods and results Adult male SD rats (n = 70) were equally categorized into group 1 (sham-operated control), group 2 (IS), group 3 (IS+EPCs/1.2 × 106 cells/by LICA administration 3 h after IS), group 4 (IS+EPCs/LICA administration post-day-3 IS), group 5 (IS+EPCs/LICA administration post-day-7 IS), group 6 (IS+EPCs/LICA administration post-day-14 IS), and group 7 (IS+EPCs/LICA administration post-day-28 IS). The brain infarct volume (BIV) (at day 60/MRI) was lowest in group 1, highest in group 2, and significantly progressively increased from groups 3 to 7, whereas among the IS animals, the neurological function was significantly preserved in groups 3 to 6 than in groups 2 and 7 post-day-60 IS (all P P P P Conclusion Early EPC administration provided better benefits on improving functional/image/molecular-cellular outcomes after acute IS in rat.

Published
2020

2. Combination of cilostazol and clopidogrel attenuates Rat critical limb ischemia

Author

Kun-Chen Lin, Cheuk-Kwan Sun, Ching-Yen Tsai, Steve Leu, Chia-Hung Yen, Yung-Lung Chen, Jiunn-Jye Sheu, Hsueh-Wen Chang, Hon-Kan Yip, Sarah Chua, Tzu-Hsien Tsai, and Jenq-Lin Yang

Subjects
Male, medicine.medical_specialty, Ticlopidine, Blotting, Western, Ischemia, Urology, lcsh:Medicine, Tetrazoles, Apoptosis, Inflammation, Real-Time Polymerase Chain Reaction, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Rats, Sprague-Dawley, Enos, medicine, Animals, Medicine(all), biology, Biochemistry, Genetics and Molecular Biology(all), business.industry, Research, lcsh:R, Critical limb ischemia, Extremities, General Medicine, Flow Cytometry, biology.organism_classification, medicine.disease, Immunohistochemistry, Cilostazol, Clopidogrel, Rats, body regions, Oxidative Stress, Anesthesia, Drug Therapy, Combination, Pharmacomodulation, Angiogenesis, medicine.symptom, business, Oxidative stress, medicine.drug
Abstract

Background and aim Procedural failure and untoward clinical outcomes after surgery remain problematic in critical limb ischemia (CLI) patients. This study tested a clopidogrel-cilostazol combination treatment compared with either treatment alone in attenuating CLI and improving CLI-region blood flow in rats. Methods Male Sprague–Dawley rats (n = 40) were equally divided into five groups: control, CLI induction only, CL I + cilostazol (12.0 mg/day/kg), CLI + clopidogrel (8.0 mg/kg/day) and CLI + combined cilostazol-clopidogrel. After treatment for 21 days, Laser Doppler imaging was performed. Results On day 21, the untreated CLI group had the lowest ratio of ischemic/normal blood flow (p Conclusion Combined cilostazol-clopidogrel therapy is superior to either agent alone in improving ischemia in rodent CLI.

Published
2012

3. Combination of cilostazol and clopidogrel attenuates Rat critical limb ischemia.

Author

Jiunn-Jye Sheu, Kun-Chen Lin, Ching-Yen Tsai, Tzu-Hsien Tsai, Leu, Steve, Chia-Hung Yen, Yung-Lung Chen, Hsueh-Wen Chang, Cheuk-Kwan Sun, Chua, Sarah, Jenq-Lin Yang, and Hon-Kan Yip

Subjects
*ISCHEMIA, *MESSENGER RNA, *IMMUNOCYTOCHEMISTRY, *NEOVASCULARIZATION, *BLOOD plasma
Abstract

Background and aim: Procedural failure and untoward clinical outcomes after surgery remain problematic in critical limb ischemia (CLI) patients. This study tested a clopidogrel-cilostazol combination treatment compared with either treatment alone in attenuating CLI and improving CLI-region blood flow in rats. Methods: Male Sprague-Dawley rats (n = 40) were equally divided into five groups: control, CLI induction only, CL I + cilostazol (12.0 mg/day/kg), CLI + clopidogrel (8.0 mg/kg/day) and CLI + combined cilostazol-clopidogrel. After treatment for 21 days, Laser Doppler imaging was performed. Results: On day 21, the untreated CLI group had the lowest ratio of ischemic/normal blood flow (p < 0.001). Inflammation measured by VCAM-1 protein expression; oxidative stress; PAI-1, MMP-9 and TNF-α mRNA expressions; and immunofluorescence staining (IF) of CD68+ cells was lower with combined treatment than with the other treatments, and lower in the two single-treatment groups than the untreated CLI group (all p < 0.01). Antiinflammatory mRNA expression of interleukin-10, and eNOS showed a reverse pattern among these groups. Apoptosis measured by Bax, caspase-3 and PARP; and muscle damage measured by cytosolic cytochrome-C, and serum and muscle micro-RNA-206 were all lowest with combination treatment, and the two single-treatment groups showed lower values than the untreated group (all p < 0.001). Angiogenesis measured by eNOS, IF staining of CD31+ and vWF + cells; and number of vessels in CLI region were highest with combination treatment and higher in the single-treatment groups than the untreated group (all p < 0.001). Conclusion: Combined cilostazol-clopidogrel therapy is superior to either agent alone in improving ischemia in rodent CLI. [ABSTRACT FROM AUTHOR]

Published
2012
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4. Combination of cilostazol and clopidogrel attenuates Rat critical limb ischemia

Author

Sheu Jiunn-Jye, Lin Kun-Chen, Tsai Ching-Yen, Tsai Tzu-Hsien, Leu Steve, Yen Chia-Hung, Chen Yung-Lung, Chang Hsueh-Wen, Sun Cheuk-Kwan, Chua Sarah, Yang Jenq-Lin, and Yip Hon-Kan

Subjects
Critical limb ischemia, Apoptosis, Inflammation, Pharmacomodulation, Angiogenesis, Medicine
Abstract

Abstract Background and aim Procedural failure and untoward clinical outcomes after surgery remain problematic in critical limb ischemia (CLI) patients. This study tested a clopidogrel-cilostazol combination treatment compared with either treatment alone in attenuating CLI and improving CLI-region blood flow in rats. Methods Male Sprague–Dawley rats (n = 40) were equally divided into five groups: control, CLI induction only, CL I + cilostazol (12.0 mg/day/kg), CLI + clopidogrel (8.0 mg/kg/day) and CLI + combined cilostazol-clopidogrel. After treatment for 21 days, Laser Doppler imaging was performed. Results On day 21, the untreated CLI group had the lowest ratio of ischemic/normal blood flow (p Conclusion Combined cilostazol-clopidogrel therapy is superior to either agent alone in improving ischemia in rodent CLI.

Published
2012
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