1. Antiangiogenic effects of phospholipase A 2 Lys49 BnSP-7 from Bothrops pauloensis snake venom on endothelial cells: An in vitro and ex vivo approach.
- Author
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Polloni L, Azevedo FVPV, Teixeira SC, Moura E, Costa TR, Gimenes SNC, Correia LIV, Freitas V, Yoneyama KAG, Rodrigues RS, Lopes DS, and Rodrigues VM
- Subjects
- Animals, Aorta drug effects, Bothrops, Cell Adhesion drug effects, Cell Line, Cell Movement drug effects, Cell Proliferation drug effects, Crotalid Venoms, Human Umbilical Vein Endothelial Cells metabolism, Human Umbilical Vein Endothelial Cells physiology, Humans, Mice, Vascular Endothelial Growth Factor A metabolism, Angiogenesis Inhibitors pharmacology, Group II Phospholipases A2 pharmacology, Human Umbilical Vein Endothelial Cells drug effects, Reptilian Proteins pharmacology
- Abstract
Antiangiogenic strategies are promising tools for cancer treatment and several other disorders. In this sense, phospholipases A
2 (PLA2 s) from snake venom have been described to possess antiangiogenic properties. In this study, we evaluated both in vitro and ex vivo antiangiogenic effects induced by BnSP-7, a Lys49 PLA2 isolated from Bothrops pauloensis snake venom. BnSP-7 was able to inhibit endothelial cell (HUVEC) proliferation, which was indeed confirmed by a modulation of cell cycle progression. Interestingly, BnSP-7 also inhibited the adhesion and migration of HUVECs and blocked in vitro angiogenesis in a VEGF-dependent manner, an important proangiogenic factor. Finally, BnSP-7 was capable of inhibiting sprouting angiogenic process through an ex vivo aortic ring assay. Taken together, these results indicate that BnSP-7 has potent in vitro and ex vivo antiangiogenic effect., (Copyright © 2021 Elsevier Ltd. All rights reserved.)- Published
- 2021
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