1. Central and peripheral slow-pressor mechanisms contributing to Angiotensin II-salt hypertension in rats.
- Author
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Lu J, Wang HW, Ahmad M, Keshtkar-Jahromi M, Blaustein MP, Hamlyn JM, and Leenen FHH
- Subjects
- Aldosterone blood, Animals, Brain metabolism, Cardenolides metabolism, Cytochrome P-450 CYP11B2 metabolism, Disease Models, Animal, Epithelial Sodium Channels metabolism, Hypertension chemically induced, Hypertension metabolism, Male, Rats, Wistar, Receptor, Angiotensin, Type 1 metabolism, Receptors, Mineralocorticoid metabolism, Saponins metabolism, Sodium-Calcium Exchanger metabolism, Sympathetic Nervous System metabolism, Time Factors, Angiotensin II, Arterial Pressure, Brain physiopathology, Cardiovascular System innervation, Hypertension physiopathology, Renin-Angiotensin System, Sodium Chloride, Dietary, Sympathetic Nervous System physiopathology
- Abstract
Aims: High salt intake markedly enhances hypertension induced by angiotensin II (Ang II). We explored central and peripheral slow-pressor mechanisms which may be activated by Ang II and salt., Methods and Results: In protocol I, Wistar rats were infused subcutaneously with low-dose Ang II (150 ng/kg/min) and fed regular (0.4%) or high salt (2%) diet for 14 days. In protocol II, Ang II-high salt was combined with intracerebroventricular infusion of mineralocorticoid receptor (MR) blockers (eplerenone, spironolactone), epithelial sodium channel (ENaC) blocker (benzamil), angiotensin II type 1 receptor (AT1R) blocker (losartan) or vehicles. Ang II alone raised mean arterial pressure (MAP) ∼10 mmHg, but Ang II-high salt increased MAP ∼50 mmHg. Ang II-high salt elevated plasma corticosterone, aldosterone and endogenous ouabain but not Ang II alone. Both Ang II alone and Ang II-high salt increased mRNA and protein expression of CYP11B2 (aldosterone synthase gene) in the adrenal cortex but not of CYP11B1 (11-β-hydroxylase gene). In the aorta, Ang II-high salt increased sodium-calcium exchanger-1 (NCX1) protein. The Ang II-high salt induced increase in MAP was largely prevented by central infusion of MR blockers, benzamil or losartan. Central blockades significantly lowered plasma aldosterone and endogenous ouabain and markedly decreased Ang II-high salt induced CYP11B2 mRNA expression in the adrenal cortex and NCX1 protein in the aorta., Conclusion: These results suggest that in Ang II-high salt hypertension, MR-ENaC-AT1R signalling in the brain increases circulating aldosterone and endogenous ouabain, and arterial NCX1. These factors can amplify blood pressure responses to centrally-induced sympatho-excitation and thereby contribute to severe hypertension., (Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2017. For permissions please email: journals.permissions@oup.com.)
- Published
- 2018
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