1. Alpha1beta1 and integrin-linked kinase interact and modulate angiotensin II effects in vascular smooth muscle cells.
- Author
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Moraes JA, Frony AC, Dias AM, Renovato-Martins M, Rodrigues G, Marcinkiewicz C, Assreuy J, and Barja-Fidalgo C
- Subjects
- Acetophenones pharmacology, Animals, Aorta, Thoracic drug effects, Aorta, Thoracic enzymology, Cells, Cultured, Cyclin-Dependent Kinase Inhibitor p21 metabolism, Enzyme Inhibitors pharmacology, Focal Adhesion Kinase 1 metabolism, Integrin alpha1beta1 antagonists & inhibitors, Male, Membrane Glycoproteins antagonists & inhibitors, Membrane Glycoproteins metabolism, Muscle, Smooth, Vascular enzymology, Myocytes, Smooth Muscle enzymology, NADH, NADPH Oxidoreductases genetics, NADH, NADPH Oxidoreductases metabolism, NADPH Oxidase 1, NADPH Oxidase 2, NADPH Oxidases antagonists & inhibitors, NADPH Oxidases metabolism, Phosphorylation, Protein Serine-Threonine Kinases genetics, Proteolysis, Proto-Oncogene Proteins c-akt metabolism, RNA Interference, Rats, Wistar, Reactive Oxygen Species metabolism, Signal Transduction drug effects, Time Factors, Transfection, Viper Venoms pharmacology, Angiotensin II pharmacology, Cell Movement drug effects, Cell Proliferation drug effects, Integrin alpha1beta1 metabolism, Muscle, Smooth, Vascular drug effects, Myocytes, Smooth Muscle drug effects, Protein Serine-Threonine Kinases metabolism
- Abstract
The effects of angiotensin II (Ang II) on vascular smooth muscle cells (VSMC) are modulated by reactive oxygen species (ROS) and also involve integrin engagement. However, the potential link between alpha1beta1 integrin signaling with NOX system and their combined contribution to Ang II effects on VSMC have not been investigated. We aimed to elucidate the moslecular mechanisms underlying the activation of these two pathways in Ang II effects on VSMC. Ang II-induced VSMC migration (2-fold increase) and proliferation (2.5-fold increase) is modulated by alpha1beta1 integrin, being inhibited by obtustatin, a specific alpha1beta1 integrin blocker. Ang II also stimulates ROS production in VSMC (140%) that is NOX1 dependent, being completely inhibited in NOX1 silenced cells. The ROS production develops in two peaks, and the second peak is maintained by NOX2 activation. Apocynin and obtustatin inhibit the NOX2-associated second peak, but not the first peak of ROS production, which is related to NOX1 activation. Corroborating the involvement of alpha1beta1 integrin, the pretreatment of VSMC with obtustatin impaired Ang II-induced FAK phosphorylation, AKT activation, p21 degradation and the increase of ILK expression. Silencing of ILK blocked cell migration, AKT phosphorylation and the second peak of ROS, but partially inhibits (70%) VSMC proliferation induced by Ang II. The data demonstrate a novel role for NOX2 in Ang II effects on VSMC, and suggest alpha1beta1 integrin and ILK as target molecules to the development of more effective therapeutic interventions in cardiovascular diseases., (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2015
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