1. Immunogenicity of Intensively Decellularized Equine Carotid Arteries Is Conferred by the Extracellular Matrix Protein Collagen Type VI
- Author
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Heiko Meyer, Falk F. R. Buettner, Ulrike Boeer, Mathias Wilhelmi, Georgios C. Antonopoulos, Axel Haverich, and Melanie Klingenberg
- Subjects
Proteomics ,Pathology ,Cardiovascular Procedures ,immunogenicity ,Biochemistry ,Mice ,Tissue engineering ,Animal Cells ,Collagen VI ,lcsh:Science ,chemistry.chemical_classification ,epitope ,Tissue Scaffolds ,Stem Cells ,Immunogenicity ,horse ,tissue engineering ,Cytochemistry ,ddc:500 ,Cellular Structures and Organelles ,Cellular Types ,Collagen Type IV ,medicine.medical_specialty ,Immune Cells ,two dimensional gel electrophoresis ,Biomedical Engineering ,Bioengineering ,Surgical and Invasive Medical Procedures ,animal tissue ,Biomaterials ,biocompatibility ,liquid chromatography ,Horses ,cell protein ,xenograft ,mouse ,volumetry ,Blood Cells ,carotid artery ,lcsh:R ,Biology and Life Sciences ,Proteins ,Epithelial Cells ,DNA ,Equidae ,Biological Tissue ,chemistry ,smooth muscle actin ,Medical Devices and Equipment ,lcsh:Q ,Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit ,transplantation ,lcsh:Medicine ,major histocompatibility antigen class 1 ,Matrix (biology) ,immunoprecipitation ,Epithelium ,immunology ,Extracellular matrix ,Medicine and Health Sciences ,deoxycholate sodium ,animal ,histocompatibility ,Dewey Decimal Classification::500 | Naturwissenschaften ,mass spectrometry ,antibody production ,Prosthetics ,Immune System Proteins ,Multidisciplinary ,Decellularization ,tissue scaffold ,scleroprotein ,article ,female ,Carotid Arteries ,Heterografts ,Anatomy ,Research Article ,Biotechnology ,extracellular matrix ,immunization ,Antibodies ,collagen type 4 ,Transplantation Immunology ,collagen type 6 ,parasitic diseases ,medicine ,Animals ,controlled study ,ddc:610 ,artery wall ,nonhuman ,Scleroprotein ,Cell Biology ,Molecular biology ,Transplantation ,DNA content ,protein analysis ,Clinical Immunology ,Pericytes ,clinical protocol - Abstract
The limited biocompatibility of decellularized scaffolds is an ongoing challenge in tissue engineering. Here, we demonstrate the residual immunogenicity of an extensively decellularized equine carotid artery (dEAC intens) and identify the involved immunogenic components. EAC were submitted to an elaborated intensified decellularization protocol with SDS/sodium desoxycholate for 72 h using increased processing volumes (dEAC intens), and compared to dEACord prepared by an ordinary protocol (40 h, normal volumes). Matrix integrity was checked via correlative volumetric visualization which revealed only minor structural changes in the arterial wall. In dEACintens, a substantial depletion of cellular components was obvious for smooth muscle actin (100%), MHC I complexes (97.8%), alphaGal epitops (98.4% and 91.3%) and for DNA (final concentration of 0.34±0.16 ng/mg tissue). However, dEACintens still evoked antibody formation in mice after immunization with dEACintens extracts, although to a lower extent than dEACord. Mouse plasma antibodies recognized a 140 kDa band which was revealed to contain collagen VI alpha1 and alpha2 chains via mass spectrometry of both 2D electrophoretically separated and immunoprecipitated proteins. Thus, even the complete removal of cellular proteins did not yield non-immunogenic dEAC as the extracellular matrix still conferred immunogenicity by collagen VI. However, as lower antibody levels were achieved by the intensified decellularization protocol, this seems to be a promising basis for further development.
- Published
- 2014
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