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The objective of this study is to investigate the effects of increasing levels of soybean hulls in broiler chicken diets on growth performance, carcase composition, organ weights, nutrient digestibility, amino acid digestibility, and intestinal histomorphology to determine the optimal inclusion level for maximising poultry health and performance. A total of 680, 1-day-old male broiler chicks (Hubbard) were randomly assigned to five treatments with eight replicates, each consisting of seventeen birds, and housed in an environmentally controlled room with nipple drinkers and trough feeders. The experimental diets, containing 0%, 2%, 4%, 6%, and 8% dietary soybean hulls (SH), were fed to the broilers for 35 days. During the starter phase, the growth performance of chickens fed 2% SH was not significantly different from the control group, but differences emerged during the later stages. Chickens fed 2% SH showed significantly higher body weight and weight gain compared to higher SH levels. Carcase yield decreased with increasing SH content in the diet, while wing meat yield was highest in birds fed 0% and 2% SH. The relative weights of gizzard, jejunum, and ileum varied significantly among groups. Higher SH levels led to decreased nutrient digestibility but increased excreta nitrogen and ether extract content. Ileal amino acid digestibility varied among SH levels. Intestinal histomorphology revealed significant differences in villi height and crypt depth among groups. Overall, diets with 2% and 4% SH demonstrated superior nutrient utilisation and intestinal health compared to higher SH levels. These findings suggest an optimal inclusion level of SH in broiler diets to optimise performance and intestinal morphology.

A total of 576 rabbits were housed in 18 open-top elevated pens (32 rabbits per pen) during the fattening period (54–71 d of age) and fed a standard diet or a diet enriched in purified wood lignocellulose and fat formulated to guarantee rabbit optimal digestive health and convenient feed conversion ratio. The impacts on gut microbiota (both hard faeces and caecal content) and caecal fermentation, diet digestibility and rabbit performance were examined. The microbial diversity and the bacterial community structure of hard faeces barely differed from that of caecal content (p > .05). No differences in alpha and beta diversity of microbiota were detected between rabbits fed the different diets. Twelve genera, mostly belonging to the family of Lachnospiraceae, increased (p

Abstract Background Feed efficiency is a crucial economic trait in poultry industry. Both host genetics and gut microbiota influence feed efficiency. However, the associations between gut microbiota and host genetics, as well as their combined contributions to feed efficiency in laying hens during the late laying period, remain largely unclear. Methods In total, 686 laying hens were used for whole-genome resequencing and liver transcriptome sequencing. 16S rRNA gene sequencing was conducted on gut chyme (duodenum, jejunum, ileum, and cecum) and fecal samples from 705 individuals. Bioinformatic analysis was performed by integrating the genome, transcriptome, and microbiome to screen for key genetic variations, genes, and gut microbiota associated with feed efficiency. Results The heritability of feed conversion ratio (FCR) and residual feed intake (RFI) was determined to be 0.28 and 0.48, respectively. The ileal and fecal microbiota accounted for 15% and 10% of the FCR variance, while the jejunal, cecal, and fecal microbiota accounted for 20%, 11%, and 10% of the RFI variance. Through SMR analysis based on summary data from liver eQTL mapping and GWAS, we further identified four protein-coding genes, SUCLA2, TNFSF13B, SERTM1, and MARVELD3, that influence feed efficiency in laying hens. The SUCLA2 and TNFSF13B genes were significantly associated with SNP 1:25664581 and SNP rs312433097, respectively. SERTM1 showed significant associations with rs730958360 and 1:33542680 and is a potential causal gene associated with the abundance of Corynebacteriaceae in feces. MARVELD3 was significantly associated with the 1:135348198 and was significantly correlated with the abundance of Enterococcus in ileum. Specifically, a lower abundance of Enterococcus in ileum and a higher abundance of Corynebacteriaceae in feces were associated with better feed efficiency. Conclusions This study confirms that both host genetics and gut microbiota can drive variations in feed efficiency. A small portion of the gut microbiota often interacts with host genes, collectively enhancing feed efficiency. Therefore, targeting both the gut microbiota and host genetic variation by supporting more efficient taxa and selective breeding could improve feed efficiency in laying hens during the late laying period.

Residual feed intake (RFI) is a metric that provides a more accurate measure of feed efficiency. The lower the RFI, the higher the feed efficiency. The changes in the host microbiome and metabolome contribute to the greater feed efficiency of low RFI (LRFI) animals. The aim of this study was to explore the differences in rumen microorganisms, rumen metabolites and plasma metabolites of Hu sheep with differing RFI through the microbiome and metabolome. A total of 80 Hu sheep were used. The experiment consisted of a 15-d pretrial period and a 128-d experimental period. The RFI in the experimental period was calculated for all sheep, and the sheep were screened into high RFI (HRFI, n = 8) and LRFI (n = 8) groups. The HRFI and LRFI sheep did not differ in their initial and final body weights, average daily gain and body measurements, but the dry matter intake of LRFI sheep was significantly decreased (28.4%, P

A key aspect to optimise the Tenebrio molitor (TM) farm productivity is to find an optimal breeding density for adults. To this purpose, this study investigated, from an industrial perspective, the impact of four breeding densities (D1, D2, D3 and D4, equal to 0.8, 1.1, 1.3 and 1.6 adults/cm2, respectively) on mortality and reproductive performances of TM adults and larvae. Two weeks after pupae emergence, TM adults were randomly assigned to the four groups and housed in 48 breeding crates (60 × 40 × 14.5 cm; 12 crates/group). The trial consisted of 4 consecutive weeks of adult breeding (each week represented one oviposition), and 8 weeks of larvae growth. From each week of oviposition, a batch of larvae was obtained for a total of four batches of larvae (48 crates/batch). Larvae of each batch were grown until 8 weeks of age, corresponding to the period required to reach the selling size. Larvae were kept in the original crates until the 5th weeks of age, after which they were divided into additional crates to ensure a density of 4.2 larvae/cm2 and a final weight of 1 500 g of larvae/crate (0.6 g of larvae/cm2). For each oviposition week, TM adult’s mortality, egg hatchability, BW, feed intake, chemical composition and fatty acid profile were evaluated, while ovary weight was measured at weeks 1 and 4. The number of larvae was monitored at week 5 of age while their chemical composition was determined at week 5 and 8. Larvae feed intake and BW were monitored over the 8-weeks growth period. Individual feed intake and BW of adults increased with increasing breeding density (P

The feed consumption and feeding patterns of group-housed livestock and their productive outputs can be affected by interactions among group members, such as competition for feeder access and aggression. The impact of competition is especially significant when feed restriction is implemented during the fattening stage, a common practice in rabbit farms to reduce mortality from digestive disorders during this period. The overall aim was to quantify the importance of social interaction effects at the genetic and environmental level on feed efficiency, feeding behaviour and social ranking traits in rabbit populations having access to electronic feeders whilst they were housed in groups during fattening. This was done by quantifying novel feeding behaviour (FB) and social ranking (SR) traits and by estimating the genetic parameters and genetic correlations between all combinations of direct and social effects of these traits and residual feed intake (RFI), an indicator of feed efficiency. These effects were estimated in animals fed either ad libitum (AL) or on a time-based feed restriction (R) and were selected for feed efficiency on these feeding regimes for six generations. We found that there were substantial social genetic and social litter effects on RFI and FB traits, manifesting as antagonistic relationships between direct and social genetic effects. These effects were stronger amongst the R, as opposed to the AL animals. Despite these antagonistic relationships, the large social genetic effects resulted in substantial heritable variation available for selection of these traits, and therefore feeding behaviour could be changed by genetic selection, especially for R animals. The indicators of an animal’s SR were also heritable and subject to social genetic effects and therefore may respond to selection. However, the phenotypic population mean would not change, because such traits are context−specific. The animals that took most of the feeding resources and had priority access to the feeder had a higher RFI, that is, they were less efficient, but they made their cage mates more efficient. This is the first time that the genetic correlations between direct effects of RFI and direct effects of FB and SR traits, as well as between social effects of RFI and direct effects of FB traits and SR traits, have been quantified. Results could help to understand the mechanisms by which an animal exerts its influence on the feed efficiency of its cage mates under different feeding regimes and assist in the development of breeding strategies.

Identification of plasma biomarkers for feed efficiency in growing beef cattle offers a promising opportunity for developing prediction models to improve precision feeding strategies. However, these models must accurately predict feed efficiency at early stages of fattening. Our study aimed to evaluate the reliability of candidate biomarkers previously identified in late-fattening cattle when analysed during early fattening stages and to develop diet-specific prediction equations for residual feed intake (RFI). From a total of 364 Charolais bulls across seven cohorts, we selected 64 animals with extreme RFI values. The animals were fed either a corn‑ or grass-silage diets. These animals were chosen from four out of the available seven cohorts. Animals from three cohorts (24 high-RFI and 24 low-RFI, having a mean RFI difference of 1.48 kg/d) were used for biomarker confirmation and prediction model training. Animals from a fourth cohort (8 high-RFI and 8 low-RFI, having a mean RFI difference of 0.98 kg/d) were used for model external validation. Blood samples were collected at the beginning of the feed efficiency test (333 ± 20 days), and plasma underwent targeted metabolomic for 630 metabolites, natural abundance of 15N (δ15N), insulin, and IGF-1 analysis. Seven previously identified plasma biomarkers for RFI in late-fattening beef cattle still kept their capability for discriminating low and high RFI animals when analysed during early fattening stages (P 0.05). Prediction models were developed based on seven confirmed RFI biomarkers analysed during early-fattening. Two logistic regression models incorporating creatinine and either IGF-1 (for grass silage-based diet) or PC aa C30:2 (for corn silage-based diet) effectively distinguished between high− and low-RFI animals with high sensitivity and specificity (area under the curve > 0.80). The biomarkers used in the models showed moderate to high repeatability between early and late fattening stages (0.45

ABSTRACT: Variation in body weight (BW) within broiler flocks is a significant challenge in poultry production. Investigating differences in gut-related parameters between low (LBW) and high BW (HBW) chicks may provide insights into the underlying causes of BW heterogeneity. 908 day-old male broiler chicks were reared until d 7 and then ranked into LBW and HBW groups. Thereafter, performance parameters were compared between BW groups periodically. On d 7, 14, and 38, visceral organ characteristics, intestinal permeability, and duodenal and ileal histomorphology were examined. Expression profiles were analyzed for 79 ileal genes related to gut barrier function, immune function, nutrient transport, gut hormones, nutrient receptors, metabolism, and oxidation using high-throughput qPCR. Student's t-tests were performed to compare measurements. Multivariate statistics, including partial least square regression (PLSR) analysis, were applied to identify combinations of key genes discriminating BW groups, offering predictive capability for phenotypic variations. The HBW group remained heavier at each timepoint, which could be explained by higher feed intake. The HBW group had shorter relative small intestine length but higher villus height and villi height/crypt depth ratios. The LBW group demonstrated increased intestinal permeability on d 38. The LBW group showed upregulation of immune response genes including TNF-α on d 7 and CYP450 on d 38, while the HBW group showed higher AHSA1 and HSPA4 expressions on d 7. The LBW group had upregulation of the metabolism genes mTOR and EIF4EBP1 on d 7 and the satiety-induced hormone cholecystokinin on d 14, while the HBW group tended to increase expression of the hunger hormone ghrelin on d 38. Genes related to gut barrier function, nutrient transport, and oxidation categories were consistently upregulated in the HBW group. PLSR models revealed 4, 12, and 11 sets of key genes highly predictive of BW phenotypes on d 7, 14, and 38, respectively. These findings suggest that growth rates are linked to the intestinal size, structure, and function of broiler chickens, offering insights into the underlying mechanisms regulating BW.

In markets for beef and sheep meat, an appropriate level of intramuscular fat (IMF) is highly desirable for meat-eating quality, but strategies to improve it usually lead to an undesirable excess in carcase fat, presenting a major challenge to livestock producers. To solve this problem, we need to understand the partitioning of fat among the major fat depots: IMF, subcutaneous fat (SCF) and visceral fat (VF). In most genotypes of cattle and sheep, the rate of accretion is lower for IMF than for SCF and VF, so genetic selection for a high level of IMF, or the use of an increased dietary energy supply to promote IMF deposition, will increase overall fatness and feed costs. On the other hand, feeding postnatal calves with excessive concentrates promotes IMF deposition, so a nutritional strategy is feasible. With genetic strategies, several problems arise: 1) positive genetic correlations between IMF, SCF and VF differ among genotypes in both cattle and sheep; 2) genotypes appear to have specific, characteristic rates of accretion of IMF during periods of growth and fattening; 3) most breeds of cattle and sheep naturally produce meat with relatively low levels of IMF, but IMF does vary substantially among individuals and breeds so progress is possible through accurate measurement of IMF. Therefore, an essential prerequisite for selection will be knowledge of the genetic correlations and fat accretion rates for each genotype. Currently, selection for IMF is based on existing technology that directly measures IMF in the progeny or siblings, or estimates IMF in live animals. New technology is needed to permit the simultaneous measurement of SCF and IMF in the field, thus opening up the possibility of accurate selection, particularly for fat partitioning in live animals. Specifically, there would be great value in detecting individuals with an IMF advantage at an early age so the generation interval could be shortened and genetic gain accelerated. Genetic gain would also be greatly aided if we could select for genes that control adipogenesis and lipogenesis and are also differentially expressed in the various depots.

"Nano", which derives from the Latin word nanus and means dwarf, refers to a very small unit of measurement equal to one billionth of a meter. Nanotechnology, which deals with the manipulation of matter at the atomic and molecular level, has an application area in animal husbandry as well as in many fields. Nano-sized feed additives, which have come to the forefront in the livestock sector in recent years, have become an innovative application used to increase the nutritional value of feeds and optimize animal health and performance. Since these additives are nano-sized particles with increased specific surface area, they can have a positive effect on a number of factors such as digestibility, nutrient absorption, immune system, growth and development. Minerals in the form of nanoparticles used as feed additives can increase bioavailability by passing through the intestinal wall to body cells faster compared to larger particles. The nano level of the substance not only increases the productivity of animals, but also brings the potential to improve the functionality of feed molecules. Nano feed additives increase the digestion and absorption of feed, allowing animals to benefit from feed more effectively. However, there are several challenges associated with this approach. These include the potential for endotoxin production, reduced nutrient absorption due to interaction with natural nutrients, the possibility of nanoparticle accumulation in the animal body, health risks, ethical considerations, environmental concerns and some negative effects such as interference with natural nutrients that can be avoided by encapsulation. This article discusses recent studies on nano-sized feed additives that offer potential benefits in animal nutrition. [ABSTRACT FROM AUTHOR]

The residual feed intake (RFI) model has recently gained popularity for ranking dairy cows for feed efficiency. The RFI model ranks the cows based on their expected feed intake compared to the observed feed intake, where a negative phenotype (eating less than expected) is favourable. Yet interpreting the biological implications of the regression coefficients derived from RFI models has proven challenging. In addition, multitrait modelling of RFI has been proposed as an alternative to the least square RFI in nutrition and genetic studies. To solve the challenge with the biological interpretation of RFI regression coefficients and suggest ways to improve the modelling of RFI, an interdisciplinary effort was required between nutritionists and geneticists. Therefore, this paper aimed to explore the challenges with the traditional least square RFI model and propose solutions to improve the modelling of RFI. In the traditional least square RFI model, one set of fixed effects is used to solve systematic effects (e.g., seasonal effects and age at calving) for traits with different means and variances. Thereby, measurement and model fitting errors can accumulate in the phenotype, resulting in undesirable effects. A multivariate RFI model will likely reduce this problem, as trait-specific fixed effects are used. In addition, regression coefficients for DM intake on milk energy tend to have more biologically meaningful estimates in multitrait RFI models, which indicates a confounding effect between the fixed effects and regression coefficients in the least square RFI model. However, defining precise expectations for regression coefficients from RFI models or sourcing for accurate feed norm coefficients seems difficult, especially if the coefficients are applied to a wide cattle population with varying diets or management systems, for example. To improve multitrait modelling of RFI, we suggest improving the modelling of changes in energy status. Furthermore, a novel method to derive the energy density of the diet and individual digestive efficiency is proposed. Digestive efficiency is defined as the part of the efficiency associated with digestive processes, which primarily reflects the conversion from gross energy to metabolisable energy. We show the model was insensitive to prior values of energy density in feed and that there was individual variation in digestive efficiency. The proposed method needs further development and validation. In summary, using multitrait RFI can improve the accuracy of the ranking of dairy cows’ feed efficiency, consequently improving economic and environmental sustainability on dairy farms.

The mule duck accounts for over 90% of French foie gras production, a sector where feed represents two-thirds of production costs. This study focuses on analysing the feeding behaviours of the mule duck and its parental populations (Pekin and Muscovy) using automated feeders. To assess feed efficiency, feed conversion ratio and residual feed intake were analysed, along with six traits derived at the daily and meal levels. Genetic parameters were estimated separately in purebred populations, as well as with a joint crossbred model that estimated the parental contributions to the hybrid crossbred performances. In relation to higher feed intakes and much−reduced feeding times (P 0.41 ± 0.20) for fatty liver weight. Altogether, improving liver traits and feed efficiency in the hybrid through selection in the parental populations could be enhanced by considering feeding traits recorded with electronic feeders, provided that adverse correlations are properly accounted for in a multitrait index.

In recent years, rising prices for high-quality protein-based feeds have significantly increased nutrition costs. Consequently, investigating strategies to reduce these expenses and improve feed efficiency (FE) have become increasingly important for the dairy sheep industry. This research investigates the impact of nutritional protein restriction (NPR) during prepuberty and FE on the milk transcriptome of dairy Assaf ewes (sampled during the first lactation). To this end, we first compared transcriptomic differences between NPR and control ewes. Subsequently, we evaluated gene expression differences between ewes with divergent FE, using feed conversion ratio (FCR), residual feed intake (RFI), and consensus classifications of high− and low-FE animals for both indices. Lastly, we assess milk gene expression as a predictor of FE phenotype using random forest. No effect was found for the prepubertal NPR on milk performance or FE. Moreover, at the milk transcriptome level, only one gene, HBB, was differentially expressed between the NPR (n = 14) and the control group (n = 14). Further, the transcriptomic analysis between divergent FE sheep revealed 114 differentially expressed genes (DEGs) for RFI index (high-FERFI = 10 vs low-FERFI = 10), 244 for FCR (high-FEFCR = 10 vs low-FEFCR = 10), and 1 016 DEGs between divergent consensus ewes for both indices (high-FEconsensus = 8 vs low-FEconsensus = 8). These results underscore the critical role of selected FE indices for RNA-Seq analyses, revealing that consensus divergent animals for both indices maximise differences in transcriptomic responses. Genes overexpressed in high-FEconsensus ewes were associated with milk production and mammary gland development, while low-FEconsensus genes were linked to higher metabolic expenditure for tissue organisation and repair. The best prediction accuracy for FE phenotype using random forest was obtained for a set of 44 genes consistently differentially expressed across lactations, with Spearman correlations of 0.37 and 0.22 for FCR and RFI, respectively. These findings provide insights into potential sustainability strategies for dairy sheep, highlighting the utility of transcriptomic markers as FE proxies.

ABSTRACT: Feed efficiency (FE) is a crucial economic indicator of meat duck production. The objective of this study was to assess the impact of residual feed intake (RFI), defined as the difference between the actual and expected feed intake based on animal's production and maintenance requirements, on the growth performance (GP), slaughter and internal organ characteristics of fast-growing meat ducks. In total, 1,300 healthy 14-day-old male fast-growing meat ducks were housed in individual cages until slaughter at the age of 35 d. The characteristics of the carcass and internal organs of 30 ducks with the highest RFI (HRFI) and the lowest RFI (LRFI) were respectively determined. RFI, the feed conversion ratio (FCR), and average day feed intake (ADFI) were significantly lower in the LRFI group than the HRFI group (P < 0.001), while there were no significant differences in marketing BW or BW gain (BWG) (P > 0.05). The thigh muscle and lean meat yields were higher, and the abdominal fat content was lower (P < 0.001) in the LRFI group, while there were no significant differences in other carcass traits between the groups (P > 0.05). The liver and gizzard yields were significantly higher (P < 0.001) in the LRFI group, while there were no significant differences (P > 0.05) in intestinal length between the groups. RFI was highly positively correlate with FCR and ADFI (P < 0.01), but negatively correlated the yields of thigh muscle, lean meat, liver, and gizzard, and positively correlated with abdominal fat content. These results indicate that selection for low RFI could improve the FE of fast-growing meat ducks without affecting the marketing BW and BWG, while increasing yields of thigh muscle and lean meat and reducing abdominal fat content. These findings offer useful insights into the biological processes that influence FE of fast-growing meat ducks.

ABSTRACT: Heterosis has been widely utilized in chickens. The nonadditive inheritance of genes contributes to this biological phenomenon. However, the role of circRNAs played in the heterosis is poorly determined. In this study, we observed divergent heterosis for residual feed intake (RFI) between 2 crossbreds derived from a reciprocal cross between White Leghorns and Beijing You chickens. Then, circRNA landscape for 120 samples covering the hypothalamus, liver, duodenum mucosa and ovary were profiled to elucidate the regulatory mechanisms of heterosis. We detected that a small proportion of circRNAs (7.83–20.35%) were additively and non-additively expressed, in which non-additivity was a major inheritance of circRNAs in the crossbreds. Tissue-specific expression of circRNAs was prevalent across 4 tissues. Weighted gene co-expression network analysis revealed circRNA-mRNA co-expression modules associated with feed intake and RFI in the hypothalamus and liver, and the co-expressed genes were enriched in oxidative phosphorylation pathway. We further identified 8 nonadditive circRNAs highly correlated with 16 nonadditive genes regulating negative heterosis for RFI in the 2 tissues. Circ-ITSN2 was validated in the liver tissue for its significantly positive correlation with PGPEP1L. Moreover, the bioinformatic analysis indicated that candidate circRNAs might be functioned by binding the microRNAs and interacting with the RNA binding proteins. The integration of multi-tissue transcriptome firstly linked the association between tissue-specific circRNAs and the heterosis for feed intake and efficiency in chicken, which provide novel insights into the molecular mechanism underlying heterosis for feed efficiency. The validated circRNAs can act as potential biomarkers for predicting RFI and its heterosis.

ABSTRACT: This study aimed to estimate genetic parameters for feeding behavior (FB) traits and to assess their genetic relationship with performance traits in group-housed broilers. In total, 99,472,151 visits were recorded for 95,711 birds between 2017 and 2022 using electronic feeders. The visits were first clustered into 2,667,617 daily observations for ten FB traits: daily feed intake (DFI), daily number of visits (NVIS), time spent at the feeders (TSF), number of visited feeders (NVF), visiting activity interval (VAI), feeding rate (FR), daily number of meals (NMEAL), average intake per meal (INTMEAL), number of visits per meal (VISMEAL) and interval between meals (MEALIVL). All FB traits were then considered as the average per bird across the feeding test period. Three growth traits (body weight at the start – SBW and at the end of the feeding test – FBW, and weight gain over the test period – BWG), and 2 feed efficiency (FE) traits (Feed Conversion Rate - FCR and Residual Feed Intake - RFI) were also recorded. The (co)variance components were estimated using multitrait animal mixed models. For growth and FE, the heritability (h2) estimates were moderate, ranging from 0.20 ± 0.01 (BWG) to 0.32 ± 0.02 (RFI). Overall, the h2 estimates for FB traits were higher than for productive traits, ranging from 0.31 ± 0.01 (DFI) to 0.56 ± 0.02 (TSF). DFI presented high genetic correlations (0.53–0.86) with all performance traits. Conversely, the remaining FB traits presented null to moderate genetic correlations with these traits, ranging from –0.38 to 0.42 for growth traits and between –0.14 and 0.25 for FE traits. Genetic selection for favorable feeding behavior is expected to exhibit a fast genetic response. The results suggest that it is possible to consider different feeding strategies without compromising the genetic progress of FE. Conversely, breeding strategies prioritizing a higher bird activity might result in lighter broiler lines in the long term, given the negative genetic correlations between visit-related traits (NV, NVF, and NMEAL) and growth traits (SBW and FBW).

In this research, the growth efficiency, nutritional utilization, fecal microbial levels, and fecal score of weaned pigs were evaluated using therapeutic zinc oxide (ZnO) and zinc aspartic acid chelate (Zn-Asp). In a 42-day feeding trial, 60 weaned pigs ([Yorkshire × Landrace] × Duroc) were arbitrarily allotted (age: 21 days; 7.01 ± 0.65 kg preliminary body weight) to 3 different treatment groups with 5 repetitions (2 male and 2 female piglets) in each pen. The trial had 2 different phases, including 1–21 days as phase 1, and 22–42 days as phase 2. The nutritional treatments were: basal diet as control (CON), basal diet incorporated with 3,000 ppm ZnO as TRT1, and basal diet incorporated with 750 ppm Zn-Asp as TRT2. In comparison to the CON group, the pigs in the TRT1 and TRT2 groups had greater (p < 0.05) body weight on day 42; an average daily gain, and an average daily feed intake on days 22–42. Furthermore, during days 1-42, the average daily gain in the treatment groups trended higher (p < 0.05) than in the CON group. Additionally, the fecal score decreased (p < 0.05) at week 6, the lactic acid bacteria count tended to increase (p < 0.05), and coliform bacteria presented a trend in reduction (p < 0.05) in the TRT1 and TRT2 groups compared to the CON group. However, there was no difference in nutrient utilization (p > 0.05) among the dietary treatments. Briefly, the therapeutic ZnO and Zn-Asp nutritional approaches could decrease fecal score and coliform bacteria, increase lactic acid bacteria, and improve growth efficiency; moreover, Zn-Asp (750 ppm) can perform a comparable role to therapeutic ZnO (3,000 ppm). So we can use Zn-Asp (750 ppm) instead of therapeutic ZnO (3,000 ppm) for the better performance of weaning pigs and the reduction of environmental pollution, as therapeutic ZnO is responsible for environmental pollution.

Measuring feed intake accurately is crucial to determine feed efficiency and for genetic selection. A system using three-dimensional (3D) cameras and deep learning algorithms can measure the volume of feed intake in dairy cows, but for now, the system has not been validated for feed intake expressed as weight of feed. The aim of this study was to validate the weight of feed intake predicted from the 3D cameras with the actual measured weight. It was hypothesised that diet−specific coefficients are necessary for predicting changes in weight, that the relationship between weight and volume is curvilinear throughout the day, and that manually pushing the feed affects this relationship. Twenty-four lactating Danish Holstein cows were used in a cross-over design with four dietary treatments, 2 × 2 factorial arranged with either grass-clover silage or maize silage as silage factor, and barley or dried beet pulp as concentrate factor. Cows were adapted to the diets for 11 d, and for 3 d to tie-stall housing before camera measurements. Six cameras were used for recording, each mounted over an individual feeding platform equipped with a weight scale. When building the predictive models, four cameras were used for training, and the remaining two for testing the prediction of the models. The most accurate predictions were found for the average feed intake over a period when using the starting density of the feed pile, which resulted in the lowest errors, 6% when expressed as RMSE and 5% expressed as mean absolute error. A model including curvilinear effects of feed volume and the impact of manual feed pushing was used on a dataset including daily time points. When cross-validating, the inclusion of a curvilinear effect and a feed push effect did not improve the accuracy of the model for neither the feed pile nor the feed removed by the cow between consecutive time points. In conclusion, measuring daily feed intake from this 3D camera system in the present experimental setup could be accomplished with an acceptable error (below 8%), but the system should be improved for individual meal intake measurements if these measures were to be implemented.

ABSTRACT: Light flicker is a commonly overlooked factor of artificial light sources. This study aimed to determine the impacts of light-flicker frequency on performance, general health, and mortality of 11-wk Nicholas Select turkey hens. The experiment consisted of 2 trials (block) in a randomized complete block design, with 3 light-flicker frequency treatments (30, 90, or 195 Hz). Turkeys (n = 364 per replicate) were randomly placed into environmentally controlled rooms (3 room replicates per treatment per trial). Group body weight (BW) and feed consumption were measured at 0, 4, 8, and 11 wk, and feed efficiency (mortality corrected feed-to-gain; F:Gm) was calculated for each period. Mortality and culls were collected twice daily. Flock uniformity, feather condition and cleanliness, footpad score, and mobility were evaluated at 10 wk (30 birds per room). Litter quality and ocular weight and dimensions were evaluated (11 wk; 4 birds per room). Data were analyzed using Proc Mixed (SAS 9.4) and significance was declared when P < 0.05. At 8 wk, BW was lower under 30 Hz compared to 195 Hz (P = 0.03). Feed consumption was lowest under 30 Hz (0–4 wk and 4–8 wk; P < 0.01). Mortality-corrected F:G was improved under 30 Hz for 8 to 11 wk and 0 to 11 wk (P = 0.05 and P = 0.04, respectively). Total mortality was lower under 195 Hz compared to 30 Hz (P = 0.02). Uniformity, gait score, feather condition, and litter quality were unaffected by flicker. Footpad scores were improved under 90 Hz (P = 0.01), leading to an improved average footpad score (P = 0.02). Feather cleanliness was improved under 90 Hz compared to both 30 Hz and 195 Hz (P

A low-protein (LP) diet may alleviate the environmental impact of chicken meat production by reducing nitrogen excretion and ammonia emissions. Thus, this study investigated the effect of a 15% reduced protein diet with or without amino acid (AA) supplementation on the growth performance of broiler chicks from 10 to 35 days of age and the underlying mechanism for loss of skeletal muscle mass. Thirty-six male broiler chicks were allocated to three experimental groups based on body weight: control, LP, and essential AA-supplemented LP (LP+AA). The body weight gain, feed conversion ratio, and weight of breast muscles and legs significantly decreased only in the LP group at the end of the feeding period. Plasma uric acid levels were significantly lower in the LP+AA group than those of the other groups. In the LP group, mRNA levels of microtubule-associated protein 1 light chain 3 isoform B were significantly higher in the pectoralis major, whereas those of atrogin-1, muscle RING-finger protein-1, and myoblast determination protein 1 were significantly higher in the biceps femoris compared to those in the control group. There were no significant differences in insulin-like growth factor 1 mRNA levels in the liver or skeletal muscle between groups. These findings suggested that supplementation with essential AAs ameliorated the impaired effects of an LP diet on growth performance in broiler chicks, and that the transcriptional changes in proteolytic genes in skeletal muscles might be related to the impaired effects of the LP diet.

ABSTRACT: The primary aim of this study was to explore the impact of dietary supplementation with a postbiotic derived from Bacillus subtilis ACCC 11025 on growth performance, meat yield, meat quality, excreta bacterial populations, and excreta ammonia emissions of broiler chicks. A total of 480 day-old Arbor Acre broiler chicks, initially weighing 52.83 ± 1.38 g, were randomly allocated into 4 distinct groups. Each group was housed in 6 separate cages, each containing 20 birds. The experimental phase spanned 42 d, divided into 2 periods (d 1–21 and d 22–42). Dietary interventions were based on a basal diet, with postbiotic supplementation at levels of 0.000, 0.015, 0.030, or 0.045%. Our findings indicate that dietary supplementation with postbiotic had a positive influence on body weight gain (BWG) and feed efficiency. The most substantial improvements in BWG and feed efficiency were observed in the group of broiler chicks fed a diet containing 0.015% postbiotic. Furthermore, the inclusion of postbiotic in the diet led to an increase in the yield of breast and leg muscles, with a significant difference in meat yields observed between the control group and the group receiving 0.015% postbiotic supplementation. It's noteworthy that dietary manipulation did not exert any discernible impact on the quality of breast and leg muscle samples. Concurrently, we observed an elevation in serum albumin and total protein contents corresponding to the increasing postbiotic dosage in the diet. Additionally, dietary supplementation with postbiotic effectively controlled the emission of ammonia from excreta and reduced the abundance of Salmonella in excreta while enhancing the presence of Lactobacillus bacteria. The group receiving 0.015% postbiotic supplementation displayed the lowest levels of ammonia emission and the highest counts of Lactobacillus bacteria in excreta. In light of these results, we conclude that dietary supplementation with 0.015% postbiotic represents an efficacious strategy for increasing BWG and meat yield of broiler chicks by enhancing feed efficiency as well as mitigating ammonia emissions from excreta by modulating the composition of excreta bacterial communities.

Residual feed intake (RFI), a widespread index used to measure animal feed efficiency, is influenced by various individual biological factors related to inter-animal variation that need to be assessed. Herein, 30 Simmental bulls, raised under the same farm conditions, were divided on the basis of RFI values into a high efficient group (HE, RFI = − 1.18 ± 0.33 kg DM/d, n = 15) and a low efficient group (LE, RFI = 0.92 ± 0.35 kg DM/d, n = 15). Subsequently, bulls were slaughtered at an average BW of 734 ± 39.4 kg. Their ruminal fermentation traits were analysed immediately after slaughtering and after 24 h of in vitro incubation. Furthermore, ruminal micro-biota composition and ruminal papillae morphology were examined. The LE group exhibited a higher propionate concentration as a percentage of total volatile fatty acids (17.3 vs 16.1%, P = 0.04) in the rumen fluid collected during slaughtering, which was also confirmed after in vitro fermentation (16.6 vs 15.4% respectively for LE and HE, P = 0.01). This phenomenon resulted in a significant alteration in the acetate−to−propionate ratio (A:P) with higher values for the HE group, both after slaughter (4.01 vs 3.66, P = 0.02) and after in vitro incubation (3.78 vs 3.66, P = 0.02). Methane production was similar in both groups either as absolute production (227 vs 218 mL for HE and LE, respectively) or expressed as a percentage of total gas (approximately 22%). Even if significant differences (P

Feed efficiency is a major production trait in animal genetic breeding schemes. To further investigate the genetic control of feed efficiency in rabbits, we performed a genome-wide association study (GWAS) for growth and feed efficiency on 679 rabbits genotyped with the Affimetrix Axiom Rabbit 200K Genotyping Array. After quality control, 127 847 single-nucleotide polymorphisms (SNP) were retained for association analyses. The GWAS were performed using GEMMA software, applying a mixed univariate animal model with a linear regression on each SNP allele. The traits analysed were weight at weaning and at 63 days of age, average daily gain, total individual feed intake, feed conversion ratio and residual feed intake. No significant SNP was found for growth traits or feed intake. Fifteen genome-wide significant SNPs were detected for feed conversion ratio on OCU7, spanning from 124.8 Mbp to 126.3 Mbp, plus two isolated SNP on OCU2 (77.3 Mbp) and OCU8 (16.5 Mbp). For residual feed intake, a region on OCU18 (46.1-53.0 Mbp) was detected, which contained a putative functional candidate gene, GOT1.

Background and Aim: Improving the feed efficiency of beef cattle is necessary to increase the profitability of meat production. Implementing marker-assisted selection breeding systems can improve the genetic potential of beef cattle for increased productivity. This research aimed to study the effects of insulin-like growth factor (IGF)-1 C472T, growth hormone (GH) C2141G, and GH receptor (GHR) T914A polymorphisms on growth performance and feed efficiency in young Kazakh white-headed cattle. Materials and Methods: Young Kazakh white-headed cattle (n = 50) were grouped after weaning according to sex (28 bulls and 22 heifers) and they were genotyped according to the IGF-1 C472T, GH C2141G, and GHR T914A polymorphisms. The test period was conducted from 8 to 15 months of age. The experimental animals were evaluated for live weight (LW) at the beginning and end of the test period. They were also assessed for average daily gain, hip height, metabolic mid-weight (MMWT), actual dry matter intake (DMI), and residual feed intake (RFI). Results: Significant differences in MMWT were found between the bulls with the IGF-1TT and IGF-1CT genotypes, which was a 2.2 kg increase in heterozygous cattle (p < 0.05). Heterozygous IGF-1CT bulls differed with a higher DMI of 0.087 kg/ day (p < 0.05) compared to IGF-1TT homozygotes. Carriers of the IGF-1TT genotype had the greatest feed efficiency at 0.068 kg/day (p < 0.05). Heifers with the GHCC genotype differed in their maximum DMI with an increase of 1.17%–1.57% (p < 0.05) relative to the other genotypes. The G allele in the GH C2141G polymorphism was associated with better (p < 0.05) feed efficiency in the Kazakh white-headed breed. The minimum DMI and RFI in GHR T914A heterozygous heifers were significantly inferior (p < 0.05) to the other genotypes. Conclusion: Association studies of the IGF-1 C472T, GH C2141G, and GHR T914A polymorphisms indicate a relationship between growth, development, and feed efficiency with the genetic characteristics of young Kazakh white-headed cattle. A significant (p < 0.05) dominant effect was found in the IGF-1 gene in bulls and in the GHR gene in heifers, which should be considered when breeding with heterogeneous parental pairs. The negative effect of the allele substitution in the IGF-1 C472T polymorphism was observed in the LW of heifers (−3.25 kg) at the age of 8 months and bulls (−6.05 kg) at 15 months. The substitution in the GH C2141G polymorphism was associated with a significant reduction in DMI by 0.036 kg (p < 0.05) and an increase in feed efficiency by 0.023 kg (p < 0.05) during the rearing of heifers. These results can improve the production efficiency of mature herds of Kazakh white-headed cattle.

The environmental impact of dairy production can be reduced in several ways, including increasing feed efficiency and reducing methane (CH4) emissions. There is no consensus on their relationship. This study aimed at estimating the correlations between residual feed intake (RFI) and CH4 emissions expressed in g/d methane production (MeP), g/kg of fat- and protein-corrected milk methane intensity (MeI), or g/kg of DM intake methane yield (MeY) throughout lactation. We collected CH4 data using GreenFeed devices from 107 Holstein cows, as well as production and intake phenotypes. RFI was predicted from DM intake, fat- and protein-corrected milk, BW, and body condition score. Five-trait random regression models were used to estimate the individual variance components of the CH4 and production traits, which were used to calculate the correlations between RFI and CH4 traits throughout lactation. We found positive correlations of RFI with MeP and MeI ranging from 0.05 to 0.47 throughout the lactation. Correlations between RFI and MeY are low and vary from positive to negative, ranging from −0.18 to 0.17. Both MeP and MeI are favorably correlated with RFI, as is MeY during the first half of lactation. These correlations are mostly favorable for genetic selection, but the confirmation of these results is needed with genetic correlations over a larger dataset.

ABSTRACT The objective was to evaluate the supplementation of fat-soluble and water-soluble vitamins on the performance and plasma concentrations of vitamins in modern hybrids pigs. A total of 144 commercial hybrid castrated male, 43.531 ± 1.099kg, were used in a randomized block design with six treatments, eight replicates and three animals per pen. The experimental treatments were different vitamin supplementation levels - 0, 25, 50, 75, 100, and 125% of the recommended by Rostagno et al. (2011) for male pigs in growth I (40 to 50kg), growth II (50 to 70kg), and finishing I (70 to 90kg) phases. For growth phases I and II, a linear effect (P

Feed efficiency is one of the most important issues for sustainable pig production. Daily-phase feeding (DPF) is a form of precision feeding that could improve feed efficiency in pigs. Gut microbiota can regulate host nutrient digestion, absorption, and metabolism. However, which key microbes may play a vital role in improving the feed efficiency during DPF remains unclear. In the present study, we used a DPF program compared to a three-phase feeding (TPF) program in growing-finishing pigs to investigate the effects of gut microbiota on feed efficiency. A total of 204 Landrace × Yorkshire pigs (75 d) were randomly assigned into 2 treatments. Each treatment was replicated 8 times with 13 to 15 pigs per replicate pen. Pigs in the TPF group were fed with a commercial feeding program that supplied fixed feed for phases I, II, and III, starting at 81, 101, and 132 d of age, respectively, and pigs in the DPF group were fed a blend of adjacent phase feed from 81 to 155 d at a gradual daily ratio and phase III feed from 155 to 180 d of age. Daily feed intake and body weight were recorded by a computerized device in the feeders. Feces and blood samples were collected from 1 pig per replicate at 155 and 180 d of age. The results showed that the DPF program remarkably improved the feed efficiency at 155 d (P

Background: Farm profitability depends on raising healthy calves, which rests on successful feeding and management programs. Objective: To determine the effects of weaning weight on growth performance, feed efficiency, behavioral traits, as well as feeding cost of Holstein-Friesian and Brown Swiss calves. Methods: Sixty-seven newborn dairy calves were allocated into four weaning-weight groups [60 kg (n=18), 65 kg (n=19), 70 kg (n=18) and 75 kg (n=12)]. Body weights (BW) and measurements were recorded at birth, weaning, and four months of age. An instantaneous sampling method was used to collect behavioral data. Results: Daily weight gain of Brown Swiss calves was higher than Holsteins between weaning and four months of age (p

This study investigated the effects of the dietary addition of wormwood (Artemisia montana Pampan) on growth performance, blood characteristics, carcass traits, and meat quality in growing-fattening pigs. One hundred eighty crossed pigs (Landrace × Yorkshire × Duroc), weighing approximately 70 kg, were divided into four groups of 15 head (eight barrows and seven gilts) per pen, and the experiment was replicated thrice. The basal diet (C) was supplemented with 0.5% (T1), 1.0% (T2), and 1.5% (T3) of dried powdered wormwood, and the pigs were fed an experimental diet for six weeks. The average daily weight gain and feed efficiency were significantly higher (P < 0.05) in treatments than in C. On the contrary, the total cholesterol concentration was significantly lower (P < 0.05) in treatments than in C. Additionally, the high-density lipoprotein cholesterol concentration was significantly higher (P < 0.05), and the carcass grade was better (P < 0.05) in the treatments than in C. The ether extract content of the longissimus dorsi (LD) muscle was lower (P < 0.05) in T2 and T3 than that in C. In contrast, the unsaturated fatty acid (USFA) composition in LD muscle was higher (P < 0.05) in the treatments than in C. Additionally, the marbling and flavour of cooked meat were better (P < 0.05) in the treatments than in C. The dietary addition of wormwood increased (P < 0.05) lightness and yellowness of the surface meat colour and decreased (P < 0.05) juiciness of the LD sensory evaluation. Therefore, the dietary addition of wormwood improved growth performance, feed efficiency, carcass grade, USFA composition, and oxidation of protein and lipids in the LD muscle of growing-fattening pigs.

An experiment was conducted to evaluate the influence of dietary energy and nutrient density (END) on performance and egg quality in laying hens during the peak stage of the first laying cycle. A total of 360, 26-week-old Hy-Line W-36 laying hens were randomly allocated to a completely randomised design with five treatments, graded levels of dietary END (92–108%), with six replicates and 12 birds each. The diet with 100% END was formulated to meet the requirements recommended in the 2016 strain Management Guide. Experiment lasted for 12 weeks. Laying hens up to 33 weeks of age did not able to adjust feed intake (FI) with diet dilution. By increasing dietary END, hen-day egg production (EP), egg mass (EM), and feed conversion ratio (FCR) linearly improved. As birds got older, their FI capacity improved and birds regulated own FI with dietary END. The effect of dietary END was not significant on egg quality traits. During the 26–29 weeks of age, based on broken‐line regression models, the best balance of egg production performance traits and dietary END were found at 102, 104, and 105% for EW, EP & EM, and FCR, respectively. These values were estimated 5% lower for 30–33 week of age period. It is concluded, laying hens up to 33 weeks of age cannot adjust their FI with diet dilution, the gastrointestinal tract capacity is the main factor affects FI. Hence, a diet with higher energy and nutrients concentration require to achieve the desired production performance. Highlight During the peaking period of laying cycle: Feed consumption increase slowly. Monitor feed consumption and adjust diet energy and nutrients level to actual feed intake.

Livestock farming is directly responsible for greenhouse gas (GHG) emissions, mainly due to enteric fermentation. Feed efficiency in livestock species is generally evaluated through feed conversion ratio (FCR) and residual feed intake (RFI), which are associated to GHG emissions. The aim of this study was to characterise FCR and RFI in relation to body traits, feed intake, feeding behaviour and GHG emissions of Holstein bulls. Data were collected between May 2018 and July 2020 on 111 animals. Pearson correlations between studied traits were estimated on the residuals obtained from a linear mixed model which included the fixed effect of the linear covariate of age of bull on the dependent variable and the random effect of the bull. To assess the effect of RFI and FCR the same linear mixed model was implemented firstly by including the fixed effect of RFI (2 classes) and secondly the fixed effect of FCR (2 classes). Correlations between dry matter intake (DMI) and GHG ranged from 0.25 (CH4) to 0.36 (CO2). The strongest relationship was estimated between feed efficiency traits and DMI (0.86). RFI and FCR showed weak to moderate correlations with GHG (0.12–0.31). Animals belonging to the low classes of RFI and FCR had lower DMI and showed significant reduction of GHG emissions. Results of the present study highlighted significant differences in terms of feed efficiency and GHG emissions among tested animals; further research is needed on their progeny to investigate the genetic background of the same efficiency and emission-related traits. Highlights Feed efficiency and emission of greenhouse gases vary among Holstein bulls. Dry matter intake and feed efficiency traits were strongly positively associated. Animals endowed with greater feed efficiency had lower emissions and feed intake. Results of the present study will be useful to select animals for feed efficiency.

Sixty-six dairy farms (as average, 167.0 ± 106.5 milking cows and milk yield of 31.4 ± 5.1) were visited to identify different nutritional approaches adopted in dairy cows diet formulation. Forage, TMR, faecal, and milk samples from lactating herds were collected and diet composition, in vitro methane production, in vivo nutrient digestibility, faecal fermentation profile and milk yield and milk quality were characterised. A hierarchical cluster analysis was applied and six nutritional approaches in diet formulation corresponding with six different clusters (CL) were identified. The CL discriminated the farm feeding choices in the following nutritional strategies, based on high use of: (i) high moisture corn (HMC) and legume silage, (ii) compound feed, (iii) corn and soy meals, (iv) HMC and soy meal, (v) corn meal and protein compound feeds, or (vi) HMC and protein compound feed strategies, respectively for CL1 to CL6. The milk yield tended (p = .061) to differ among CL. The greatest (>1.60) feed efficiency was calculated for CL1 and 5, whereas the lowest (

A four-week feeding trial was conducted to determine the effect of liquid toxin binder supplementation on the production performance of broiler chickens. A total of 400 seven-day-old Cobb broiler chicks were randomly distributed to four treatments following a Complete Randomized Design (CRD). The treatment groups consisted of the control group, continuous liquid toxin binder supplementation, reduced feed toxin binder inclusion, continuous liquid toxin binder supplementation, and intermittent liquid toxin binder supplementation. Each treatment was replicated four times with 25 chicks per replicate. The feeding trial was conducted from the age of 8th to 35th days of age. The acidifier used was a blend of activated charcoal, selenium, vitamin E, probiotics, prebiotics, mannan oligosaccharides, and bacterial cell wall components. The results showed significant effects (P

A study was conducted to investigate the growth rate and carcass characteristics of lambs under different feeding levels. Twenty one lambs (3 months old) were allocated into 3 treatments of feeding levels, namely 4% of body weight (BW) (T1), 5.5% of BW (T2) and provided ad libitum (T3). The diet contained 13.74% of CP and 61.79% of TDN. The lambs were raised to reach 25 kg BW, before being slaughtered. The parameters observed were dry matter intake (DMI), dry matter digestibility (DMD), body weight gain (BWG), feed conversion ratio (FCR), carcass production, meat-bone ratio (MBR), fat distribution, chemical composition of meat and fatty acids profile. The data obtained were analyzed by analysis of variance and continued by Duncan's multi-range test if there was any significant difference among the treatments. The results showed that the average of DMD was 59.71% (P>0.05). The lambs fed T3 had the highest BWG (203 g/day), the lowest FCR (6.8) and the shortest rearing time (67 days) (P0.05). The lambs of T3 had the heaviest carcass fat (2,698 g), the lightest carcass bone (1,985 g), the highest MBR (3.6), and the highest PUFA to SFA ratio (0.55). It can be concluded that the higher the feeding level resulted in the higher growth rate, the lower feed conversion ratio, higher meat-bone ratio and higher carcass fat production, lower feed conversion ratio and the shorter rearing time; but did not af-fect the meat quality.

Abstract Background Improving feed efficiency (FE) is one of the main objectives in broiler breeding. It is difficult to directly measure FE traits, and breeders hence have been trying to identify biomarkers for the indirect selection and improvement of FE traits. Metabolome is the "bridge" between genome and phenome. The metabolites may potentially account for more of the phenotypic variation and can suitably serve as biomarkers for selecting FE traits. This study aimed to identify plasma metabolite markers for selecting high-FE broilers. A total of 441 birds from Northeast Agricultural University broiler lines divergently selected for abdominal fat content were used to analyze plasma metabolome and estimate the genetic parameters of differentially expressed metabolites. Results The results identified 124 differentially expressed plasma metabolites (P

Background: Weaning is the most stressful event in pig’s life, resulting in postweaning diarrhea and growth retardation. The supplementation of Advanced Digestion Enhancing Protein Plus Technology (ADEPPTTM), which contains iron-binding polypeptides and non-starch polysaccharides, to nursery diets may reduce the occurrence of diarrhea and enhance growth performance of weaning pigs. Objective: To evaluate the effect of ADEPPTTM supplementation on growth performance and fecal score in weaning pigs. Methods: At weaning, a total of 54 weaning pigs (initial body weight: 7.42 ± 0.52 kg) were assigned to 3 treatments in 3 replicates with 6 pigs per pen based on sex, breed, and body weight for a 28-d feeding trial. The pigs were fed corn-soybean meal-based diets containing 0.0, 0.5, and 1.0% of the ADEPPTTM product in 2 phases (d 0-14 and d 15-28 postweaning, respectively). In the first week (d 3-5 postweaning) of the trial, a pig was removed from each pen when diarrhea was observed, housed in a separate pen within treatment, and then treated for 3 days with a 100-ml solution of electrolytes and ADEPPTTM by drenching. Growth performance and fecal score (1=normal to 4=watery diarrhea) were measured. Results: In the feeding trial, there were no significant differences in body weight, average daily gain, and average daily feed intake throughout the overall period. However, a quadratic trend was observed in gain to feed ratio (p=0.09) for d 0-7 postweaning and overall period with increasing ADEPPTTM supplementation levels in which the greatest value was observed in the 0.5% ADEPPTTM level. The fecal score tended to decrease linearly with increasing ADEPPTTM levels during d 0-7 (p=0.11) and 0-14 (p=0.12) postweaning. There was no significant difference on fecal score of diarrheic pigs and average daily gain tended to increase linearly in d 21-28 postweaning (p=0.08) with increasing ADEPPTTM levels. Conclusion: These results indicate that 0.5% ADEPPTTM supplementation has a potential to enhance growth performance of weaning pigs and might be effective to prevent and control postweaning diarrhea.

Introduction: Feed is the main cost part of poultry production. High feed efficiency poultry produce less feed and less excrement per unit weight gain. Therefore, a comprehensive understanding of the biological mechanisms that control feed efficiency is crucial for the development of optimal breeding and selection strategies. The serine biosynthesis pathway is one of the most important pathways in animals with high feed efficiency. The aim of this study was to investigate the expression of PHGDH, PSAT1 and PSPH genes by real-time PCR in Iranian native turkeys with high and low feed efficiencies.Materials and Methods: Iranian native male turkeys (n=500) were reared up to 20 weeks of age under standard production guidelines. Then 75 turkeys were randomly selected and placed in separate cages with free access to water and feed from 20 to 24 weeks. Turkeys were ranked based on feed conversion ratio (FCR) and three turkeys with the highest and three turkeys with lowest feed efficiency were selected as high feed efficiency (HFE) and low feed efficiency (LFE) birds, respectively. After slaughter of turkeys, RNA was extracted from breast tissue. Quantity and purity of the extracted RNAs were determined using a nanodrop device and its quality was evaluated using 1% agarose gel electrophoresis. Sequences of PSPH, PHGDH, PSAT1 and RSP7 genes were collected from the NCBI database. The primer was designed using Primer Premier version 5 software. All primers were synthesized by Sinaclon (Iran). In this study, RSP7 gene was used as a reference gene. Then, cDNA synthesis was performed. The best amplification temperature for simultaneous amplification of target and reference genes was determined. Samples were amplified for each gene with 3 replications using real-time PCR reaction. Significance level between treatments for each gene was determined separately using t-test in SAS software version 9.2 (P

The collective economic and environmental interest of the whole dairy sheep sector is to reduce feed costs and the negative impact of milk production on the environment. Thus, this study focused on the characterisation and genetic selection potential of feed efficiency in the Lacaune breed. Estimates for feed efficiency in dairy ewes are limited, mainly due to a lack of individual feed intake measurements in the sheepfold or in the pasture. We estimated the genetic parameters for two approximated (not entirely based on individual data) feed efficiency traits (lactation feed conversion ratio (LFCR) and residual energy intake (REI)) and daily milk yield (DMY) at different stages of lactation and throughout lactation. The accuracy of the efficiency traits was first evaluated on samples from Lacaune dairy ewes that were monitored individually, especially for their feed intake. Then, feed efficiency estimation methods were applied on eight commercial farms corresponding to 4 680 Lacaune dairy ewes over two milk lactations (30 854 records). Animals were collectively (for a large part of feed intake) or individually (for milk performance and dynamics of body fat reserves) monitored at different lactation stages. The heritabilities of LFCR and REI were estimated over lactations at 0.10 ± 0.01 and 0.11 ± 0.01, respectively. High genetic correlations were observed between the two efficiency traits and milk production traits, with a genetic correlation between LFCR and DMY of 0.74 ± 0.04 and between REI and DMY of −0.79 ± 0.04. A strong influence of environmental factors such as farm, year of milk production and lactation stage affected the genetic link between REI and milk production traits. Efficiency values observed in early lactation when animals were bred in the sheepfold were less genetically correlated with values obtained later in lactation when animals were grass-fed. However, individual characterisation of feed efficiency remains difficult due to the collective feeding context in dairy ewe farms.

ABSTRACT: The Korat chicken (KR) is a slow-growing Thai chicken breed with relatively poor feed efficiency (FE) but very tasty meat with high protein and low fat contents, and a unique texture. To enhance the competitiveness of KR, its FE should be improved. However, selecting for FE has an unknown effect on meat characteristics. Thus, understanding the genetic basis underlying FE traits and meat characteristics is needed. In this study, 75 male KR birds were raised up to 10 wk of age. For each bird, the feed conversion ratio (FCR), residual feed intake (RFI), and physicochemical properties, flavor precursors, and biological compounds in the thigh meat were evaluated. At 10 wk of age, thigh muscle samples from 6 birds (3 with high FCR and 3 with low FCR values) were selected, and their proteomes were investigated using a label-free proteomic method. Weighted gene coexpression network analysis (WGCNA) was used to screen the key protein modules and pathways. The WGCNA results revealed that FE and meat characteristics significantly correlated with the same protein module. However, the correlation was unfavorable; improving FE may result in a decrease in meat quality through the alteration in biological processes including glycolysis/gluconeogenesis, metabolic pathway, carbon metabolism, biosynthesis of amino acids, pyruvate metabolism, and protein processing in the endoplasmic reticulum. The hub proteins of the significant module (TNNT1, TNNT3, TNNI2, TNNC2, MYLPF, MYH10, GADPH, PGK1, LDHA, and GPI) were also identified to be associated with energy metabolism, and muscle growth and development. Given that the same proteins and pathways are present in FE and meat characteristics but in opposite directions, selection practices for KR should simultaneously consider both trait groups to maintain the high meat quality of KR while improving FE.

The food security of a country can be harmed if the supply and demand of poultry products are affected due to any disruptions disrupted by factors such as poor growth rates and disease outbreaks. On top of that, with regard to food safety reasons, developed countries have started to prohibit the inclusion of antibiotics in livestock feed. As a result, it is essential to seek alternative preventive substitutes for growth promotion and prophylaxis in poultry rather than depending on antibiotics. Plant extracts are one of the alternatives with anti-microbial and growth-promoting effects on poultry. Saponins have many biological and pharmacological activities like anti-carcinogenic, anti-microbial, anti-parasitic, anti-fungal, anti-inflammatory, hypocholesterolemic, immunomodulatory, and anti-oxidant properties. Because of its growth-promoting effect, immunity enhancer, and microbial capabilities, saponins from diverse sources and quantities could be used as an anti-microbial alternative to synthetic antibiotics in the broiler industry. Additionally, steroidal saponins have been commercialized as supplements for poultry, health foods for human consumption, and potential anti-cancer treatments. Therefore, the present review will critically shed light on the availability of different saponins and the influence on the production performance and immunopathology responses of poultry supplemented with different saponins extract. Information from this review will added knowledge on the usability of different saponins extracts which could be considered as a novel feed additive in the poultry industry.

Feed efficiency is one of the economically important traits for the cattle industry that affects profit (feed costs) and the environment (production of manure and methane). Due to that feed efficiency is driven by multi-factors, mechanisms contributing to the animal to animal variation in this trait have not been well defined, limiting the development of precision feeding strategies to improve the herd production efficiency. Rumen microbial fermentation and volatile fatty acids (VFA) production have been recently reported to be associated with cattle feed efficiency, however the roles of rumen epithelial function in feed efficiency are less studied although the rumen epithelium has an important function in VFA absorption and metabolism which can affect host feed efficiency. Rumen epithelium is colonized with a diverse microbial population, termed epimural microbiota, which has proposed functions in tissue development, barrier and inflammation, urea transport, and oxygen scavenging, suggesting that they can affect rumen epithelial functions and subsequently cattle feed efficiency. Especially, prospective functions of epimural microbiota, enhanced rumen immunity and increased rumen epithelial thickness, might contribute to less nutritional requirement for tissue recuperation. Thus, the understanding of the functions of rumen epithelium, epimural microbiota, and rumen epithelial host-microbe interactions is essential to identify their roles in contributing to feed efficiency. In this review, we will focus on to date research findings on the structure of rumen epithelium, epimural microbiota, and epithelial host-microbe interactions together with their functions and how these are associated with feed efficiency, aiming to provide insights on future directions to study rumen epithelial host-microbe interactions and improve the rumen functions in cattle.

Interest on methane emissions from livestock has increased in later years as it is an anthropogenic greenhouse gas with an important warming potential. The rumen microbiota has a large influence on the production of enteric methane. Animals harbour a second genome consisting of microbes, collectively referred to as the “microbiome”. The rumen microbial community plays an important role in feed digestion, feed efficiency, methane emission and health status. This review recaps the current knowledge on the genetic control that the cow exerts on the rumen microbiota composition. Heritability estimates for the rumen microbiota composition range between 0.05 and 0.40 in the literature, depending on the taxonomical group or microbial gene function. Variables depicting microbial diversity or aggregating microbial information are also heritable within the same range. This study includes a genome-wide association analysis on the microbiota composition, considering the relative abundance of some microbial taxa previously associated to enteric methane in dairy cattle (Archaea, Dialister, Entodinium, Eukaryota, Lentisphaerae, Methanobrevibacter, Neocallimastix, Prevotella and Stentor). Host genomic regions associated with the relative abundance of these microbial taxa were identified after Benjamini-Hoschberg correction (Padj

ABSTRACT The objective of this study was to evaluate characteristics of the testicular parenchyma and vascular parameters of the pampiniform plexus obtained by ultrasound, semen quality parameters, and sperm freezability in Nellore bulls classified based on residual feed intake (RFI). Twenty-seven bulls (21.82±0.88 months of age) evaluated for feed efficiency were sampled for the study, including 15 with low RFI (−0.592±0.09 kg dry matter/day) and 12 with high RFI (0.792±0.10 kg dry matter/day). In ultrasound and Doppler assessment, the most efficient animals (low RFI) showed higher pulsatility and resistive indexes, as well as a tendency towards greater heterogeneity of the testicular parenchyma (0.625±0.032 vs. 0.508±0.032, 1.012±0.072 vs. 0.802±0.072, and 12.9±0.96 vs. 10.2±0.96, respectively, for low vs. high RFI). However, these animals tended to have lower peak diastolic velocity (5.19±0.50 for low RFI vs. 6.54±0.50 for high RFI). Analysis of fresh semen showed a lower percentage of minor defects in low RFI animals (2.67±1.19%) compared with high RFI animals (8.10±1.19%), without differences in the other parameters in fresh or thawed semen and after thermoresistance testing. Evaluation of flow cytometry parameters showed a higher quality of mitochondrial respiration in semen samples of low RFI animals (22.04±2.50%) compared with high RFI animals (12.29±2.71%). Therefore, although RFI exerts an effect on the Doppler parameters of the pampiniform plexus, it is not sufficient to affect the quality of fresh or thawed semen.

ABSTRACT: Improving feed efficiency is an important target for poultry breeding. Feed efficiency is affected by host genetics and the gut microbiota, but many of the mechanisms remain elusive in laying hens, especially in the late laying period. In this study, we measured feed intake, body weight, and egg mass of 714 hens from a pedigreed line from 69 to 72 wk of age and calculated the residual feed intake (RFI) and feed conversion ratio (FCR). In addition, fecal samples were also collected for 16S ribosomal RNA gene sequencing (V4 region). Genetic analysis was then conducted in DMU packages by using AI-REML with animal model. Moderate heritability estimates for FCR (h2 = 0.31) and RFI (h2 = 0.52) were observed, suggesting that proper selection programs can directly improve feed efficiency. Genetically, RFI was less correlated with body weight and egg mass than that of FCR. The phenotypic variance explained by gut microbial variance is defined as the microbiability (m2). The microbiability estimates for FCR (m2 = 0.03) and RFI (m2 = 0.16) suggested the gut microbiota was also involved in the regulation of feed efficiency. In addition, our results showed that the effect of host genetics on fecal microbiota was minor in three aspects: 1) microbial diversity indexes had low heritability estimates, and genera with heritability estimates more than 0.1 accounted for only 1.07% of the tested fecal microbiota; 2) the genetic relationship correlations between host genetics and different microbial distance were very weak, ranging from −0.0057 to −0.0003; 3) the microbial distance between different kinships showed no significant difference. Since the RFI has the highest microbiability, we further screened out three genera, including Anaerosporobacter, Candidatus Stoquefichus, and Fournierella, which were negatively correlated with RFI and played positive roles in improving the feed efficiency. These findings contribute to a great understanding of the genetic background and microbial influences on feed efficiency.

Feed cost is the main factor affecting the economic benefits of pig industry. Improving the feed efficiency (FE) can reduce the feed cost and improve the economic benefits of pig breeding enterprises. Liver is a complex metabolic organ which affects the distribution of nutrients and regulates the efficiency of energy conversion from nutrients to muscle or fat, thereby affecting feed efficiency. MicroRNAs (miRNAs) are small non-coding RNAs that can regulate feed efficiency through the modulation of gene expression at the post-transcriptional level. In this study, we analyzed miRNA profiling of liver tissues in High-FE and Low-FE pigs for the purpose of identifying key miRNAs related to feed efficiency. A total 212~221 annotated porcine miRNAs and 136~281 novel miRNAs were identified in the pig liver. Among them, 188 annotated miRNAs were co-expressed in High-FE and Low-FE pigs. The 14 miRNAs were significantly differentially expressed (DE) in the livers of high-FE pigs and low-FE pigs, of which 5 were downregulated and 9 were upregulated. Kyoto Encyclopedia of Genes and Genomes analysis of liver DE miRNAs in high-FE pigs and low-FE pigs indicated that the target genes of DE miRNAs were significantly enriched in insulin signaling pathway, Gonadotropin-releasing hormone signaling pathway, and mammalian target of rapamycin signaling pathway. To verify the reliability of sequencing results, 5 DE miRNAs were randomly selected for quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR results of miRNAs were confirmed to be consistent with sequencing data. DE miRNA data indicated that liver-specific miRNAs synergistically acted with mRNAs to improve feed efficiency. The liver miRNAs expression analysis revealed the metabolic pathways by which the liver miRNAs regulate pig feed efficiency.

We aimed to investigate candidate proteins related to long-term caloric restriction and feed efficiency in bovine longissimus dorsi muscle (LM). A total of 31 Korean native steers were randomly distributed to ad libitum (n = 16) or caloric restriction group (n = 15) to conduct two feeding trials for 13 mon. In the first trial (10–18 mon of age), steers were fed with 100% ad libitum (NEg = 0.63 Mcal/kg) or caloric restriction (80% of the previous day’s feed intake of ad libitum group). In the second trial (18–23 mon of age), the energy value of 100% ad libitum diet was 1.13 Mcal/kg NEg and those in caloric restriction group diet was 0.72 Mcal/kg NEg. At the endpoint of this experiment, in each group, 6 animals were selected with high (n = 3) or low feed efficiency (n = 3) to collect muscle tissue samples (6 animals/group). From muscle tissues of 23 mo of age, we excavated 9 and 12 differentially expressed (two-fold or more) proteins in a nutritional group and feed efficiency group using two-dimensional electrophoresis, respectively. Of these proteins, heat shock protein beta-6 was up-regulated in both the caloric restriction and the low feed efficiency group. In bovine embryonic fibroblasts, the mRNA expression of heat shock protein beta-6 increased after adipogenic differentiation, however, decreased after myogenic differentiation. Our data provide that heat shock protein beta-6 may be an adipogenic protein involved in the mechanism of caloric restriction and feed efficiency in the LM of the steer.

ABSTRACT The use of blood metabolites (BM), fecal starch (FS), and apparent digestion of starch, (ATTSD) as indicators of feed efficiency (FE) in beef cattle in the feedlot was studied. Fourteen bulls were used, originating in an industrial cross, without a defined racial group, with mean body weight of 284.86kg, individually fed, being evaluated in a 42-day confinement system. After the evaluation, the animals were divided into two groups according to the individual FE: high feed efficiency (HE) and low feed efficiency (LE). There was a difference between the groups in the variables FE, feed conversion (FC), final weight (FW), and daily weight gain (DWG). The FE had a positive correlation with DWG, FC, and FW. There was no difference between the groups for the variables BM, FS, and ATTSD, nor was there any correlation between these variables and FE. Considering the feed cost, the HE animals proved more profitable. BM, FS, and ATTSD did not statistically show potential to be used as indicators of FE, despite the evidence of numerical differences of these variables between the different groups, tendency of correlations with FE, and discriminating function with potential assertiveness.

ABSTRACT: The effect of commercial selection by poultry breeders on the growth, efficiency, and sexual maturity of broiler breeders was studied using 2 University of Alberta Meat Control strains unselected since 1957 and 1978 (AMC-1957 and AMC-1978, respectively) and 2 strains originating from the University of Arkansas; 1995 Random-bred (1995RB) and 2015 Random-bred (2015RB). A study with a 4 × 2 factorial arrangement was conducted with the 4 strains fed at either ad libitum, or restricted levels to achieve a current commercial breeder target BW profile. Growth rate, feed intake, feed efficiency, age at sexual maturity, carcass components, and body conformation were measured. To assess reproductive development, birds were assigned to 2 fates: dissected at photostimulation or dissected after the second oviposition. At 22.4 wk of age, the restricted-fed AMC-1957, AMC-1978, 1995RB, and 2015RB reached 100, 61, 46, and 38% of their ad libitum-fed counterparts’ BW, respectively. During the rearing phase, the amount of feed needed to maintain restricted-fed birds on the target BW was 99.4, 57, 29.5, and 24.9% of their ad libitum-fed counterparts for AMC-1957, AMC-1978, 1995RB, and 2015RB, respectively. Feed restricted birds in the 2015RB had lower heat production relative to the AMC-1957 and AMC-1978, which shows that modern strains utilized feed more efficiently compared to the antique strains. This might be related to the increasing severity of feed restriction of broiler breeders over the past 60 years. Relative to AMC-1957 and AMC-1978 strains, the 1995RB and 2015RB strains had heavier breast muscle and lower fat pad weight. Although the pubertal threshold for age and BW have increased over the last 6 decades, changes in selection programs for feed efficiency have resulted in broiler breeders that prioritize nutrient allocation to growth and breast development rather than adipose storage. As a result, feed restricted modern broiler breeders may have marginally sufficient fat resources to support reproduction.

This study aimed to evaluate feed protein utilization and nitrogen emission of young and mature Kejobong goats fed different concentrations of concentrate and forage. Sixteen heads of male Kejobong goats consisted of eight heads young goats (5 months old) and eight heads mature goats (9 months old) with initial body weight (BW) of 14 ± 1.46 kg, and 22.3 ± 1.99 kg, respectively were arranged in a nested design. All goats were fed with two different rations of concentrate and forage (C30 = 30% concentrate: 70% forage and C70 = 70% concentrate: 30% forage). The data were analyzed using ANOVA procedure. This study showed that the average daily gain (ADG) did not differ (p>0.05) in both ages, but it differed (p0.05), while there was a significantly difference (p0.05). The different ages of goats and concentrate levels affected N retention (g/day) and total N2O emission (g/day). It was concluded that ADG, DCP and FCR did not differ in mature and young Kejobong goats, while young goats had less N2O emissions than mature goats. Goats fed 70% of concentrate improved their ADG, DCP, N retention (g/day) and produced less N2O emission.

Abstract Background Dietary essential oil (EO) supplementation can exert favorable effects on gut health in broilers. However, it is unknown whether EO could improve intestinal functions, consequently beneficial for egg performance and quality in late-phase laying hens. This study was aimed to investigate the potential effects of EO on production performance, egg quality, intestinal health and ileal microbiota of hens in the late phase of production. A total of 288 60-week-old Hy-line Brown laying hens were randomly divided into 4 groups and fed a basal diet (control) or basal diets supplemented with oregano EO at 100, 200 and 400 mg/kg (EO100, EO200 and EO400). Results Dietary EO supplementation resulted in a quadratic decrease (P