1. Bee's honey attenuates non-alcoholic steatohepatitis-induced hepatic injury through the regulation of thioredoxin-interacting protein-NLRP3 inflammasome pathway.
- Author
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Xiao, Jia, Liu, Yingxia, Xing, Feiyue, Leung, Tung, Liong, Emily, and Tipoe, George
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LIVER physiology , *PREVENTION of obesity , *INFLAMMATION prevention , *FATTY liver prevention , *FIBROSIS , *DNA analysis , *GLUCOSE metabolism , *ANIMAL experimentation , *APOPTOSIS , *ASPARTATE aminotransferase , *BIOLOGICAL assay , *BIOLOGICAL models , *ENZYME-linked immunosorbent assay , *FATTY acids , *FAT content of food , *HISTOLOGICAL techniques , *HONEY , *POLYMERASE chain reaction , *PROBABILITY theory , *RATS , *RESEARCH funding , *STAINS & staining (Microscopy) , *STATISTICS , *WESTERN immunoblotting , *MALONDIALDEHYDE , *DATA analysis , *STATISTICAL significance , *OXIDATIVE stress , *ALANINE aminotransferase , *REVERSE transcriptase polymerase chain reaction , *DATA analysis software , *DESCRIPTIVE statistics , *SEQUENCE analysis , *IN vitro studies , *KRUSKAL-Wallis Test , *IN vivo studies , *PREVENTION - Abstract
Purpose: We aim to examine whether honey ameliorates hepatic injury in non-alcoholic steatohepatitis (NASH) animal and cell line steatosis models. Methods: NASH was induced in female Sprague-Dawley rat by 8-week feeding with a high-fat diet. During the experiment, 5 g/kg honey was intragastrically fed daily. Rat normal hepatocyte BRL-3A cell was treated with sodium palmitate (SP) to induce steatosis in the absence or presence of honey pre-treatment or specific siRNA/overexpress plasmid of thioredoxin-interacting protein (TXNIP) or antagonist/agonist of Nod-like receptor protein 3 (NLRP3). Results: Honey significantly improved the high-fat-diet-induced hepatic injury, steatosis, fibrosis, oxidative stress, and inflammation in rats. Honey also inhibited the overexpression of TXNIP and the activation of NLRP3 inflammasome. These effects were replicated in BRL-3A cell line which showed that the down-regulation of TXNIP or inhibition of NLRP3 contributed to the suppression of NLRP3 inflammasome activation, inflammation, and re-balanced lipid metabolism. In contrast, overexpression of TXNIP or agonism of NLRP3 exacerbated the cellular damage induced by SP. Conclusion: Suppression of the TXNIP-NLRP3 inflammasome pathway may partly contribute to the amelioration of hepatic injury during the progression of NASH by honey. Targeting hepatic TXNIP-NLRP3 inflammasome pathway is a potential therapeutic way for the prevention and treatment of NASH. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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