5 results on '"Xiao, Jia"'
Search Results
2. Bee's honey attenuates non-alcoholic steatohepatitis-induced hepatic injury through the regulation of thioredoxin-interacting protein-NLRP3 inflammasome pathway.
- Author
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Xiao, Jia, Liu, Yingxia, Xing, Feiyue, Leung, Tung, Liong, Emily, and Tipoe, George
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LIVER physiology , *PREVENTION of obesity , *INFLAMMATION prevention , *FATTY liver prevention , *FIBROSIS , *DNA analysis , *GLUCOSE metabolism , *ANIMAL experimentation , *APOPTOSIS , *ASPARTATE aminotransferase , *BIOLOGICAL assay , *BIOLOGICAL models , *ENZYME-linked immunosorbent assay , *FATTY acids , *FAT content of food , *HISTOLOGICAL techniques , *HONEY , *POLYMERASE chain reaction , *PROBABILITY theory , *RATS , *RESEARCH funding , *STAINS & staining (Microscopy) , *STATISTICS , *WESTERN immunoblotting , *MALONDIALDEHYDE , *DATA analysis , *STATISTICAL significance , *OXIDATIVE stress , *ALANINE aminotransferase , *REVERSE transcriptase polymerase chain reaction , *DATA analysis software , *DESCRIPTIVE statistics , *SEQUENCE analysis , *IN vitro studies , *KRUSKAL-Wallis Test , *IN vivo studies , *PREVENTION - Abstract
Purpose: We aim to examine whether honey ameliorates hepatic injury in non-alcoholic steatohepatitis (NASH) animal and cell line steatosis models. Methods: NASH was induced in female Sprague-Dawley rat by 8-week feeding with a high-fat diet. During the experiment, 5 g/kg honey was intragastrically fed daily. Rat normal hepatocyte BRL-3A cell was treated with sodium palmitate (SP) to induce steatosis in the absence or presence of honey pre-treatment or specific siRNA/overexpress plasmid of thioredoxin-interacting protein (TXNIP) or antagonist/agonist of Nod-like receptor protein 3 (NLRP3). Results: Honey significantly improved the high-fat-diet-induced hepatic injury, steatosis, fibrosis, oxidative stress, and inflammation in rats. Honey also inhibited the overexpression of TXNIP and the activation of NLRP3 inflammasome. These effects were replicated in BRL-3A cell line which showed that the down-regulation of TXNIP or inhibition of NLRP3 contributed to the suppression of NLRP3 inflammasome activation, inflammation, and re-balanced lipid metabolism. In contrast, overexpression of TXNIP or agonism of NLRP3 exacerbated the cellular damage induced by SP. Conclusion: Suppression of the TXNIP-NLRP3 inflammasome pathway may partly contribute to the amelioration of hepatic injury during the progression of NASH by honey. Targeting hepatic TXNIP-NLRP3 inflammasome pathway is a potential therapeutic way for the prevention and treatment of NASH. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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3. Epigallocatechin gallate attenuates fibrosis, oxidative stress, and inflammation in non-alcoholic fatty liver disease rat model through TGF/SMAD, PI3 K/Akt/FoxO1, and NF-kappa B pathways.
- Author
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Xiao, Jia, Ho, Chi, Liong, Emily, Nanji, Amin, Leung, Tung, Lau, Thomas, Fung, Man, and Tipoe, George
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PROTEIN metabolism , *INFLAMMATION prevention , *FATTY liver prevention , *FIBROSIS , *LIVER analysis , *ANIMAL experimentation , *BIOPHYSICS , *FAT content of food , *HISTOLOGICAL techniques , *INJECTIONS , *RESEARCH methodology , *NECROSIS , *POLYMERASE chain reaction , *POLYPHENOLS , *RATS , *RESEARCH funding , *STAINS & staining (Microscopy) , *STATISTICS , *WESTERN immunoblotting , *GREEN tea , *PLANT extracts , *DATA analysis , *OXIDATIVE stress , *REVERSE transcriptase polymerase chain reaction , *DATA analysis software , *DESCRIPTIVE statistics , *PREVENTION - Abstract
Purpose: To investigate the protective mechanisms of an 85 % pure extract of (−) epigallocatechin gallate (EGCG) in the development of fibrosis, oxidative stress and inflammation in a recently developed dietary-induced animal model of non-alcoholic fatty liver disease (NAFLD). Methods: Female Sprague-Dawley rats were fed with either normal rat diet or high-fat diet for 8 weeks to develop NAFLD. For both treatments, rats were treated with or without EGCG (50 mg/kg, i.p. injection, 3 times per week). At the end, blood and liver tissue samples were obtained for histology, molecular, and biochemical analyses. Results: Non-alcoholic fatty liver disease (NAFLD) rats showed significant amount of fatty infiltration, necrosis, fibrosis, and inflammation. This was accompanied by a significant expressional increase in markers for fibrosis, oxidative stress, and inflammation. TGF/SMAD, PI3 K/Akt/FoxO1, and NF-κB pathways were also activated. Treatment with EGCG improved hepatic histology (decreased number of fatty score, necrosis, and inflammatory foci), reduced liver injury (from ~0.5 to ~0.3 of ALT/AST ratio), attenuated hepatic changes including fibrosis (reduction in Sirius Red and synaptophysin-positive stain) with down-regulation in the expressions of key pathological oxidative (e.g. nitrotyrosine formation) and pro-inflammatory markers (e.g. iNOS, COX-2, and TNF-α). EGCG treatment also counteracted the activity of TGF/SMAD, PI3 K/Akt/FoxO1, and NF-κB pathways. Treatment with EGCG did not affect the healthy rats. Conclusions: Epigallocatechin gallate (EGCG) reduced the severity of liver injury in an experimental model of NAFLD associated with lower concentration of pro-fibrogenic, oxidative stress, and pro-inflammatory mediators partly through modulating the activities of TGF/SMAD, PI3 K/Akt/FoxO1, and NF-κB pathways. Therefore, green tea polyphenols and EGCG are useful supplements in the prevention of NAFLD. [ABSTRACT FROM AUTHOR]
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- 2014
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4. Garlic-derived S-allylmercaptocysteine is a hepato-protective agent in non-alcoholic fatty liver disease in vivo animal model.
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Xiao, Jia, Ching, Yick, Liong, Emily, Nanji, Amin, Fung, Man, and Tipoe, George
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INFLAMMATION prevention , *FATTY liver prevention , *ANIMAL experimentation , *ENZYME-linked immunosorbent assay , *GARLIC , *LIVER , *POLYMERASE chain reaction , *RATS , *RESEARCH funding , *STATISTICS , *WESTERN immunoblotting , *PLANT extracts , *DATA analysis , *OXIDATIVE stress , *REVERSE transcriptase polymerase chain reaction , *CYSTEINE , *DESCRIPTIVE statistics - Abstract
Purpose: To investigate the hepato-protective properties and underlying mechanisms of SAMC in a non-alcoholic fatty liver disease (NAFLD) rat model. Methods: Female rats were fed with a diet comprising highly unsaturated fat diet (30% fish oil) for 8 weeks to develop NAFLD with or without an intraperitoneal injection of 200 mg/kg SAMC three times per week. After euthanasia, blood and liver samples of rats were collected for histological and biochemical analyses. Results: Co-treatment of SAMC attenuated NAFLD-induced liver injury, fat accumulation, collagen formation and free fatty acids (FFAs). At the molecular level, SAMC decreased the lipogenesis marker and restored the lipolysis marker. SAMC also reduced the expression levels of pro-fibrogenic factors and diminished liver oxidative stress partly through the inhibition in the activity of cytochrome P450 2E1-dependent pathway. NAFLD-induced inflammation was also partially mitigated by SAMC treatment via reduction in the pro-inflammatory mediators, chemokines and suppressor of cytokine signaling. The protective effect of SAMC is also shown partly through the restoration of altered phosphorylation status of FFAs-dependent MAP kinase pathways and diminished in the nuclear transcription factors (NF-κB and AP-1) activity during NAFLD development. Conclusions: SAMC is a novel hepato-protective agent against NAFLD caused by abnormal liver functions. Garlic or garlic derivatives could be considered as a potent food supplement in the prevention of fatty liver disease. [ABSTRACT FROM AUTHOR]
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- 2013
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5. S-allylmercaptocysteine reduces carbon tetrachloride-induced hepatic oxidative stress and necroinflammation via nuclear factor kappa B-dependent pathways in mice.
- Author
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Xiao, Jia, Liong, Emily, Ling, Ming-Tat, Ching, Yick-Pang, Fung, Man-Lung, and Tipoe, George
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HEPATOTOXICOLOGY , *LIVER analysis , *ENZYME metabolism , *ANIMAL experimentation , *ANTIOXIDANTS , *BIOPHYSICS , *BLOOD testing , *ENZYME-linked immunosorbent assay , *GARLIC , *HISTOLOGICAL techniques , *INFLAMMATION , *RESEARCH methodology , *LIPID peroxidation (Biology) , *MICE , *NECROSIS , *NITRIC oxide , *POLYMERASE chain reaction , *RESEARCH funding , *STATISTICS , *WESTERN immunoblotting , *PHYTOCHEMICALS , *MALONDIALDEHYDE , *PLANT extracts , *DATA analysis , *OXIDATIVE stress , *ALANINE aminotransferase , *REVERSE transcriptase polymerase chain reaction , *DATA analysis software , *DESCRIPTIVE statistics , *PREVENTION - Abstract
Purpose: To study the protective effects and underlying molecular mechanisms of SAMC on carbon tetrachloride (CCl)-induced acute hepatotoxicity in the mouse model. Methods: Mice were intraperitoneally injected with CCl (50 μl/kg; single dose) to induce acute hepatotoxicity with or without a 2-h pre-treatment of SAMC intraperitoneal injection (200 mg/kg; single dose). After 8 h, the blood serum and liver samples of mice were collected and subjected to measurements of histological and molecular parameters of hepatotoxicity. Results: SAMC reduced CCl-triggered cellular necrosis and inflammation in the liver under histological analysis. Since co-treatment of SAMC and CCl enhanced the expressions of antioxidant enzymes, reduced the nitric oxide (NO)-dependent oxidative stress, and inhibited lipid peroxidation induced by CCl. SAMC played an essential antioxidative role during CCl-induced hepatotoxicity. Administration of SAMC also ameliorated hepatic inflammation induced by CCl via inhibiting the activity of NF-κB subunits p50 and p65, thus reducing the expressions of pro-inflammatory cytokines, mediators, and chemokines, as well as promoting pro-regenerative factors at both transcriptional and translational levels. Conclusions: Our results indicate that SAMC mitigates cellular damage, oxidative stress, and inflammation in CCl-induced acute hepatotoxicity mouse model through regulation of NF-κB. Garlic or garlic derivatives may therefore be a potential food supplement in the prevention of liver damage. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
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