1. Regulation of Macrophage Polarization by miR-449a/Cripto-1-PI3K/AKT/NF-κB Signaling Pathway in Allogeneic Transfusion Mice.
- Author
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Wang, Huan, Yao, Na, Wu, Man-di, Yue, Ke, Bai, Yu, You, Lai-Wei, Liu, Tong, Xu, Fang, and Guo, Jian-Rong
- Subjects
IN vitro studies ,IN vivo studies ,BLOOD transfusion ,ANIMAL experimentation ,WESTERN immunoblotting ,MACROPHAGES ,PROTEOLYTIC enzymes ,CELLULAR signal transduction ,GENE expression ,GENES ,RESEARCH funding ,MICE ,PHOSPHORYLATION - Abstract
In this study, the expression of Cripto-1 and the role of macrophage polarization in immune response after allogeneic transfusion were analyzed by constructing a mouse model of allogeneic transfusion. In order to analyze the effects of miR-449a on the PI3K/AKT/NF-κB signaling pathway and the expression of downstream related regulatory factors under normal and abnormal conditions, we adopt in vitro and in vivo experiments separately. The molecular mechanism of PI3K/AKT/NF-κB signaling pathway was analyzed by blocking or activating gene expression and western blotting. Experiment in vitro has confirmed that inhibition of miR-449a increased the protein expression of Cripto-1. In vivo experiments confirmed that allogeneic transfusion reduced the expression of Cripto-1, which further inhibited NF-κB signaling pathway through AKT/PI3K phosphorylation, regulated macrophage polarization, inhibited M1 polarization of macrophages, promoted M2 polarization, and thus affected immune response of the body. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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