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Your search keyword '"Chen, Yan"' showing total 2 results
Start Over Author "Chen, Yan" Topic animal experimentation Region china
2 results on '"Chen, Yan"'

1. Integration of the blaNDM-1 carbapenemase gene into a novel SXT/R391 integrative and conjugative element in Proteus vulgaris.

Author

Kong, Ling-Han, Xiang, Rong, Wang, Yu-Long, Wu, Shun-Kang, Lei, Chang-Wei, Kang, Zhuang-Zhuang, Chen, Yan-Peng, Ye, Xiao-Lan, Lai, Yan, and Wang, Hong-Ning

Subjects
*CARBAPENEMS, *GENES, *BACTERIAL proteins, *RESEARCH, *PROTEUS (Bacteria), *GENETICS, *ANIMAL experimentation, *RESEARCH methodology, *SWINE, *MEDICAL cooperation, *EVALUATION research, *HYDROLASES, *COMPARATIVE studies
Abstract

Objectives: To characterize the genetic environment of the carbapenem resistance determinant in Proteus vulgaris of swine origin.Methods: The carbapenem-resistant P. vulgaris strain BC22 was isolated from a faecal swab from a diseased pig with diarrhoea in Sichuan Province of China in 2018. The presence of carbapenemase genes was screened by PCR. WGS and bioinformatics analysis were performed to analyse the genetic environment of the carbapenem resistance determinant.Results: P. vulgaris strain BC22 was found to harbour the carbapenemase gene blaNDM-1. WGS data revealed that blaNDM-1 was located in a truncated ISAba125 composite transposon. The carbapenem resistance gene blaNDM-1 and 20 other resistance genes, including the multiresistance gene cfr and the bifunctional aminoglycoside/quinolone resistance gene aac(6')-lb-cr, were located in a novel SXT/R391 integrative and conjugative element (ICE). This new SXT/R391 ICE of 148.7 kb was chromosomally located, and could be transferred to Escherichia coli.Conclusions: Here, we report a carbapenemase gene, blaNDM-1, integrated into an SXT/R391 ICE. Our study highlights that this SXT/R391 ICE may facilitate the dissemination of clinically important resistance genes such as blaNDM-1, cfr and aac(6')-lb-cr. [ABSTRACT FROM AUTHOR]

Published
2020
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2. Dragon's Blood extract has antithrombotic properties, affecting platelet aggregation functions and anticoagulation activities

Author

Xin, Nian, Li, Yu-Juan, Li, Yan, Dai, Rong-Ji, Meng, Wei-Wei, Chen, Yan, Schlappi, Michael, and Deng, Yu-Lin

Subjects
*MEDICINAL plants, *FIBRINOLYTIC agents, *ALTERNATIVE medicine, *ANALYSIS of variance, *ANIMAL experimentation, *BIOPHYSICS, *BLOOD coagulation, *BLOOD platelets, *BLOOD platelet aggregation, *COMPUTER software, *DOSAGE forms of drugs, *HORMONES, *RESEARCH methodology, *MICE, *RATS, *RESEARCH funding, *STATISTICAL hypothesis testing, *T-test (Statistics), *PLANT extracts, *DATA analysis, *PHARMACODYNAMICS
Abstract

Abstract: Ethnopharmacological relevance: Dragon''s Blood from Dracaena cochinchinensis (Lour.) S.C. Chen (Yunnan, China), as a traditional Chinese medicinal herb, was shown to have certain antithrombotic effects. A new preparation process was used to extract effective components from Dragon''s Blood. A 95% ethanol extract A (EA) and a precipitate B (PB) fraction were obtained and compared. Reliability of the preparation process was validated by pharmacodynamic experiments. Materials and methods: A rat/mouse thrombosis and blood stasis model was developed for this study, and EA and PB effects on thrombosis, platelet functions and blood coagulation activities were analyzed. Results: It was observed that the EA fraction had significantly better inhibitory effects than the PB fraction on thrombosis (p <0.05), platelet aggregation function (p <0.01) and anticoagulation activity (p <0.05–0.01). Conclusions: The results obtained here showed that EA fraction from Dragon''s Blood contained pharmacologically effective compounds with antithrombotic effects, partially improving platelet function and anticoagulation activity. [Copyright &y& Elsevier]

Published
2011
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