3 results on '"Yan, Jia"'
Search Results
2. Investigations of the total flavonoids extracted from flowers of Abelmoschus manihot (L.) Medic against α-naphthylisothiocyanate-induced cholestatic liver injury in rats.
- Author
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Yan, Jia-Yin, Ai, Guo, Zhang, Xiao-Jian, Xu, Hai-Jiang, and Huang, Zheng-Ming
- Subjects
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PROTEIN metabolism , *HEPATOTOXICOLOGY , *CHOLESTASIS , *LIVER analysis , *MEDICINAL plants , *ALKALINE phosphatase , *ALTERNATIVE medicine , *ANIMAL experimentation , *ASPARTATE aminotransferase , *BILIRUBIN , *BIOPHYSICS , *ENZYMES , *ESSENTIAL oils , *FLAVONOIDS , *FLOWERS , *GLUTATHIONE , *HISTOLOGICAL techniques , *LACTATE dehydrogenase , *RESEARCH methodology , *NEUTROPHILS , *NITRIC oxide , *ORAL drug administration , *POLYMERASE chain reaction , *RATS , *SUPEROXIDE dismutase , *TUMOR necrosis factors , *WESTERN immunoblotting , *MALONDIALDEHYDE , *ALANINE aminotransferase , *REVERSE transcriptase polymerase chain reaction , *DESCRIPTIVE statistics , *PREVENTION - Abstract
Ethnopharmacology relevance The decoction of the flowers of Abelmoschus manihot (L.) Medic was traditionally used for the treatment of jaundice and various types of chronic and acute hepatitis in Anhui and Jiangsu Provinces of China for hundreds of years. Phytochemical studies have indicated that total flavonoids extracted from flowers of A. manihot (L.) Medic (TFA) were the major constituents of the flowers. Our previous studies have investigated the hepatoprotective effects of the TFA against carbon tetrachloride (CCl 4 ) induced hepatocyte damage in vitro and liver injury in vivo . This study aimed to investigate the protective effects and mechanisms of TFA on α-naphthylisothiocyanate (ANIT)-induced cholestatic liver injury in rats. Material and methods The hepatoprotective activities of TFA (125, 250 and 500 mg/kg) were investigated on ANIT-induced cholestatic liver injury in rats. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were used as indices of hepatic cell damage and measured. Meanwhile, the serum levels of alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), total bilirubin (TBIL), direct bilirubin (DBIL), and total bile acid (TBA) were used as indices of biliary cell damage and cholestasis and evaluated. Hepatic malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione transferase (GST), tumor necrosis factor-α (TNF-α) and nitric oxide (NO) were measured in the liver homogenates. The bile flow in 4 h was estimated and the histopathology of the liver tissue was evaluated. Furthermore, the expression of transporters, bile salt export pump (BSEP), multidrug resistance-associated protein 2 (MRP2), and Na + -taurocholate cotransporting polypeptide (NTCP) were studied by western blot and reverse transcription-quantitative real-time polymerase chain reaction (RT-PCR) to elucidate the protective mechanisms of TFA against ANIT-induced cholestasis. Results The oral administration of TFA to ANIT-treated rats could reduce the increases in serum levels of ALT, AST, LDH, ALP, GGT, TBIL, DBIL and TBA. Decreased bile flow by ANIT was restored with TFA treatment. Concurrent administration of TFA reduced the severity of polymorphonuclear neutrophil infiltration and other histological damages, which were consistent with the serological tests. Hepatic MDA and GSH contents in liver tissue were reduced, while SOD and GST activities, which had been suppressed by ANIT, were elevated in the groups pretreated with TFA. With TFA intervention, levels of TNF-α and NO in liver were decreased. Additionally, TFA was found to increase the expression of liver BSEP, MRP2, and NTCP in both protein and mRNA levels in ANIT-induced liver injury with cholestasis. Conclusion TFA exerted protective effects against ANIT-induced liver injury. The possible mechanisms could be related to anti-oxidative damage, anti-inflammation and regulating the expression of hepatic transporters. It layed the foundation for the further research on the mechanisms of cholestasis as well as the therapeutic effects of A. manihot (L.) Medic for the treatment of jaundice. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
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3. A simple high-performance liquid chromatographic method for the determination of brazilin and its application to a pharmacokinetic study in rats.
- Author
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Yan-yan, Jia, Yan, Li, Ying, Song, Jinyi, Zhao, Fang, Dou, Yuan, Sun, and Ai-dong, Wen
- Subjects
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HIGH performance liquid chromatography , *MEDICINAL plants , *ALTERNATIVE medicine , *ANIMAL experimentation , *BIOPHYSICS , *DOSE-effect relationship in pharmacology , *INTRAVENOUS therapy , *RESEARCH methodology , *RATS , *TIME , *PLANT extracts , *DATA analysis software , *DESCRIPTIVE statistics - Abstract
Abstract: Ethnopharmacological relevance: Caesalpinia sappan is a medicinal plant native to China popularly used to treat chronic pelvic inflammation, dysmenorrhea and hysteromyoma. Its main bioactive component is brazilin which had presented antibacterial, anti-inflammatory and anti-platelet aggregation activities. To establish a sensitive, selective, reproducible, and accurate high performance liquid chromatographic (HPLC) method for the quantitative determination of brazilin in plasma, and study the pharmacokinetics of brazilin in rats after intravenous administration of brazilin. Materials and methods: Rats received intravenous injection of 25, 50 and 100mg/kg of brazilin. Concentrations of brazilin in plasma were determined by HPLC method at different time points and all pharmacokinetic parameters were estimated by non-compartmental analysis with WinNonLin 6.2 software. Results: After single intravenous doses of 25, 50 and 100mg/kg brazilin in rats, the main PK parameters were as follows: C max were 18.1±4.1, 46.7±8.7 and 82.2±9.6µg/mL; AUC0–24 were 20.4±4.3, 48.7±6.8 and 90.4±10.3µgh/mL; and t 1/2 were 5.4±1.5, 5.8±0.9 and 6.2±1.2h, respectively. Conclusion: It showed that the brazilin was eliminated moderately in rat by intravenous injection route with t 1/2 of 6h and showed a dose-dependence profile of C max and AUC0–24 at the doses of 25~100mg/kg of brazilin for injection in rats. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
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