1. Hepatoprotective and Anti-oxidant Activities of Glossogyne tenuifolia Against Acetaminophen-Induced Hepatotoxicity in Mice.
- Author
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Tien, Yu-Hsiu, Chen, Bing-Huei, Wang Hsu, Guoo-Shyng, Lin, Wan-Teng, Huang, Jui-Hua, and Lu, Yi-Fa
- Subjects
HEPATOTOXICOLOGY ,ACETAMINOPHEN ,ANALYSIS of variance ,ANIMAL experimentation ,ANTIOXIDANTS ,GLUTATHIONE ,HIGH performance liquid chromatography ,LIPID peroxidation (Biology) ,MICE ,PHENOLS ,RESEARCH funding ,PLANT extracts ,FREE radical scavengers ,DESCRIPTIVE statistics ,PREVENTION - Abstract
The present study investigated the anti-oxidative and hepatoprotective effects of Glossogyne tenuifolia (GT) Cassini, against acetaminophen-induced acute liver injury in BALB/c mice. The extracts of GT by various solvents (hot water, 50% ethanol and 95% ethanol) were compared for their 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power, total phenolic content, and total anti-oxidant capacity. The results showed that hot water (HW) extracts of GT contained high levels of phenolics and exerted an excellent anti-oxidative capacity; thus, these were used in the animal experiment. The male BALB/c mice were randomly divided into control group, acetaminophen (APAP) group, positive control group and two GT groups at low (GT-L) and high (GT-H) dosages. The results showed that mice treated with GT had significantly decreased serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). GT-H increased glutathione levels and the ratios of reduced glutathione and oxidized glutathione (GSH/GSSG) in the liver, and inhibited serum and lipid peroxidation. This experiment was the first to determine phenolic compounds, chlorogenic acid and luteolin-7-glucoside in HW extract of GT. In conclusion, HW extract of GT may have potential anti-oxidant capacity and show hepatoprotective capacities in APAP-induced liver damaged mice. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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