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The relevance of the subject is the significance of exploring the role of the autonomic nervous system in regulating the cardiovascular system to improve the welfare of productive animals. Heart rate variability is a non-invasive research method that can be useful in exploring the health status of an animal and analysing its psychophysiological state in farm conditions. The purpose of the research -- to determine the effect of the tone of autonomic nervous regulation on the cow's body, which is reflected in changes in the sympathovagal balance. Experimental research was conducted on Ukrainian Black-and-White dairy cows. To explore the variability of heart rate, an electrocardiograph was used, followed by the determination of the main indicators according to the Baevsky method, which included the determination of mode, mode amplitude, variation range, autonomic balance index, autonomic rhythm index and stress index. Based on the results of the study, three experimental groups of animals were established: normotonics, vagotonics, and sympathotonics. Considering the results obtained, cows, depending on the influence of the tone of the autonomic nervous system, have differences in the activity of the cardiovascular system. It will result in different responses to stress, which in turn will affect their productivity. Determination of heart rate variability can be one of the indispensable indicators in analysing the health of an animal on a dairy farm. This issue is a promising area of research, especially when exploring the metabolic processes of high-yield cows to improve productivity while maintaining the physiological state of the animal [ABSTRACT FROM AUTHOR]