1. A Recombinant DNA Plasmid Encoding the sIL-4R-NAP Fusion Protein Suppress Airway Inflammation in an OVA-Induced Mouse Model of Asthma.
- Author
-
Liu, Xin, Fu, Guo, Ji, Zhenyu, Huang, Xiabing, Ding, Cong, Jiang, Hui, Wang, Xiaolong, Du, Mingxuan, Wang, Ting, and Kang, Qiaozhen
- Subjects
RECOMBINANT DNA ,INTERLEUKIN-4 receptors ,ANIMAL models of asthma ,PLASMID genetics ,GENETIC code ,OVALBUMINS ,INFLAMMATION ,AIRWAY (Anatomy) - Abstract
Asthma is a chronic inflammatory airway disease. It was prevalently perceived that Th2 cells played the crucial role in asthma pathogenesis, which has been identified as the important target for anti-asthma therapy. The soluble IL-4 receptor (sIL-4R), which is the decoy receptor for Th2 cytokine IL-4, has been reported to be effective in treating asthma in phase I/II clinical trail. To develop more efficacious anti-asthma agent, we attempt to test whether the Helicobacter pylori neutrophil-activating protein (HP-NAP), a novel TLR2 agonist, would enhance the efficacy of sIL-4R in anti-asthma therapy. In our work, we constructed a pcDNA3.1-sIL-4R-NAP plasmid, named PSN, encoding fusion protein of murine sIL-4R and HP-NAP. PSN significantly inhibited airway inflammation, decreased the serum OVA-specific IgE levels and remodeled the Th1/Th2 balance. Notably, PSN is more effective on anti-asthma therapy comparing with plasmid only expressing sIL-4R. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF