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The purpose of artificial insemination using sexing semen was to obtain the calf according to the expected sex. This study aimed to determine the success of artificial insemination in non-sexing and sexing of frozen semen. The materials used in this research were 77 non-sexing artificial insemination acceptor cows and 77 sexing artificial insemination acceptor cows with double dose treatment. The method used in this study was experimental field experiment with the research treatment: P0 = frozen non-sexing semen straw and P1 = frozen semen of spermatozoa Y by sexing method using Percoll Density Gradient Centrifugation (SGDP) produced by Balai Besar Inseminasi Buatan (BBIB) Singosari. The variables of this study were Non Return Rate (NRR), Service Per Conception (S/C) and Conception Rate (CR). The data obtained were analyzed descriptively. The results of the study in each non-sexing and sexing artificial insemination treatment were: the percentage of NRR 1 were 90.91% and 77.92%, followed by NRR 2 with 88.31% and 76.62%, the pregnancy percentage were 84.42% and 75.33%, and the CR values were 79.22% and 57.14%. The conclusion of this study was artificial insemination using frozen non-sexing semen had a higher success rate compared to artificial insemination using frozen sexing semen.

Kualitas spermatozoa pada semen beku sexing sangat penting dalam menunjang keberhasilan inseminasi buatan untuk mendapatkan pedat dengan jenis kelamin sesuai harapan. Tujuan penelitian ini bertujuan untuk mengetahui kualitas semen beku semen beku non sexing dan sexing secara makroskopis dan mikroskopis. Materi dalam penelitian menggunakan starw semen beku non sexing dan sexing dengan metode pemisahan Sentrifugasi Gradien Densitas Percoll (SGDP) sapi Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Metode penelitian ini menggunakan eksperimental dengan percobaan labolatorium (laboratory experiment ). Variabel penelitian meliputi keadaan umum meliputi tahun produksi straw, secara makroskopis meliputi pH semen beku dan secara mikroskopis meliputi persentase motilitas, viabilitas, abnormalitas, konsentrasi dan total spermatozoa motil. Data yang diperoleh diolah Microsoft Excel dengan analisis Ragam Analysis of variance (Anova) single factor dianalisis secara deskriptif. Hasil penelitian yaitu semen beku non sexing dan sexing meliputi keadaan umum diproduksi tahun 2012 dan 2018, kualitas secara makroskopis memiliki nilai pH sebesar 6,67 dan 6,60 dan kualitas secara mikroskopis memiliki persentase motilitas sebesar 36,00 % dan 31,40 %, viabilitas sebesar 81,70 % dan 75,89 %, abnormalitas sebesar 6,93 % dan 6,78 %, konsentrasi spermatozoa sebesar 31,67 juta/ mini straw dan 12,125 juta/ mini straw dan total spermatozoa motil sebesar 11,39 juta/mini straw dan 5,10125 juta/mini straw. Kesimpulan penelitian bahwa Kualitas post thawing semen beku non sexing lebih tinggi daripada semen beku sexing menggunakan metode SGDP baik secara makroskopis maupun mikroskopis.

Coconut water extender is the solution to the difficulty and high cots of procurement of diluents. The objective of this study was to examine the effect of various formulations of coconut water extender on quality of PO bull sperm during storage in 4-5 o C. This study was carried out at Animal Reproduction Laboratory of Faculty of Animal Husbandry Brawijaya University and Laboratory of Beef Cattle Research Center Grati Pasuruan on March to April, 2018. The study method used laboratory experimental. The experimental design was Factorial Randomized Completely Block Design and the data were analyzed by Analyze of Variance. There are four treatments in this study (P0 = CEP-3 + 20 % of yolk, P1 = Coconut Water + 20% of yolk, P2 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 1 mg/ml, dan P3 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 2 mg/ml) and ten replications of each treatment. There were significant differences in sperm motility between CEP-3 extender and coconut water-based with various formula extenders during chilled storage at 2-5 o C. But the treatment between coconut water-based formulas did not show a significant difference. Coconut water extenders are able to maintain sperm motility up to day 5 with values above 57%.

Sexing spermatozoa bertujuan untuk mengatur jenis kelamin sesuai harapan. Salah satu metode sexing adalah dengan menggunakan Sentrifugasi Gradien Densitas Percoll. Tujuan dari penelitian ini adalah untuk mengetahui berbagai parameter motilitas menggunakan Computer-assisted Sperm Analysis dan proporsi spermatozoa X dan Y menggunakan metode Sentrifugasi Gradien Densitas Percoll dengan volume awal yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong Grati, Pasuruan, pada bulan Januari sampai Maret 2020. Materi yang digunakan adalah Semen Sapi Peranakan Ongole berumur ±5 tahun dan bobot badan ±700 kg sebanyak 3 ekor, motilitas masa ≥ 2+ dan motilitas individu ≥ 70%. Metode yang digunakan adalah eksperimental menggunakan Rancangan Acak Kelompok (RAK) dengan 3 perlakuan volume saat sexing yaitu P1= 1 ml, P2= 1,5 ml dan P3= 2 ml dengan ulangan 11 kali. Ulangan juga berfungsi sebagai kelompok (block). Hasil penelitian menunjukkan bahwa setelah dilakukan sexing dan pendinginan hingga 5oC persentase motilitas adalah P1:71,02+10,08 %; P2: 79,63+8,65 % dan P3: 83,38+6,67 %, sedangkan motilitas progresif pada P1: 47,68+8,71%; P2: 59,61+7,26 % dan P3: 62,21+6,74%. Curvilinear Velocity (VCL) pada P1: 50,9±7,73 µm/s, P2: 55,2±5,03 µm/s dan P3: 53,2±5,97%. Straight-line Velocity (VSL) pada P1: 20,8±8,19 µm/s; P2: 21,6±6,02 µm/s; P3: 22,1±5,77 µm/s, sedangkan Average Path Velocity (VAP) pada P1: 30,2±7,82 µm/s; P2: 32,5±6,14 µm/s dan P3 : 31,5±6,18 µm/s. Linearity (LIN) pada P1: 40,2±12,26%;P2: 39,1±10,31%;P3: 39,8±7,25%. Straightness (STR) pada P1: 67,2±11,20%; P2: 65,7±10,06%;P3: 67,2±7,92% sedangkan Wobble (WOB) pada P1: 59±9,49%; P2: 58,8±8,63% dan P3: 59,1±7,74%. Kesimpulan dari penelitian adalah motilitas dan motilitas progresif spermatozoa pada sampel dengan volume awal 2 ml lebih baik dibandingkan dengan sampel dengan volume awal 1 ml dan 1,5 ml. (Characteristics of Sexing Spermatozoa Motility in Ongole Cattle with Different Initial Volume) ABSTRACT. Sperm sexing is a technique of sorting a specific type of sperm cell to fertilize the egg cell. One of the sexing methods that can be used for spermatozoa sexing is percoll gradient density centrifugation. This research aims to find out various motility parameters using Computer-assisted Sperm Analysis and the proportion of spermatozoa X and Y using density gradient centrifugation method percoll with different initial volume. The study was conducted at Grati Beef Cattle Research Station, Pasuruan, from January to March 2020. Materials of the study were Semen of 3 Filial Ongole Cattles, aged ± 5 years, with ± 700 kg body weight, mass motility ≥ 2+, and individual motility ≥ 70%. The method used was experimental using a Randomized Group Design with 3 treatment volume when sexing i.e. T0= 1 ml, T1= 1.5 ml dan T2= 2 ml with repetition 11 times. The repetition also functions as groups (blocks). The results showed that after sexing and cooling up to 5oC, the percentage of motility is T0:71.02+10.08 %; T1: 79.63+8,65 % and T2: 83.38+6.67 %. Progressive motility on T0: 47.68+8.71%; T1: 59.61+7.26 % and T2: 62.21+6.74%. Curvilinear Velocity (VCL) on T0: 50.9±7.73 µm/s, T1: 55.2±5.03 µm/s and T2: 53.2±5.97%. Straight-line Velocity (VSL) on T0: 20.8±8.19 µm/s; T1: 21.6±6.02 µm/s; T2: 22.1±5.77 µm/s. The Average Path Velocity (VAP) on T0: 30.2±7.82 µm/s; T1: 32.5±6.14 µm/s and T2 : 31.5±6.18 µm/s. Linearity (LIN) on T0: 40.2±12.26%; T1: 39.1±10.31%;T2: 39.8±7.25%. Straightness (STR) on T0: 67.2±11.20%; T1: 65.7±10.06%; T2: 67.2±7.92%. Wobble (WOB) on T0: 59±9.49%; T1: 58.8±8.63% and T2: 59.1±7.74%. The conclusion of the study is the motility and progressive motility of spermatozoa in samples with an initial volume of 2 ml is better than samples with an initial volume of 1 ml and 1.5 ml.

The purpose of this research was to investigate the successful difference of artificial insemination (AI) using frozen semen and liquid semen with CEP-3 + egg yolk diluent of Ongole Crossbred cow indicated by Non Return Rate (NRR) and Conception Rate (CR). The materials used were 54 heads of cow which selected purposively using some criteria such as healthy, free from any reproduction disorder and having at least sexual desire criteria 3A (red, swollen and warm). This field experiment consisted of direct observation and interview to acquire primary and secondary data. The datas were analyzed descriptively and continued by Chi-square test. The results showed that the value of NRR was insignificant influenced between the two treatment. The Conception Rate for frozen semen and liquid semen was found 62.96% and 51.85% respectively. The conclusion of this experiment was the successful rate of artificial insemination (AI) using liquid semen with the CEP-3 + 20% egg yolk diluent was lower than artificial insemination using frozen semen. The value of Conception Rate of frozen semen treatment was 62.96% and the liquid semen treatment was 51.85%.

Although Etawah Crossbred (PE) goat is considered to be dual purpose (meat and milk) goat, it is mainly raised for meat production. Since early 1990, there has been a growing interest of the farmer in some places to raise PE goat for milk production without sacrificing its role to produce kids for meat. Although milk yield of PE goat was not as high as milk yield of some other dairy goats, the ability of PE goat to cope with harsh local environment, particularly climate and feed conditions, was an advantage. Therefore, raising PE goat would still be an important part of farmer activities in the rural areas in Indonesia. Identification of the genes underlying livestock production traits leads to more efficient breeding programs and it is a promising way to improve production traits of farm animals. Growth hormone is a polypeptide hormone which is the major regulator of the metabolic procedures of growth and development and it is encoded by GH gene. The objective of this study was to detect the genetic polymorphism of GH gene in major Etawah Crossbred (PE) goat using PCR-RFLP. The PCR amplified fragment were digested with Hae III endonuclease and the result showed the presence of two genotype CC and CD. The total frequency were 47.0% and 53.0% for CC and CD genotype respectively in 94 tested goats. Statistical analysis showed that in the fragment amplified by the pair of primer, CD genotype had significant higher birth weight and weight of 100 days old (weaning weight) than CC genotype (P

The purpose of this research was to know the effect of castration to Wagyu crossbreed cattle production performance based on age difference and know the appropriate age to do castration. The materials used in this research were 37 heads of 3-month old and 28 heads 4 months calf castration. The method was case studies with purposive sampling. Data were analyzed using unpaired t test. The results showed that the calf castration age of 3 months and 4 months showed significantly different (P

The purposes of this study were determine the carcass characteristics and physical meat quality of Brahman Cross Steer on different rest periods. The experiment was conducted on June 2015 at Slaughter house Cianjur Arta Makmur Ltd. in the Cianjur District, West Java. The total number of cattle used in the study was 30 heads of Brahman Cross Steer treated for 18 hours lairage duration and 30 heads of Brahman Cross Steer treated for 3 hours lairage duration from Pasir Tengah feedloter Ltd (Holding Widodo Makmur Perkasa Ltd). The method used a case study. Data were analyzed using unpaired t test. The results showed that the carcass characteristics including slaughtered weight, hot carcass weight, chilled carcass weight and carcass weight loss percentage were not significantly different, while the dressing percentage for 18 hours lairage duration (54.16±1.62%) and 3 hours lairage duration (55.60±2.00%) was significantly different (P

The aim of this study was to determine the distribution of beef carcass component from Brahman Cross cattle at different slaughtered age. The number of cattle was 99 head (49 steers and 50 heifers), the body weight 350 - 500 kg and age ranged PI0 - PI2 classess. The cattle were raised in PT. Widodo Makmur Perkasa and then slaughtered at slaughter house in Cianjur District, West Java. Accidental sampling method was applied to the research. A direct observation was done to obtain information about age, sex, body weight and carcass weight and its part (meat, bone and fat). The data were analyzed using independent t-test, correlation and linear regresion. The result showed that the average slaughtered weight and carcas weight of Brahman Cross heifers was higher than that of Brahman Cross steers at PI0 sex-class (P0.05). Average meat percentage of heifers and steers was also relatively similar (73.39% : 73.65%). Average bone percentage of steers was higher than that of heifers (14.82% : 14.33 %). Whereas, average fat percentage of heifers was higher than that of steers (11.00% : 10.21%). The research concluded that steers of Brahman Cross cattle at all age-classess had better distribution of carcass component than heifers in the same age-classess. Steers of Brahman Cross cattle at PI2 slaughtered age-class had an optimal distribution of carcass components. Keyword: Brahman Cross, slaughtered age, sex-class, distribution, carcas component.

The purpose of this study was to determine the success of artificial insemination (AI) using liquid sperm Filial Ongole Bulls after sexed by the percoll density gradient centrifugation (PDGC) and egg white sedimentation (EWS) sexed methods. The study materials were 135 cattle were inseminated using non sexed semen, PDGC and EWS sexed methods. Non-return rate (NRR) and the conception rate (CR) are observed. The research design used for the success of AI using chi square. The result of pregnancy examination at the age of 3 months found 21 head of pregnant cows from 45 cattle in AI with without sexed (control), 14 head of pregnant cattle from 45 cattle in the AI with EWS method, 18 head of pregnant cattle from 45 cattle in AI with PDGC method. The result showed that CR value using liquid sperm result of EWS, PDGC and without sexed were very significant (P

The Artificial Insemination (AI) results in Beef cattle are expected to be male calf because they can increaseweight gain rapidly, so in this study AI was carried out by using sexing semen with Percoll density gradient centrifugation method containing Y sperm.This study was conducted to evaluate the success rate of AI using frozen sexing semen on Ongole Cross Breed. The study method used wasexperimental, comparing AI frozen sexing semen and frozen semen in 54 Ongole Cross Breed. The study variableswere Non Return Rate1 (NRR1), Non Return Rate2 (NRR2) and Conception Rate (CR).The study showed that the success rate of based on the value of NRR1 and NRR2AI using frozen sexing semen on Ongole Cross Breed is lower than un sexed frozen semen with the NRR1 value is 73,91%; 93,54% while the value of NRR2 was 69,56%; 83,87%. Based on CR value of frozen sexing semen higher than un sexed frozen semen showed CR valuewas 43,47%; 35,48%.

Artificial Insemination (AI) is a technique that is widely used by farmers to improve the genetic quality of livestock. This method can be increased in value by using sexing semen that can regulate and produce the sex of the offspring as expected. The purpose of this study was to determine the success of AI by using frozen sexing semen on Ongole Crossbred cows in Malang Regency. The materials used were 98 Ongole crossbred cows in traditional farm of Malang Regency especially in Pakis, Tumpang, Jabung and Singosari Districts. The method used was a field experiment with two treatments: 49 cows were inseminated by using frozen sexing semen (P1) and 49 cows were inseminated by using non sexing semen (P0). The variables observed were the Non-Return Rate (NRR) value observedin 3 estrous cycle after AI and CR value based on pregnancy observation at 8 months of pregnancy. Data were analyzed descriptively and continued with a one-proportion z-test. The datashowed that the CR value on P1; P0 were 55.10% and 65, 31% respectively.furthemore, the NRR value on P1 from NRR1 to NRR3 were 87,76%, 73,47% and 67,35%, while on P0 95,92%, 83,67% and 75,51% respectively.It can be conclude thatCR and NRR value of sexing semenwas lower than non sexing semen, however sexing semen can be applied for certain purposes such as producing offspring as expected

Absctract : Sweet Potatoes Leaf - Breast Milk Production. Breast Milk is a good nutrition for baby. Breast milk important for growing babies. To increase breast milk it is important for mother to consume food like: Katu leaf (sauropus androgynus), sweet potatoes leaf (Ipomoe batatas), Kelor Leaf ( moringa oleifera), fread corn etc. The composition of sweet potatoes leaf is low fat and cholesterol. This leaf is source of proteins, calcium, niacin and iron. Sweet potatoe leaf also contain high fibre, pro vitamins A, vitamin C, riboflamin, vitamin B6, folat, magnecium fosfor, potassium and mangan. It increases breast milk because this leaf contains lagtagagum. The goal of this research is to know the relationship between the consumption sweet potatoes leaf wit breast milk production on lactation mother at Independent Midwife Practice Indarwati, Mranggen and Siti Sujalmi, Socokangsi, Jatinom, Klaten. Methods : Pre=Post test with Control Group. Design : this research measure the influence between experiment and control group during September 2014 until February 2015. Population in this research is all breastfeeding mothers. Sample was taken wih quota sampling wit 15 mothers for intervention group and 15 mothers for control group with inclusive criterion is mothers with normal delivery and healthy newborn babies. Univariat analysis performed to describe the variable with the result is 73.3 % intervention group more produced breast milk compared to control group 13.3%. Bivariat analize to know the relationship between independent and dependent variable by using Pearson Correlation with the statistic result p value= 0.000. Conclusion, There was significant relationship between the consumption sweet potatoes leaf for increasing breast milk production. Keyword : Sweet Potatoes Leaf - Breast Milk Production Link OJS 3 artikel HUBUNGAN KONSUMSI EKSTRAK DAUN KENTANG MANIS DENGAN PRODUKSI ASI DI LAKTASI IBU DI KABUPATEN KLATEN

Piglet birth weight has an economic value and commonly correlated with weaning weigh. Therefore, birth weight traits of piglet needs to be selected for genetic improvement. The purpose of a study was to estimate the genetic improvement of piglet birth weight based on heritability and breeding value. Around 154 heads of offsprings generated from 6 sires and 14 sows were used in this study. The data of piglet birth weight was analyzed for heritability (nested classification), breeding value, selection response, and genetic improvement estimation. The result showed that the heritability (h 2 ) of offsprings was 0.24±0,15. Based on breeding value, sires which had high piglet birth weight can be used for male ranking. Selection of response value was 0.06 kg. According to the selection of response value, improvement of birth weight in piglet based on the sire used can be estimated.