Search

Made available in DSpace on 2019-09-12T16:56:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2016 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Universidade Estadual Paulista (Unesp) The systematic knowledge of Simpsonichthys has changed substantially in recent years, with five subgenera having been elevated to the genus category. In view of these taxonomic changes, the aim of the present study was to identify the phylogenetic relationships among this group in order to test a hypothesis of the division of Simpsonichthys. The ATPase 8 and 6 gene sequences of 53 specimens of Simpsonichthys, Xenurolebias, Ophthalmolebias, Spectrolebias and Hypsolebias genera were assessed. The final topology divided the Simpsonichthys subgenera into five clades: clade I (Hypsolebias antenori group and Ophtalmolebias), clade II (Simpsonichtys and Spectrolebias), clade III (Hypsolebias flammeus and H. magnificus groups), clade IV (H. notatus group) and clade V, which included the Xenurolebias genus as a sister group to all the other Simpsonichthys subgenera. Simpsonichthys, Spectrolebias and Hypsolebias could therefor not be described as monophyletic groups, as has been proposed in some hypotheses. The H. antenori, H. notatus and H. magnificus groups, however, were monophyletic. The molecular results also suggested that H. ocellatus belongs to the Hypsolebias flammeus group. The phylogenetic position of H. fasciatus suggested a new group within Hypsolebias, or possibly even a new genus. The characteristics of the Hypsolebias antenori group and their phylogenetic position suggested that only the species of this group should be included in the Hypsolebias genus. The basal position of the Hypsolebias notatus group and its morphological character may represent a new genus. Although the monophyletism of Simpsonichtys could not be recovered, the branches supported the division of the western and eastern clades, possibly due to allopatric speciation. These facts suggest the division of the genus into at least two major clades. In addition, the study of morphological and molecular data is suggested to obtain a better understanding of such complex organisms, and a major taxonomic review is required. [Ponzetto, Josi M.; Parise-Maltempi, Patricia P.; Alves, Anderson L.] Univ Estadual Paulista, Dept Biol, Ave 24A,1515 Bela Vista, BR-13506900 Sao Paulo, Brazil [Ponzetto, Josi M.] Univ Fed Sao Carlos, Dept Genet & Evolut, Rodovia Washington Luis,Km 235,SP 310, BR-13565905 Sao Paulo, Brazil [Britzke, Ricardo] Univ Estadual Paulista, Dept Morphol, Inst Biociencias, Distrito Rubiao Jr S-N, BR-18618970 Sao Paulo, Brazil [Britzke, Ricardo] Univ Tecn Machala, Fac Ciencias Agr, Lab Acuacultura, Km 4-1-2 Via Machala Pasaje,E583 Machala, El Oro, Ecuador [Nielsen, Dalton T. B.] Universidade de Taubaté (Unitau), Dept Biol, Praca Marcelino Monteiro 63, BR-12030010 Sao Paulo, Brazil [Alves, Anderson L.] Embrapa Pesca & Aquicultura, Quadra 104 Sul,Ave 1,34 Conjunto 4, BR-77020020 Palmas, Tocantins, Brazil

Made available in DSpace on 2015-10-21T13:12:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-02-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The species of genus Astyanax is widely distributed in freshwater neotropical zones. Astyanax is considered to be taxonomically confused, similar to other genera placed incertae sedis in Characidae. The cytogenetics of this genus is well characterized; species vary widely in diploid number, from 2n=36 chromosomes in Astyanax schubarti to 2n=50 for most species studied. The size, number, and position of different cytological markers vary among species and populations of Astyanax. We analyzed the karyotypes of individuals from three Astyanax species (Astyanax abramis, Astyanax altiparanae, and Astyanax eigenmanniorum) from populations not previously analyzed. We describe variations in several cytogenetic markers and the karyotypic relationships between them, specifically focusing on the characteristics of the conserved and divergent locations of the ribosomal genes. Our data are useful for establishing relationships between species and for investigating the karyotype evolution within the genus. Universidade Estadual Paulista ‘‘Júlio de Mesquita Filho’’ (UNESP) - Laboratório de Citogenética, Departamento de Biologia, Instituto de Biociências, Rio Claro, São Paulo, Brasil. Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Pesca e Aquicultura (CNPASA), Palmas, Tocantins, Brasil Universidade Estadual Paulista ‘‘Júlio de Mesquita Filho’’ (UNESP) - Laboratório de Citogenética, Departamento de Biologia, Instituto de Biociências, Rio Claro, São Paulo, Brasil.

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:27:20Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:31:57Z : No. of bitstreams: 1 2-s2.0-84875254410.pdf: 1264913 bytes, checksum: b4128411a38f745f8c324aff921e956e (MD5) Made available in DSpace on 2014-05-27T11:27:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-12-01 The family Loricariidae with 813 nominal species is one of the largest fish families of the world. Hypostominae, its more complex subfamily, was recently divided into five tribes. The tribe Hypostomini is composed of a single genus, Hypostomus Lacépède, 1803, which exhibits the largest karyotypic diversity in the family Loricariidae. With the main objective of contributing to a better understanding of the relationship and the patterns of evolution among the karyotypes of Hypostomus species, cytogenetic studies were conducted in six species of the genus from Brazil and Venezuela. The results show a great chromosome variety with diploid numbers ranging from 2n=68 to 2n=76, with a clear predominance of acrocentric chromosomes. The Ag-NORs are located in terminal position in all species analyzed. Three species have single Ag-NORs (Hypostomus albopunctatus (Regan, 1908), H. prope plecostomus (Linnaeus, 1758), and H. prope paulinus (Ihering, 1905)) and three have multiple Ag-NORs (H. ancistroides (Ihering, 1911), H. prope iheringi (Regan, 1908), and H. strigaticeps (Regan, 1908)). In the process of karyotype evolution of the group, the main type of chromosome rearrangements was possibly centric fissions, which may have been facilitated by the putative tetraploid origin of Hypostomus species. The relationship between the karyotype changes and the evolution in the genus is discussed. © Anderson Luis Alves et al. Embrapa Pesca e Aquicultura (CNPASA), Palmas, Tocantins Laboratório de Citogenética Univ Estadual Paulista Julio de Mesquita Filho - UNESP, Rio Claro, São Paulo State Departamento de Morfologia Instituto de Biociências Univ Estadual Paulista Julio de Mesquita Filho, Botucatu, SP Instituto Limnológico Universidad de Oriente Caicara del Orinoco, Estado Bolívar Laboratório de Citogenética Univ Estadual Paulista Julio de Mesquita Filho - UNESP, Rio Claro, São Paulo State Departamento de Morfologia Instituto de Biociências Univ Estadual Paulista Julio de Mesquita Filho, Botucatu, SP

Made available in DSpace on 2013-09-27T14:54:32Z (GMT). No. of bitstreams: 1 WOS000311066300008.pdf: 1175671 bytes, checksum: 2a1ac33195576798d7b74cff123632e1 (MD5) Previous issue date: 2012-01-01 Made available in DSpace on 2013-09-30T18:40:00Z (GMT). No. of bitstreams: 1 WOS000311066300008.pdf: 1175671 bytes, checksum: 2a1ac33195576798d7b74cff123632e1 (MD5) Previous issue date: 2012-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:52:35Z No. of bitstreams: 1 WOS000311066300008.pdf: 1175671 bytes, checksum: 2a1ac33195576798d7b74cff123632e1 (MD5) Made available in DSpace on 2014-05-20T13:52:35Z (GMT). No. of bitstreams: 1 WOS000311066300008.pdf: 1175671 bytes, checksum: 2a1ac33195576798d7b74cff123632e1 (MD5) Previous issue date: 2012-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The family Loricariidae with about 690 species divided into six subfamilies, is one of the world's largest fish families. Cytogenetic studies conducted in the family showed that among 90 species analyzed the diploid number ranges from 2n=38 in Ancistrus sp. to 2n=96 in Hemipsilichthys gobio Luetken, 1874. In the present study, fluorescence in situ hybridization (FISH) was employed to determine the chromosomal localization of the 18S rDNA gene in four suckermouth armoured catfishes: Kronichthys lacerta (Nichols, 1919), Pareiorhaphis splendens (Bizerril, 1995), Liposarcus multiradiatus (Hancock, 1828) and Hypostomus prope plecostomus (Linnaeus, 1758). All species analyzed showed one chromosome pair with 18S rDNA sequences, as observed in the previous Ag-NORs analyses. The presence of size and numerical polymorphism was observed and discussed, with proposing a hypothesis of the Ag-NOR evolution in Loricariidae. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Pesca & Aquicultura CNPASA, Palmas, Tocantins, Brazil Univ Estadual Paulista Julio de Mesquita Filho UN, Lab Citogenet, Rio Claro, São Paulo State, Brazil Univ Estadual Paulista, Dept Morfol, Inst Biociencias, Botucatu, São Paulo State, Brazil Univ Oriente, Inst Limnol, Caicara Del Orinoco, Estado Bolivar, Venezuela Univ Estadual Paulista Julio de Mesquita Filho UN, Lab Citogenet, Rio Claro, São Paulo State, Brazil Univ Estadual Paulista, Dept Morfol, Inst Biociencias, Botucatu, São Paulo State, Brazil

The cachara (Pseudoplatystoma reticulatum) is a Neotropical freshwater catfish from family Pimelodidae (Siluriformes) native to Brazil. The species is of relative economic importance for local aquaculture production and basic biological information is under development to help boost efforts to domesticate and raise the species in commercial systems. The complete cachara mitochondrial genome was obtained by assembling Illumina RNA-seq data from pooled samples. The full mitogenome was found to be 16,576 bp in length, showing the same basic structure, order, and genetic organization observed in other Pimelodidae, with 13 protein-coding genes, 2 rNA genes, 22 trNAs, and a control region. Observed base composition was 24.63% T, 28.47% C, 31.45% A, and 15.44% G. With the exception of NAD6 and eight tRNAs, all of the observed mitochondrial genes were found to be coded on the H strand. A total of 107 SNPs were identified in P. reticulatum mtDNA, 67 of which were located in coding regions. Of these SNPs, 10 result in amino acid changes. Analysis of the obtained sequence with 94 publicly available full Siluriformes mitogenomes resulted in a phylogenetic tree that generally agreed with available phylogenetic proposals for the order. The first report of the complete Pseudoplatystoma reticulatum mitochondrial genome sequence revealed general gene organization, structure, content, and order similar to most vertebrates. Specific sequence and content features were observed and may have functional attributes which are now available for further investigation.

The family Loricariidae, with about 683 species, is one the largest fish families in the world. The subfamily Hypostominae was recently reviewed and is now divided in five tribes. With the main objective of contributing to a better understanding of the relationships of the members of the subfamily Hypostominae, cytogenetic analyses were conducted in seven species (three Hypostomini, three Pterygoplichthini and two Ancistrini) from Brazil and Venezuela. In Pterygoplichthini, all species show 2n = 52 chromosomes. In Hypostomini Hypostomus ancistroides has 2n = 68, H. regani 2n = 72 and Hypostomus goyazensis 2n = 72 chromosomes. In Ancistrini Ancistrus n. sp. 1 has 2n = 39/40 with a sex chromosome system of the type XX/X0, which is a novelty for neotropical fishes, and Ancistrus n. sp. 2 has 2n = 52 chromosomes. Six species have single Ag-NORs and two multiple Ag-NORs. The possible cytogenetic relationships among the species of Hypostominae are discussed.

The family Loricariidae with about 690 species divided into six subfamilies, is one of the world's largest fish families. Recent studies have shown the existence of several problems in the definition of natural groups in the family, which has made the characterization of the subfamilies and even of some genera quite difficult. With the main objective of contributing for a better understanding of the relationships between loricariids, cytogenetic analysis were conducted with two species of Neoplecostominae and nine species of Hypostominae that, according to morphological and molecular data, may belong to a new monophyletic unit. The results obtained showed a marked chromosomal conservation with the presence of 2n = 54 chromosomes and single interstitial Ag-NORs in all species analyzed. Considering that Neoplecostominae is the primitive sister-group of all other loricariids, with exception of Lithogeneinae, this karyotypic structure may represent the primitive condition for the family Loricariidae. The cytogenetic characteristics partaken by the species of Neoplecostominae and Hypostominae analyzed in the present study reinforce the hypothesis that the species of both these subfamilies might belong to a natural group.

Made available in DSpace on 2013-09-27T14:54:33Z (GMT). No. of bitstreams: 1 WOS000303289800004.pdf: 1465158 bytes, checksum: 902d6ca7f1dddc05b480427431a95087 (MD5) Previous issue date: 2012-01-01 Made available in DSpace on 2013-09-30T18:39:58Z (GMT). No. of bitstreams: 1 WOS000303289800004.pdf: 1465158 bytes, checksum: 902d6ca7f1dddc05b480427431a95087 (MD5) Previous issue date: 2012-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:52:34Z No. of bitstreams: 1 WOS000303289800004.pdf: 1465158 bytes, checksum: 902d6ca7f1dddc05b480427431a95087 (MD5) Made available in DSpace on 2014-05-20T13:52:34Z (GMT). No. of bitstreams: 1 WOS000303289800004.pdf: 1465158 bytes, checksum: 902d6ca7f1dddc05b480427431a95087 (MD5) Previous issue date: 2012-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Cytogenetic analyses were accomplished in two populations of Astyanax altiparanae Garutti & Britzki, 2000 and one population of Hyphessobrycon eques Steindachner, 1882, considered incertae sedis in Characidae family. Two populations of A. altiparanae (Mogi-Guacu and Tiete rivers) presented 2n=50, with the same karyotype formula: 6M+12SM+20ST+12A (FN=88). H. eques from Capivara river presented 2n=52 and karyotype formula 14M+16SM+4ST+18A (FN=86). In each karyotype, the nucleolus organizer regions were detected at the end of the short arm of a single medium-sized subtelocentric chromosome. The Chromomycin A(3) (CMA(3)) marking is coincident for the NORs in chromosomes of the two species and present additionally in two different chromosomes of A. altiparanae thus showing interpopulation differences in this species. In H. eques, weak heterochromatic blocks in the position of centromeres and telomeres of most chromosomes and negative C-banding for the NOR bearing chromosome were visualized. The obtained results contribute both to the understanding of karyotype evolution of these species and to the clarifying their phylogenetic relationships. Univ Estadual Paulista, Dept Morfol, Lab Biol Genet Peixes, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil Univ Estadual Paulista, Dept Biol, BR-13506900 Rio Claro, SP, Brazil Univ Estadual Paulista, Dept Morfol, Lab Biol Genet Peixes, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil Univ Estadual Paulista, Dept Biol, BR-13506900 Rio Claro, SP, Brazil CNPq: 140644/2005-9

Cytogenetic analysis in 2 populations of Astyanax scabripinnis from Sao Domingos and Tamboara streams, affluents of the Ivai river basin, was carried out. Both populations presented same diploid of 2n=48 chromosomes, although they might be differentiated by karyotypic formulae (10M, 20SM, 8ST, 10A and 10M, 24SM, 6ST, 8A, respectively) and number of nucleolar organizer chromosomes (3 and 7 NORs, respectively). Besides the population from Sao Domingos showed 1 metacentric B microchromosome restricted to females. Low frequency of this B microchromosome suggests a recent origin in populations of Astyanax scabripinnis and its adaptive function remains unknown. Aspects related to karyotype diversity of this "species complex" are discussed, contributing to the understanding of the mechanisms involved in the diversity and speciation on the group.

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:21:57Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:44:00Z : No. of bitstreams: 1 2-s2.0-33750726429.pdf: 136054 bytes, checksum: 6e3b51e1a8fbeef6379644f130d1ace2 (MD5) Made available in DSpace on 2014-05-27T11:21:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-09-01 The karyotype of a fish species belonging to the family Scoloplacidae, Scoloplax distolothrix, is described for the first time. Cytogenetic studies showed that this species has 2n=50 chromosomes, 18 metacentrics, 18 submetacentrics, 10 subtelocentrics and 4 acrocentrics. The Ag-NORs are restricted to a single large metacentric pair in the interstitial position on the long arm. © 2006 The Japan Mendel Society. Departamento de Morfologia Instituto de Biociências Universidade Estadual Paulista, 18618-000 Botucatu, São Paulo Departamento de Morfologia Instituto de Biociências Universidade Estadual Paulista, 18618-000 Botucatu, São Paulo

In this study, phylogenetic and phylogeographic analyses of populations identified as Hypostomus strigaticeps from the upper Paraná River basin were conducted in order to test whether these different populations comprises cryptic species or structured populations and to assess their genetic variability. The sequences of the mitochondrial DNA ATP sintetase (subunits 6/8) of 27 specimens from 10 populations (one from Mogi-Guaçu River, five from Paranapanema River, three from Tietê River and one from Peixe River) were analyzed. The phylogeographic analysis showed the existence of eight haplotypes (A-H), and despite the ancestral haplotype includes only individuals from the Tietê River basin, the distribution of H. strigaticeps was not restricted to this basin. Haplotypes A, B and F were the most frequent. Haplotypes D, E, F, G, and H were present in the sub-basin of Paranapanema, two (A and B) were present in the sub-basin of the Tietê River, one (C) was exclusively distributed in the sub-basin of the Peixe River, and one (B) was also present in the sub-basin of the Grande River. The phylogenetic analysis showed that the populations of H. strigaticeps indeed form a monophyletic unit comprising two lineages: TG, with representatives from the Tietê, Mogi-Guaçu and Peixe Rivers; and PP, with specimens from the Paranapanema River. The observed degree of genetic divergence within the TG and PP lineages was 0.1% and 0.2%, respectively, whereas the genetic divergence between the two lineages themselves was approximately 1%. The results of the phylogenetic analysis do not support the hypothesis of existence of crypt species and the phylogeographic analysis confirm the presence of H. strigaticeps in other sub-basins of the upper Paraná River: Grande, Peixe, and Paranapanema sub-basins, The authors are grateful to Alessandro G. Bifi, Fábio F. Roxo, and Renato Devidé by helping to collect fishes in the fieldworks; to Cristiane Marcia Mileo and Edson Lourenço da Silva to image editing; to Bioedit® by helping with the English language; to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for financial support (process number: 07/58641-0). Nupélia provide logistic support, Sí