212 results on '"A. Botticelli"'
Search Results
2. Influence on marginal bone levels at implants equipped with blades aiming to control the lateral pressure on the cortical bone. An experimental study in dogs
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Kanayama, Masatsugu, Ferri, Mauro, Guzon, Fernando M. Muñoz, Asano, Akihisa, Alccayhuaman, Karol Alí Apaza, Rossi, Ermenegildo Federico De, and Botticelli, Daniele
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- 2024
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3. Critical-sized marginal defects around implants treated with xenografts in rabbits
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Asano, Akihisa, Xavier, Samuel Porfirio, Silva, Erick Ricardo, Morinaga, Kenzo, Botticelli, Daniele, Nakajima, Yasushi, and Baba, Shunsuke
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- 2024
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4. Application of the break-even point to express the bone dynamics around implants
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Hori, Yoshihiko, Amari, Yoshiyuki, Ohnishi, Hiroyuki, Seo, Hitoshi, Chi, Yeh-Chun, Botticelli, Daniele, Xavier, Samuel Porfirio, and Baba, Shunsuke
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- 2024
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5. Sequential Bone Repair in Rabbit Sinus Lifts Using Bio-Oss and Hyaluronic Acid–Polynucleotide Gel (Regenfast)
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Nozomi Maniwa, Samuel Porfirio Xavier, Sergio Luis Scombatti de Souza, Erick Ricardo Silva, Daniele Botticelli, Kenzo Morinaga, and Shunsuke Baba
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animal study ,bone healing ,histology ,bone augmentation ,Regenfast ,biomaterial ,Biotechnology ,TP248.13-248.65 ,Medicine (General) ,R5-920 - Abstract
Background: A comprehensive investigation to associate the use of polynucleotides and hyaluronic acid with bovine bone in maxillary sinus lift procedures in rabbits has not been performed yet. The aim was to evaluate the influence of this novel association on the processes of bone regeneration in maxillary sinus augmentation. Methods: In this prospective, randomized, within-animal model, maxillary sinus augmentation was performed bilaterally in 12 rabbits. Deproteinized bovine bone material (DBBM) was used as filler material. A hyaluronic acid–polynucleotide gel was aggregated at the test site. Histological evaluations were performed after 2 and 10 weeks of healing. Results: After 2 weeks of healing, similar amounts of new bone were observed at both the control (7.7 ± 4.3%) and test sites (8.1 ± 3.8%; p = 0.697). Bone formation was observed predominantly along the osteotomy margins and adjacent sinus walls. After 10 weeks of healing, the total new bone fraction reached 28.0 ± 10.1% at the control sites and 27.3 ± 10.5% at the test sites (p = 0.563). Multiple perforations of the sinus mucosa were observed in both groups when in contact with the biomaterial granules. Conclusion: The present study failed to demonstrate a positive effect on bone formation when incorporating hyaluronic acid–polynucleotide gel (Regenfast) into a xenograft for maxillary sinus augmentation. Additionally, the use of this gel did not mitigate the occurrence of thinned mucosa or reduce the risk of subsequent sinus mucosa perforation.
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- 2024
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6. Controlled Lateral Pressure on Cortical Bone Using Blade-Equipped Implants: An Experimental Study in Rabbits
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Vitor Ferreira Balan, Mauro Ferri, Eduardo Pires Godoy, Leticia Gabriela Artioli, Daniele Botticelli, Erick Ricardo Silva, and Samuel Porfirio Xavier
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animal study ,bone healing ,histology ,cortical layer ,marginal gap ,Technology ,Biology (General) ,QH301-705.5 - Abstract
Background: This study aimed to evaluate the biological behavior of a novel implant design incorporating decompressive cervical blades. Hence, the aim of the present study was to evaluate the healing outcomes in cortical regions where decompressive protocols were implemented using implants equipped with blades and installed applying a bicortical anchorage. Materials and Methods: Blades with varying diameters were integrated into the coronal portion of the implant to prepare the cortical region of rabbit tibiae. The blade diameters differed from the implant collar by the following amounts: control group (0 µm), +50 µm, and +200 µm. Results: No marginal bone loss was detected. Instead, all implants exhibited new bone formation in the coronal region. Complete closure was observed in the CG-0 group, as well as in the TG-50 and TG-200 groups, despite the presence of marginal gaps without primary bone contact at installation. In the apical region, most implants breached the cortical layer. Nevertheless, new bone formation in this region completely closed the osteotomy, effectively isolating the internal environment of the tibia from the external. Conclusions: The use of a blade incorporated into the implant body enabled precise preparation of the cortical layer, allowing for controlled decompression in the targeted area. This technique resulted in optimal osseointegration with no loss of marginal bone, and complete restoration of marginal gaps ranging from 0 µm to 200 µm.
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- 2024
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7. Sequential Bone Repair in Rabbit Sinus Lifts Using Bio-Oss and Hyaluronic Acid–Polynucleotide Gel (Regenfast).
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Maniwa, Nozomi, Xavier, Samuel Porfirio, Scombatti de Souza, Sergio Luis, Silva, Erick Ricardo, Botticelli, Daniele, Morinaga, Kenzo, and Baba, Shunsuke
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SINUS augmentation ,BONE grafting ,MINERAL aggregate testing ,BONE regeneration ,BONE growth - Abstract
Background: A comprehensive investigation to associate the use of polynucleotides and hyaluronic acid with bovine bone in maxillary sinus lift procedures in rabbits has not been performed yet. The aim was to evaluate the influence of this novel association on the processes of bone regeneration in maxillary sinus augmentation. Methods: In this prospective, randomized, within-animal model, maxillary sinus augmentation was performed bilaterally in 12 rabbits. Deproteinized bovine bone material (DBBM) was used as filler material. A hyaluronic acid–polynucleotide gel was aggregated at the test site. Histological evaluations were performed after 2 and 10 weeks of healing. Results: After 2 weeks of healing, similar amounts of new bone were observed at both the control (7.7 ± 4.3%) and test sites (8.1 ± 3.8%; p = 0.697). Bone formation was observed predominantly along the osteotomy margins and adjacent sinus walls. After 10 weeks of healing, the total new bone fraction reached 28.0 ± 10.1% at the control sites and 27.3 ± 10.5% at the test sites (p = 0.563). Multiple perforations of the sinus mucosa were observed in both groups when in contact with the biomaterial granules. Conclusion: The present study failed to demonstrate a positive effect on bone formation when incorporating hyaluronic acid–polynucleotide gel (Regenfast) into a xenograft for maxillary sinus augmentation. Additionally, the use of this gel did not mitigate the occurrence of thinned mucosa or reduce the risk of subsequent sinus mucosa perforation. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Sinus mucosa thinning and perforation after sinus augmentation. A histological study in rabbits
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Kato, Shingo, Botticelli, Daniele, De Santis, Enzo, Kanayama, Masatsugu, Ferreira, Sabrina, and Rangel-Garcia Jr, Idelmo
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- 2021
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9. Controlled Lateral Pressure on Cortical Bone Using Blade-Equipped Implants: An Experimental Study in Rabbits.
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Ferreira Balan, Vitor, Ferri, Mauro, Pires Godoy, Eduardo, Artioli, Leticia Gabriela, Botticelli, Daniele, Silva, Erick Ricardo, and Xavier, Samuel Porfirio
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COMPACT bone ,RABBITS ,BONE growth ,TIBIA ,OSSEOINTEGRATION - Abstract
Background: This study aimed to evaluate the biological behavior of a novel implant design incorporating decompressive cervical blades. Hence, the aim of the present study was to evaluate the healing outcomes in cortical regions where decompressive protocols were implemented using implants equipped with blades and installed applying a bicortical anchorage. Materials and Methods: Blades with varying diameters were integrated into the coronal portion of the implant to prepare the cortical region of rabbit tibiae. The blade diameters differed from the implant collar by the following amounts: control group (0 µm), +50 µm, and +200 µm. Results: No marginal bone loss was detected. Instead, all implants exhibited new bone formation in the coronal region. Complete closure was observed in the CG-0 group, as well as in the TG-50 and TG-200 groups, despite the presence of marginal gaps without primary bone contact at installation. In the apical region, most implants breached the cortical layer. Nevertheless, new bone formation in this region completely closed the osteotomy, effectively isolating the internal environment of the tibia from the external. Conclusions: The use of a blade incorporated into the implant body enabled precise preparation of the cortical layer, allowing for controlled decompression in the targeted area. This technique resulted in optimal osseointegration with no loss of marginal bone, and complete restoration of marginal gaps ranging from 0 µm to 200 µm. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
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10. New bone ingrowth into β-TCP/HA graft activated with argon plasma: a histomorphometric study on sinus lifting in rabbits
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Kazushige Tanaka, Daniele Botticelli, Luigi Canullo, Shunsuke Baba, and Samuel P. Xavier
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Animal study ,Sinus floor elevation ,Bone healing ,Xenograft ,Argon plasma ,Medicine ,Dentistry ,RK1-715 - Abstract
Abstract Background In a previous experimental study, new bone was found growing within granules of HA/β-TCP. In vitro and experimental studies have shown increased protein adsorption and cell adhesion graft material bioactivated with Argon plasma. The aims of the present experiment were to study new bone ingrowth into β-TCP/HA granules used as filler material for sinus lifting and the influence on the healing of the bioactivation of the graft with argon plasma. Methods Sinus lifting was carried out in 20 rabbits using 60% HA and 40% β-TCP as filler material either bio-activated with argon plasma (plasma group) or left untreated (control group). The antrostomies were closed with collagen membranes. Biopsies representing the healing after 2 and 10 weeks were collected, and ground sections were prepared for histomorphometric analyses. Various regions of the elevated space were analyzed both around (outer bone; OB) and inside (interpenetrating bone network, IBN) the graft particles. Results After 2 weeks of healing, 8.2% and 9.3% (n = 10; p = 0.635) of total new bone (OB + IBN) was found in the plasma and control groups, respectively. Small fractions of IBN were found, spreading from the periphery inward of the graft particles. After 10 weeks of healing, the total new bone was 34.0% in the plasma and 31.3% in Control groups (n = 9; p = 0.594). The respective fractions of IBN were 18.0% and 16.0%. New bone was penetrating from the peripheral regions inside the remnants of graft particles, where it was forming a network of bridges in continuity to the remnants of biomaterial through its porosities. The biomaterial decreased in proportion between 2 and 10 weeks from 52.1 to 28.3% in the plasma group, and from 52.5% to 31.9% in the control group. Conclusion The bio-activation with argon plasma on a synthetic graft composed of 60% HA and 40% β-TCP used as filler material for sinus lifting showed a tendency to improve bone formation; however, the difference with the control group was neither statistically significant nor clinically relevant.
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- 2020
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11. Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep
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Alessandro Perini, Giada Ferrante, Stefano Sivolella, Joaquín Urbizo Velez, Franco Bengazi, and Daniele Botticelli
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Animal study ,Sinus floor elevation ,Bone window ,Bone ,Bone plate ,Polylactic membrane ,Medicine ,Dentistry ,RK1-715 - Abstract
Abstract The objective of this study was to compare the healing of the augmented sinus at which the antrostomy was covered with a membrane or the repositioned bone plate. Eight sheep underwent bilateral maxillary sinus floor augmentation. The control site was covered with a resorbable membrane, while at the experimental site the bone plate was repositioned, and both were secured with cyanoacrylate. Animals were euthanised after 4 months and histomorphometric analysis was performed. A large amount of the graft appeared to be partially interpenetrated by the newly formed bone. Statistical analysis demonstrated different percentages of the new bone and bone interpenetrated to the graft between test and control site in the close-to-window area respectively 22.1 ± 12.6 vs 7.5 ± 4.5 (P = 0.028) and 66.1 ± 14.7 vs 44.2 ± 15.1 (P = 0.046). Other areas showed no difference in the bone and graft amount. More bone was found at the edges of the antrostomy in the experimental site, without statistical significance. In the centre of the antrostomy, the replaced bony window appeared bonded to the newly formed bone. No remnants and no biological response to cyanoacrylate were observed. The repositioning of the bony window after sinus floor elevation in sheep led to a larger amount of newly formed bone in the close-to-window zone of the grafted area. The bony window appeared partially bonded to the new bone. Newly formed bone was found interpenetrating the graft granules.
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- 2020
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12. Bone healing at collagenated bicortically installed implants: an experimental study in rabbits
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Feletto, Luigi, Bengazi, Franco, Urbizo Velez, Joaquín Juan, Ferri, Mauro, Favero, Riccardo, and Botticelli, Daniele
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- 2020
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13. Comparison of histomorphometry and microCT after sinus augmentation using xenografts of different particle sizes in rabbits
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Iida, Takahisa, Baba, Shunsuke, Botticelli, Daniele, Masuda, Katsuhiko, and Xavier, Samuel P.
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- 2020
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14. Schneiderian Membrane Collateral Damage Caused by Collagenated and Non-Collagenated Xenografts: A Histological Study in Rabbits
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Yasushi Nakajima, Daniele Botticelli, Ermenegildo Federico De Rossi, Vitor Ferreira Balan, Eduardo Pires Godoy, Erick Ricardo Silva, and Samuel Porfirio Xavier
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animal study ,sinus floor elevation ,bone healing ,Schneiderian membrane ,histology ,Dentistry ,RK1-715 - Abstract
Background: The Schneiderian membrane (SM) that is in contact with biomaterial granules may become thinner and eventually perforate. It has been shown that these events are related to the biomaterial used. Hence, the main aim of the present study was to compare the damaging effects of two xenografts with different resorbability rates on SM. The secondary aim was to evaluate the possible protection from damage offered by a collagen membrane placed adjacent to the SM and by inward displacement of the bone window with the SM during elevation. Methods: Thirty-six albino New Zealand rabbits underwent bilateral sinus elevation. One group of 18 animals received deproteinized bovine bone mineral (DBBM group) and the other received swine-collagenated corticocancellous bone (collagenated group). Moreover, in the DBBM group, the bone window was displaced inward during elevation in one sinus together with the SM. In the collagenated group, a collagen membrane was placed adjacent to the SM in one sinus. Six animals were assessed per period after 2, 4, and 8 weeks. Results: The mean pristine mucosa width ranged between 67 µm and 113 µm, and none had a width of
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- 2023
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15. Sinus Mucosal Damage Triggered by Synthetic or Xenogeneic Bone Substitutes: A Histological Analysis in Rabbits
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Yuki Omori, Daniele Botticelli, Stefano Migani, Vitor Ferreira Balan, Eduardo Pires Godoy, and Samuel Porfirio Xavier
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animal study ,sinus floor elevation ,bone healing ,Schneiderian membrane ,histology ,sinus mucosa perforation ,Biotechnology ,TP248.13-248.65 ,Medicine (General) ,R5-920 - Abstract
Background: It has been shown in rabbit models that the sinus mucosa in contact with graft particles might experience a progressive thinning and perforations. The phenomenon depends on the graft used. Hence, the aim of the present study was to compare the damaging effects of a synthetic of a xenogeneic graft. Methods: Forty New Zealand rabbits received a bilateral sinus elevation. Both sinuses of twenty rabbits were grafted with a biphasic 60% hydroxyapatite and 40% β-tricalcium phosphate while the other twenty received a deproteinized bovine bone mineral graft. Thinned sites (p = 0.222 between periods), and to 114 sites in the Xenogeneic group (p = 0.030 between groups; p = 0.001 between periods). Perforations were few in the 2-week period, two in two sinuses out of 20 in the Synthetic group, and four in two sinuses out of 20 in the Xenogeneic group (p = 0.721). In the 10-week period, the perforations increased to eight in the Synthetic group, distributed in six sinuses out of 20, and to sixteen in the Xenogeneic group, distributed in 11 sinuses out of 20 (p = 0.082). The pseudostratified epithelium presented a reduced width at the thinned sites. Conclusions: The contact with synthetic or xenogeneic grafts will induce thinning and possible perforations of the sinus mucosa. This effect will increase over time, and it is stronger at the xenogeneic than the synthetic graft.
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- 2022
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16. Subcrestal Positioning of Implants with a Convergent Hyperbolic Collar Profile: An Experimental Study in Dogs.
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Mainetti, Tomaso, Bengazi, Franco, Velez, Joaquin Urbizo, derico De Rossi, Ermenegildo Fe, Sakaguchi, Ryuichi, and Botticelli, Daniele
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DENTAL implants ,EXPERIMENTAL design ,ANIMAL experimentation ,RESEARCH funding ,DOGS - Abstract
Purpose: To evaluate the influence on peri-implant soft and hard tissues of one-piece implants with a convergent hyperbolic profile collar placed at different depths with respect to the bone crest. Materials and Methods: Six dogs were included in the experiment. Three months after mandibular tooth extractions, two one-piece implants carrying a 2.8-mm--high convergent hyperbolic profile collar were placed in the alveolar crest with the coronal margin of the rough surface either 0.8 mm (test-1) or 1.8 mm (test-2) deeper with respect to the bone crest (Ct0). Two similar implants were instead placed flush to Ct0 as controls (control-1 and control-2, respectively). Healing screws were connected, and nonsubmerged healing was allowed. After 4 months, block sections were harvested, and histologic slides were prepared in a buccolingual plane. Results: In the histologic analyses, both the buccal crest and coronal level of osseointegration were located more coronally at the test compared to the control implants concerning the implant. However, the buccal bone crest with respect to Ct0 presented a loss of 0.8 ± 0.4 mm at the test-1 and 0.5 ± 0.4 mm at the control-1 implants (P = .028), and a loss of 2.0 ± 1.0 mm and 0.7 ± 0.4 mm at the test-2 and control-2 implants (P = .028), respectively. At the control implants, the collars were exposed above the peri-implant mucosa, while those of the test implants were not. However, the coronal level of the peri-implant mucosa with respect to Ct0 was located more apically at the test compared to the control implants. Conclusion: The placement of implants with a hyperbolic convergent profile collar in the subcrestal position resulted in higher buccal bone resorption and more soft tissue recession compared to the crestal implants with respect to the level of the bone crest at placement. [ABSTRACT FROM AUTHOR]
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- 2022
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17. Clinical and Histological Healing after Maxillary Sinus Floor Elevation
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Daniele Botticelli
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alloplastic ,animal study ,antrostomy ,biomaterial ,bone substitute ,collagen membrane ,Dentistry ,RK1-715 - Abstract
Sinus floor elevation is a surgical procedure that allows for the insertion of the implant in the posterior region of the maxilla in case of insufficient volume of native bone. Several studies have reported a high success rate, and this has helped to spread this surgical procedure around the world. The subject has been extensively studied and this could lead researchers to think that no new scientific evidence can be provided. However, new ideas and discoveries show that research never reaches a conclusion, and that new information can be added all the time. This collection aimed to provide new evidence that could be added to daily clinical work and to provide new ideas for future research.
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- 2022
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18. New bone ingrowth into β-TCP/HA graft activated with argon plasma: a histomorphometric study on sinus lifting in rabbits
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Tanaka, Kazushige, Botticelli, Daniele, Canullo, Luigi, Baba, Shunsuke, and Xavier, Samuel P.
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- 2020
- Full Text
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19. Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep
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Perini, Alessandro, Ferrante, Giada, Sivolella, Stefano, Velez, Joaquín Urbizo, Bengazi, Franco, and Botticelli, Daniele
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- 2020
- Full Text
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20. Sinus Mucosa Thinning and Perforations after Sinus Lifting Performed with Different Xenografts: A Histological Analysis in Rabbits
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Riccardo Favero, Karol Alí Apaza Alccayhuaman, Daniele Botticelli, Samuel Porfirio Xavier, Vitor Ferreira Balan, Veronica Macchi, and Raffaele De Caro
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animal study ,sinus floor elevation ,bone healing ,Schneiderian membrane ,histology ,Dentistry ,RK1-715 - Abstract
Background: Experimental studies have shown a progressive thinning and perforations of the sinus mucosa associated with sharpened edges and the cutting projections of graft particles used simultaneously for maxillary sinus augmentation. Hence, the aim of the present study was to evaluate the damaging effects of two different bovine grafts on the sinus mucosa after sinus augmentation. Methods: Twenty New Zealand rabbits received a bilateral sinus lifting using, as fillers, two different types of deproteinized bovine bone in granules, one processed at low temperature (low-T group), and the other at high temperature (high-T group). Thinned mucosa sites (p = 0.191). At the 10-week assessment, the thinned sites increased to 237 and 195 sites, respectively. The numbers of sinus mucosa perforations after 2 weeks were eight and three in the low-T and high-T group, respectively. At the 10-week evaluation, the perforations increased to 19 in the low-T group, and to 14 in the high-T group. Conclusions: The contact with bovine xenografts yielded thinning and perforations of the sinus mucosa. Despite the differences in characteristics and dimensions, no differences were found between the two xenografts in the numbers of thinning mucosa sites and perforations. However, a trend of more events was found in the low-T compared to the high-T group.
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- 2021
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21. Influence of the Use of a Collagen Membrane Placed on the Bone Window after Sinus Floor Augmentation—An Experimental Study in Rabbits
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Alessandro Perini, Jose Viña-Almunia, Carmen Carda, José Javier Martín de Llano, Daniele Botticelli, and Miguel Peñarrocha-Diago
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animal study ,sinus floor elevation ,bone healing ,osteoconductivity ,histology ,morphometry ,Dentistry ,RK1-715 - Abstract
Background: We studied the influence on healing of a resorbable membrane covering the osteotomy site after maxillary sinus grafting, evaluated in different regions of the augmented area. Methods: Maxillary sinus augmentation was performed in 24 New Zealand rabbits. Osteotomy, 4 × 6 mm, were performed bilaterally. A collagenated cortico-cancellous porcine bone was used to fill the elevated region. A collagen membrane was randomly placed over the osteotomy site on one side (MG), and the other side was left uncovered (NMG). The animals were euthanized after 2, 4, and 8 weeks; and histomorphometric analysis was performed in eight different regions. Results: New bone percentages were similar in both groups. There were no statistically significant differences. In MG, the overall percentages were 15.6 ± 7.3%, 22.9 ± 6.1%, and 24.9 ± 12.0% after 2, 4, and 8 weeks, respectively. In NMG, the percentages were 11.2 ± 4.5%, 24.1 ± 5.7%, and 24.5 ± 15.7%, respectively. The proportions of new bone in the various regions after 8 weeks were 31 ± 8.9% and 29.9 ± 9.1% in the bone walls region, 25 ± 10.1% and 32.8 ± 9.1% in the submucosa region, 22.6 ± 21.6% and 10.9 ± 11.5 in the middle region, 17.3 ± 14% and 13.4 ± 9.8% in the close-to-window region, and 21.8 ± 11.6%, 19.1 ± 6.4% in the osteotomy region—for MG and NMG, respectively. Conclusions: In both groups the greatest amounts of bone formation occurred near to the pre-existing bone walls, followed by the sub-mucosa region. The smallest amounts were found in the close-to-window region, followed by the central region. The placement of a collagen membrane to cover the osteotomy site did not influence the amount of new bone formation after sinus grafting.
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- 2021
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22. Argon Bioactivation of Implants Installed Simultaneously to Maxillary Sinus Lifting without Graft. An Experimental Study in Rabbits
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Yuki Omori, Daniele Botticelli, Mauro Ferri, Rafael Delgado-Ruiz, Vitor Ferreira Balan, and Samuel Porfirio Xavier
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animal study ,sinus floor elevation ,bone healing ,Schneiderian membrane ,histology ,Dentistry ,RK1-715 - Abstract
Background: The treatment of the surface of titanium implants with argon plasma improved its hydrophilicity and cell adhesion, resulting in higher bone apposition on implant and graft surfaces. The spontaneous perforation over time of the sinus mucosa after sinus augmentation has been documented in experimental studies at both implants and graft particles. The aim of the present study was to evaluate the influence of plasma argon treatment of the implant surface on bone apposition and on the rate of sinus mucosa perforations. Methods: A sinus lifting procedure was performed bilaterally in sixteen rabbits, and implants, either treated with argon plasma or left without treatment (control), were placed simultaneously without grafts. After 8 weeks, histological analyses were carried out. Results: A collapse of the sinus mucosa was observed at all implants. Twenty-four out of thirty-two implants presented sinus mucosa perforations at the apex. Several perforations were also found at the threads. Thinned mucosa sites (width < 40 µm) were found around almost all implants. About 2.6–2.9 mm of the apical regions of the implant did not present signs of osseointegration and about 1.3 mm were exposed to the sinus cavity. No statistically significant differences were found between plasma and control sites. Conclusions: In conclusion, the sinus mucosa was damaged and perforated by direct contact with treated and non-treated implant surfaces. The treatment of the implant surface with argon plasma did not affect the outcomes.
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- 2021
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23. Maxillary Sinus Augmentation Using Ceramic Alloplastic Granules or Paste: An Experimental Study in Rabbits
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Michael Medeiros Costa, Daniele Botticelli, Ofer Moses, Yuki Omori, Shigeo Fujiwara, Erick Ricardo Silva, and Samuel Porfirio Xavier
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animal study ,sinus floor elevation ,bone healing ,histomorphometry ,alloplastic ,collagen membrane ,Dentistry ,RK1-715 - Abstract
Background: Due to the lack of data comparing the biological behavior of two formulations, granules and paste, of alloplastic graft from microtomographic and histomorphometric points of view, the aim of the present experiment was to compare the histomorphometric and microtomographic healing of two formulations, i.e., granules (MR sites) or paste (MR-inject sites) of an alloplastic graft composed of a combination of beta-tricalcium phosphate and hydroxyapatite used for maxillary sinus lifting. Methods: A sinus lifting procedure was carried out bilaterally in 20 rabbits, and the elevated space was filled with either paste or granules of an alloplastic material. A collagen membrane was placed on the antrostomy and the animals were euthanized after 2 or 10 weeks, 10 animals each group. Microtomographic and histological analyses were performed. Results: Higher proportions of new bone formation were found at the MR, compared to the MR-inject sites both after 2 weeks (2.65 ± 2.89% vs. 0.08 ± 0.12%; p < 0.01) and 10 weeks of healing (34.20 ± 13.86 vs. 23.28 ± 10.35%; p = 0.022). Conclusions: It was concluded that new bone formation was faster in the MR sites, compared to the MR-inject. However, a longer time of healing should be allowed to make final conclusions about the efficiency in bone formation of the paste formulation of the biomaterial used in the present study.
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- 2021
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24. Osteoconductivity of Bovine Xenograft Granules of Different Sizes in Sinus Lift: A Histomorphometric Study in Rabbits
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Eduardo Pires Godoy, Karol Alí Apaza Alccayhuaman, Daniele Botticelli, Andrea Amaroli, Vitor Ferreira Balan, Erick Ricardo Silva, and Samuel Porfirio Xavier
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animal study ,sinus floor elevation ,bone healing ,osteoconductivity ,histology ,morphometry ,Dentistry ,RK1-715 - Abstract
Background: Due to the lack of data on bone-to-graft contact (BGC) over time in the various regions within the subantral space of the augmented sinus floor, the present study aimed to evaluate the osteoconductivity of deproteinized bovine bone mineral (DBBM) with granules of different sizes applied in maxillary sinus floor elevation. Methods: A maxillary sinus augmentation was performed bilaterally in 18 rabbits using DBBM with particle dimensions of either 0.125–1.0 mm or 1–2 mm. The antrostomy was covered using a collagen barrier. The animals were euthanized in groups of six after 2, 4, and 8 weeks of healing. MicroCT and histological analyses were performed. Results: After 2 weeks of healing, BGC was 10.9% and 11.9% for the small and large granule sites, respectively. After 8 weeks of healing, the BGC increased to 65% and 62% at the small and large granule sites, respectively. The highest values were located close to the bony walls and the bony window. New bone content developed between 2 and 8 weeks from 7.0% to 27.6% and from 6.1% to 27.6% at the small and large granule sites, respectively. Conclusions: Similar outcomes in osteoconductivity and bone formation were found at both small and large DBBM granule sites.
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- 2021
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25. Implants Placed with a Ring Technique Using Inlay and Onlay Block Xenografts in the Mandible of Rabbits.
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Kaneko, Naoki, Xavier, Samuel Porfirio, Morinaga, Kenzo, Botticelli, Daniele, Silva, Erick Ricardo, Nakajima, Yasushi, and Baba, Shunsuke
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OSSEOINTEGRATION ,MANDIBLE ,XENOGRAFTS ,BONE grafting ,BONE growth ,CANCELLOUS bone ,RABBITS - Abstract
Background: Xenogenous bone has been proposed as an alternative to overcome the disadvantages of autogenous grafting. The aim of the present study was to study bone dynamics at inlay and onlay xenografts used for bone augmentation applying a ring technique. Methods: The bone at the lateral surface of the mandibular angle of 12 adult male New Zealand White rabbits was exposed bilaterally. The cortical layer received multiple perforations on one side of the mandible, and a xenograft block of collagenated cancellous equine bone, 7 mm in diameter and 3 mm in width, was fixed on the prepared surface using an implant (onlay group). On the opposite side, a defect 7 mm in diameter and 3 mm in depth was prepared, and the xenograft block was adapted to the defect and fixed with an implant (inlay group). Results: After ten weeks of healing, in the onlay grafts, new bone was mainly formed on the trabeculae surface, reaching in some specimens the most coronal regions of the block. In the inlay grafts, new bone was found arranged on the trabecular surfaces but also occupying the spaces among the trabeculae. The entrance of the defect was often found close to the top of the block by newly formed bone. A higher percentage of new bone was found in the inlay (19.0 ± 9.3%) compared to the onlay (10.4 ± 7.4%) groups (p = 0.031). The mean gain in osseointegration at the implant in relation to the base of the original 3 mm deep defect was 0.95 ± 1.05% in the onlay group and 0.78 ± 0.71% in the inlay group (p = 0.603). Conclusion: The inlay grafts exhibited a higher new bone percentage than the onlay block grafts possibly due to the defect conformation that presented more sources for bone growth. The trabecular conformation and the composition of the grafts made possible the expression of the osteoconductive properties of the material used. This resulted, in several specimens, in the growth of bone on the graft trabeculae toward the most superior regions in both groups and in the closure of the coronal entrance of the defects in the inlay group. The clinical relevance of this experiment is that the ring technique applied as an inlay method could be suitable for bone augmentation. [ABSTRACT FROM AUTHOR]
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- 2023
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26. Incidence of Sinus Mucosa Perforations During Healing After Sinus Elevation Using Deproteinized Bovine Bone Mineral as Grafting Material: A Histologic Evaluation in a Rabbit Model.
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Miki, Michihide, Botticelli, Daniele, Ricardo Silva, Erick, Xavier, Samuel P., and Baba, Shunsuke
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MAXILLARY sinus surgery ,DENTAL implants ,BIOPSY ,XENOGRAFTS ,ANIMAL experimentation ,BONE substitutes ,RABBITS ,BONE regeneration ,ORAL mucosa - Abstract
Purpose: To examine the reaction of mucosa over time to a close contact with biomaterial after sinus elevation performed with deproteinized bovine bone mineral (DBBM) granules of two different sizes. Materials and Methods: Bilateral sinus mucosa elevation was performed in 18 New Zealand rabbits through access on the nasal dorsum. DBBM with granule dimensions of either 1 to 2 mm (large group) or 0.250 to 1.0 mm (small group) were used to randomly fill the subantral hollow spaces. Biopsy specimens of the experimental sites from six animals in each group were obtained 2, 4, and 8 weeks after the surgery. Results: The integrity of the sinus mucosa was clinically evaluated during surgery using a ×2.5 magnifying visual device. The sinus mucosa in contact with the biomaterial granules was found to be thinned compared with the pristine mucosa in all periods examined. Three hundred fifty-two thinned zones were found considering all 36 sinuses treated. Perforations of the sinus mucosa with extrusion of granules toward the sinus were observed, increasing in number over time. In the 8-week healing period, five perforations in three sinuses and eight perforations in four sinuses were found in the large and small groups, respectively. No differences were seen between the large and small groups. The differences between 2 and 8 weeks were statistically significant for the thinned mucosa width in both the large and the small groups and for the number of sinuses and perforations for the small group. Conclusion: Thinning zones and perforations of the sinus mucosa were seen increasing in number over time in regions in contact with graft granules in both the large and small groups. [ABSTRACT FROM AUTHOR]
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- 2021
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27. The Influence on Healing of Bony Window Elevated Inward in the Sinus Cavity as Cortical Bone Graft: A Histomorphometric Study in Rabbit Model.
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Amari, Yoshiyuki, Botticelli, Daniele, Apaza Alccayhuaman, Karol Alí, Hirota, Atsuya, Silva, Erick Ricardo, and Xavier, Samuel Porfirio
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MAXILLARY sinus surgery ,ANIMAL experimentation ,BIOLOGICAL models ,BONE grafting ,COMPACT bone ,COMPUTED tomography ,GUIDED tissue regeneration ,RABBITS ,WOUND healing ,XENOGRAFTS ,BONE density ,MANN Whitney U Test - Abstract
Purpose: To evaluate the influence on healing of the bony window elevated inward in the sinus cavity as a cortical bone graft. Materials and Methods: Eighteen rabbits were included in the experiment. At the test sites (bony window), the antrostomy was prepared and the remaining bony window was elevated together with the sinus mucosa. At the control sites, the bony window was gently detached before the sinus mucosa elevation and discarded. The space obtained was grafted with deproteinized bovine bone mineral (DBBM). A collagen membrane was positioned on the antrostomy at both sides. The rabbits were euthanized after 2, 4, and 8 weeks in groups of six each. Histologic analyses in different regions of the elevated space were carried out, and a Wilcoxon test was used to estimate differences. Microcomputed tomography (microCT) analyses were also performed. Results: After 2 weeks of healing, higher proportions of new bone were found in the test group compared with the control group due to the higher amount of bone formed in the region subjacent to the sinus mucosa. In this region, higher amounts of new bone were also found in the test group after 4 (P = .028) and 8 weeks of healing (P = .345). After 8 weeks of healing, the percentage of new bone was higher at the control sites compared with the test sites, with the proportions being 25.4% ± 3.2% and 21.3% ± 6.1%, respectively. In this period of evaluation, the bony window contributed with 20.1% ± 5.3% of vital bone in the test group. A fraction of 60.4% ± 10.8% of its surface was surrounded by new bone. In the microCT analysis, after 8 weeks of healing, fractions of 22.3% ± 1.6% and 22.2% ± 0.7% of bone were found in the test and control groups, respectively. Conclusion: The presence of the bony window positively influenced the healing in the elevated space, especially in the submucosa region. The bony window was vital and incorporated into newly formed bone. [ABSTRACT FROM AUTHOR]
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- 2020
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28. Histological Comparison of Collagenated Cancellous Equine Bone Blocks Used as Inlay or Onlay for Lateral Bone Augmentation in Rabbits.
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Sakaguchi, Ryuichi, Xavier, Samuel Porfirio, Morinaga, Kenzo, Botticelli, Daniele, Silva, Erick Ricardo, Nakajima, Yasushi, and Baba, Shunsuke
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BONE grafting ,BONE growth ,COMPACT bone ,RABBITS ,BONE remodeling ,BONE regeneration - Abstract
Background: The conformation of the recipient site for an inlay graft presents an increased contact with the parent bone compared to an onlay graft. This might favor bone growth within the inlay compared to onlay grafts. Hence, the objective of this study was to compare the bone incorporation and remodeling processes of xenogeneic en bloc grafts placed using two bone grafting techniques, i.e., onlay vs. inlay. Methods: In this prospective, randomized, split-mouth study (test and control sides in the same animal), two bone grafting techniques were comparatively evaluated. The lateral aspect of the rabbit mandible was used as the recipient site, bilaterally. On one side of the mandible, the cortical bone was perforated with drills to allow a better bone formation from the bone wound and the marrow spaces. A xenogeneic bone block was fixed in the center of the prepared region, representing the onlay site. On the other side of the mandible, a 7 mm wide and 3 mm deep circumferential defect was prepared using trephines and drills. A xenogeneic bone block was fixed in the center of the defect, representing the inlay site. Two healing periods were applied in the study: 2 and 10 weeks, each represented by 10 rabbits (n = 10 for each period). Results: After 2 weeks of healing, the mean percentage of new bone was 10.4% and 23.3% at the onlay and inlay grafts, respectively (p = 0.022). After 10 weeks of healing, new bone increased to 13.2% at the onlay sites and 25.4% at the inlay sites (p = 0.080). In the 10-week period, the inlay grafts presented a homogeneous growth of new bone in all regions, while in the onlay grafts, low percentages of new bone were observed in the external regions. Conclusion: The percentage of new bone increased faster and was higher in the inlay grafts than in the onlay grafts. This outcome might be related to the self-contained conformation of the recipient site in the inlay group, which offered more sources for new bone formation compared to the one-wall conformation of the recipient sites in the onlay group. The osteoconductive properties of the biomaterial allowed the newly formed bone to reach the most peripheral regions in both groups. The osteoconductive properties of the biomaterial, together with the protection offered by the collagen membrane, allowed marginal closure of the defects by newly formed bone in the inlay group. [ABSTRACT FROM AUTHOR]
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- 2023
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29. Sinus mucosa thinning and perforation after sinus augmentation. A histological study in rabbits
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Enzo De Santis, Daniele Botticelli, Shingo Kato, Sabrina de Souza Ferreira, Idelmo Rangel-Garcia, Masatsugu Kanayama, ARDEC Academy, Fundación ARDEC, and Universidade Estadual Paulista (Unesp)
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Animal study ,Perforation (oil well) ,Sinus Floor Augmentation ,Bone healing ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Tibia ,Autogenous bone ,030223 otorhinolaryngology ,Close contact ,Sinus (anatomy) ,Wound Healing ,Perforation ,Bone Transplantation ,business.industry ,Xenograft ,Dental Implantation, Endosseous ,030206 dentistry ,Anatomy ,Maxillary Sinus ,Bovine bone ,medicine.anatomical_structure ,Otorhinolaryngology ,Sinus floor elevation ,Face ,Bone Substitutes ,Cattle ,Surgery ,Rabbits ,Implant ,Oral Surgery ,business - Abstract
Made available in DSpace on 2021-06-25T10:22:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Aim: To evaluate the modifications and possible perforations of the sinus mucosa lining graft particles and implant surfaces after sinus lifting. Material and methods: Twelve New Zealand rabbits underwent a bilateral sinus lifting using either autogenous bone harvested from the tibia (AB; autogenous bone group) or deproteinized bovine bone mineral (DBBM group) as filler. Implants were simultaneously installed. Thinned sites (
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- 2021
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30. Antrostomy Preparation for Maxillary Sinus Floor Augmentation Using Drills or a Sonic Instrument: A Microcomputed Tomography and Histomorphometric Study in Rabbits.
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Masuda, Katsuhiko, Silva, Erick Ricardo, Botticelli, Daniele, Apaza Alccayhuaman, Karol Alí, and Xavier, Samuel P.
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SINUS augmentation ,HISTOMORPHOMETRY ,MAXILLARY sinus surgery ,COMPUTED tomography ,MAXILLARY sinus ,WOUND healing - Abstract
Purpose: To evaluate healing after sinus floor augmentation at antrostomies prepared with either a drill or a sonic instrument. Materials and Methods: Sinus mucosa elevation was performed bilaterally in 18 rabbits. The antrostomy was prepared with either a drill or a sonic instrument. The elevated space was filled with a collagenated porcine bone, and the access antrostomy was covered by a collagen membrane. The animals were euthanized in groups of six after 2, 4, and 8 weeks of healing. Microcomputed tomography and a histologic analysis were performed. Results: After 8 weeks of healing, remaining defects occupying about 28.9% and 26.0% of the antrostomy were found in the microcomputed tomography analysis. In the histologic analysis, new bone was formed from the margins of the antrostomy, reaching the central region over time. After 8 weeks of healing, 29.1% ± 18.6% and 28.4% ± 15.6% of new mineralized bone was occupying the analyzed zone in the antrostomy at the drill and sonic sites, respectively. Conclusion: The use of a sonic device to prepare the antrostomy resulted in similar healing outcomes in both the antrostomy and the elevated regions. [ABSTRACT FROM AUTHOR]
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- 2019
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31. Influence of the Use of a Collagen Membrane Placed on the Bone Window after Sinus Floor Augmentation—An Experimental Study in Rabbits
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José Javier Martín de Llano, Carmen Carda, Daniele Botticelli, Jose Viña-Almunia, Alessandro Perini, and Miguel Peñarrocha-Diago
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Sinus Floor Augmentation ,Maxillary sinus ,medicine.medical_treatment ,antrostomy ,Bone healing ,Osteotomy ,osteoconductivity ,Article ,histology ,Submucosa ,Porcine bone ,medicine ,xenograft ,General Dentistry ,business.industry ,Collagen membrane ,collagen membrane ,Histology ,RK1-715 ,animal study ,medicine.anatomical_structure ,Dentistry ,sinus floor elevation ,bone healing ,business ,Nuclear medicine ,morphometry - Abstract
Background: We studied the influence on healing of a resorbable membrane covering the osteotomy site after maxillary sinus grafting, evaluated in different regions of the augmented area. Methods: Maxillary sinus augmentation was performed in 24 New Zealand rabbits. Osteotomy, 4 × 6 mm, were performed bilaterally. A collagenated cortico-cancellous porcine bone was used to fill the elevated region. A collagen membrane was randomly placed over the osteotomy site on one side (MG), and the other side was left uncovered (NMG). The animals were euthanized after 2, 4, and 8 weeks, and histomorphometric analysis was performed in eight different regions. Results: New bone percentages were similar in both groups. There were no statistically significant differences. In MG, the overall percentages were 15.6 ± 7.3%, 22.9 ± 6.1%, and 24.9 ± 12.0% after 2, 4, and 8 weeks, respectively. In NMG, the percentages were 11.2 ± 4.5%, 24.1 ± 5.7%, and 24.5 ± 15.7%, respectively. The proportions of new bone in the various regions after 8 weeks were 31 ± 8.9% and 29.9 ± 9.1% in the bone walls region, 25 ± 10.1% and 32.8 ± 9.1% in the submucosa region, 22.6 ± 21.6% and 10.9 ± 11.5 in the middle region, 17.3 ± 14% and 13.4 ± 9.8% in the close-to-window region, and 21.8 ± 11.6%, 19.1 ± 6.4% in the osteotomy region—for MG and NMG, respectively. Conclusions: In both groups the greatest amounts of bone formation occurred near to the pre-existing bone walls, followed by the sub-mucosa region. The smallest amounts were found in the close-to-window region, followed by the central region. The placement of a collagen membrane to cover the osteotomy site did not influence the amount of new bone formation after sinus grafting.
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- 2021
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32. Schneiderian Membrane Collateral Damage Caused by Collagenated and Non-Collagenated Xenografts: A Histological Study in Rabbits.
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Nakajima, Yasushi, Botticelli, Daniele, De Rossi, Ermenegildo Federico, Ferreira Balan, Vitor, Pires Godoy, Eduardo, Ricardo Silva, Erick, and Xavier, Samuel Porfirio
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NASAL mucosa ,XENOGRAFTS ,RABBITS - Abstract
Background: The Schneiderian membrane (SM) that is in contact with biomaterial granules may become thinner and eventually perforate. It has been shown that these events are related to the biomaterial used. Hence, the main aim of the present study was to compare the damaging effects of two xenografts with different resorbability rates on SM. The secondary aim was to evaluate the possible protection from damage offered by a collagen membrane placed adjacent to the SM and by inward displacement of the bone window with the SM during elevation. Methods: Thirty-six albino New Zealand rabbits underwent bilateral sinus elevation. One group of 18 animals received deproteinized bovine bone mineral (DBBM group) and the other received swine-collagenated corticocancellous bone (collagenated group). Moreover, in the DBBM group, the bone window was displaced inward during elevation in one sinus together with the SM. In the collagenated group, a collagen membrane was placed adjacent to the SM in one sinus. Six animals were assessed per period after 2, 4, and 8 weeks. Results: The mean pristine mucosa width ranged between 67 µm and 113 µm, and none had a width of <40 µm. In the 2-week group, the elevated mucosa of the DBBM group presented 59 thinned sites and five perforations, while in the collagenated group, 14 thinned sites and one perforation were observed. Damage to SM decreased in number in the 4-week treatment group. In the 8-week group, the number of thinned sites in the DBBM group increased to 124, and the perforations to 8. In the collagenated group, 7 thinned sites and 1 small perforation were observed. Conclusions: More damage to the Schneiderian membrane was observed in the DBBM group than in the collagenated group. The presence of the inward bone window offered protection from damage to the Schneiderian membrane. [ABSTRACT FROM AUTHOR]
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- 2023
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33. Argon Bioactivation of Implants Installed Simultaneously to Maxillary Sinus Lifting without Graft. An Experimental Study in Rabbits
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Vitor Ferreira Balan, Rafael Arcesio Delgado-Ruiz, Yuki Omori, Daniele Botticelli, Mauro Ferri, and Samuel Porfírio Xavier
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Bone apposition ,Argon ,Maxillary sinus ,business.industry ,Spontaneous Perforation ,Dentistry ,chemistry.chemical_element ,RK1-715 ,Bone healing ,animal study ,Article ,Osseointegration ,histology ,medicine.anatomical_structure ,chemistry ,sinus floor elevation ,medicine ,ODONTOLOGIA ,Schneiderian membrane ,Implant ,bone healing ,business ,General Dentistry ,Sinus (anatomy) - Abstract
Background: The treatment of the surface of titanium implants with argon plasma improved its hydrophilicity and cell adhesion, resulting in higher bone apposition on implant and graft surfaces. The spontaneous perforation over time of the sinus mucosa after sinus augmentation has been documented in experimental studies at both implants and graft particles. The aim of the present study was to evaluate the influence of plasma argon treatment of the implant surface on bone apposition and on the rate of sinus mucosa perforations. Methods: A sinus lifting procedure was performed bilaterally in sixteen rabbits, and implants, either treated with argon plasma or left without treatment (control), were placed simultaneously without grafts. After 8 weeks, histological analyses were carried out. Results: A collapse of the sinus mucosa was observed at all implants. Twenty-four out of thirty-two implants presented sinus mucosa perforations at the apex. Several perforations were also found at the threads. Thinned mucosa sites (width <, 40 µm) were found around almost all implants. About 2.6–2.9 mm of the apical regions of the implant did not present signs of osseointegration and about 1.3 mm were exposed to the sinus cavity. No statistically significant differences were found between plasma and control sites. Conclusions: In conclusion, the sinus mucosa was damaged and perforated by direct contact with treated and non-treated implant surfaces. The treatment of the implant surface with argon plasma did not affect the outcomes.
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- 2021
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34. Sinus Mucosal Damage Triggered by Synthetic or Xenogeneic Bone Substitutes: A Histological Analysis in Rabbits.
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Omori, Yuki, Botticelli, Daniele, Migani, Stefano, Ferreira Balan, Vitor, Pires Godoy, Eduardo, and Xavier, Samuel Porfirio
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BONE substitutes ,RABBITS ,NASAL mucosa ,BONE grafting ,MUCOUS membranes - Abstract
Background: It has been shown in rabbit models that the sinus mucosa in contact with graft particles might experience a progressive thinning and perforations. The phenomenon depends on the graft used. Hence, the aim of the present study was to compare the damaging effects of a synthetic of a xenogeneic graft. Methods: Forty New Zealand rabbits received a bilateral sinus elevation. Both sinuses of twenty rabbits were grafted with a biphasic 60% hydroxyapatite and 40% β-tricalcium phosphate while the other twenty received a deproteinized bovine bone mineral graft. Thinned sites (<40 µm) and perforations on the mucosa in contact with graft particles were evaluated after 2 and 10 weeks (ten animals each period). The width of the pseudostratified epithelium was also measured as control. Results: After 2 weeks of healing, 61 thinned sites were detected in the Synthetic group and 49 in the Xenogeneic group. After 10 weeks, the number of thinned mucosae increased to 79 sites in the Synthetic group (p = 0.222 between periods), and to 114 sites in the Xenogeneic group (p = 0.030 between groups; p = 0.001 between periods). Perforations were few in the 2-week period, two in two sinuses out of 20 in the Synthetic group, and four in two sinuses out of 20 in the Xenogeneic group (p = 0.721). In the 10-week period, the perforations increased to eight in the Synthetic group, distributed in six sinuses out of 20, and to sixteen in the Xenogeneic group, distributed in 11 sinuses out of 20 (p = 0.082). The pseudostratified epithelium presented a reduced width at the thinned sites. Conclusions: The contact with synthetic or xenogeneic grafts will induce thinning and possible perforations of the sinus mucosa. This effect will increase over time, and it is stronger at the xenogeneic than the synthetic graft. [ABSTRACT FROM AUTHOR]
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- 2022
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35. Influence of a collagen membrane positioned subjacent the sinus mucosa following the elevation of the maxillary sinus. A histomorphometric study in rabbits
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Daniele Botticelli, Karol Alí Apaza Alccayhuaman, Takahisa Iida, Niklaus P. Lang, Samuel Porfírio Xavier, Evandro Carneiro Martins Neto, University of Zurich, and Botticelli, Daniele
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0301 basic medicine ,Maxillary sinus ,Sinus Floor Augmentation/methods ,Sinus Floor Augmentation ,610 Medicine & health ,Bone healing ,osteoconductivity ,histology ,10068 Clinic of Reconstructive Dentistry ,03 medical and health sciences ,0302 clinical medicine ,Porcine bone ,medicine ,Animals ,Animal study ,xenograft ,Maxillary Sinus/surgery ,Sinus (anatomy) ,Wound Healing ,Test site ,3504 Oral Surgery ,business.industry ,Collagen membrane ,collagen membrane ,Membranes, Artificial ,Histology ,030206 dentistry ,Anatomy ,Maxillary Sinus ,animal study ,purl.org/pe-repo/ocde/ford#3.02.14 [https] ,030104 developmental biology ,medicine.anatomical_structure ,sinus floor elevation ,Heterografts ,Rabbits ,Collagen ,bone healing ,Oral Surgery ,business ,morphometry - Abstract
OBJECTIVE: To evaluate the healing after elevation of the sinus mucosa when a collagen membrane was placed between the sinus mucosa and a xenograft used as filler. MATERIALS AND METHODS: Eighteen rabbits were used. Sinus mucosa elevation was performed bilaterally, and a collagen membrane was applied subjacent to the sinus mucosa only at a randomly selected test site. At both sites, a collagenated corticocancellous porcine bone was placed within the elevated space and the access window was covered with a collagen membrane. The animals were sacrificed after 2, 4, and 8 weeks of healing, six animals for group. Ground sections were prepared. RESULTS: At the histomorphometric evaluation, the elevated area after 2 and 8 weeks was 11.8 and 8.8 mm(2) at the test, and 10.0 and 5.3 mm(2) at the control sites, respectively. The available area was obtained subtracting the remaining area occupied by the membrane from the elevated area and, after 8 weeks, was 6.7 +/- 0.9 mm(2) . After 8 weeks of healing, the mineralized new bone within the elevated space was 18.2 +/- 5.5% at the test and 26.7 +/- 7.7% at the control sites. Within the available space at the test site, the percentage was 27.3 +/- 7.0% after 8 weeks of healing. At 2 and 8 weeks of healing, within the elevated space, the xenograft proportion was 30.9 +/- 4.4% and 6.9 +/- 2.8% at the test, and 35.2 +/- 7.3% and 9.6 +/- 4.9% at the control sites, respectively. CONCLUSIONS: The placement of a collagen membrane subjacent the sinus mucosa did not reveal any major morphometric difference. The collagen membrane was not completely resorbed after 8 weeks.
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- 2017
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36. New bone ingrowth into β-TCP/HA graft activated with argon plasma: a histomorphometric study on sinus lifting in rabbits
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Shunsuke Baba, Luigi Canullo, Samuel Porfírio Xavier, Kazushige Tanaka, and Daniele Botticelli
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Animal study ,0206 medical engineering ,chemistry.chemical_element ,lcsh:Medicine ,02 engineering and technology ,Bone healing ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Bone formation ,ENXERTO ÓSSEO ,Sinus (anatomy) ,Argon ,Research ,Xenograft ,lcsh:R ,Biomaterial ,Synthetic graft ,030206 dentistry ,020601 biomedical engineering ,Bone ingrowth ,lcsh:RK1-715 ,medicine.anatomical_structure ,Argon plasma ,chemistry ,Sinus floor elevation ,lcsh:Dentistry ,Biomedical engineering ,Protein adsorption - Abstract
Background In a previous experimental study, new bone was found growing within granules of HA/β-TCP. In vitro and experimental studies have shown increased protein adsorption and cell adhesion graft material bioactivated with Argon plasma. The aims of the present experiment were to study new bone ingrowth into β-TCP/HA granules used as filler material for sinus lifting and the influence on the healing of the bioactivation of the graft with argon plasma. Methods Sinus lifting was carried out in 20 rabbits using 60% HA and 40% β-TCP as filler material either bio-activated with argon plasma (plasma group) or left untreated (control group). The antrostomies were closed with collagen membranes. Biopsies representing the healing after 2 and 10 weeks were collected, and ground sections were prepared for histomorphometric analyses. Various regions of the elevated space were analyzed both around (outer bone; OB) and inside (interpenetrating bone network, IBN) the graft particles. Results After 2 weeks of healing, 8.2% and 9.3% (n = 10; p = 0.635) of total new bone (OB + IBN) was found in the plasma and control groups, respectively. Small fractions of IBN were found, spreading from the periphery inward of the graft particles. After 10 weeks of healing, the total new bone was 34.0% in the plasma and 31.3% in Control groups (n = 9; p = 0.594). The respective fractions of IBN were 18.0% and 16.0%. New bone was penetrating from the peripheral regions inside the remnants of graft particles, where it was forming a network of bridges in continuity to the remnants of biomaterial through its porosities. The biomaterial decreased in proportion between 2 and 10 weeks from 52.1 to 28.3% in the plasma group, and from 52.5% to 31.9% in the control group. Conclusion The bio-activation with argon plasma on a synthetic graft composed of 60% HA and 40% β-TCP used as filler material for sinus lifting showed a tendency to improve bone formation; however, the difference with the control group was neither statistically significant nor clinically relevant.
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- 2020
37. Clinical and Histological Healing after Maxillary Sinus Floor Elevation.
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Botticelli, Daniele
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MAXILLARY sinus ,HEALING ,SINUS augmentation ,OPERATIVE surgery ,NASAL mucosa ,BONE substitutes - Abstract
Sinus floor elevation is a surgical procedure that allows for the insertion of the implant in the posterior region of the maxilla in case of insufficient volume of native bone. Several studies have reported a high success rate, and this has helped to spread this surgical procedure around the world. The subject has been extensively studied and this could lead researchers to think that no new scientific evidence can be provided. However, new ideas and discoveries show that research never reaches a conclusion, and that new information can be added all the time. This collection aimed to provide new evidence that could be added to daily clinical work and to provide new ideas for future research. [ABSTRACT FROM AUTHOR]
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- 2022
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38. Maxillary Sinus Floor Augmentation with Two Different Inorganic Bovine Bone Grafts: an Experimental Study in Rabbits.
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FERREIRA BALAN, Vitor, BOTTICELLI, Daniele, PEÑARROCHA-OLTRA, David, Katsuhiko MASUDA, PIRES GODOY, Eduardo, and XAVIER, Samuel Porfirio
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SINUS augmentation ,BONE grafting ,BONE substitutes ,RABBITS - Abstract
Objective: To compare the sequential healing of maxillary sinuses grafted with two different xenogeneic bone substitutes processed at either a low (300°C) or high (1200ºC) temperature. Methods: A sinus augmentation procedure was performed bilaterally in 20 rabbits and two different xenogeneic bone grafts were randomly used to fill the elevated spaces. Healing was studied after 2 and 10 weeks, in 10 rabbits during each period. Results: After 2 weeks of healing, very small amounts of new bone were observed in both groups, and were mainly confined to close to the sinus bone walls and osteotomy edges. After 10 weeks of healing, new bone was found in all regions, with higher percentages in those close to the bone walls and to the osteotomy. In this period of healing, the proportion of new bone in the 300°C group was 20.0% ± 4.3%, and in the 1200ºC group it was 17.2% ± 4.3% (P = 0.162). In the 1200ºC group, translucent, dark fog-like shadows in regions of the grafts were hiding portions of new bone (interpenetrating bone network). Conclusion: Both biomaterials provided conditions that allowed bone growth within the elevated space, confirming that both biomaterials are suitable to be used as a graft for sinus floor augmentation. [ABSTRACT FROM AUTHOR]
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- 2022
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39. Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep
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Giada Ferrante, Joaquin Urbizo Velez, Daniele Botticelli, Stefano Sivolella, Alessandro Perini, and Franco Bengazi
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Animal study ,Maxillary sinus ,Window repositioning ,0206 medical engineering ,Bone plate ,lcsh:Medicine ,Ocean Engineering ,02 engineering and technology ,Sinus floor elevation ,law.invention ,Polylactic membrane ,03 medical and health sciences ,0302 clinical medicine ,law ,Bone window ,medicine ,Bone graft ,Statistical analysis ,Bone ,Sinus (anatomy) ,business.industry ,Research ,lcsh:R ,030206 dentistry ,Anatomy ,020601 biomedical engineering ,lcsh:RK1-715 ,medicine.anatomical_structure ,Cyanoacrylate ,lcsh:Dentistry ,Resorbable membranes ,business - Abstract
The objective of this study was to compare the healing of the augmented sinus at which the antrostomy was covered with a membrane or the repositioned bone plate.Eight sheep underwent bilateral maxillary sinus floor augmentation. The control site was covered with a resorbable membrane, while at the experimental site the bone plate was repositioned, and both were secured with cyanoacrylate. Animals were euthanised after 4 months and histomorphometric analysis was performed.A large amount of the graft appeared to be partially interpenetrated by the newly formed bone. Statistical analysis demonstrated different percentages of the new bone and bone interpenetrated to the graft between test and control site in the close-to-window area respectively 22.1 ± 12.6 vs 7.5 ± 4.5 (P= 0.028) and 66.1 ± 14.7 vs 44.2 ± 15.1 (P= 0.046). Other areas showed no difference in the bone and graft amount. More bone was found at the edges of the antrostomy in the experimental site, without statistical significance. In the centre of the antrostomy, the replaced bony window appeared bonded to the newly formed bone. No remnants and no biological response to cyanoacrylate were observed.The repositioning of the bony window after sinus floor elevation in sheep led to a larger amount of newly formed bone in the close-to-window zone of the grafted area. The bony window appeared partially bonded to the new bone. Newly formed bone was found interpenetrating the graft granules.
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- 2020
40. Comparison of histomorphometry and microCT after sinus augmentation using xenografts of different particle sizes in rabbits
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Shunsuke Baba, Takahisa Iida, Samuel Porfírio Xavier, Katsuhiko Masuda, and Daniele Botticelli
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Maxillary sinus ,Sinus Floor Augmentation ,Bone healing ,Sinus floor elevation ,03 medical and health sciences ,0302 clinical medicine ,Natural bone ,Medicine ,Animals ,Bone formation ,Animal study ,Particle Size ,030223 otorhinolaryngology ,Sinus (anatomy) ,BIOMATERIAIS ,Bone Transplantation ,business.industry ,030206 dentistry ,X-Ray Microtomography ,Maxillary Sinus ,Bovine bone ,medicine.anatomical_structure ,Otorhinolaryngology ,Bone Substitutes ,Heterografts ,Surgery ,Cattle ,Rabbits ,Oral Surgery ,Nuclear medicine ,business - Abstract
The aim of the present experiment was to compare the data on new bone formation measured histologically and microtomographically in maxillary sinuses augmented with a xenograft with higher density and higher mineral content compared with the natural bone. The hypothesis was that histomorphometric and micro-computed tomography (microCT) analyses do not yield similar outcomes when a xenograft with higher density and mineral content compared with the natural bone is used. In 18 rabbits, the maxillary sinus was augmented bilaterally using deproteinized bovine bone mineral (DBBM) xenograft granules of either 0.125–1 mm or 1–2 mm of dimensions. The rabbits were euthanized after 2, 4, and 8 weeks of healing. Comparisons were performed between microCT and histological analyses. After 2 weeks of healing, higher contents of bone were found at the histological compared with the microCT analyses in both sinuses, especially in the middle regions of the grafted sinus. Between 2 and 8 weeks of healing, new bone increased of about 21% at the histological analyses while, at the microCT, increased only about 4%. In the same period, the xenograft proportion decreased from 51.6 ± 4.9 to 45.3 ± 3.3% at the histological analyses while, at the microCT, the xenograft appeared to increase in percentages. Histological and microCT analyses yielded different outcomes when a xenograft with higher density and higher mineral content compared with the natural bone was used.
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- 2020
41. Maxillary Sinus Augmentation Using Ceramic Alloplastic Granules or Paste: An Experimental Study in Rabbits
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Erick Ricardo Silva, Shigeo Fujiwara, M. M. R. Costa, Daniele Botticelli, Yuki Omori, Ofer Moses, and Samuel Porfírio Xavier
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Maxillary sinus ,Dentistry ,Bone healing ,histomorphometry ,Article ,Sinus floor elevation ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animal study ,Bone formation ,General Dentistry ,Sinus (anatomy) ,alloplastic ,business.industry ,Chemistry ,CICATRIZAÇÃO ,Collagen membrane ,collagen membrane ,Biomaterial ,RK1-715 ,030206 dentistry ,animal study ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,sinus floor elevation ,bone healing ,business - Abstract
Background: Due to the lack of data comparing the biological behavior of two formulations, granules and paste, of alloplastic graft from microtomographic and histomorphometric points of view, the aim of the present experiment was to compare the histomorphometric and microtomographic healing of two formulations, i.e., granules (MR sites) or paste (MR-inject sites) of an alloplastic graft composed of a combination of beta-tricalcium phosphate and hydroxyapatite used for maxillary sinus lifting. Methods: A sinus lifting procedure was carried out bilaterally in 20 rabbits, and the elevated space was filled with either paste or granules of an alloplastic material. A collagen membrane was placed on the antrostomy and the animals were euthanized after 2 or 10 weeks, 10 animals each group. Microtomographic and histological analyses were performed. Results: Higher proportions of new bone formation were found at the MR, compared to the MR-inject sites both after 2 weeks (2.65 ± 2.89% vs. 0.08 ± 0.12%, p <, 0.01) and 10 weeks of healing (34.20 ± 13.86 vs. 23.28 ± 10.35%, p = 0.022). Conclusions: It was concluded that new bone formation was faster in the MR sites, compared to the MR-inject. However, a longer time of healing should be allowed to make final conclusions about the efficiency in bone formation of the paste formulation of the biomaterial used in the present study.
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- 2021
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42. Sinus floor elevation outcomes following perforation of the Schneiderian membrane. An experimental study in sheep
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Luigi Canullo, Franco Bengazi, Daniele Botticelli, Joaquin Urbizo Velez, Vittorio Favero, Niklaus P. Lang, University of Zurich, and Botticelli, Daniele
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alloplast ,Calcium Phosphates ,Polyesters ,bone graft ,0206 medical engineering ,Perforation (oil well) ,Dentistry ,Connective tissue ,Sinus Floor Augmentation ,610 Medicine & health ,02 engineering and technology ,Bone healing ,Schneiderian Membrane ,bone ,Sinus floor elevation ,Citric Acid ,histology ,10068 Clinic of Reconstructive Dentistry ,03 medical and health sciences ,Random Allocation ,0302 clinical medicine ,Osteogenesis ,Medicine ,Animals ,Sinus (anatomy) ,Sheep, Domestic ,3504 Oral Surgery ,business.industry ,collagen membrane ,Biomaterial ,Histology ,Membranes, Artificial ,030206 dentistry ,Anatomy ,animal study ,020601 biomedical engineering ,Nasal Mucosa ,medicine.anatomical_structure ,sinus floor elevation ,alloplast, animal study, bone, bone graft, bone healing, collagen membrane, histology, morphometry, sinus floor elevation ,Collagen ,bone healing ,Oral Surgery ,business ,morphometry - Abstract
Objective To assess the influence of a collagen membrane covering a perforation of the sinus (Schneiderian) membrane on the outcome (bone fill) of a sinus floor elevation. Materials and Methods Eighteen Pelibuey sheep were used. The animals underwent sinus floor elevation on both sides of the upper jaw. A perforation of 5 × 4 mm in dimension of the sinus mucosa was performed on both sides and, at a randomly selected test site, a collagen membrane was placed to cover the perforation. A graft of biphasic calcium phosphate (60% HA/40% beta-TCP) was subsequently placed bilaterally, and the access window was closed with a membrane made of polylactic acid and a citric acid ester acetyl. The sacrifices were performed after 2, 4, and 12 weeks of healing. Results After 2 weeks of healing, the augmented volume was filled with biomaterial surrounded by connective tissue and minimal new bone was detected. After 4 weeks of healing, new bone was found mainly in connection with the sinus bony walls with percentages of 18.0 ± 12.9% at the test and 12.3 ± 7.9% at the control sites. After 12 weeks of healing, similar amounts of newly formed bone were found compared to the previous healing period, namely 16.7 ± 8.0% and 13.7 ± 10.1% at the test and control sites, respectively, with the highest amount detected in the bottom of the sinus cavity. The newly formed bone was distributed more evenly within the sinus cavity also including the central areas. The differences between test and control sites did not reach statistical significance. Conclusion Even though there were trends for more bone formation when applying a collagen membrane on a sinus mucosal perforation of relatively small dimensions, this study failed to establish the absolute necessity of such a procedure to achieve bone fill in the sinus cavity.
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- 2016
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43. Bone healing at implants with different surface configurations: an experimental study in dogs
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Niklaus P. Lang, Giovanna Iezzi, Marco Beolchini, José Luis Calvo Guirado, Gerardo Gómez Moreno, Daniele Botticelli, University of Zurich, and Botticelli, Daniele
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Animal study ,Titanium implant ,Dentistry ,Mandible ,02 engineering and technology ,Ridge expansion ,Microsphere ,Random Allocation ,chemistry.chemical_compound ,0302 clinical medicine ,Acid Etching, Dental ,Bone width ,Borates ,Materials Testing ,Titanium ,Bone healing ,3504 Oral Surgery ,Dental Implantation, Endosseous ,021001 nanoscience & nanotechnology ,E.R.E ,Oral Surgery ,0210 nano-technology ,Buccal bony plate ,Histology ,Materials science ,Surface Properties ,Dental implant ,610 Medicine & health ,Implant dentistry ,Osseointegration ,03 medical and health sciences ,10068 Clinic of Reconstructive Dentistry ,Dogs ,Hydrofluoric acid ,Animals ,Bone formation ,Dental Implants ,Wound Healing ,Fluoroboric acid ,business.industry ,Split crest ,030206 dentistry ,Dental Prosthesis Design ,chemistry ,Zirconium ,Bone augmentation ,business - Abstract
This is the peer reviewed version of the following article: Beolchini M, Lang NP, Go mez Moreno G, Iezzi G, Botticelli D, Calvo Guirado JL. Bone healing at implants with different surface configurations: an experimental study in dogs. Clin. Oral Impl. Res. 00, 2015; 1–7., which has been published in final form at 10.1111/clr.12562. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. Objective: To study osseointegration of implants with surface modifications by the use of fluoroboric acid and/or H2O2 installed in conventional sites or sites with circumferential marginal defects. Material and methods: Four implants with different surfaces were used. One basic surface (ZirTi") was sandblasted with zirconium microspheres and acid etched additionally with hydrofluoric acid. A second surface was treated with fluoroboric acid instead of hydrofluoric acid. The remainder of the other two surfaces was additionally treated with H2O2. The edentulous mandibles of 6 foxhound dogs were used to randomly install 8.5-mm-long implants with the different surfaces and to study the histological healing after 1 and 3 months. To study osteoconductivity, additional four recipient sites were prepared with the coronal region being widened so that a 4 mm deep and 0.85 mm wide marginal defect resulted after the placement of the four implants with different surfaces. No filler material or membranes were used, and a fully submerged healing was allowed for 3 months. Results: At the conventional sites, new bone formation ranged between 68.5% and 74.9% after 1 month. After 3 months, bone-to-implant contact ranged from 72.6% at the ZirTi" surface to 84.1% at the fluoroboric acid-treated implants, the difference being statistically significant. At the sites with marginal defects, bone formation ranged from 0.77 mm at the surface treated with fluoroboric acid and H2O2, to 1.93 mm at the surface treated with fluoroboric acid alone. Conclusions: Fluoroboric acid treatment alone of titanium implant surfaces resulted in improved osseointegration and osteoconductivity after 3 months. Odontología
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- 2016
44. Bone healing at bicortically installed implants with different surface configurations. An experimental study in rabbits
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José Luis Calvo Guirado, Daniele Botticelli, Marco Caneva, Silvia Maria Spriano, Niklaus P. Lang, Giovanna Iezzi, University of Zurich, and Botticelli, Daniele
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Animal study ,implant ,Surface Properties ,Dentistry ,610 Medicine & health ,Bone healing ,Metaphysis ,Implant dentistry ,Surface configuration ,Osseointegration ,10068 Clinic of Reconstructive Dentistry ,Random Allocation ,Implants, Experimental ,medicine ,surface ,Animals ,Titanium ,dentistry ,Close contact ,Dental Implants ,Bicortical stabilization ,Wound Healing ,Tibia ,Chemistry ,business.industry ,3504 Oral Surgery ,Surface modified ,Dental Implantation, Endosseous ,Diaphysis ,medicine.anatomical_structure ,Osseintegration ,Dental Prosthesis Design ,Microscopy, Electron, Scanning ,Implant ,Rabbits ,Oral Surgery ,Bone marrow compartment ,business - Abstract
Objective To study the sequential healing at bicortically installed implants with surface modifications by the use of fluoroboric acid and/or H2 O2 . Material and methods Twenty-eight albino New Zealand rabbits were used. Two recipient sites were prepared in the tibiae bilaterally, one in the metaphysis and the second in the diaphysis regions. Four implants with different surface characteristics were randomly installed with bicortical stabilization: (i) sandblasted and acid-etched; (ii) same surface as i, but with a substitution of the hydrofluoric acid with fluoroboric acid; (iii) same surface as i, additionally treated with H2 O2 ; and (iv) same surface modified as ii, additionally treated with H2 O2 . The animals were killed after 5, 8, 15, and 30 days. Ground sections were prepared for histological analyses. Results No statistically significant differences in osseointegration were found among various surfaces at any of the healing periods. A higher degree of osseointegration was observed at the implants placed in the metaphysis compared to those placed in the diaphysis, especially during early healing. A higher degree of osseointegration was found at sites with proximity to compact (cortical) bone when compared to the middle portion of the implants, especially in the diaphysis region. Conclusions Surfaces modified with different acids or H2 O2 resulted in similar osseointegration compared to a standard sandblasted and acid-etched surface. The portion of the bicortically installed implants in close contact with the cortical compartment presented a higher percentage of osseointegration compared with the region in contact with the bone marrow compartment.
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- 2015
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45. Healing at the interface between recipient sites and autologous block bone grafts affixed by either position or lag screw methods: a histomorphometric study in rabbits
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Caneva, Marco, Botticelli, Daniele, Carneiro Martins, Evandro Neto, Caneva, Martina, Lang, Niklaus P, Xavier, Samuel P, University of Zurich, Caneva, Marco, and Caneva, Martina
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10068 Clinic of Reconstructive Dentistry ,3504 Oral Surgery ,600 Technology ,autologous bone grafts ,610 Medicine & health ,histometry ,lag screw ,bone healing ,animal study ,position screw ,morphometry ,bone formation - Abstract
OBJECTIVE To compare the sequential healing at the interface gap occurring between autologous bone grafts and recipient sites using two types of fixation techniques. MATERIAL AND METHODS Twenty-four adult male New Zealand white rabbits were used. Two bone grafts were collected from the calvaria and secured to the lateral aspect of the angle of mandible in each animal. Cortical perforations at the recipient sites were performed. However, no modifications were applied to the graft for its adaptation to the recipient site. Two types of fixation techniques by position or lag screws were applied. This was done by preparing osteotomy holes smaller or larger than the screw diameter, respectively. The animals were sacrificed after 3, 7, 20, and 40 days. RESULTS After 3 days, the distance between the graft and the recipient site was similar between the two different fixations. Due to the anatomical shapes of the recipient sites and grafts, the distance between the two parts was lower in the central region (
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- 2017
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46. Short implants (6 mm) installed immediately into extraction sockets: An experimental study in dogs
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Eriberto Bressan, Stefano Sivolella, Niklaus P. Lang, Zoraya Almagro Urrutia, Daniele Botticelli, and Luiz Antonio Salata
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Dentistry ,hard tissue ,Statistics, Nonparametric ,Surgical Flaps ,Osseointegration ,peri-implant mucosa ,Dogs ,implant dentistry ,Animals ,Medicine ,Tooth Socket ,Dental alveolus ,Dental Implants ,business.industry ,Implant dentistry ,Dental Implantation, Endosseous ,osseointegration ,Soft tissue ,animal study ,Short implants ,Dental Prosthesis Design ,CÃES (EXPERIMENTAÇÃO) ,animal study, extraction socket, implant dentistry, short implants, hard tissue, soft tissues, peri-implant mucosa, osseointegration ,Tooth Extraction ,Pulp (tooth) ,extraction socket ,Implant ,short implants ,Oral Surgery ,business ,soft tissues - Abstract
Aim: To evaluate the effect of implant length (6 mm vs.11 mm) on osseointegration (bone-toimplant contact) of implants installed into sockets immediately after tooth extraction. Material and methods: In six Labrador dogs, the pulp tissue of the mesial roots of 3P3 was removed and the root canals were filled. Flaps were elevated bilaterally, the premolars hemisectioned and the distal roots removed. Recipient sites were prepared in the distal alveolus and a 6 mm or an 11 mm long implant was installed at the test and control sites, respectively. Nonsubmerged healing was allowed. After 4 months of healing, block sections of the implant sites were obtained for histological processing and peri-implant tissue assessment. Results: No statistically significant differences were found between test and control sites both for hard and soft tissue parameters. The bone-to-implant contact evaluated at the apical region of the implants was similar as well. Although not statistically significant, the location of the top of the bony crest at the buccal aspect was more apical in relation to the implant shoulder at the test compared with the control sites (2.0 ± 1.4 and 1.2 ± 1.1 mm, respectively). Conclusions: Shorter implants (6 mm) present with equal osseointegration than do longer implants (11 mm).
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- 2012
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47. Sequential Healing at Implants with Different Configuration and Modified Surfaces: An Experimental Study in the Dog
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Schweikert, M. T., Botticelli, D., Sbricoli, L., Antunes, A., Favero, V., and Salata, L. A.
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Animal study ,Bone healing ,Dental Implants ,Wound Healing ,Histology ,Animal ,Gingiva ,SLActive ,Mandible ,Implant dentistry ,Bone ,Early healing ,Implant surface ,Nonsubmerged healing ,Osseointegration ,Animals ,Dental Prosthesis Design ,Dogs ,Models, Animal ,Random Allocation ,Models - Abstract
To evaluate the peri-implant soft and hard tissue adaptation at implants with different modified surfaces and configurations.Six Beagle dogs were used. Mandibular premolars and first molars were extracted bilaterally. After 3 months, full-thickness flaps were elevated, and two different types of trans-mucosal implants (ICX-Gold®, Medentis Medical GmbH, Dernau, Germany and SLActive®, Institute Straumann, Bern, Switzerland) and two different surfaces were randomly installed in the distal regions of one side of the mandible. Abutments were applied, and a nonsubmerged healing was allowed. After 1 month, the procedures were performed in the other side of the mandible, and after a further month, the animals were sacrificed, biopsies were collected, and ground sections prepared for histological examination.Similar results in marginal bone and soft tissues dimensions were observed after 1 month of healing at the two implant systems used, and no major changes could be observed after 2 months of healing. After 1 month, the percentage of new bone was 69.0% and 68.8% at ICX-Gold and SLActive surfaces, respectively. After 2 months, the percentage of new bone was 67.8% and 71.9% at ICX-Gold Medentis and SLActive surfaces, respectively. No statistically significant differences in osseointegration were found.The two implant systems used resulted in similar osseointegration after 1 and 2 months of healing.
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- 2015
48. Healing of implants installed in over- or under-prepared sites--an experimental study in dogs
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Massimiliano Rea, Gladys González González, Sara Ricci, Francesco Mintrone, Niklaus P. Lang, and Daniele Botticelli
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Insertion torque ,Molar ,Dental Implants ,Wound Healing ,Materials science ,Implant dentistry ,business.industry ,Dental Implantation, Endosseous ,Mandible ,Dentistry ,Bone healing ,Osseointegration ,Surgical Flaps ,Dogs ,Torque ,Tooth Extraction ,Animals ,Animal study ,Bicuspid ,Implant ,Oral Surgery ,business - Abstract
OBJECTIVE To study bone healing at implants installed with different insertion torques. MATERIAL AND METHODS In six Labrador dogs, all mandibular premolars and first molars were extracted. After 4 months of healing, flaps were elevated, and two implant sites were prepared at each side of the mandible. In the right side of the mandible, the distal sites were prepared conventionally, while the mesial sites were over-prepared by 0.2 mm. As a consequence, a final insertion torque of ~30 Ncm at the distal and a minimal insertion torque close to 0 Ncm at the mesial sites were obtained. In the left sides of the mandible, however, the recipient sites were underprepared by 0.3 mm resulting in an insertion torque of ≥ 70 Ncm at both implants. Cover screws were applied, and flaps sutured to fully submerge the experimental sites. After 4 months, the animals were sacrificed and ground sections obtained for histological evaluation. RESULTS The mineralized bone-to-implant contact was in the range of 55.2-62.1%, displaying the highest value at implants with ~30 Ncm insertion torque and the lowest value at the implant sites with close to 0 Ncm insertion torque. No statistically significant differences were revealed. Bone density was in the range of 43.4-54.9%, yielding the highest value at implants with ≥ 70 Ncm insertion torque and the lowest at the implant sites with close to 0 Ncm insertion torque. The difference between the sites of ~30 Ncm and the corresponding ≥ 70 Ncm insertion torque reached statistical significance. CONCLUSIONS Similar amounts of osseointegration were obtained irrespective of the insertion torque applied. Moreover, implants installed in sites with close to 0 Ncm insertion torque may properly osseointegrate as well.
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- 2014
49. The platform switching concept revisited. An experimental study in dogs
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Eriberto Bressan, Sara Ricci, Zoraya Almagro Urrutia, Niklaus P. Lang, Daniele Botticelli, and Boris Corazza
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Molar ,Immediate Dental Implant Loading ,Materials science ,Platform switching ,Dentistry ,hard tissue ,Osseointegration ,Surgical Flaps ,peri-implant mucosa ,animal study ,extraction socket ,immediate implants ,implant configuration ,implant dentistry ,placed immediately into extraction sockets ,soft tissues ,Dogs ,Implants, Experimental ,Premolar ,medicine ,Animals ,Tooth Socket ,Dental Implants ,Wound Healing ,business.industry ,Mandible ,Soft tissue ,Buccal administration ,Root Canal Therapy ,medicine.anatomical_structure ,Dental Prosthesis Design ,Coronal plane ,Oral Surgery ,business - Abstract
Objective To evaluate the influence of the configuration of the marginal aspect of implants placed immediately into extraction sockets on peri-implant hard tissue adaptation. Material and methods In 6 Labrador dogs, endodontic treatments of the mesial roots of 1M1 were performed and the distal roots were removed. 2P2 was extracted as well. Implants were immediately placed in the center of the distal alveoli. Cylindrical straight implants were installed in the right side of the mandible (Control), while, in the left side, implants with a reduced diameter in the coronal portion, yielding an indentation in the surface continuity (Test), were installed. Cover screws were affixed, and the flaps were sutured to allow non-submerged healing. After 4 months of healing, histological slides were obtained for assessments. Results A buccal resorption of 1.58 ± 1.28 and 1.90 ± 1.93 mm at the control and of 0.26 ± 0.90 and 0.14 ± 0.66 mm at the test sites was observed at the premolar and molar regions, respectively. The buccal coronal level of osseointegration was located apically to the margin of the smooth/rough surface border by 2.40 ± 0.90 and 3.70 ± 0.87 mm at the control sites and 1.19 ± 0.45 and 2.16 ± 0.96 mm at the test sites at the premolar and molar sites, respectively. All differences yielded statistical significance. Conclusions The use of implants with a reduced diameter in their coronal aspect may contribute to preservation of the buccal bony crest in a more coronal level compared with conventional implants. Thus, the study confirmed the efficacy of the “platform switching” concept.
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- 2013
50. Deproteinized bovine bone mineral particles and osseointegration of implants without primary bone contact: an experimental study in dogs
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Maria E. Quiñones, Stefano Sivolella, Daniele Botticelli, Luiz Antonio Salata, Niklaus P. Lang, and Eriberto Bressan
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Dense connective tissue ,Molar ,defect ,Materials science ,Surface Properties ,medicine.medical_treatment ,distance osteogenesis ,Dentistry ,Bone healing ,Mandible ,Osseointegration ,Surgical Flaps ,Dogs ,Osteogenesis ,animal study, bone contact, bone healing, contact osteogenesis, defect, deprotei- nized bovine bone mineral, distance osteogenesis, implant dentistry, osseointegration ,medicine ,implant dentistry ,Animals ,Bridge (dentistry) ,Fixation (histology) ,Dental Implants ,Minerals ,Wound Healing ,business.industry ,bone contact ,Dental Implantation, Endosseous ,contact osteogenesis ,animal study ,Primary bone ,Dental Prosthesis Design ,deprotei- nized bovine bone mineral ,Tooth Extraction ,Cattle ,Implant ,Oral Surgery ,bone healing ,business - Abstract
Objectives To evaluate the influence on osseointegration of Deproteinized bovine bone mineral (DBBM) particles used to fill defects of at least 1 mm around implants having no primary contact with bone. Material and methods Premolars and first molars were extracted bilaterally from the mandible of six Labrador dogs. After 3 months of healing, mucoperiosteal full-thickness flaps were elevated, and one recipient site was prepared in the molar region of each hemi-mandible to place implants. These were installed with a deliberate circumferential and periapical space to the bone walls of 1.2 mm. All implants were stabilized with passive fixation plates to maintain the implants in situ and without any contact with the implant bed. The control sites were left to be filled with coagulum, while at the test sites, the residual gap was filled with DBBM. After 3 months of submerged healing, the animals were sacrificed. Ground sections were prepared and analyzed histomorphometrically. Results Mineralized bone-to-implant contact was 4.0% and 3.9% for control and test sites, respectively. The width of the residual defects was 0.48 mm and 0.88 mm at the control and test sites, respectively. The percentage of implant surface covered by a layer of dense connective tissue of 0.12 mm of width on average was 84.9% and 88.5% at the control and test sites, respectively. Conclusion A minor and not predictable degree of contact or distance osteogenesis was obtained on the implant surface when primary contact of the implant surface with the implant bed had deliberately been avoided. DBBM grafting of the artificial gap did not favor osseointegration. Neither did it enhance the ability to bridge the gap with newly formed bone in an artificial defect wider than 1 mm.
- Published
- 2013
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