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Caenis pycnacantha sp. nov. Mature larvae (in alcohol): Body length 3.6���5.4 mm, caudal filaments 3.7���4.9 mm. Body generally pale to slightly pale brown or brown. Vertex dark brown with pale ecdysis suture. Ocelli pale, compound eyes dark black (Figs. 1, 12���13). Mouthparts: labrum slightly concave medially, and with dense short hair and spines on anterior margin and dorsal surface (Fig. 2). Mandibles: with long marginal setae on surface and seven to eight spines on inner margin. Left mandible (Fig. 3): outer incisor with three denticles while inner incisor with two; prostheca clavate, with long fine setae and two sharp bristles at apex. Right mandible (Fig. 4): both of the incisors with two denticles, the apex of prostheca represented by a tuft of branched setae. Maxillae (Fig. 5) with 3 obvious bristles at apex and scattered long, fine setae and spines along inner and outer margin. Maxillary palpi segment 2 is the shortest in the three segments (length ratio of them: 1: 0.7: 1.1); segment 1 and 3 with sparse long hair and short spines along both inner and outer margins while segment 2 with three long setae on outer margin subapically only. Segment 1 and 2 of labial palpi subequal in length, segment 3 shorter than any of them; segment 2 and 3 decorated with dense long setae. Glossae and paraglossae with dense tiny setae at apex (Fig. 6). Hypopharynx shown in figure 7, superlinguae with a row of long hair along free margin (Fig. 7). Thorax: Pronotum expanded outwards slightly, with three irregular pale spots dorsally, a narrow and shallow subanterior ridge located near anterior margin; anterolateral angles of pronotum expanded forwards slightly, lateral margin with sparse hairs. Mesonotum with small expanded anterolateral lobes, a pair of coneshaped pale dots near anterior margin; medial line prominent, forming a longitudinal ridge sometimes. Legs pale brown, with dense setae and spines; femora with blackish brown band apically; forefemur with a row of transverse spine-like setae near apex (Figs. 1, 12); Length ratio of femora: tibiae: tarsi of foreleg = 1.5: 1.0: 1.0; claw single, with 6 denticles at base. Tibiae and tarsi pale brown. Mid- and hindlegs with similar color pattern but without transverse setae on femora; mid claw with 8���10 denticles at base, hind claw with many denticles from base to subapex, basal 3 ones slightly larger. Abdomen: terga 1, 2, 10 darker than others; terga 7���9 with three longitudinal pale stripes, one median, two near middle line (Figs. 1, 12); terga 2���9 with sharp posterolateral projections; a row of strong and acute denticle on posterior margin of each sternum. Apex of sternum 9 with blunt margin. Operculate gill 2 subquadrate; dorsal surface pale-brown, color pattern similar to abdominal terga; Y-shape ranges distinct, with dense setae along free margins and few longer setae on lateral edge; ventral surface of gills 2 with tiny palmate outgrowths near outer and posterior margins (Figs. 14���15). Caudal filaments pale, with sparse setae between segments. Male imago (in alcohol): Body length 2.8 mm, caudal filaments 8.0 mm. Head and thorax dark brown; abdomen pale, with diffused brown marks (Fig. 16). Antennae pale, pedicel 1.5X as long as scape, flagellum expanded at base and tapered to apex (Fig. 8). Ocelli pale, compound eyes dark brown. Forefemur washed with brownish black markings; mid and hind femora pale; forefemur: tibiae: tarsi=1.0: 2.9: 1.9; femora of all legs with slightly pigmented apex; legs with paired claws, one hooked, one blunt. Wing pale, transparent, with brown veins, subcosta robust, yellow-brown (Figs. 9, 16). Genitalia: forceps covered with dense, tiny setae on surface; four spines located on the apex. Penis lobes fused, posterior margin with slight median emargination. Styliger plate pale yellow, with brown markings on the middle of the base (Figs. 10���11, 17��� 18). Caudal filaments pale. Female imago (in alcohol): Body length 2.6 mm, caudal filaments 1.3 mm. Similar to male. Etymology. The epithet pycnacantha is from the Latin pycnacanthus (dense), in reference to the surface of the forceps which are covered with dense, tiny setae. Diagnosis and discussion. To date only six Caenis species have been reported from Chinese Mainland (Eaton 1884; Klap��lek 1905; Ulmer 1936; Zhou et al. 1997, 2000; Gui et al. 1999; Zhou & Zheng 2004). C. pycnacantha sp. nov. is similar to C. nigropunctata (see Ulmer 1940) because both have three longitudinal pale stripes on larval abdominal terga 7���9, and the forceps have dense setae on the surface and spines on the apex. However, they can be separated by the following characters: 1. The larval legs of C. pycnacantha sp. nov. have obvious dark brown markings, which are absent in C. nigropunctata; 2. The larval abdomen of C. nigropunctata is slightly paler than in C. pycnacantha sp. nov., especially that of terga 10; 3. The apical segment of maxillary palpi of C. pycnacantha sp. nov. is slightly longer than second one, but those of C. nigropunctata are subequal; 4.The adult antennae of the new species are pale and the flagellum is slightly expanded at the base; in C. nigropunctata the antennal base is broader. Moreover, in the male imago of C. pycnacantha sp. nov., the forceps have four large spines at apex compared against C. nigropunctata with a tuft of spines on the same position. Kang & Yang (1994, 1996) and Tong & Dudgeon (2002) described totally 10 Caenis species from Chinese Taiwan and Hong Kong area, but all of them are reported from larval stages only and are distinguished by color pattern and/or mandibular seta number or length. It is believed here that those characters can vary in certain level. Without imaginal materials, it is very difficult to compare the new species here to these species. Malzacher (1984) divided the European Caenis species into two lineages based on forceps characteristics: horaria ���lineage (with straight, strongly sclerotised tips of forceps) and macrura -lineage (bent forceps with some bristles or a tuft of long spines on tips). The macrura -lineage is divided further into five species groups. C. pycnacantha sp. nov. can be placed into the macrura -lineage and macrura -group because of the apical spines on the forceps; it is distinguished from other members of the group by the dense setae on the surface of the forceps. Biology. Most nymphs of C. pycnacantha sp. nov. were collected from small pools in streams with sediments of thick leaves and soil. Nymphs were often found under surface of stones in water. This species can be found and collected in most seasons except cold winter (usually from end of November to February). The adults are easily reared from mature nymphal stage, those having dark wing pads can become adults in 2��� 3 days in the rearing tank. However, we did not find living adults during our daytime field collections or at light in the evening although some dead imagos were seen on the water surface during the day. Zhou et al. (2004) reported that in dawn the collect light can attract many caenid adults and subimagos. It is suspected the new species emerges in the early morning too. The width of streams during collection in Zijin Hill was about 1 m and depth of water was less than 40 cm (some places only 10 cm). The streams are often represented by small lentic pools and water flows under the stream bed in some sections. Except after rain, the streams flow slowly, less than 20 cm /s. There is a heavy tree canopy shading most of the stream (Fig. 19). Material examined. Holotype: 1 male, Ying-Tuo village, May 24, 2004, Peng-LI; Paratypes: 24 Nymphs, 6 males 20 females (reared from larvae in lab), same data as for holotype; 12 Nymphs, Ling-Gu-Si Temple, May 24, 2006, Peng-LI; 3 Nymphs, Water-side Pavilion, May 29, 2007, Dong LIU, Hui XIE and Shi-Lei WANG; 10 Nymphs, Ying-Tuo village, May 11, 2008, Shi-Lei WANG, Hui XIE, Yan-Yan JIA and Ping CHEN; 2 Nymphs, Ying-Tuo village, Sep. 22, 2008, Shi-Lei WANG, Yan-Yan JIA and Ping CHEN. All types and specimens are collected from Zijin Hill, Nanjing City, Jiangsu Province, China and deposited in the Mayfly Collection, College of Life Sciences, Nanjing Normal University, China., Published as part of Jia, Yan-Yan, Qin, Jia-Zhang, Ju, Min & Zhou, Chang-Fa, 2010, A new mayfly species of Caenis from headwater of Zijin Hill (Nanjing, Eastern China) (Ephemeroptera: Caenidae), pp. 61-68 in Zootaxa 2535 on pages 61-67, DOI: 10.5281/zenodo.196612, {"references":["Eaton, A. E. (1884) A revisional monograph of recent Ephemeridae or mayflies. Transactions of the Linnaeus Society of Londo n, Second series, Zoology, vol. 3, part 2, 77 - 152.","Klapalek, F. (1905) Plecopteren und Ephemeriden aus Java, gesammelt von Prof. K. Kraepelin 1904. Mitteilungen aus dem Naturhistorischen Museum in Hamburg, 22, 103 - 107.","Ulmer, G. (1936) Neue Chinesische Ephemeropteren, Nebst Ubersicht Uber die bisher aus China bekannten Arten. Peking Natural History Bulletin, 10 (3), 210 - 215.","Zhou, C. F., Gui, H. & Su, C. R. (1997) Three new species of Ephemeroptera (Insecta) from Henan Province of China. Entomotaxonomia, 19, 268 - 272 (in Chinese).","Zhou, C. F., Gui, H. & Sui, C. R. (2000) The first record of the genus Brachycercus in China with description of a new species (Ephemeroptera: Caenidae). Entomologia Sinica, 7, 132 - 134.","Zhou, C. F. & Zheng, L. Y. (2004) A Preliminary Study on the Genus Caenis (Ephemeroptera: Caenidae) form Chinese Mainland, with Description of a New Species. Entomotaxonomia, 26, 1 - 7.","Kang, S. C. & Yang, C. T. (1994) Caenidae of Taiwan (Ephemeroptera). Chinese Journal of Entomology, 14, 93 - 113.","Kang, S. C. & Yang, C. T. (1996) A new species of Caenis Stephens (Ephemeroptera: Caenidae) from Taiwan. Chinese Journal of Entomology, 16, 55 - 59.","Tong, X. L. & Dudgeon, D. (2002) Three new species of the genus Caenis from Hong Kong, China (Ephemeroptera: Caenidae). Journal of Zoological Research, 23 (3), 232 - 238.","Malzacher, P. (1984): Die europaischen Arten der Gattung Caenis Stephens (Insecta: Ephemeroptera). Stuttgarter Beitrage zur Naturkunde, Ser. A., 373 (48), 1 - 48."]}

Cincticostella bifurcata sp. nov. Mature larva: (in ethanol); body length 4.0–9.2 mm. Caudal filaments 3.0–5.5 mm. Body yellowish-brown. Ocelli, clypeus dorsal surface and vertex with various small tubercles and ridges (Figs. 1, 14). Mouthparts: anteromedian emargination of labrum shallow; dorsal labial surface with scattered setae and thick row of submarginal setae, free margins with relatively longer setae; ventral surface densely and irregularly covered with setae (Fig. 2). Mandible with dense row of long setae on dorsal and lateral surface; outer incisor apex trifurcated, inner incisor bifurcated; prostheca represented by tuft of long setae; right mandible with tuft of bristles on inner margin (Figs. 3, 4). Superlinguae of hypopharynx with row of setae on anterior margin, dorsal and ventral surfaces with thick setae; lingua with very sparse and tiny setae on dorsal and ventral surfaces (Fig. 5). Maxilla with two larger spines which ventral margin with several denticulate, four setae on inner margin, bushy golden and long hairs on apex and lateral surface, galea-lacinia with several setae on inner margin near base; maxillary palpi 2 -segmented, basal segment slightly broader, apex of 2 nd segment with constriction (Fig. 6). Labium with round glossae; glossae and paraglossae with setae on margins and dorsal surface; labial palpi 3 -segmented, basal segment length subequal to 2 nd segment, 2 nd segment with setae on margins and dorsal surface, apical segment with no setae and much thinner than other segments; mentum small, submentum with sparse setae and spines on margins, irregular short spines and setae on dorsal surface, but smooth on ventral surface (Fig. 7). Thorax: Pronotum expanded laterally, with anterolateral angles projecting anteriorly, mesonotum projections broad, thoracic nota with various irregular ridges and tubercles, pair of longitudinal ridges and sharp posterior projections between forewing pads (Figs. 1, 14). Femora of all legs flattened, especially those of mid- and hindfemora. Femora of all legs longer than tibiae, and tibiae longer than tarsi. Forefemora with short spines and thick setae on margin, foretibiae and foretarsi with setae on both outer and inner margins(Fig. 8). Midleg and hindleg similar: femora flatted, with longitudinal ridge on dorsal surface, some setae on basal outer margin; outer margin serrate; outer and inner margins with sparse setae near apex of tibiae, whole tarsi with setae on margins (Figs. 9, 10). Claws of all legs with 2 denticles, basal one bigger than distal one (Fig. 11). Abdomen: Pairs of tubercles present on abdominal terga 2-10, those on terga 2, 3, 4, 10 smaller than others; those on terga 5–8 strong, bifurcated apically (Figs. 12, 13); those on tergum 9 sharp-pointed but not bifurcated. Gills present on abdominal terga 3–7, gill 3 without medial transverse band of weakened membrane, dorsal lobe of gills 3–5 similar in shape, gill 6 diminished in size compared to gills 3–5, gill 7 very small and wholly covered by gill 6; ventral lamellae of all gills separated into several lobes, those of gills 3–5 further grouped into two parts, one clear cleft between them; those of gills 6–7 fused together, not bifurcate. Gill 7 located near posterolateral corner. Caudal filaments with tiny spines at articulations. Etymology: bifurcata (feminine), from Latin adjective bifurcatus (bifurcate), indicates tubercles of abdominal terga 5–8 are bifurcated apically on body of larva. Diagnosis: The new species is similar to C. femorata because of their significantly flattened bodies, legs and large projections on the pronotum and mesonotum. The new species can be distinguished from the latter by the following characters: (1) smaller body size; (2) body with many ridges and tubercles dorsally (Fig. 1); (3) mid- and hind femora with smaller serrated free margins (Figs. 9-10); (4) body and legs less flattened; (5) projections of thorax much smaller; (6) abdominal terga 5-8 with bifurcated tubercles(Fig. 13) instead of simple type as the C. femorata. Material examined: holotype: 1 larva (L), Zi-Ma river (29.00978°N, 102.28003°E, elev. 2175m), Conservation station of Zi-Ma river, Conservation area of Li-Zi-Ping, Shi-Mian County, Si-Chuan Province, July 1, 2005, Chang-Fa ZHOU. Paratypes: 2 L, same data as for holotype. 1 L, San-Gang (27.45°N, 117.40°E), Fu-Jian Province, June 7, 1987. 3 L, Mei-Zi river (26 ° 20.905 ΄ N, 100 °01.290΄ E, elev. 2319m, error 9m), Fu- Tian village, Niu-Jie countryside, Er-Yuan County, Da-Li State, Yun-Nan Province, July 8, 2008, Hui XIE, Yan-Yan JIA and Ping CHEN. All types deposited in the Mayfly Collection, College of Life Sciences, Nanjing Normal University, P. R. China 1, habitus of mature larva; 2, labrum (dorsum on left and venter on right); 3, left mandible; 4, right mandible; 5, hypopharynx (dorsum on left and venter on right); 6, right maxilla (ventral); 7, labium (dorsum on left and venter on right); 8, forleg; 9, midleg; 10, hindleg; 11, claw; 12, tubercle present on tergum 8.

Cincticostella bifurcata sp. nov. Mature larva: (in ethanol); body length 4.0���9.2 mm. Caudal filaments 3.0���5.5 mm. Body yellowish-brown. Ocelli, clypeus dorsal surface and vertex with various small tubercles and ridges (Figs. 1, 14). Mouthparts: anteromedian emargination of labrum shallow; dorsal labial surface with scattered setae and thick row of submarginal setae, free margins with relatively longer setae; ventral surface densely and irregularly covered with setae (Fig. 2). Mandible with dense row of long setae on dorsal and lateral surface; outer incisor apex trifurcated, inner incisor bifurcated; prostheca represented by tuft of long setae; right mandible with tuft of bristles on inner margin (Figs. 3, 4). Superlinguae of hypopharynx with row of setae on anterior margin, dorsal and ventral surfaces with thick setae; lingua with very sparse and tiny setae on dorsal and ventral surfaces (Fig. 5). Maxilla with two larger spines which ventral margin with several denticulate, four setae on inner margin, bushy golden and long hairs on apex and lateral surface, galea-lacinia with several setae on inner margin near base; maxillary palpi 2 -segmented, basal segment slightly broader, apex of 2 nd segment with constriction (Fig. 6). Labium with round glossae; glossae and paraglossae with setae on margins and dorsal surface; labial palpi 3 -segmented, basal segment length subequal to 2 nd segment, 2 nd segment with setae on margins and dorsal surface, apical segment with no setae and much thinner than other segments; mentum small, submentum with sparse setae and spines on margins, irregular short spines and setae on dorsal surface, but smooth on ventral surface (Fig. 7). Thorax: Pronotum expanded laterally, with anterolateral angles projecting anteriorly, mesonotum projections broad, thoracic nota with various irregular ridges and tubercles, pair of longitudinal ridges and sharp posterior projections between forewing pads (Figs. 1, 14). Femora of all legs flattened, especially those of mid- and hindfemora. Femora of all legs longer than tibiae, and tibiae longer than tarsi. Forefemora with short spines and thick setae on margin, foretibiae and foretarsi with setae on both outer and inner margins(Fig. 8). Midleg and hindleg similar: femora flatted, with longitudinal ridge on dorsal surface, some setae on basal outer margin; outer margin serrate; outer and inner margins with sparse setae near apex of tibiae, whole tarsi with setae on margins (Figs. 9, 10). Claws of all legs with 2 denticles, basal one bigger than distal one (Fig. 11). Abdomen: Pairs of tubercles present on abdominal terga 2-10, those on terga 2, 3, 4, 10 smaller than others; those on terga 5���8 strong, bifurcated apically (Figs. 12, 13); those on tergum 9 sharp-pointed but not bifurcated. Gills present on abdominal terga 3���7, gill 3 without medial transverse band of weakened membrane, dorsal lobe of gills 3���5 similar in shape, gill 6 diminished in size compared to gills 3���5, gill 7 very small and wholly covered by gill 6; ventral lamellae of all gills separated into several lobes, those of gills 3���5 further grouped into two parts, one clear cleft between them; those of gills 6���7 fused together, not bifurcate. Gill 7 located near posterolateral corner. Caudal filaments with tiny spines at articulations. Etymology: bifurcata (feminine), from Latin adjective bifurcatus (bifurcate), indicates tubercles of abdominal terga 5���8 are bifurcated apically on body of larva. Diagnosis: The new species is similar to C. femorata because of their significantly flattened bodies, legs and large projections on the pronotum and mesonotum. The new species can be distinguished from the latter by the following characters: (1) smaller body size; (2) body with many ridges and tubercles dorsally (Fig. 1); (3) mid- and hind femora with smaller serrated free margins (Figs. 9-10); (4) body and legs less flattened; (5) projections of thorax much smaller; (6) abdominal terga 5-8 with bifurcated tubercles(Fig. 13) instead of simple type as the C. femorata. Material examined: holotype: 1 larva (L), Zi-Ma river (29.00978��N, 102.28003��E, elev. 2175m), Conservation station of Zi-Ma river, Conservation area of Li-Zi-Ping, Shi-Mian County, Si-Chuan Province, July 1, 2005, Chang-Fa ZHOU. Paratypes: 2 L, same data as for holotype. 1 L, San-Gang (27.45��N, 117.40��E), Fu-Jian Province, June 7, 1987. 3 L, Mei-Zi river (26 �� 20.905 �� N, 100 ��01.290�� E, elev. 2319m, error 9m), Fu- Tian village, Niu-Jie countryside, Er-Yuan County, Da-Li State, Yun-Nan Province, July 8, 2008, Hui XIE, Yan-Yan JIA and Ping CHEN. All types deposited in the Mayfly Collection, College of Life Sciences, Nanjing Normal University, P. R. China 1, habitus of mature larva; 2, labrum (dorsum on left and venter on right); 3, left mandible; 4, right mandible; 5, hypopharynx (dorsum on left and venter on right); 6, right maxilla (ventral); 7, labium (dorsum on left and venter on right); 8, forleg; 9, midleg; 10, hindleg; 11, claw; 12, tubercle present on tergum 8., Published as part of Xie, Hui, Jia, Yan-Yan, Chen, Ping, Jacobus, Luke M. & Zhou, Chang-Fa, 2009, Two new Cincticostella species from China with a larval key to species of the genus (Ephemeroptera: Ephemerellidae), pp. 53-61 in Zootaxa 2299 on pages 54-55, DOI: 10.5281/zenodo.191662

Xie, Hui, Jia, Yan-Yan, Chen, Ping, Jacobus, Luke M., Zhou, Chang-Fa (2009): Two new Cincticostella species from China with a larval key to species of the genus (Ephemeroptera: Ephemerellidae). Zootaxa 2299: 53-61, DOI: 10.5281/zenodo.191662