6 results on '"Doty, Jeffrey B."'
Search Results
2. Demonstration of efficient vertical and venereal transmission of dengue virus type-2 in a genetically diverse laboratory strain of Aedes aegypti.
- Author
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Sánchez-Vargas, Irma, Harrington, Laura C., Doty, Jeffrey B., 4thBlack, William C., and Olson, Ken E.
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DENGUE virus genetics ,AEDES aegypti ,SEROTYPES ,EPIDEMICS ,IMMUNOFLUORESCENCE ,RNA - Abstract
Aedes aegypti is the primary mosquito vector of dengue viruses (DENV; serotypes 1–4). Human-mosquito transmission cycles maintain DENV during epidemics but questions remain regarding how these viruses survive when human infections and vector abundance are minimal. Aedes mosquitoes can transmit DENV within the vector population through two alternate routes: vertical and venereal transmission (VT and VNT, respectively). We tested the efficiency of VT and VNT in a genetically diverse laboratory (GDLS) strain of Ae. aegypti orally infected with DENV2 (Jamaica 1409). We examined F1 larvae from infected females generated during the first and second gonotrophic cycles (E1 and E2) for viral envelope (E) antigen by amplifying virus in C6/36 cells and then performing an indirect immunofluorescence assay (IFA). RT-PCR/nested PCR analyses confirmed DENV2 RNA in samples positive by IFA. We observed VT of virus to larvae and adult male progeny and VNT of virus to uninfected virgin females after mating with males that had acquired virus by the VT route. We detected no DENV2 in 30 pools (20 larvae/pool) of F1 larvae following the first gonotrophic cycle, suggesting limited virus dissemination at 7 days post-infection. DENV2 was detected by IFA in 27 of 49 (55%) and 35 of 51 (68.6%) F1 larval pools (20 larvae/pool) from infected E2 females that received a second blood meal without virus at 10 or 21 days post-infection (E2-10d-F1 and E2-21-F1), respectively. The minimum filial infection rates by IFA for E2-10d-F1 and E2-21d-F1 mosquitoes were 1:36 and 1:29, respectively. The VNT rate from E2-10d-F1 males to virgin (uninfected) GDLS females was 31.6% (118 of 374) at 8 days post mating. Twenty one percent of VNT-infected females receiving a blood meal prior to mating had disseminated virus in their heads, suggesting a potential pathway for virus to re-enter the human-mosquito transmission cycle. This is the first report of VNT of DENV by male Ae. aegypti and the first demonstration of sexual transmission in Aedes by naturally infected males. Our results demonstrate the potential for VT and VNT of DENV in nature as mechanisms for virus maintenance during inter-epidemic periods. [ABSTRACT FROM AUTHOR]
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- 2018
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3. Initial pen and field assessment of baits to use in oral rabies vaccination of Formosan ferret-badgers in response to the re-emergence of rabies in Taiwan.
- Author
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Wallace, Ryan M., Lai, Yuching, Doty, Jeffrey B., Chen, Chen-Chih, Vora, Neil M., Blanton, Jesse D., Chang, Susan S., Cleaton, Julie M., and Pei, Kurtis J. C.
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RABIES prevention ,RABIES vaccines ,ZOONOSES ,TAIWANESE people ,FISHING baits ,DISEASES - Abstract
Background: Taiwan had been considered rabies free since 1961, until a newly established wildlife disease surveillance program identified rabies virus transmission within the Formosan ferret-badger (Melogale moschata subaurantiaca) in 2013. Ferret-badgers occur throughout southern China and Southeast Asia, but their ecological niche is not well described. Methodology/Principle findings: As an initial feasibility assessment for potential rabies control measures, field camera trapping and pen assessment of 6 oral rabies vaccine (ORV) baits were conducted in Taiwan in 2013. 46 camera nights were recorded; 6 Formosan ferret-badgers and 14 non-target mammals were sighted. No baits were consumed by ferret-badgers and 8 were consumed by non-target mammals. Penned ferret-badgers ingested 5 of the 18 offered baits. When pen and field trials were combined, and analyzed for palatability, ferret-badgers consumed 1 of 9 marshmallow baits (11.1%), 1 of 21 fishmeal baits (4.8%), 0 of 3 liver baits, and 3 of 3 fruit-flavored baits. It took an average of 261 minutes before ferret-badgers made oral contact with the non-fruit flavored baits, and 34 minutes for first contact with the fruit-based bait. Overall, ferret-badgers sought out the fruit baits 8 times faster, spent a greater proportion of time eating fruit baits, and were 7.5 times more likely to have ruptured the vaccine container of the fruit-based bait. Conclusions/Significance: Ferret-badgers are now recognized as rabies reservoir species in China and Taiwan, through two independent ‘dog to ferret-badger’ host-shift events. Species of ferret-badgers can be found throughout Indochina, where they may be an unrecognized rabies reservoir. Findings from this initial study underscore the need for further captive and field investigations of fruit-based attractants or baits developed for small meso-carnivores. Non-target mammals’ competition for baits, ants, bait design, and dense tropical landscape represent potential challenges to effective ORV programs that will need to be considered in future studies. [ABSTRACT FROM AUTHOR]
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- 2018
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4. Characterization of monkeypox virus infection in African rope squirrels (Funisciurus sp.).
- Author
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Falendysz, Elizabeth A., Lopera, Juan G., Doty, Jeffrey B., Nakazawa, Yoshinori, Crill, Colleen, Lorenzsonn, Faye, Kalemba, Lem’s N., Ronderos, Monica D., Mejia, Andres, Malekani, Jean M., Karem, Kevin, Carroll, Darin S., Osorio, Jorge E., and Rocke, Tonie E.
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MONKEYPOX ,POXVIRUS diseases ,VIRUS diseases ,ZOONOSES ,ENDEMIC diseases ,MICROBIAL virulence - Abstract
Monkeypox (MPX) is a zoonotic disease endemic in Central and West Africa and is caused by Monkeypox virus (MPXV), the most virulent orthopoxvirus affecting humans since the eradication of Variola virus (VARV). Many aspects of the MPXV transmission cycle, including the natural host of the virus, remain unknown. African rope squirrels (Funisciurus spp.) are considered potential reservoirs of MPXV, as serosurveillance data in Central Africa has confirmed the circulation of the virus in these rodent species [,]. In order to understand the tissue tropism and clinical signs associated with infection with MPXV in these species, wild-caught rope squirrels were experimentally infected via intranasal and intradermal exposure with a recombinant MPXV strain from Central Africa engineered to express the luciferase gene. After infection, we monitored viral replication and shedding via in vivo bioluminescent imaging, viral culture and real time PCR. MPXV infection in African rope squirrels caused mortality and moderate to severe morbidity, with clinical signs including pox lesions in the skin, eyes, mouth and nose, dyspnea, and profuse nasal discharge. Both intranasal and intradermal exposures induced high levels of viremia, fast systemic spread, and long periods of viral shedding. Shedding and luminescence peaked at day 6 post infection and was still detectable after 15 days. Interestingly, one sentinel animal, housed in the same room but in a separate cage, also developed severe MPX disease and was euthanized. This study indicates that MPXV causes significant pathology in African rope squirrels and infected rope squirrels shed large quantities of virus, supporting their role as a potential source of MPXV transmission to humans and other animals in endemic MPX regions. [ABSTRACT FROM AUTHOR]
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- 2017
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5. Presumptive risk factors for monkeypox in rural communities in the Democratic Republic of the Congo.
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Quiner, Claire A., Moses, Cynthia, Monroe, Benjamin P., Nakazawa, Yoshinori, Doty, Jeffrey B., Hughes, Christine M., McCollum, Andrea M., Ibata, Saturnin, Malekani, Jean, Okitolonda, Emile, Carroll, Darin S., and Reynolds, Mary G.
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MONKEYPOX virus ,ZOONOSES ,MONKEYPOX ,BUSHMEAT hunting ,SOCIAL status - Abstract
Monkeypox virus (MPXV), a close relative of Variola virus, is a zoonotic virus with an unknown reservoir. Interaction with infected wildlife, bites from peri-domestic animals, and bushmeat hunting are hypothesized routes of infection from wildlife to humans. Using a Risk Questionnaire, performed in monkeypox-affected areas of rural Democratic Republic of the Congo, we describe the lifestyles and demographics associated with presumptive risk factors for MPXV infection. We generated two indices to assess risk: Household Materials Index (HMI), a proxy for socioeconomic status of households and Risk Activity Index (RAI), which describes presumptive risk for animal-to-human transmission of MPXV. Based on participant self-reported activity patterns, we found that people in this population are more likely to visit the forest than a market to fulfill material needs, and that the reported occupation is limited in describing behavior of individuals may participate. Being bitten by rodents in the home was commonly reported, and this was significantly associated with a low HMI. The highest scoring RAI sub-groups were ‘hunters’ and males aged ≥ 18 years; however, several activities involving MPXV-implicated animals were distributed across all sub-groups. The current analysis may be useful in identifying at-risk groups and help to direct education, outreach and prevention efforts more efficiently. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Frameworks for Preventing, Detecting, and Controlling Zoonotic Diseases.
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Shiferaw, Miriam L, Doty, Jeffrey B, Maghlakelidze, Giorgi, Morgan, Juliette, Khmaladze, Ekaterine, Parkadze, Otar, Donduashvili, Marina, Wemakoy, Emile Okitolonda, Muyembe, Jean-Jacques, Mulumba, Leopold, Malekani, Jean, Kabamba, Joelle, Kanter, Theresa, Boulanger, Linda Lucy, Haile, Abraham, Bekele, Abyot, Bekele, Meseret, Tafese, Kasahun, McCollum, Andrea A, and Reynolds, Mary G
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ANIMALS , *HEALTH planning , *NATIONAL health services , *ORGANIZATIONAL change , *PUBLIC health surveillance , *DIAGNOSIS , *ZOONOSES , *PREVENTION - Abstract
Preventing zoonotic diseases requires coordinated actions by government authorities responsible for human and animal health. Constructing the frameworks needed to foster intersectoral collaboration can be approached in many ways. We highlight 3 examples of approaches to implement zoonotic disease prevention and control programs. The first, rabies control in Ethiopia, was implemented using an umbrella approach: a comprehensive program designed for accelerated impact. The second, a monkeypox program in Democratic Republic of the Congo, was implemented in a stepwise manner, whereby incremental improvements and activities were incorporated into the program. The third approach, a pathogen discovery program, applied in the country of Georgia, was designed to characterize and understand the ecology, epidemiology, and pathogenesis of a new zoonotic pathogen. No one approach is superior, but various factors should be taken into account during design, planning, and implementation. [ABSTRACT FROM AUTHOR]
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- 2017
- Full Text
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