1. Differential distribution of the Ca (2+) regulator Pcp4 in the branchial arches is regulated by Hoxa2
- Author
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Shilu Amin, Nicoletta Bobola, Leo A. H. Zeef, Megan Anderson, and Fabiana Luise
- Subjects
Embryology ,lcsh:Medicine ,Fluorescent Antibody Technique ,Developmental Signaling ,Gene Expression ,Mice ,0302 clinical medicine ,Molecular Cell Biology ,Pattern Formation ,Arch ,lcsh:Science ,Cells, Cultured ,Oligonucleotide Array Sequence Analysis ,Regulation of gene expression ,Mice, Knockout ,0303 health sciences ,Multidisciplinary ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Regulation, Developmental ,Genomics ,Signaling in Selected Disciplines ,Chromatin ,Cell biology ,embryonic structures ,Protein Binding ,Research Article ,Signal Transduction ,Chromatin Immunoprecipitation ,animal structures ,Branchial arch ,Nerve Tissue Proteins ,Biology ,03 medical and health sciences ,Developmental Neuroscience ,Animals ,Transcription factor ,030304 developmental biology ,Homeodomain Proteins ,Gene Expression Profiling ,lcsh:R ,Molecular biology ,PCP4 ,Branchial Region ,lcsh:Q ,Genome Expression Analysis ,Chromatin immunoprecipitation ,Organism Development ,030217 neurology & neurosurgery ,Developmental Biology ,Neuroscience - Abstract
Branchial arches are externally visible tissue bands in the head region of all vertebrate embryos. Although initially formed from similar components, each arch will give rise to different head and neck structures. In a screen designed to characterize the molecular control of branchial arch identity in mouse, we identified Pcp4 as a second branchial arch-specific molecular signature. We further show that the transcription factor Hoxa2 binds to Pcp4 chromatin and regulates Pcp4 expression in the second arch. Hoxa2 is also sufficient to induce Pcp4 expression in anterior first arch cells, which are Pcp4-negative.
- Published
- 2013