1. Repair of Osteochondral Defects in a Rabbit Model Using Bilayer Poly(Lactide-co-Glycolide) Scaffolds Loaded with Autologous Platelet-Rich Plasma
- Author
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Song Liu, Jianqun Wu, Jing Niu, Yong-tao Zhang, Bin Yu, and Zhao Wang
- Subjects
macromolecular substances ,02 engineering and technology ,03 medical and health sciences ,chemistry.chemical_compound ,Imaging, Three-Dimensional ,0302 clinical medicine ,Polymethacrylic Acids ,Osteochondrosis ,Animals ,Platelet ,030212 general & internal medicine ,Collagen Type II ,Polyglactin 910 ,Aggrecan ,Whole blood ,Wound Healing ,Poly lactide co glycolide ,Tissue Scaffolds ,Staining and Labeling ,Platelet-Rich Plasma ,Animal Study ,Bilayer ,technology, industry, and agriculture ,Histology ,X-Ray Microtomography ,General Medicine ,Platelet Activation ,021001 nanoscience & nanotechnology ,Immunohistochemistry ,Disease Models, Animal ,PLGA ,chemistry ,Rabbit model ,Joints ,Rabbits ,0210 nano-technology ,Biomedical engineering - Abstract
BACKGROUND To examine the effects of the addition of autologous platelet-rich plasma (PRP) into bilayer poly(lactide-co-glycolide) (PLGA) scaffolds on the reconstruction of osteochondral defects in a rabbit model. MATERIAL AND METHODS Porous PLGA scaffolds were prepared in a bilayered manner to reflect the structure of chondral and subchondral bone. Bone defects, measuring 4 mm in diameter and 4 mm in thickness, were created in both knee joints in 18 healthy New Zealand white rabbits, aged between 120-180 days old. Rabbits were randomly divided into three groups: rabbits with bone defects implanted with bilayer PLGA scaffolds (PLGA group) (N=6); or with bilayer PLGA and autologous PRP (PLGA/PRP group) (N=6); and the untreated group (control group) (N=6). The gross morphology, histology, and immunohistochemistry for the expression of collagen type II and aggrecan were observed at 12 weeks after surgery and compared using a scoring system. Micro-computed tomography (CT) imaging and relative expression of specific genes were also assessed. RESULTS The platelet concentrations in the PRP samples were found to be 4.9 times greater than that of whole blood samples. The total score on gross appearance and histology was greatest in the PLGA/PRP group, as was the expression of collagen II and aggrecan of the neo-tissue. Micro-CT imaging showed that more subchondral bone was formed in the PLGA/PRP group. CONCLUSIONS Bilayer PLGA scaffolds loaded with autologous PRP improve the reconstruction of osteochondral defects in the rabbit model.
- Published
- 2017