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2 results on '"MESH : Immunotherapy"'

1. A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors

Author

Koichi Kawakami, Camila Mejia-Pous, Olivier Gandrillon, Joanna Koszela, José Viñuelas, Claudine Faure, Yoshiko Takahashi, Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Biologie Fonctionnelle, Insectes et Interactions (BF2I), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA), Department of Geophysics [Sendai], Tohoku University [Sendai], LNCC, the CNRS, the UCBL, the Region Rhône Alpes, the IXXI and the ARC, Centre de génétique et de physiologie moléculaire et cellulaire ( CGPhiMC ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Biologie Fonctionnelle, Insectes et Interactions ( BF2I ), Institut National de la Recherche Agronomique ( INRA ) -Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), and Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Université de Lyon-Institut National des Sciences Appliquées ( INSA )

Subjects
MESH : DNA, Complementary, MESH: Immunotherapy, Cell Separation, MESH: Base Sequence, Polymerase Chain Reaction, Retrovirus, MESH: Genetic Vectors, Complementary, MESH: Animals, Transgenes, MESH : Polymerase Chain Reaction, Erythroid Precursor Cells, Cells, Cultured, Genetics, MESH : Immunoglobulin Fc Fragments, biology, Methodology Article, Cell sorting, MESH : Genetic Vectors, Cell biology, Transgenesis, MESH : Immunotherapy, MESH: Immunization, Immunotherapy, SDV:BBM:BM, Stem cell, Biotechnology, Plasmids, Transposable element, Animals, Base Sequence, Cloning, Molecular, DNA, Genetic Vectors, Immunization, Immunoglobulin Fc Fragments, Recombinant Fusion Proteins, Solubility, Cell type, MESH : Cloning, Molecular, MESH : Recombinant Fusion Proteins, lcsh:Biotechnology, Transgene, [ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Transfection, MESH: Immunoglobulin Fc Fragments, lcsh:TP248.13-248.65, MESH: Recombinant Fusion Proteins, MESH: Cloning, Molecular, MESH : Solubility, MESH: Polymerase Chain Reaction, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, MESH : Immunization, MESH: DNA, Complementary, biology.organism_classification, MESH: Solubility, Mutagenesis, Insertional, DNA Transposable Elements, MESH : Base Sequence, MESH : Animals, Chickens
Abstract

Background Stable transgenesis is an undeniable key to understanding any genetic system. Retrovirus-based insertional strategies, which feature several technical challenges when they are used, are often limited to one particular species, and even sometimes to a particular cell type as the infection depends on certain cellular receptors. A universal-like system, which would allow both stable transgene expression independent of the cell type and an efficient sorting of transfected cells, is required when handling cellular models that are incompatible with retroviral strategies. Results We report here on the combination of a stable insertional transgenesis technique, based on the Tol2 transposon system together with the magnetic cell sorting (MACS) technique, which allows specific selection of cells carrying the transgene in an efficient, reliable and rapid way. Conclusion This new Tol2/MACS system leads to stable expression in a culture of primary chicken erythroid cells highly enriched in cells expressing the transgene of interest. This system could be used in a wide variety of vertebrate species.

Published
2009
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2. Delivery of mengovirus-derived RNA replicons into tumoural liver enhances the anti-tumour efficacy of a peripheral peptide-based vaccine

Author

Sylvie van der Werf, Mireille Viguier, Carmen Marchiol, Didier Fradelizi, Anne-Marie Crain, Jean-Pierre Couty, Sarah Boudaly, Marilyne Labasque, Marco Vignuzzi, Catherine Guettier, Nicolas Escriou, Sylvie Gerbaud, Institut Cochin (UMR_S567 / UMR 8104), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5), Université Paris Diderot - Paris 7 (UPD7), Génétique Moléculaire des Virus Respiratoires, Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Pathologie cellulaire : aspects moléculaires et viraux / Pathologie et Virologie Moléculaire, Institut Universitaire d'Hématologie (IUH), Université Paris Diderot - Paris 7 (UPD7)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Anatomie Pathologique, Hôpital Paul Brousse, Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Institut Pasteur [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut Cochin ( UMR_S567 / UMR 8104 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ), Université Paris Diderot - Paris 7 ( UPD7 ), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 ( UPD7 ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'Immunologie Cellulaire et Immunopathologie de l'Ecole Pratique des Hautes Etudes, Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Université Paris Diderot - Paris 7 (UPD7)-Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Male, MESH : Prevalence, MESH: Immunotherapy, medicine.medical_treatment, MESH: Flow Cytometry, Epitope, MESH: Cancer Vaccines, Mice, 0302 clinical medicine, MESH: Liver Neoplasms, Cytotoxic T cell, MESH: Animals, Replicon, MESH : Influenza A Virus, H9N2 Subtype, MESH: Phylogeny, MESH: Treatment Outcome, 0303 health sciences, MESH : Peptides, MESH: Peptides, Liver Neoplasms, MESH : Reverse Transcriptase Polymerase Chain Reaction, Flow Cytometry, MESH : Influenza in Birds, 3. Good health, MESH : Influenza A virus, MESH : Immunotherapy, Oncology, MESH: RNA, Viral, MESH : Animal Migration, Immunotherapy, Carcinoma, Hepatocellular, T cell, Immunology, MESH: Replicon, 03 medical and health sciences, MESH: Species Specificity, MESH: Animals, Wild, MESH : Birds, MESH: Prevalence, Histocompatibility Antigens Class I, Mengovirus, RNA, MESH : Disease Models, Animal, Mice, Inbred C57BL, MESH: Disease Models, Animal, Peptides, MESH: Female, Cancer Research, MESH : Liver Neoplasms, MESH : Mengovirus, MESH: Histocompatibility Antigens Class I, MESH: Reverse Transcriptase Polymerase Chain Reaction, Immunology and Allergy, MESH : Female, MESH: Carcinoma, Hepatocellular, MESH : Animals, Wild, MESH : Cloaca, biology, MESH : Cancer Vaccines, MESH: Animal Migration, medicine.anatomical_structure, Treatment Outcome, MESH: Birds, MESH: Sentinel Surveillance, RNA, Viral, MESH : Histocompatibility Antigens Class I, MESH : Replicon, MESH : Carcinoma, Hepatocellular, MESH : Sentinel Surveillance, MESH : Flow Cytometry, MESH : Male, MESH: Influenza A virus, MESH : Mice, Inbred C57BL, MESH : Treatment Outcome, Cancer Vaccines, MESH: Influenza in Birds, MESH: Mice, Inbred C57BL, MESH : Mice, medicine, MESH : Species Specificity, MESH : RNA, Viral, Animals, MESH : France, MESH: Mice, 030304 developmental biology, MESH : Phylogeny, biology.organism_classification, MESH: Male, MESH: Cloaca, MESH: Influenza A Virus, H9N2 Subtype, MESH: France, Disease Models, Animal, MESH : Animals, CD8, MESH: Mengovirus, 030215 immunology
Abstract

International audience; Hepatocellular carcinoma is a deadly cancer with growing incidence for which immunotherapy is one of the most promising therapeutic approach. Peptide-based vaccines designed to induce strong, sustained CD8+ T cell responses are effective in animal models and cancer patients. We demonstrated the efficacy of curative peptide-based immunisation against a unique epitope of SV40 tumour antigen, through the induction of a strong CD8+ T cell-specific response, in our liver tumour model. However, as in human clinical trials, most tumour antigen epitopes did not induce a therapeutic effect, despite inducing strong CD8+ T cell responses. We therefore modified the tumour environment to enhance peptide-based vaccine efficacy by delivering mengovirus (MV)-derived RNA autoreplicating sequences (MV-RNA replicons) into the liver. The injection of replication-competent RNA replicons into the liver converted partial tumour regression into tumour eradication, whereas non-replicating RNA had no such effect. Replicating RNA replicon injection induced local recruitment of innate immunity effectors (NK and NKT) to the tumour and did not affect specific CD8+ T cell populations or other myelolymphoid subsets. The local delivery of such RNA replicons into tumour stroma is therefore a promising strategy complementary to the use of peripheral peptide-based vaccines for treating liver tumours.

Published
2008
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