1. Pool size estimations for dense-core vesicles in mammalian CNS neurons
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Joris Paul Nassal, Claudia M. Persoon, Margherita Farina, Ruud F. Toonen, Matthijs Verhage, Jan R.T. van Weering, Swati Arora, Jurjen H. Broeke, Natalia Domínguez, Alessandro Moro, Human genetics, Amsterdam Neuroscience - Cellular & Molecular Mechanisms, NCA - Brain mechanisms in health and disease, VU University medical center, and Functional Genomics more...
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0301 basic medicine ,Cell signaling ,Neurite ,Neuropeptide ,Biology ,Hippocampal formation ,Inhibitory postsynaptic potential ,Hippocampus ,Synaptic vesicle ,Article ,General Biochemistry, Genetics and Molecular Biology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,dense-core vesicles ,Neurites ,Animals ,Molecular Biology ,General Immunology and Microbiology ,dense‐core vesicles ,Secretory Vesicles ,General Neuroscience ,Vesicle ,Articles ,Axons ,Corpus Striatum ,Cell biology ,secretion ,release probability ,030104 developmental biology ,nervous system ,Synapses ,Excitatory postsynaptic potential ,pool sizes ,030217 neurology & neurosurgery ,Neuroscience - Abstract
Neuropeptides are essential signaling molecules transported and secreted by dense‐core vesicles (DCVs), but the number of DCVs available for secretion, their subcellular distribution, and release probability are unknown. Here, we quantified DCV pool sizes in three types of mammalian CNS neurons in vitro and in vivo . Super‐resolution and electron microscopy reveal a total pool of 1,400–18,000 DCVs, correlating with neurite length. Excitatory hippocampal and inhibitory striatal neurons in vitro have a similar DCV density, and thalamo‐cortical axons in vivo have a slightly higher density. Synapses contain on average two to three DCVs, at the periphery of synaptic vesicle clusters. DCVs distribute equally in axons and dendrites, but the vast majority (80%) of DCV fusion events occur at axons. The release probability of DCVs is 1–6%, depending on the stimulation. Thus, mammalian CNS neurons contain a large pool of DCVs of which only a small fraction can fuse, preferentially at axons. more...
- Published
- 2018
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