1. Identification of MyD88 as a novel target of miR-155, involved in negative regulation ofHelicobacter pylori-induced inflammation
- Author
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Bin Tang, Bosheng Li, En-Dong Zhu, Zhen Liu, Bin Xiao, Yuan Zhuang, Xuhu Mao, Na Li, Quanming Zou, and Qing-Hua Xie
- Subjects
Myeloid ,Biophysics ,Regulator ,Down-Regulation ,Inflammation ,Biochemistry ,miR-155 ,Immune system ,Downregulation and upregulation ,Structural Biology ,Cell Line, Tumor ,Genetics ,medicine ,Animals ,Humans ,Molecular Biology ,Messenger RNA ,Base Sequence ,Helicobacter pylori ,biology ,MiR-155 ,Interleukin-8 ,Cell Biology ,MyD88 ,biology.organism_classification ,MicroRNAs ,medicine.anatomical_structure ,Protein Biosynthesis ,Myeloid Differentiation Factor 88 ,Cancer research ,medicine.symptom - Abstract
MicroRNA-155 (miR-155) has been implicated as a central regulator of the immune system. We have previously reported that miR-155 negatively regulates Helicobacter pylori (H. pylori)-induced inflammation, but the molecular mechanism of miR-155 regulating the inflammation is not fully clear. Here, we identified myeloid differentiation protein 88 (MyD88) as a target gene of miR-155, and found that miR-155 decreased MyD88 expression at the protein but not the mRNA message level, suggesting that the miR-155-mediated inhibition is a post-transcriptional event. Furthermore, the overexpression of miR-155 led to significantly reduced IL-8 production induced by H. pylori infection. Thus, we have demonstrated that miR-155 can negatively regulate inflammation by targeting a key adaptor molecule MyD88 in inflammatory pathways.
- Published
- 2010