1. Studies on the immunochemistry ofStaphylococcus aureus cell wall: Antigenicity of pentaglycine bridges
- Author
-
Rajinder Singh Ranu
- Subjects
Microbiology (medical) ,Antigenicity ,Staphylococcus ,Immunology ,Glycine ,Heterologous ,Penicillins ,medicine.disease_cause ,Absorption ,Microbiology ,Cell wall ,Cell Wall ,Immunochemistry ,medicine ,Animals ,Immunology and Allergy ,Immunoassay ,Antigens, Bacterial ,biology ,Immune Sera ,Complement Fixation Tests ,Serum Albumin, Bovine ,Hemagglutination Tests ,General Medicine ,Hemagglutination Inhibition Tests ,Peptide Chain Termination, Translational ,Penicillin ,Biochemistry ,Mechanism of action ,Staphylococcus aureus ,biology.protein ,Cattle ,Rabbits ,Antibody ,medicine.symptom ,Peptides ,medicine.drug - Abstract
The specific antigenicity of pentaglycine bridges in cell walls ofS. aureus was investigated. The antistaphylococcal sera (against11 Oeding strains ofS. aureus) were found to contain antibodies of two different specificities against polyglycyl peptides; one type reacted with polyglycyl peptides with a free amino terminus and the other with polyglycyl peptides with a free carboxyl terminus. These antibodies were assayed by passive hemagglutination with tanned sheep red cells sensitized with N-polyglycyl-BSA (containing polyglycyl peptides with a free amino terminus) or with C-polyglycyl-BSA (containing polyglycyl peptides with a free carboxyl terminus). Only the antibodies against N-polyglycyl peptides were absorbed byS. aureus strains and byS. epidermidis. Heterologous bacterial species failed to absorb antibodies of either specificity. Antibodies against polyglycyl peptides were used to confirm the mechanism of action of penicillin onS. aureus cell wall.
- Published
- 1975