1. MiR-361-5p exerts tumor-suppressing functions in gastric carcinoma by targeting syndecan-binding protein
- Author
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Wangfei Wu, Ran Tao, Long Ma, Kun Ye, Daoquan Zhang, Shengyun Wan, Bengli Jia, Bo Qian, and Gang Yu
- Subjects
0301 basic medicine ,Cancer Research ,Syntenins ,Mice, Nude ,Apoptosis ,Syndecan binding ,medicine.disease_cause ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Stomach Neoplasms ,microRNA ,Biomarkers, Tumor ,Tumor Cells, Cultured ,medicine ,Animals ,Humans ,Pharmacology (medical) ,Cell Proliferation ,Pharmacology ,Regulation of gene expression ,Mice, Inbred BALB C ,Cell growth ,Chemistry ,Xenograft Model Antitumor Assays ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Signal transduction ,Carcinogenesis ,Signal Transduction - Abstract
MiR-361-5p, a tumor-related microRNA, has been reported to be implicated in the tumorigenesis and progression of diverse types of human malignancies; however, its role in gastric carcinoma remains unclear. This study aimed to explore the biological role of miR-361-5p in gastric carcinoma and clarify the potential mechanisms involved. In the present study, miR-361-5p was found to be significantly downregulated in both gastric carcinoma tissues and cell lines. Functional studies demonstrated that enhanced expression of miR-361-5p suppressed gastric carcinoma cell proliferation in vitro, inhibited tumor growth in vivo, and induced gastric carcinoma cell apoptosis. Moreover, the tumor-suppressing effects of miR-361-5p in gastric carcinoma were abrogated by the miR-361-5p inhibitor treatment. Notably, syndecan-binding protein was downregulated by miR-361-5p via direct binding to its 3' untranslated region in gastric carcinoma cells. Furthermore, syndecan-binding protein expression was discovered to be markedly upregulated and inversely correlated with miR-361-5p expression in gastric carcinoma tissues. Mechanistic studies revealed that restoring the expression of syndecan-binding protein alleviated miR-361-5p-induced inhibitory effects on proliferation of gastric carcinoma cells. Taken together, these findings suggest that miR-361-5p functions as a tumor suppressor in gastric carcinoma by directly targeting syndecan-binding protein and that miR-361-5p might be a novel therapeutic target for gastric carcinoma.
- Published
- 2020