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3 results on '"Vanessa Maria de Lima Pazine Campanholo"'

1. Chemopreventive activity of grape juice concentrate (G8000TM) on rat colon carcinogenesis induced by azoxymethane

Author

Roseane Mendes Silva, Celina Tizuko Fujiyama Oshima, Daniel Araki Ribeiro, Vanessa Maria de Lima Pazine Campanholo, Ana Paula Ribeiro Paiotti, Nora Manoukian Forones, and Ricardo Artigiani Neto

Subjects
Male, Colorectal cancer, Health, Toxicology and Mutagenesis, medicine.medical_treatment, Azoxymethane, Negative control, Positive control, Pharmacology, Toxicology, chemistry.chemical_compound, otorhinolaryngologic diseases, medicine, Animals, Neoplasm, Vitis, Food science, Rats, Wistar, Saline, Plant Extracts, business.industry, fungi, food and beverages, General Medicine, medicine.disease, Rats, Colon carcinogenesis, Fruit and Vegetable Juices, Gene Expression Regulation, Neoplastic, Disease Models, Animal, Ki-67 Antigen, chemistry, Cyclooxygenase 2, Colonic Neoplasms, Good prognosis, business
Abstract

Colorectal cancer is the third most common cancer worldwide in both sexes, with similar geographic patterns between genders. This neoplasm has good prognosis if the disease is diagnosed at early stages. The aim of this study was to evaluate the effect of red grape juice on the expression of COX-2 and Ki-67 expression following colon carcinogenesis induced by azoxymethane (AOM). Thirty-five rats were randomly distributed into seven groups (n=5 per group): G1: SHAM or negative control received only saline; G2 (positive control): animals received 15 mg/kg AOM; G3: animals received 1% red grape juice 2 weeks before the administration of AOM; G4: animals received 2% red grape juice 2 weeks before the administration of AOM; G5: animals received 1% red grape juice 4 weeks after the last administration of AOM; G6: animals received 2% red grape juice 4 weeks after the last administration of AOM; G7: animals received only 2% red grape juice. COX-2 mRNA expression was reduced in animals treated with 1% red grape juice before AOM induction or 2% red grape juice after AOM induction. COX-2 immunoexpression was also reduced to groups treated with red grape juice at 1% before and after AOM induction or 2% red grape juice after AOM induction. Decreased immunoexpression of Ki-67 positive cells was observed in animals treated with 1% grape juice before AOM-treated animals. Taken together, grape juice concentrate is able to exert some chemopreventive activity on rat colon carcinogenesis.

Published
2015

2. Grape juice concentrate (G8000™) modulates apoptosis but not oxidative stress following rat colon carcinogenesis induced by azoxymethane

Author

Celina Tizuko Fujiyama Oshima, Daniel Araki Ribeiro, Ana Paula Ribeiro Paiotti, Nora Manoukian Forones, Roseane Mendes Silva, Ricardo Artigiani Neto, Vanessa Maria de Lima Pazine Campanholo, Gilles Landman, and Andréa Pittelli Boiago Gollücke

Subjects
Male, Time Factors, Colon, Health, Toxicology and Mutagenesis, medicine.medical_treatment, Azoxymethane, Positive control, Apoptosis, Pharmacology, Toxicology, medicine.disease_cause, chemistry.chemical_compound, otorhinolaryngologic diseases, Animals, Anticarcinogenic Agents, Medicine, Vitis, Food science, Rats, Wistar, Saline, bcl-2-Associated X Protein, Plants, Medicinal, business.industry, fungi, Deoxyguanosine, food and beverages, Colon carcinogenesis, Fruit and Vegetable Juices, Disease Models, Animal, Oxidative Stress, Cell Transformation, Neoplastic, Proto-Oncogene Proteins c-bcl-2, chemistry, 8-Hydroxy-2'-Deoxyguanosine, Fruit, Colonic Neoplasms, business, Oxidative stress, Phytotherapy
Abstract

The aim of this study was to evaluate if grape juice concentrate is able to protect against experimental colon carcinogenesis.For this purpose, a total of 35 male Wistar rats were randomly distributed into seven groups: G1: SHAM animals receiving only saline; G2: animals receiving 15 mg/kg azoxymethane (AOM); G3: animals receiving 1% grape juice concentrate 2 weeks before the administration of AOM; G4: animals receiving 2% grape juice concentrate 2 weeks before the administration of AOM; G5: animals receiving 1% grape juice concentrate 4 weeks after the last administration of AOM; G6: animals receiving 2% grape juice concentrate 4 weeks after the last administration of AOM; G7: animals receiving only 2% grape juice concentrate.The group that received 2% grape juice concentrate before induction with AOM showed the decreased expression of Bcl-2 compared to those animals that were induced by AOM (positive control). Regarding Bax, animals that received grape juice at 2% decreased Bax immunoexpression when compared to AOM group. Furthermore, animals that intake grape juice at 1% after induced by AOM decreased Bax immunoexpression as well. 8-OHdGLI did not show significant statistically differences (p 0.05) among groups.In summary, our results demonstrate that grape juice is able to modulate rat colon carcinogenesis as a result of induction of apoptosis.

Published
2015

3. Oral concentrated grape juice suppresses expression of NF-kappa B, TNF-α and iNOS in experimentally induced colorectal carcinogenesis in Wistar rats

Author

Vanessa Maria de Lima Pazine Campanholo, Roseane Mendes Silva, Ana Paula Ribeiro Paiotti, Tiago Donizetti Silva, Nora Manoukian Forones, Ricardo Artigiani Neto, and Daniel Araki Ribeiro

Subjects
Male, Cancer Research, Epidemiology, Crypt, Azoxymethane, Apoptosis, medicine.disease_cause, Real-Time Polymerase Chain Reaction, law.invention, Andrology, chemistry.chemical_compound, Aberrant Crypt Foci, law, Gene expression, otorhinolaryngologic diseases, medicine, Tumor Cells, Cultured, Animals, Humans, Vitis, Food science, RNA, Messenger, Rats, Wistar, Cell Proliferation, Plant Extracts, Reverse Transcriptase Polymerase Chain Reaction, Tumor Necrosis Factor-alpha, fungi, Public Health, Environmental and Occupational Health, NF-kappa B, food and beverages, Rats, Gene Expression Regulation, Neoplastic, Real-time polymerase chain reaction, Oncology, chemistry, Cyclooxygenase 2, Carcinogens, Tumor necrosis factor alpha, Carcinogenesis, Phytotherapy, Colorectal Neoplasms, Precancerous Conditions, Aberrant crypt foci
Abstract

The aim of this study was to evaluate the effects of grape juice on colon carcinogenesis induced by azoxymethane (AOM) and expression of NF-kB, iNOS and TNF- α. Methods: Forty male Wistar rats were divided into 7 groups: G1, control; G2, 15 mg/kg AOM; G3, 1% grape juice 2 weeks before AOM; G4, 2% grape juice 2 weeks before AOM; G5, 1% grape juice 4 weeks after AOM; G6, 2% grape juice 4 weeks after AOM; G7, 2% grape juice without AOM. Histological changes and aberrant crypt foci (ACF) were studied, while RNA expression of NF-kB, TNF- and iNOS was evaluated by qPCR. Results: The number of ACF was higher in G2, and G4 presented a smaller number of crypts per focus than G5 (p=0.009) and G6. Small ACF (1-3) were more frequent in G4 compared to G2, G5 and G6 (p=0.009, p=0.009 and p=0.041, respectively). RNA expression of NF-kB was lower in G3 and G4 compared to G2 (p=0.004 and p=0.002, respectively). A positive correlation was observed between TNF- α and NF-kB gene expression (p=0.002). In conclusion, the administration of 2% grape juice before AOM reduced the crypt multiplicity, attenuating carcinogenesis. Lower expression of NF-kB was observed in animals exposed to grape juice for a longer period of time, regardless of concentration. Keywords: Grape juice - NF-kappa B - TNF-α - iNOS - carcinogenesis

Published
2015

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