1. Transcostal Histotripsy Ablation in an In Vivo Acute Hepatic Porcine Model
- Author
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Emily A, Knott, Katherine C, Longo, Eli, Vlaisavljevich, Xaiofei, Zhang, John F, Swietlik, Zhen, Xu, Allison C, Rodgers, Annie M, Zlevor, Paul F, Laeseke, Timothy L, Hall, Fred T, Lee, and Timothy J, Ziemlewicz
- Subjects
Liver ,Swine ,Animals ,High-Intensity Focused Ultrasound Ablation ,Female ,Lung ,Magnetic Resonance Imaging - Abstract
To determine whether histotripsy can create human-scale transcostal ablations in porcine liver without causing severe thermal wall injuries along the beam path.Histotripsy was applied to the liver using a preclinical prototype robotic system through a transcostal window in six female swine. A 3.0 cm spherical ablation zone was prescribed. Duration of treatment (75 min) was longer than a prior subcostal treatment study (24 min, 15 s) to minimize beam path heating. Animals then underwent contrast-enhanced MRI, necropsy, and histopathology. Images and tissue were analyzed for ablation zone size, shape, completeness of necrosis, and off-target effects.Ablation zones demonstrated complete necrosis with no viable tissue remaining in 6/6 animals by histopathology. Ablation zone volume was close to prescribed (13.8 ± 1.8 cmTranscostal hepatic histotripsy ablation appears feasible, effective, and no severe injuries were identified in an acute porcine model when prolonged cooling time is added to minimize body wall heating.
- Published
- 2021