1. Development of IL-17-mediated Delayed-Type Hypersensitivity Is Not Affected by Down-Regulation of IL-25 Expression
- Author
-
Akina Ishii, Hideaki Morita, Ko Okumura, Aya Nambu, Susumu Nakae, Keisuke Oboki, Tatsukuni Ohno, Hajime Sudo, Ken Arae, Hirohisa Saito, and Naoki Kajiwara
- Subjects
Male ,lcsh:Immunologic diseases. Allergy ,Cellular differentiation ,medicine.medical_treatment ,Cell ,delayed-type hypersensitivity ,Down-Regulation ,Inflammation ,chemical and pharmacologic phenomena ,Biology ,interleukin-17 family ,Lymphocyte Activation ,Mice ,Th2 Cells ,Immune system ,medicine ,Animals ,Immunology and Allergy ,Hypersensitivity, Delayed ,Cells, Cultured ,Sensitization ,Cell Proliferation ,Mice, Knockout ,Mice, Inbred BALB C ,Cell growth ,Interleukin-17 ,General Medicine ,allergy ,Cytokine ,medicine.anatomical_structure ,helper T cell ,Immunology ,Th17 Cells ,Immunization ,Interleukin 17 ,medicine.symptom ,lcsh:RC581-607 - Abstract
Background IL-25, which is a member of the IL-17 family, induces Th2 cell differentiation and Th2 cytokine production, contributing to induction of Th2-type immune responses and diseases, as a result of which it suppresses Th1- and Th17-type immune responses. Methods To elucidate the role of IL-25 in the pathogenesis of IL-17-mediated delayed-type hypersensitivity (DTH), IL-25-deficient mice were sensitized with methylated BSA (mBSA), and then a DTH reaction was induced by mBSA challenge. mBSA-specific T-cell induction was assessed on the basis of cell proliferation and cytokine production. The DTH reaction was evaluated on the basis of tissue swelling, histology and inflammatory mediator expression. Results IL-25 expression was markedly reduced in local DTH lesions. However, mBSA-specific Th1, Th2 and Th17 cell induction, and the mBSA-induced DTH reaction were comparable in IL-25-deficient and wild-type mice. Conclusions IL-25 is not essential for differentiation of Th1, Th2 and Th17 cells in the sensitization phase or induction of local inflammation in the elicitation phase of the mBSA-induced DTH reaction.
- Published
- 2010