1. Long non-coding RNA MALAT1 exacerbates acute respiratory distress syndrome by upregulating ICAM-1 expression via microRNA-150-5p downregulation
- Author
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Mengying Yao, Gao-Feng Zhao, Wentao Ma, Lihua Xing, Qiuhong Liu, and Weihong Zhang
- Subjects
Lipopolysaccharides ,Aging ,ARDS ,intercellular adhesion molecule-1 ,Necrosis ,Interleukin-1beta ,Down-Regulation ,Apoptosis ,Inflammation ,Lung injury ,Cell Line ,Mice ,Downregulation and upregulation ,Fibrosis ,microRNA ,medicine ,Animals ,Humans ,Gene silencing ,RNA, Small Interfering ,pulmonary microvascular endothelial cells ,lung adenocarcinoma transcript 1 ,Respiratory Distress Syndrome ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,business.industry ,Endothelial Cells ,Cell Biology ,acute respiratory distress syndrome ,medicine.disease ,Up-Regulation ,MicroRNAs ,microRNA-150-5p ,Cancer research ,RNA, Long Noncoding ,medicine.symptom ,business ,Signal Transduction ,Research Paper - Abstract
Acute respiratory distress syndrome (ARDS) is a severe form of acute lung injury in which severe inflammatory responses induce cell apoptosis, necrosis, and fibrosis. This study investigated the role of lung adenocarcinoma transcript 1 (MALAT1) in ARDS and the underlying mechanism involved. The expression of MALAT1, microRNA-150-5p (miR-150-5p), and intercellular adhesion molecule-1 (ICAM-1) was determined in ARDS patients and lipopolysaccharide (LPS)-treated human pulmonary microvascular endothelial cells (HPMECs). Next, the interactions among MALAT1, miR-150-5p, and ICAM-1 were explored. Gain- or loss-of-function experiments in HPMECs were employed to determine cell apoptosis and inflammation. Furthermore, a mouse xenograft model of ARDS was established in order to verify the function of MALAT1 in vivo. MALAT1 and ICAM-1 were upregulated, while miR-150-5p was downregulated in both ARDS patients and LPS-treated HPMECs. MALAT1 upregulated ICAM-1 expression by competitively binding to miR-150-5p. MALAT1 silencing or miR-150-5p overexpression was shown to suppress HPMEC apoptosis, decrease the expressions of pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) and E-selectin in HPMECs, as well as alleviated lung injury in nude mice. These findings demonstrated that MALAT1 silencing can potentially suppress HPMEC apoptosis and alleviate lung injury in ARDS via miR-150-5p-targeted ICAM-1, suggestive of a novel therapeutic target for ARDS. more...
- Published
- 2020
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