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BeibinghongVitis amurensis Ruprhas wide plantation area, high productivity and rich anthocyanin. Common hot-extraction has poor deficiency and destroys anthocyanin severely. For BeibinghongV. amurensis Rupras materials, response surface-optimised electric fields were used, the structure of Beibinghong was observed by SEM, antioxidant activity was measured by DPPH, ABTS and reducing force, the component of anthocyanin was analyzed by HPLC-MS. We found the content of total anthocyanin extracted by pulsed electric fields was 166.65 ± 3.88 mg/100 g.FW. Total anthocyanin from Beibinghong had high antioxidant activity, also contained multiple steady anthocyanin of delphinidin 3-O-glucoside, cyanidin 3-O-glucoside, petunidin 3-O-glucoside, peonidin 3-O-glucoside, malvidin 3-O-glucoside, delphinidin-3-O-(6-O-acetyl) glucoside and delphinidin-3-O-(6-O-p-coumaroyl) glucoside et al. In conclusion, the optimised pulsed electric fields method can quickly and efficiently extract several kinds of anthocyanins fromV. amurensis Rupr. This study promoted the intensive processing ofV. amurensis Ruprand widened the practical application of pulsed electric field technology. [ABSTRACT FROM PUBLISHER]

The purpose of the research was to discuss the Vitis amurensis rich in anthocyanins in wide plantation and low prices. Beibinghong, Gongniangyihao, Shuanghong, Shuangyou and Zuoyouhong were selected as raw materials. The ultrahigh-pressure method was applied in extraction, pH differential method - in total content determination, HPLC - in component analysis, light-heat treatment - in stability analysis, DPPH and ABTS methods - in antioxidation analysis. The total content of anthocyanin in the five varieties of grapes was 166.65 ± 4.63, 67.80 ± 2.87, 398.77 ± 7.45, 100.19 ± 4.04 and 147.62 ± 4.23 mg/100 g, respectively. Besides, there were five kinds of anthocyanosides in hydrolysate, including delphinidin, cyanidin, petunidin, paeonidin and malvidin, with high stability to antioxidation. There are differences between varieties. Vitis amurensis can be used as a new kind of raw material for anthocyanin extraction. [ABSTRACT FROM AUTHOR]