1. Endophytic fungi associated with Sudanese medicinal plants show cytotoxic and antibiotic potential.
- Author
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Khiralla A, Mohamed IE, Tzanova T, Schohn H, Slezack-Deschaumes S, Hehn A, André P, Carre G, Spina R, Lobstein A, Yagi S, and Laurain-Mattar D
- Subjects
- Acetates chemistry, Alternaria chemistry, Byssochlamys chemistry, Byssochlamys isolation & purification, Cell Line, Tumor, Cell Proliferation drug effects, Cell Survival drug effects, Cladosporium chemistry, Cladosporium isolation & purification, DNA, Ribosomal genetics, Endophytes genetics, Endophytes growth & development, Endophytes isolation & purification, Escherichia coli drug effects, Euphorbia microbiology, Fungi genetics, Fungi isolation & purification, Humans, Inhibitory Concentration 50, Methicillin-Resistant Staphylococcus aureus drug effects, Microbial Sensitivity Tests, Plant Leaves microbiology, Sudan, Vernonia microbiology, Anti-Bacterial Agents isolation & purification, Anti-Bacterial Agents pharmacology, Cytotoxins isolation & purification, Cytotoxins pharmacology, Endophytes chemistry, Fungi chemistry, Plants, Medicinal microbiology
- Abstract
In this study, we isolated 15 endophytic fungi from five Sudanese medicinal plants. Each fungal endophytic strain was identified by sequencing of internal transcribed spacer (ITS) regions of rDNA. Ethyl acetate extracts were prepared from each endophyte cultivated in vitro and tested for their respective antibacterial activities and antiproliferative activities against human cancer cells. Antibacterial screening was carried out against two bacterial strains: Gram-negative Escherichia coli and Gram-positive methicillin-resistant Staphylococcus aureus, by the broth dilution method. Cell viability was evaluated by the MTT procedure after exposure of MCF7 breast cancer cells and HT29 or HCT116 human colon adenocarcinoma cells to each endophytic extract. Of interest, Byssochlamys spectabilis isolated from Euphorbia prostata showed cytotoxicity (IC50 = 1.51 ± 0.2 μg mL(-1)) against MCF7 cells, but had a low effect against HT29 or HCT116 cells (IC50 > 20 μg mL(-1)). Cladosporium cladosporioides 2, isolated from Vernonia amygdalina leaves, showed antiproliferative activities against MCF7 cells (IC50 = 10.5 ± 1.5 μg mL(-1)) only. On the other hand, B. spectabilis and Alternaria sp. extract had antibacterial activities against the S. aureus strain. The findings of this work revealed that endophytic fungi associated with medicinal plants from Sudan could be considered as an attractive source of new therapeutic compounds., (© FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2016
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