1. [Antimicrobial Activity of Substances Produced by Trichoderma citrinoviride Strain VKPM F-1228: Optimization of Cultivation and Assessment of Spectrum of Individual Peptaibols].
- Author
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Sadykova VS, Kurakov AV, Korshun VA, Rogozhin EA, Gromovykh TI, Kuvarina AE, and Baranova AA
- Subjects
- Anti-Bacterial Agents pharmacology, Antifungal Agents pharmacology, Aspergillus drug effects, Methicillin-Resistant Staphylococcus aureus drug effects, Microbial Sensitivity Tests, Peptaibols pharmacology, Trichoderma metabolism, Anti-Bacterial Agents isolation & purification, Antifungal Agents isolation & purification, Peptaibols isolation & purification, Trichoderma growth & development
- Abstract
The Trichoderma citrinoviride VKPM F-1228 strain produces a complex of peptide-based antibiotics with antibacterial and antimycotic action. Synthesis of peptaibols is closely related to the conidiogenesis in the culture. The optimal procedure of the strain cultivation for production of peptaibols is stationary growing for 14 days at a temperature of 28 degrees C and pH 7.5 followed by formation of a dense mycelium film on the modified Saburo medium containing 30 gr/l of glucose and 12.5 gr/l of peptone. Eight individual peptaibols were extracted. The spectrum of their activity was estimated with the use of opportunistic bacteria and micromycetes as well as pathogenic clinical aspergilli. Compounds 9, 13, 14, 15 and 16 were shown active against opportunistic fungi and bacteria including methicillin resistant S. aureus, whereas compounds 9, 13 and 14 in addition showed antimycotic activity against clinical aspergilli.
- Published
- 2015