Search

Objectives: Greece is endemic for KPC-encoding Klebsiella pneumoniae; however, until now, reports have referred only to hospital isolates. In this study, seven KPC-encoding K. pneumoniae isolated in private laboratories from non-hospitalised patients were characterised., Methods: Whole-genome sequencing was performed on an Illumina MiniSeq Sequencing System. Multilocus sequence typing (MLST) was performed using a BLAST-based approach, and antimicrobial resistance genes and plasmid replicons were identified using ResFinder and PlasmidFinder, respectively. The Rapid Annotation using Subsystem Technology (RAST) v.2.0 server was used for genome annotation of virulence, pathogenesis and defence genes., Results: Six isolates belonged to the major MLST sequence type 258 (ST258) and one to ST39. The resistome included genes encoding resistance mechanisms to β-lactams, aminoglycosides, quinolones, sulfonamides, trimethoprim, fosfomycin and phenicols, conferring multidrug-resistant phenotypes. Moreover, various genes involved in virulence, pathogenesis and defence have been identified., Conclusions: It is highly probable that these isolates were acquired during previous hospitalisation in Greek hospitals. The presence of KPC-encoding K. pneumoniae in non-hospitalised patients is alarming, although it is not yet possible to assess its actual impact., (Copyright © 2019 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd. All rights reserved.)

Objectives: The emergence and spread of transferable β-lactamases among Enterobacteriaceae is a major problem both to human and veterinary medicine and is an important contributing factor to the development of multidrug-resistant bacterial isolates. In the present study, whole-genome sequencing of a Klebsiella pneumoniae isolate (LKP817909) resistant to first- and second-generation cephalosporins and non-susceptible to fluoroquinolones, isolated from a urine sample of a hospitalised dog, was performed., Methods: Genome sequencing was performed on an Illumina MiniSeq Sequencing System. Multilocus sequence typing (MLST) was performed using a BLAST-based approach, whereas antimicrobial resistance genes and plasmid replicons were identified by ResFinder and PlasmidFinder, respectively. The Rapid Annotation using Subsystem Technology (RAST) server v.2.0 was used for genome annotation., Results: Data analyses revealed the complete resistome of isolate LKP817909, which included the cefotaximase-München-11 (CTX-M-11) extended-spectrum β-lactamase together with 11 other resistance genes. Ten resistance genes were located on plasmids and two on the chromosome., Conclusions: To the best of our knowledge, this is the first detection of a CTX-M-11-producing K. pneumoniae isolated from a canine. The whole genome sequence of the isolate has been deposited at GenBank to serve as a future reference., (Copyright © 2018 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.)