1. Sheng-Mai Yin exerts anti-inflammatory effects on RAW 264.7 cells and zebrafish.
- Author
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Zheng Y, Tian C, Fan C, Xu N, Xiao J, Zhao X, Lu Z, Cao H, Liu J, and Yu L
- Subjects
- Animals, Animals, Genetically Modified, Copper Sulfate, Cytokines metabolism, Disease Models, Animal, Drug Combinations, Inflammation chemically induced, Inflammation immunology, Inflammation metabolism, Lipopolysaccharides, Macrophages immunology, Macrophages metabolism, Mice, NF-KappaB Inhibitor alpha metabolism, NF-kappa B metabolism, Neutrophil Infiltration drug effects, Neutrophils immunology, Neutrophils metabolism, RAW 264.7 Cells, STAT3 Transcription Factor metabolism, Signal Transduction, Zebrafish embryology, Zebrafish genetics, Zebrafish Proteins metabolism, Anti-Inflammatory Agents pharmacology, Drugs, Chinese Herbal pharmacology, Inflammation prevention & control, Inflammation Mediators metabolism, Macrophages drug effects, Neutrophils drug effects
- Abstract
Ethnopharmacological Relevance: Sheng-Mai Yin (SMY), a famous traditional Chinese medicine formula, has been commonly used in China for centuries to treat various diseases, such as inflammation-related diseases. However, the anti-inflammatory activity of SMY and its potential mechanisms still have not yet been clearly understood., Aim of the Study: In this study, we aimed to determine the anti-inflammatory effect of SMY and explore its underlying mechanisms both on RAW 264.7 cells and zebrafish., Materials and Methods: The levels of pro-inflammatory cytokines IL-6 and TNF-α secreted by RAW 264.7 cells were measured by ELISA. The protein expressions of IκBα, p-IκBα (Ser32), STAT3 and p-STAT3 (Tyr705) were determined by Western blotting. And the nuclear translocation of NF-κB p65 in LPS-induced RAW 264.7 macrophage cells was detected by confocal microscopy. Moreover, the in vivo anti-inflammatory effect of SMY and its potential mechanisms were further investigated by survival analysis, hematoxylin-eosin staining (H&E), observation of neutrophil migration and quantitative real-time PCR (qRT-PCR) analysis in zebrafish inflammatory models., Results: SMY reduced the release of IL-6 and TNF-α, inhibited the phosphorylation of IκBα and STAT3 as well as the nuclear translocation of NF-κB p65 in LPS-induced RAW 264.7 cells. Furthermore, the increased survival, decreased infiltration of inflammatory cells and the attenuated migration of neutrophils together suggested the in vivo anti-inflammatory effects of SMY. More importantly, SMY reduced the gene expressions of pro-inflammatory cytokines and suppressed LPS-induced up-regulation of NF-κB, IκBα and STAT3 in zebrafish inflammatory models., Conclusion: SMY exerts significant anti-inflammatory effects with a potential mechanism of inhibiting the NF-κB and STAT3 signal pathways. Our findings suggest a scientific rationale of SMY to treat inflammatory diseases in clinic., (Copyright © 2020. Published by Elsevier B.V.)
- Published
- 2021
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