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Objective: Unfortunately, infectious diseases are still one of the most important health problems around the world. The resistance of pathogenic bacteria to several drugs is an important and growing problem in the treatment of infectious diseases and hospital infections. It seems necessary to identify new compounds that have inhibitory or lethal effects on this bacterium. That's why we decided antimicrobial efficacy. The newly synthesized compound (2-methyl-1,3-dioxo-',11',2',3',5',6',7'a,7-octahydrospiro] inden-'2,3-pyrrolizidine 2-carboxylate (5) on Staphylococcus aureus and Escherichia coli bacteria. Methods: At first, synthesized of methyl'2-methyl-1,3-dioxo-1',1',2',3',5',6', 7',7'a, -octahydrospiro] inden-'2,3-pyrrolizidine 2-Carboxylate was prepared by green chemistry method and with the help of microwave in one pot. Microbial culture media containing control bacteria of Staphylococcus aureus and Escherichia coli were prepared and concentrations of 70 to 110 Landa of the above compound were added to the samples. The antimicrobial property of the samples was determined after 3 days using the disk diffusion method and broth micro dilution method was determined, as well as the minimum inhibitory concentration and bacterial lethality of the samples Results: The minimum inhibitory concentration and bacterial lethality in the above combination against the same standard strain of Escherichia coli bacteria was obtained at the rate of 12.5 µg/mL. The minimum inhibitory and lethal concentration of methyl methacrylate compound against the standard strain of Staphylococcus aureus bacteria was 12.5 and 25 µg/mL, respectively. Conclusion: The use of the above combination was effective in controlling and inhibiting the tested bacteria. [ABSTRACT FROM AUTHOR]

Background and Objectives: Bacterial resistance is an emerging public health problem worldwide. Metallic nanoparticles and nanoalloys open a promising field due to their excellent antimicrobial effects. The aim of the present study was to investigate the antibacterial effects of Ag-Cu nanoalloys, which were biosynthesized by Lactobacillus casei ATCC 39392, on some of the important bacterial pathogens, including Escherichia coli, Burkholderia cepacia, Listeria monocytogenes and Brucella abortus. Materials and Methods: Ag-Cu nanoalloys were synthesized through the microbial reduction of AgNO3 and CuSO4 by Lactobacillus casei ATCC39392. Furthermore, they were characterized by Fourier-Transform Infrared Spectrometer (FTIR) and Field Emission Scanning Electron Microscopy (FESEM) analysis in order to investigate their chemical composition and morphological features, respectively. The minimum inhibitory and minimum bactericidal concentrations of Ag-Cu nanoalloys were determined against each strain. The bactericidal test was conducted on the surface of MHA supplemented with 1, 0.1, and 0.01 µg/µL of the synthesized nanoalloy. The antimicrobial effects of synthesized nanoalloy were compared with ciprofloxacin, ampicillin and ceftazidime as positive controls. Results: Presence of different chemical functional groups, including N-H, C-H, C-N and C-O on the surface of Ag-Cu nanoalloys was recorded by FTIR. FESEM micrographs revealed uniformly distributed nanoparticles with spherical shape and size ranging from 50 to 100 nm. The synthesized Ag-Cu nanoalloys showed antibacterial activity against L. monocytogenes PTCC 1298, E. coli ATCC 25922 and B. abortus vaccine strain. However, no antibacterial effects were observed against B. cepacia ATCC 25416. Conclusion: According to the findings of the present research, the microbially synthesized Ag-Cu nanoalloy demonstrated antibacterial effects on the majority of the bacteria studied even at 0.01 µg/µL. However, complementary investigations should be conducted into the safety of this nanoalloy for in vivo or systemic use. [ABSTRACT FROM AUTHOR]