Search

Abstract Background and Objective: Klebsiella pneumoniae is an opportunistic nosocomial pathogen causing a variety of infections including urinary tract infections, pneumonia, septicemia, wound infections and infections in the intensive care units. Since the ESBL producing Klebsiella pneumoniae strains are increasingly causing urinary tract infections, we aim to assess antibiotic resistance pattern and evaluate the prevalence of ESBL in Klebsiella pneumoniae isolated from urinary tract infections. Material and Methods: this cross-sectional study was conducted on 122 Klebsiella pneumoniae strains collected from Zahedan hospitals. After final identification of isolates, antibiotic susceptibility tests were carried out by using disk diffusion in agar method for 16 antibiotics and ESBL production was determined by the combined disk method. Results: The Klebsiella pneumoniae strains showed susceptibility to imipenem and amikacin ( 94.3%) ,chloramphenicol (88.5%) , gentamicin (81.1%) , ciprofloxacin (80.3%) , cefepime (73%) ,streptomycin (72.1%), nalidixic acid (68%) , tetracycline (65.6%), and cefotaxime, ceftazidime, cefpodoxime (62.3%) . The resistance of strains was seen to nitrofurantoin (53.3%), cotrimoxazole (39.3%), Cefpodoxime (37.7%), cefotaxime (36.9%), ceftriaxone (36.1%), aztreonam (34.4%), ceftazidime (32.8%). Thirty-eight isolates (31.1%) were shown to produce ESBLs. Conclusion: A high rate of resistance was observed to most of the antibiotics among ESBL producing strains therefore, it is important to be careful about the use of antibiotics and identification of ESBL using phenotypic methods. Keywords: Antibiotic Resistance, Extended Spectrum Beta-Lactamases,KlebsiellaPneumoniae, Urinary Tract Infection, Isolate

Background and Objective: Enterococci is gram positive bacteria which is the inhabitants of gastrointestinal tract. Hospital infections and antibiotic resistance to enterococci is increased. This study was done to determine the molecular evaluation of vanA and vanB genes of enterococci isolates resistant to Vancomycin and Teicoplanin. Methods: In this descriptive study, 113 isolates samples were collected and identified according to biochemical test and cultural characteristics in Ali ibn Abi Talib hospital in Zahedan, Iran. Antibiogram test was done to determine antibiotic resistance pattern. E-test strip was used to evaluate the minimum inhibitory of concentration (MIC). PCR was used to detect the vanA and vanB genotype in Vancomycin and Teicoplanin resistance enterococci. Results: 92%, 6.2% and 1.8% of isolated samles collocted from urine, blood culture and pleura fluid, respectively. According to phenotype, 18.6% and 17.69% were resistance to Vancomycin and Teicoplanin, respectively. Resistance was observed in strains of Enterococcus faecalis and Enterococcus faecium. VanA genotype was seen in all of the resistance isolated species. Conclusion: This study showed that strains of Enterococcus faecalis and Enterococcus faecium have more antibiotic resistance to the Vancomycin and Teicoplanin, morever vanA genotype precence in all of resistance isolated samples. [ABSTRACT FROM AUTHOR]