1. Reproductive toxicity of β-diketone antibiotic mixtures to zebrafish (Danio rerio).
- Author
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Wang, Xuedong, Ma, Yan, Liu, Jinfeng, Yin, Xiaohan, Zhang, Zhiheng, Wang, Caihong, Li, Yanyan, and Wang, Huili
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ZEBRA danio ,KETONES ,ANTIBIOTICS ,OXIDATIVE phosphorylation ,GENE expression ,LIGANDS (Biochemistry) - Abstract
So far, few data are available on the reproductive toxicological assessment of β-diketone antibiotics (DKAs), a class of ubiquitous pseudo-persistent pollutant, in zebrafish ( Danio rerio ). Herein, we reported the reproductive effects of DKAs by means of transcriptome analysis (F1-zebrafish), changes in a series of reproductive indices (F0-zebrafish) and histopathological observations. A total of 1170, 983 and 1399 genes were found to be differentially expressed when compared control vs. 6.25 mg/L, control vs. 12.5 mg/L and 6.25 vs. 12.5 mg/L DKA-exposure treatments, respectively. Among three comparison groups, 670, 569 and 821 genes were respectively assigned for GO analyses based on matches with sequences of known functions. In 149 KEGG-noted metabolic pathways, the preferential one was the MAPK (mitogen-activated protein kinase) signaling pathway, followed by oxidative phosphorylation, neuroactive ligand-receptor interaction and so on. By qPCR verification, 6 genes ( c6ast4, igfbp1b, mrpl42, tnnc2, emc4 and ddit4 ) showed consistent gene expression with those identified by transcriptome sequencing. Due to DKA-exposure, the concentrations of plasma estradiol and testosterone, and the gonado-somatic index were significantly dose-dependently declined. Also, DKA-exposure led to declining in zebrafish reproductive capacity, reflecting in fertilization, hatchability and egg production. Histopathological observations demonstrated that zebrafish ovary and testis suffered serious damage after DKA-exposure. The 4-oxo-TEMP signals increased obviously with increasing DKA-exposed concentrations, implying disruption of balance between generation and clearance of 1 O 2 . In summary, DKAs not only produce reproductive toxicological effects on F0-zebrafish, but also result in adverse consequences for growth and development of F1-zebrafish. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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