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The objective of the study was to evaluate the in vitro probiotic potential of 28 Lactobacillus spp. strains, isolated from the vagina of creole dairy cows of the Manabí region -Ecuador-, for its utilization as probiotic in cows with urogenital disorders. The pH decrease and growth capacity at 24 h was determined, and the survival to pH (6, 8 and 9) and temperature (38, 39 and 40 °C), the adherence to hydrocarbons -through hydrophobicity essay of the cell surface-, the self-aggregation capacity, congregation, antimicrobial activity and resistance to 14 antibiotics, were evaluated. Variance analysis was performed on the data with the statistical program INFOSTAT, and to verify the differences among means Duncan's comparison test was used. Fifteen strains grew at different pH and decreased their growth at values equal or lower than 5 and at the optimum temperature of the vaginal ecosystem of the cows, and had high growth capacity at 24 h. The strains LvB-38, 39, 42, 45, 46, 52, 54 and 90 showed capacity of adherence to hydrocarbons (toluene and xylene), higher than 80 %; self-aggregated over 50 %; co-aggregated with Escherichia coli (Migula) Castellani and Chalmers, Staphylococcus aureus Rosenbach and Klebsiella spp., over 75 %; inhibited the growth of these pathogens due to the presence of acids; and were sensitive to ten antimicrobial drugs. It is concluded that the eight selected strains can be considered as candidates to be probiotics, for their utilization in the prevention of urogenital disorders of cows. [ABSTRACT FROM AUTHOR]