1. Development and validation of a competitive ELISA kit for the serological diagnosis of ovine, caprine and bovine brucellosis.
- Author
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Portanti O, Tittarelli M, Di Febo T, Luciani M, Mercante MT, Conte A, and Lelli R
- Subjects
- Agglutination Tests veterinary, Animals, Brucellosis blood, Brucellosis diagnosis, Brucellosis, Bovine blood, Cattle, Complement Fixation Tests veterinary, Enzyme-Linked Immunosorbent Assay standards, Goats, Quality Control, Reference Values, Reproducibility of Results, Rose Bengal, Sensitivity and Specificity, Seroepidemiologic Studies, Sheep, Species Specificity, Swine, Antibodies, Bacterial blood, Brucella immunology, Brucellosis veterinary, Brucellosis, Bovine diagnosis, Enzyme-Linked Immunosorbent Assay veterinary
- Abstract
A competitive ELISA (Brucella-Ab c-ELISA) was standardized and validated for the detection of Brucella antibodies in cattle, sheep and goat sera using a monoclonal antibody (MAb 4B5A) produced against Brucella melitensis biotype 2. The specificity and sensitivity of the assay were 100% to a 67.5% cut-off point (B/Bo%). When compared with an indirect ELISA, the Brucella-Ab c-ELISA did not demonstrate cross-reactions when testing positive sera for antibodies to some Enterobacteriaceae. A comparison was made between the Brucella-Ab c-ELISA and the complement fixation and Rose Bengal tests. Results demonstrated that the Brucella-Ab c-ELISA is a valuable tool for the serological diagnosis of bovine and ovine/caprine brucellosis.
- Published
- 2006
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