1. Antibody mediated fluorescence enhancement of nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC°).
- Author
-
Sellrie F, Lenz C, Andersson A, Wilhelmsson LM, and Schenk JA
- Subjects
- Antibodies, Monoclonal immunology, Antibody Affinity immunology, Antibody Specificity immunology, DNA chemistry, DNA immunology, Enzyme-Linked Immunosorbent Assay methods, Molecular Structure, Oxazines immunology, Reproducibility of Results, Spectrometry, Fluorescence, Antibodies, Monoclonal chemistry, Fluorescence, Immunoassay methods, Oxazines chemistry
- Abstract
We report on the generation and analytical application of the monoclonal antibody G93-ED2 raised against the tricyclic fluorescent nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC°). G93-ED2 is specifically binding this deoxycytidine analogue and was found to raise its fluorescence intensity by a factor of 5. This unique feature makes it a valuable tool in fluorescence dependent immunoassays. G93-ED2 was successfully applied in a homogeneous fluorescence quenching immunoassay (DNA-Q) for the sequence specific determination of DNA., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF