1. Generation of monoclonal antibodies to murine IL-1 beta and demonstration of IL-1 in vivo.
- Author
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Hogquist KA, Nett MA, Sheehan KC, Pendleton KD, Schreiber RD, and Chaplin DD
- Subjects
- Animals, Antibody Specificity, Base Sequence, Escherichia coli metabolism, Interleukin-1 biosynthesis, Interleukin-1 immunology, Mice, Molecular Sequence Data, Precipitin Tests, Recombinant Proteins biosynthesis, Recombinant Proteins isolation & purification, Antibodies, Monoclonal biosynthesis, Interleukin-1 analysis
- Abstract
The role of murine IL-1 beta in vitro and in vivo has not been defined. We describe here the production of neutralizing and immunoprecipitating mAb and polyclonal antibodies specific for murine IL-1 beta and their application to a characterization of the murine IL-1 beta protein. Immunization of either hamsters or rabbits with the recombinant mature form of murine IL-1 beta emulsified in CFA elicited antisera and hamster mAb that only recognized denatured IL-1 beta. In contrast, immunization with rIL-1 beta adsorbed to alum resulted in the generation of neutralizing and immunoprecipitating rabbit and hamster antisera and hamster mAb. All of the mAb recognize both the pro-form of IL-1 beta and the mature bioactive form produced by cultures of murine peritoneal macrophages. Using these antibodies, we demonstrate that approximately half of the IL-1 activity present in supernatants of LPS-treated cultured mouse macrophages is composed of IL-1 beta. Additionally, IL-1 beta as well as IL-1 alpha can be detected in the plasma of LPS-treated mice. These studies, therefore, demonstrate the production of IL-1 beta both in vitro and in vivo.
- Published
- 1991